In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model
Aim The aim of this study was to investigate the capacity of adipose‐derived stem cells (ADSC), grown in serum‐free conditions, to regenerate bone around titanium discs with different titanium surfaces. Material and methods Ovine ADSC (oADSC) were isolated from seven sheep and cultured using serum‐f...
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| Veröffentlicht in: | Clinical oral implants research 2017-01, Vol.28 (1), p.64-75 |
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| creator | Godoy Zanicotti, Diogo Coates, Dawn Elizabeth Duncan, Warwick John |
| description | Aim
The aim of this study was to investigate the capacity of adipose‐derived stem cells (ADSC), grown in serum‐free conditions, to regenerate bone around titanium discs with different titanium surfaces.
Material and methods
Ovine ADSC (oADSC) were isolated from seven sheep and cultured using serum‐free and osteogenic conditions. Prior to in vivo testing, the growth and osteogenic behaviour of these cells were analysed in vitro using cell proliferation and extracellular matrix mineralisation assays. The bone regenerative capacity of autologous oADSC was evaluated in vivo on titanium discs in a sheep femur epicondyle model. Machined (MTi) and alumina‐blasted (ABTi) titanium discs were used. Bone regeneration within the defects was evaluated after 1 month using histology and histomorphometry. PKH26 cell‐tracking dye was used to verify the persistence of oADSC in the surgical wound.
Results
oADSC sourced from five of seven sheep differentiated into osteoblast‐like cells. Cellular proliferation was reduced only for osteogenically induced oADSC (oOS‐ADSC) grown on ABTi, compared to non‐induced oADSC grown on ABTi and tissue culture polystyrene (P = 0.03 and 0.02 respectively). There was no significant difference for in vitro mineralisation assays comparing oADSC with oOS‐ADSC, regardless of implant surface type. oADSC labelled with PKH26 were detected 1 month after surgery within the defect. There was no difference in bone regeneration between the bone defects treated with oADSC vs. just blood clot.
Conclusion
After 1‐month healing, the use of autologous oADSC did not improve bone regeneration in defects containing titanium devices with different surfaces. |
| doi_str_mv | 10.1111/clr.12761 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1868303127</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826651520</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3861-c63bcee0a38cd63b4d034764f45b8a30446a1698ed113f51dccb828fd1999ee63</originalsourceid><addsrcrecordid>eNqN0V-LFSEYBnCJoj1tXfQFQuimoNn11dHjXC6H_iwcCKKuxdF3Ti4zetKZsyzd9BH6jH2S3M7WRRAkgiI_HtSHkKfAzqCOczfmM-BrBffIChRjDZMM7pMV65hs1qDghDwq5YoxpjrdPSQnXGkppOQr8vUy0kM4JNqniDTjDiNmO4cUaZ1zmG0My0Q9HoLDQpcS4o4WzMv049v3ISPSXU7XkVof9qlgPfSYwwE9LTNO1OE4llc0VEDLZ8Q9HXBaMp2Sx_ExeTDYseCTu_WUfHrz-uPmXbN9__Zyc7FtnNAKGqdE7xCZFdr5um89E-1atUMre20Fa1tloT4MPYAYJHjnes314KHrOkQlTsmLY-4-py8LltlModzezEZMSzGglRZM1B_8D8qVkiA5q_T5X_QqLTnWhxjoOGsV51JW9fKoXE6lZBzMPofJ5hsDzNyWZ2p55ld51T67S1z6Cf0f-butCs6P4DqMePPvJLPZfjhG_gSTlaVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920462255</pqid></control><display><type>article</type><title>In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Godoy Zanicotti, Diogo ; Coates, Dawn Elizabeth ; Duncan, Warwick John</creator><creatorcontrib>Godoy Zanicotti, Diogo ; Coates, Dawn Elizabeth ; Duncan, Warwick John</creatorcontrib><description>Aim
The aim of this study was to investigate the capacity of adipose‐derived stem cells (ADSC), grown in serum‐free conditions, to regenerate bone around titanium discs with different titanium surfaces.
