Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea
Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bi...
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| Veröffentlicht in: | Microbial ecology 2017-02, Vol.73 (2), p.338-352 |
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| creator | Sarmiento-Vizcaíno, Aida González, Verónica Braña, Alfredo F. Palacios, Juan J. Otero, Luis Fernández, Jonathan Molina, Axayacatl Kulik, Andreas Vázquez, Fernando Acuña, José L. García, Luis A. Blanco, Gloria |
| description | Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest. |
| doi_str_mv | 10.1007/s00248-016-0845-2 |
| format | Article |
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Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-016-0845-2</identifier><identifier>PMID: 27614749</identifier><language>eng</language><publisher>New York: Springer Science + Business Media</publisher><subject>Actinobacteria ; Actinobacteria - chemistry ; Actinobacteria - classification ; Actinobacteria - genetics ; Actinobacteria - isolation & purification ; Animals ; Annelida ; Anthozoa - microbiology ; Anti-Bacterial Agents - pharmacology ; Antineoplastic Agents - pharmacology ; Arthropoda ; Bacteria ; Bacteria - drug effects ; Base Sequence ; Bioactive compounds ; Bioassays ; Biodiversity ; Biological Products - chemistry ; Biological Products - isolation & purification ; Biological Products - pharmacology ; Biomedical and Life Sciences ; Bioprospecting ; Biotechnology ; Canyons ; Cell Line, Tumor - drug effects ; Cell Survival - drug effects ; Chromatography, High Pressure Liquid ; Classification ; Cnidaria ; Coral Reefs ; Deep sea ; DNA, Bacterial ; Echinodermata ; Ecology ; Ecosystem ; ENVIRONMENTAL MICROBIOLOGY ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Genes, Bacterial ; Geoecology/Natural Processes ; Invertebrates - microbiology ; Life Sciences ; Liquid chromatography ; Marine ; Marine Biology ; Marine ecosystems ; Mass spectrometry ; Metabolites ; Microbial Ecology ; Microbiology ; Micromonospora ; Mollusca ; Mollusks ; Nature Conservation ; Phylogeny ; Plant Extracts ; Porifera ; Pseudonocardia ; RNA, Ribosomal, 16S - genetics ; Seawater ; Secondary Metabolism ; Shellfish ; Sipuncula ; Spain ; Streptomyces ; Streptomyces - classification ; Streptomyces - isolation & purification ; Water Quality/Water Pollution</subject><ispartof>Microbial ecology, 2017-02, Vol.73 (2), p.338-352</ispartof><rights>Springer Science+Business Media New York 2016</rights><rights>Microbial Ecology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3422-26a71d1aaf94bdf5185758a28297a8a53bf6ac4a7234c09038f9fac11b7c4b6a3</citedby><cites>FETCH-LOGICAL-c3422-26a71d1aaf94bdf5185758a28297a8a53bf6ac4a7234c09038f9fac11b7c4b6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48723404$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48723404$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27614749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sarmiento-Vizcaíno, Aida</creatorcontrib><creatorcontrib>González, Verónica</creatorcontrib><creatorcontrib>Braña, Alfredo F.</creatorcontrib><creatorcontrib>Palacios, Juan J.</creatorcontrib><creatorcontrib>Otero, Luis</creatorcontrib><creatorcontrib>Fernández, Jonathan</creatorcontrib><creatorcontrib>Molina, Axayacatl</creatorcontrib><creatorcontrib>Kulik, Andreas</creatorcontrib><creatorcontrib>Vázquez, Fernando</creatorcontrib><creatorcontrib>Acuña, José L.</creatorcontrib><creatorcontrib>García, Luis A.</creatorcontrib><creatorcontrib>Blanco, Gloria</creatorcontrib><title>Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><addtitle>Microb Ecol</addtitle><description>Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest.</description><subject>Actinobacteria</subject><subject>Actinobacteria - chemistry</subject><subject>Actinobacteria - classification</subject><subject>Actinobacteria - genetics</subject><subject>Actinobacteria - isolation & purification</subject><subject>Animals</subject><subject>Annelida</subject><subject>Anthozoa - microbiology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Arthropoda</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Base Sequence</subject><subject>Bioactive compounds</subject><subject>Bioassays</subject><subject>Biodiversity</subject><subject>Biological Products - chemistry</subject><subject>Biological Products - isolation & purification</subject><subject>Biological Products - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Bioprospecting</subject><subject>Biotechnology</subject><subject>Canyons</subject><subject>Cell Line, Tumor - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Classification</subject><subject>Cnidaria</subject><subject>Coral Reefs</subject><subject>Deep sea</subject><subject>DNA, Bacterial</subject><subject>Echinodermata</subject><subject>Ecology</subject><subject>Ecosystem</subject><subject>ENVIRONMENTAL MICROBIOLOGY</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Genes, Bacterial</subject><subject>Geoecology/Natural Processes</subject><subject>Invertebrates - microbiology</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Marine</subject><subject>Marine Biology</subject><subject>Marine ecosystems</subject><subject>Mass spectrometry</subject><subject>Metabolites</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Micromonospora</subject><subject>Mollusca</subject><subject>Mollusks</subject><subject>Nature Conservation</subject><subject>Phylogeny</subject><subject>Plant Extracts</subject><subject>Porifera</subject><subject>Pseudonocardia</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Seawater</subject><subject>Secondary Metabolism</subject><subject>Shellfish</subject><subject>Sipuncula</subject><subject>Spain</subject><subject>Streptomyces</subject><subject>Streptomyces - classification</subject><subject>Streptomyces - isolation & purification</subject><subject>Water Quality/Water Pollution</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkctu1DAUhi0EokPhAViALLFhE_A1dpbVtEClSoxUkNhFJx6n41FiD7anUl6HHc_Bi-GQUiEWiJUv5zuf5fMj9JySN5QQ9TYRwoSuCK0rooWs2AO0ooKzimrx5SFaEdLIitdMn6AnKe0Joapm_DE6YaqmQolmhb5tdhBHMGEIN87AgDchW59d2YUeb3ZTubfe5rk2TPjc3dqYLD4z2fnQgck2OsCXKQyQ7Rb3MYz43NpDdW0Br0MsngsT0pSyHdOszDuLr4_dCNH54rl1w4_vCa_BT8Fj53_VyylDV8QeF81T9KiHIdlnd-sp-vzu4tP6Q3X18f3l-uyqMlwwVrEaFN1SgL4R3baXVEslNTDNGgUaJO_6GowAxbgwpCFc900PhtJOGdHVwE_R68V7iOHr0abcji4ZOwzgbTimlupac1IT1vwHKhslKJOqoK_-QvfhGH35yCyUTAqiZooulIkhpWj79hBdmdHUUtLOWbdL1m3Jup2zblnpeXlnLuO02_uO3-EWgC1AKiV_Y-MfT__D-mJp2qcc4r1U6HluRPCfX-O_sQ</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Sarmiento-Vizcaíno, Aida</creator><creator>González, Verónica</creator><creator>Braña, Alfredo F.</creator><creator>Palacios, Juan J.</creator><creator>Otero, Luis</creator><creator>Fernández, Jonathan</creator><creator>Molina, Axayacatl</creator><creator>Kulik, Andreas</creator><creator>Vázquez, Fernando</creator><creator>Acuña, José L.</creator><creator>García, Luis A.</creator><creator>Blanco, Gloria</creator><general>Springer Science + Business Media</general><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20170201</creationdate><title>Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea</title><author>Sarmiento-Vizcaíno, Aida ; González, Verónica ; Braña, Alfredo F. ; Palacios, Juan J. ; Otero, Luis ; Fernández, Jonathan ; Molina, Axayacatl ; Kulik, Andreas ; Vázquez, Fernando ; Acuña, José L. ; García, Luis A. ; Blanco, Gloria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3422-26a71d1aaf94bdf5185758a28297a8a53bf6ac4a7234c09038f9fac11b7c4b6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Actinobacteria</topic><topic>Actinobacteria - chemistry</topic><topic>Actinobacteria - classification</topic><topic>Actinobacteria - genetics</topic><topic>Actinobacteria - isolation & purification</topic><topic>Animals</topic><topic>Annelida</topic><topic>Anthozoa - microbiology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Arthropoda</topic><topic>Bacteria</topic><topic>Bacteria - drug effects</topic><topic>Base Sequence</topic><topic>Bioactive compounds</topic><topic>Bioassays</topic><topic>Biodiversity</topic><topic>Biological Products - chemistry</topic><topic>Biological Products - isolation & purification</topic><topic>Biological Products - pharmacology</topic><topic>Biomedical and Life Sciences</topic><topic>Bioprospecting</topic><topic>Biotechnology</topic><topic>Canyons</topic><topic>Cell Line, Tumor - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Classification</topic><topic>Cnidaria</topic><topic>Coral