Material and methods
Ovine ADSC (oADSC) were isolated from seven sheep and cultured using serum‐free and osteogenic conditions. Prior to in vivo testing, the growth and osteogenic behaviour of these cells were analysed in vitro using cell proliferation and extracellular matrix mineralisation assays. The bone regenerative capacity of autologous oADSC was evaluated in vivo on titanium discs in a sheep femur epicondyle model. Machined (MTi) and alumina‐blasted (ABTi) titanium discs were used. Bone regeneration within the defects was evaluated after 1 month using histology and histomorphometry. PKH26 cell‐tracking dye was used to verify the persistence of oADSC in the surgical wound.
Results
oADSC sourced from five of seven sheep differentiated into osteoblast‐like cells. Cellular proliferation was reduced only for osteogenically induced oADSC (oOS‐ADSC) grown on ABTi, compared to non‐induced oADSC grown on ABTi and tissue culture polystyrene (P = 0.03 and 0.02 respectively). There was no significant difference for in vitro mineralisation assays comparing oADSC with oOS‐ADSC, regardless of implant surface type. oADSC labelled with PKH26 were detected 1 month after surgery within the defect. There was no difference in bone regeneration between the bone defects treated with oADSC vs. just blood clot.
Conclusion
After 1‐month healing, the use of autologous oADSC did not improve bone regeneration in defects containing titanium devices with different surfaces.</description><identifier>ISSN: 0905-7161</identifier><identifier>EISSN: 1600-0501</identifier><identifier>DOI: 10.1111/clr.12761</identifier><identifier>PMID: 26853552</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adipose Tissue - cytology ; Aluminum ; Aluminum oxide ; Animals ; Assaying ; Autografts ; Biocompatibility ; biomaterials ; Biomedical materials ; Blood coagulation ; Bone growth ; Bone healing ; Bone histomorphometry ; bone implant interactions ; Bone matrix ; Bone Regeneration ; bone substitutes ; Bone surgery ; Cell culture ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Culture Media, Serum-Free ; Defects ; Dental Implants ; Dentistry ; Devices ; Disks ; Extracellular matrix ; Female ; Femur ; Femur - cytology ; Femur - physiology ; Healing ; Histology ; In vitro methods and tests ; In vivo methods and tests ; Mineralization ; Polystyrene ; Polystyrene resins ; Regeneration ; Regeneration (physiology) ; Sheep ; Stem cells ; Stem Cells - physiology ; Surgical implants ; Tissue culture ; Titanium ; Titanium base alloys ; Wounds</subject><ispartof>Clinical oral implants research, 2017-01, Vol.28 (1), p.64-75</ispartof><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3861-c63bcee0a38cd63b4d034764f45b8a30446a1698ed113f51dccb828fd1999ee63</citedby><cites>FETCH-LOGICAL-c3861-c63bcee0a38cd63b4d034764f45b8a30446a1698ed113f51dccb828fd1999ee63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fclr.12761$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fclr.12761$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26853552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Godoy Zanicotti, Diogo</creatorcontrib><creatorcontrib>Coates, Dawn Elizabeth</creatorcontrib><creatorcontrib>Duncan, Warwick John</creatorcontrib><title>In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model</title><title>Clinical oral implants research</title><addtitle>Clin Oral Implants Res</addtitle><description>Aim
The aim of this study was to investigate the capacity of adipose‐derived stem cells (ADSC), grown in serum‐free conditions, to regenerate bone around titanium discs with different titanium surfaces.
Material and methods
Ovine ADSC (oADSC) were isolated from seven sheep and cultured using serum‐free and osteogenic conditions. Prior to in vivo testing, the growth and osteogenic behaviour of these cells were analysed in vitro using cell proliferation and extracellular matrix mineralisation assays. The bone regenerative capacity of autologous oADSC was evaluated in vivo on titanium discs in a sheep femur epicondyle model. Machined (MTi) and alumina‐blasted (ABTi) titanium discs were used. Bone regeneration within the defects was evaluated after 1 month using histology and histomorphometry. PKH26 cell‐tracking dye was used to verify the persistence of oADSC in the surgical wound.
Results
oADSC sourced from five of seven sheep differentiated into osteoblast‐like cells. Cellular proliferation was reduced only for osteogenically induced oADSC (oOS‐ADSC) grown on ABTi, compared to non‐induced oADSC grown on ABTi and tissue culture polystyrene (P = 0.03 and 0.02 respectively). There was no significant difference for in vitro mineralisation assays comparing oADSC with oOS‐ADSC, regardless of implant surface type. oADSC labelled with PKH26 were detected 1 month after surgery within the defect. There was no difference in bone regeneration between the bone defects treated with oADSC vs. just blood clot.
Conclusion
After 1‐month healing, the use of autologous oADSC did not improve bone regeneration in defects containing titanium devices with different surfaces.</description><subject>Adipose Tissue - cytology</subject><subject>Aluminum</subject><subject>Aluminum oxide</subject><subject>Animals</subject><subject>Assaying</subject><subject>Autografts</subject><subject>Biocompatibility</subject><subject>biomaterials</subject><subject>Biomedical materials</subject><subject>Blood coagulation</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone histomorphometry</subject><subject>bone implant interactions</subject><subject>Bone matrix</subject><subject>Bone Regeneration</subject><subject>bone substitutes</subject><subject>Bone surgery</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Culture Media, Serum-Free</subject><subject>Defects</subject><subject>Dental Implants</subject><subject>Dentistry</subject><subject>Devices</subject><subject>Disks</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>Femur</subject><subject>Femur - cytology</subject><subject>Femur - physiology</subject><subject>Healing</subject><subject>Histology</subject><subject>In vitro methods and tests</subject><subject>In vivo methods and tests</subject><subject>Mineralization</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Regeneration</subject><subject>Regeneration (physiology)</subject><subject>Sheep</subject><subject>Stem cells</subject><subject>Stem Cells - physiology</subject><subject>Surgical implants</subject><subject>Tissue culture</subject><subject>Titanium</subject><subject>Titanium base alloys</subject><subject>Wounds</subject><issn>0905-7161</issn><issn>1600-0501</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0V-LFSEYBnCJoj1tXfQFQuimoNn11dHjXC6H_iwcCKKuxdF3Ti4zetKZsyzd9BH6jH2S3M7WRRAkgiI_HtSHkKfAzqCOczfmM-BrBffIChRjDZMM7pMV65hs1qDghDwq5YoxpjrdPSQnXGkppOQr8vUy0kM4JNqniDTjDiNmO4cUaZ1zmG0My0Q9HoLDQpcS4o4WzMv049v3ISPSXU7XkVof9qlgPfSYwwE9LTNO1OE4llc0VEDLZ8Q9HXBaMp2Sx_ExeTDYseCTu_WUfHrz-uPmXbN9__Zyc7FtnNAKGqdE7xCZFdr5um89E-1atUMre20Fa1tloT4MPYAYJHjnes314KHrOkQlTsmLY-4-py8LltlModzezEZMSzGglRZM1B_8D8qVkiA5q_T5X_QqLTnWhxjoOGsV51JW9fKoXE6lZBzMPofJ5hsDzNyWZ2p55ld51T67S1z6Cf0f-butCs6P4DqMePPvJLPZfjhG_gSTlaVQ</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Godoy Zanicotti, Diogo</creator><creator>Coates, Dawn Elizabeth</creator><creator>Duncan, Warwick John</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model</title><author>Godoy Zanicotti, Diogo ; Coates, Dawn Elizabeth ; Duncan, Warwick John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3861-c63bcee0a38cd63b4d034764f45b8a30446a1698ed113f51dccb828fd1999ee63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose Tissue - cytology</topic><topic>Aluminum</topic><topic>Aluminum oxide</topic><topic>Animals</topic><topic>Assaying</topic><topic>Autografts</topic><topic>Biocompatibility</topic><topic>biomaterials</topic><topic>Biomedical materials</topic><topic>Blood coagulation</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone histomorphometry</topic><topic>bone implant interactions</topic><topic>Bone matrix</topic><topic>Bone Regeneration</topic><topic>bone substitutes</topic><topic>Bone surgery</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Culture Media, Serum-Free</topic><topic>Defects</topic><topic>Dental Implants</topic><topic>Dentistry</topic><topic>Devices</topic><topic>Disks</topic><topic>Extracellular matrix</topic><topic>Female</topic><topic>Femur</topic><topic>Femur - cytology</topic><topic>Femur - physiology</topic><topic>Healing</topic><topic>Histology</topic><topic>In vitro methods and tests</topic><topic>In vivo methods and tests</topic><topic>Mineralization</topic><topic>Polystyrene</topic><topic>Polystyrene resins</topic><topic>Regeneration</topic><topic>Regeneration (physiology)</topic><topic>Sheep</topic><topic>Stem cells</topic><topic>Stem Cells - physiology</topic><topic>Surgical implants</topic><topic>Tissue culture</topic><topic>Titanium</topic><topic>Titanium base alloys</topic><topic>Wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godoy Zanicotti, Diogo</creatorcontrib><creatorcontrib>Coates, Dawn Elizabeth</creatorcontrib><creatorcontrib>Duncan, Warwick John</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral implants research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godoy Zanicotti, Diogo</au><au>Coates, Dawn Elizabeth</au><au>Duncan, Warwick John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model</atitle><jtitle>Clinical oral implants research</jtitle><addtitle>Clin Oral Implants Res</addtitle><date>2017-01</date><risdate>2017</risdate><volume>28</volume><issue>1</issue><spage>64</spage><epage>75</epage><pages>64-75</pages><issn>0905-7161</issn><eissn>1600-0501</eissn><abstract>Aim
The aim of this study was to investigate the capacity of adipose‐derived stem cells (ADSC), grown in serum‐free conditions, to regenerate bone around titanium discs with different titanium surfaces.
Material and methods
Ovine ADSC (oADSC) were isolated from seven sheep and cultured using serum‐free and osteogenic conditions. Prior to in vivo testing, the growth and osteogenic behaviour of these cells were analysed in vitro using cell proliferation and extracellular matrix mineralisation assays. The bone regenerative capacity of autologous oADSC was evaluated in vivo on titanium discs in a sheep femur epicondyle model. Machined (MTi) and alumina‐blasted (ABTi) titanium discs were used. Bone regeneration within the defects was evaluated after 1 month using histology and histomorphometry. PKH26 cell‐tracking dye was used to verify the persistence of oADSC in the surgical wound.
Results
oADSC sourced from five of seven sheep differentiated into osteoblast‐like cells. Cellular proliferation was reduced only for osteogenically induced oADSC (oOS‐ADSC) grown on ABTi, compared to non‐induced oADSC grown on ABTi and tissue culture polystyrene (P = 0.03 and 0.02 respectively). There was no significant difference for in vitro mineralisation assays comparing oADSC with oOS‐ADSC, regardless of implant surface type. oADSC labelled with PKH26 were detected 1 month after surgery within the defect. There was no difference in bone regeneration between the bone defects treated with oADSC vs. just blood clot.
Conclusion
After 1‐month healing, the use of autologous oADSC did not improve bone regeneration in defects containing titanium devices with different surfaces.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>26853552</pmid><doi>10.1111/clr.12761</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0905-7161 |
| ispartof | Clinical oral implants research, 2017-01, Vol.28 (1), p.64-75 |
| issn | 0905-7161 1600-0501 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adipose Tissue - cytology Aluminum Aluminum oxide Animals Assaying Autografts Biocompatibility biomaterials Biomedical materials Blood coagulation Bone growth Bone healing Bone histomorphometry bone implant interactions Bone matrix Bone Regeneration bone substitutes Bone surgery Cell culture Cell Differentiation Cell Proliferation Cells, Cultured Culture Media, Serum-Free Defects Dental Implants Dentistry Devices Disks Extracellular matrix Female Femur Femur - cytology Femur - physiology Healing Histology In vitro methods and tests In vivo methods and tests Mineralization Polystyrene Polystyrene resins Regeneration Regeneration (physiology) Sheep Stem cells Stem Cells - physiology Surgical implants Tissue culture Titanium Titanium base alloys Wounds |
| title | In vivo bone regeneration on titanium devices using serum‐free grown adipose‐derived stem cells, in a sheep femur model |
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