Reefs</topic><topic>Deep sea</topic><topic>DNA, Bacterial</topic><topic>Echinodermata</topic><topic>Ecology</topic><topic>Ecosystem</topic><topic>ENVIRONMENTAL MICROBIOLOGY</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Genes, Bacterial</topic><topic>Geoecology/Natural Processes</topic><topic>Invertebrates - 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Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from corals, phylum Cnidaria, and some specimens of phyla Echinodermata, Porifera, Annelida, Arthropoda, Mollusca and Sipuncula. As determined by 16S rRNA sequencing and phylogenetic analyses, all isolates belong to the phylum Actinobacteria, mainly to the Streptomyces genus and also to Micromonospora, Pseudonocardia and Myceligenerans. Production of bioactive compounds of pharmacological interest was investigated by high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS) techniques and subsequent database comparison. Results reveal that deep-sea isolated Actinobacteria display a wide repertoire of secondary metabolite production with a high chemical diversity. Most identified products (both diffusible and volatiles) are known by their contrasted antibiotic or antitumor activities. Bioassays with ethyl acetate extracts from isolates displayed strong antibiotic activities against a panel of important resistant clinical pathogens, including Gram-positive and Gram-negative bacteria, as well as fungi, all of them isolated at two main hospitals (HUCA and Cabueñes) from the same geographical region. The identity of the active extracts components of these producing Actinobacteria is currently being investigated, given its potential for the discovery of pharmaceuticals and other products of biotechnological interest.</abstract><cop>New York</cop><pub>Springer Science + Business Media</pub><pmid>27614749</pmid><doi>10.1007/s00248-016-0845-2</doi><tpages>15</tpages></addata></record> |
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| ispartof | Microbial ecology, 2017-02, Vol.73 (2), p.338-352 |
| issn | 0095-3628 1432-184X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1868306029 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | Actinobacteria Actinobacteria - chemistry Actinobacteria - classification Actinobacteria - genetics Actinobacteria - isolation & purification Animals Annelida Anthozoa - microbiology Anti-Bacterial Agents - pharmacology Antineoplastic Agents - pharmacology Arthropoda Bacteria Bacteria - drug effects Base Sequence Bioactive compounds Bioassays Biodiversity Biological Products - chemistry Biological Products - isolation & purification Biological Products - pharmacology Biomedical and Life Sciences Bioprospecting Biotechnology Canyons Cell Line, Tumor - drug effects Cell Survival - drug effects Chromatography, High Pressure Liquid Classification Cnidaria Coral Reefs Deep sea DNA, Bacterial Echinodermata Ecology Ecosystem ENVIRONMENTAL MICROBIOLOGY Gas chromatography Gas Chromatography-Mass Spectrometry Genes, Bacterial Geoecology/Natural Processes Invertebrates - microbiology Life Sciences Liquid chromatography Marine Marine Biology Marine ecosystems Mass spectrometry Metabolites Microbial Ecology Microbiology Micromonospora Mollusca Mollusks Nature Conservation Phylogeny Plant Extracts Porifera Pseudonocardia RNA, Ribosomal, 16S - genetics Seawater Secondary Metabolism Shellfish Sipuncula Spain Streptomyces Streptomyces - classification Streptomyces - isolation & purification Water Quality/Water Pollution |
| title | Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T08%3A53%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pharmacological%20Potential%20of%20Phylogenetically%20Diverse%20Actinobacteria%20Isolated%20from%20Deep-Sea%20Coral%20Ecosystems%20of%20the%20Submarine%20Avil%C3%A9s%20Canyon%20in%20the%20Cantabrian%20Sea&rft.jtitle=Microbial%20ecology&rft.au=Sarmiento-Vizca%C3%ADno,%20Aida&rft.date=2017-02-01&rft.volume=73&rft.issue=2&rft.spage=338&rft.epage=352&rft.pages=338-352&rft.issn=0095-3628&rft.eissn=1432-184X&rft_id=info:doi/10.1007/s00248-016-0845-2&rft_dat=%3Cjstor_proqu%3E48723404%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1865254077&rft_id=info:pmid/27614749&rft_jstor_id=48723404&rfr_iscdi=true |