Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis
Estrogen receptors (ERs) are the primary mediators of estrogen signaling, and play crucial roles in the reproduction and development of vertebrates. The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine...
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| Veröffentlicht in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2017-05, Vol.207, p.15-21 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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| creator | Lv, Linlan Dong, Xuexing Lv, Fu Zhao, Weihong Yu, Yebin Yang, Wenping |
| description | Estrogen receptors (ERs) are the primary mediators of estrogen signaling, and play crucial roles in the reproduction and development of vertebrates. The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine residues and the residues around them, which constitute two zinc finger motifs and a P-box, are conserved in both vertebrates and invertebrates. A phylogenetic analysis revealed that paER is an orthologue of ER in the polychaete Platynereis dumerilii. A tissue distribution analysis of paER mRNA showed that it is expressed in various tissues, including the body wall, head, esophageal gland, esophagus, stomach, and most strongly in the intestines. Its expression was also measured in P. aibuhitensis after exposure to 17β-estradiol (E2) for 48h. The paER mRNA levels in the body wall were measured after 6, 12, 24, and 48h in E2-exposed and control animals. However, no significant differences in paER expression were observed between them at any time point. This report describes the first molecular characterization of full-length paER and its tissue-specific expression in P. aibuhitensis. |
| doi_str_mv | 10.1016/j.cbpb.2017.02.001 |
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The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine residues and the residues around them, which constitute two zinc finger motifs and a P-box, are conserved in both vertebrates and invertebrates. A phylogenetic analysis revealed that paER is an orthologue of ER in the polychaete Platynereis dumerilii. A tissue distribution analysis of paER mRNA showed that it is expressed in various tissues, including the body wall, head, esophageal gland, esophagus, stomach, and most strongly in the intestines. Its expression was also measured in P. aibuhitensis after exposure to 17β-estradiol (E2) for 48h. The paER mRNA levels in the body wall were measured after 6, 12, 24, and 48h in E2-exposed and control animals. However, no significant differences in paER expression were observed between them at any time point. This report describes the first molecular characterization of full-length paER and its tissue-specific expression in P. aibuhitensis.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2017.02.001</identifier><identifier>PMID: 28188827</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>17β-Estradiol ; Amino Acid Sequence ; Animals ; Aquatic Organisms ; cDNA cloning ; Cloning, Molecular ; Conserved Sequence ; DNA, Complementary - genetics ; DNA, Complementary - metabolism ; Estradiol - metabolism ; Estradiol - pharmacology ; Estrogen receptor ; Gene Expression ; Organ Specificity ; Perinereis aibuhitensis ; Phylogeny ; Polychaeta - classification ; Polychaeta - drug effects ; Polychaeta - genetics ; Polychaeta - metabolism ; Receptors, Estrogen - genetics ; Receptors, Estrogen - metabolism ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Tissue distribution ; Zinc Fingers</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2017-05, Vol.207, p.15-21</ispartof><rights>2017</rights><rights>Copyright © 2017. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-cde33d7cae48d35b84c50337294740cda2419b369ffcdbbda006b384384220bc3</citedby><cites>FETCH-LOGICAL-c356t-cde33d7cae48d35b84c50337294740cda2419b369ffcdbbda006b384384220bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cbpb.2017.02.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28188827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Linlan</creatorcontrib><creatorcontrib>Dong, Xuexing</creatorcontrib><creatorcontrib>Lv, Fu</creatorcontrib><creatorcontrib>Zhao, Weihong</creatorcontrib><creatorcontrib>Yu, Yebin</creatorcontrib><creatorcontrib>Yang, Wenping</creatorcontrib><title>Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Estrogen receptors (ERs) are the primary mediators of estrogen signaling, and play crucial roles in the reproduction and development of vertebrates. The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine residues and the residues around them, which constitute two zinc finger motifs and a P-box, are conserved in both vertebrates and invertebrates. A phylogenetic analysis revealed that paER is an orthologue of ER in the polychaete Platynereis dumerilii. A tissue distribution analysis of paER mRNA showed that it is expressed in various tissues, including the body wall, head, esophageal gland, esophagus, stomach, and most strongly in the intestines. Its expression was also measured in P. aibuhitensis after exposure to 17β-estradiol (E2) for 48h. The paER mRNA levels in the body wall were measured after 6, 12, 24, and 48h in E2-exposed and control animals. However, no significant differences in paER expression were observed between them at any time point. This report describes the first molecular characterization of full-length paER and its tissue-specific expression in P. aibuhitensis.</description><subject>17β-Estradiol</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Aquatic Organisms</subject><subject>cDNA cloning</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>Estradiol - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen receptor</subject><subject>Gene Expression</subject><subject>Organ Specificity</subject><subject>Perinereis aibuhitensis</subject><subject>Phylogeny</subject><subject>Polychaeta - classification</subject><subject>Polychaeta - drug effects</subject><subject>Polychaeta - genetics</subject><subject>Polychaeta - metabolism</subject><subject>Receptors, Estrogen - genetics</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tissue distribution</subject><subject>Zinc Fingers</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3TAQhUVpaV79A10ULbuxo4dtyZBNCc0DEtJFsxZ6jHN18ZVcSQ6kv7663KTLwIBmhnMOmg-hr5S0lNDhfNtas5iWESpawlpC6Ad0TKUYG0qJ-Fh7Mg5NN_bjETrJeUsIl5TTz-iISSqlZOIYlfs4g11nnbCdY_DhCevgsN3opG2B5P_q4mPAcap7DLmk-AQBJ7CwlJhwHQD7gMsG8E4nX6clzi_VDwXwL9hvEviMtTfrxhcI2ecz9GnSc4Yvr-8perz6-fvyprl7uL69_HHXWN4PpbEOOHfCauik472Rne0J54KNneiIdZp1dDR8GKfJOmOcJmQwXHa1GCPG8lP0_ZC7pPhnrZ9XO58tzLMOENesqBxEz0cuuiplB6lNMecEk1qSrwe9KErUnrbaqj1ttaetCFOVdjV9e81fzQ7cf8sb3iq4OAigXvnsIalsPQQLzleCRbno38v_B4WFku4</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Lv, Linlan</creator><creator>Dong, Xuexing</creator><creator>Lv, Fu</creator><creator>Zhao, Weihong</creator><creator>Yu, Yebin</creator><creator>Yang, Wenping</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis</title><author>Lv, Linlan ; Dong, Xuexing ; Lv, Fu ; Zhao, Weihong ; Yu, Yebin ; Yang, Wenping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-cde33d7cae48d35b84c50337294740cda2419b369ffcdbbda006b384384220bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>17β-Estradiol</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Aquatic Organisms</topic><topic>cDNA cloning</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Complementary - metabolism</topic><topic>Estradiol - metabolism</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen receptor</topic><topic>Gene Expression</topic><topic>Organ Specificity</topic><topic>Perinereis aibuhitensis</topic><topic>Phylogeny</topic><topic>Polychaeta - classification</topic><topic>Polychaeta - drug effects</topic><topic>Polychaeta - genetics</topic><topic>Polychaeta - metabolism</topic><topic>Receptors, Estrogen - genetics</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tissue distribution</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Linlan</creatorcontrib><creatorcontrib>Dong, Xuexing</creatorcontrib><creatorcontrib>Lv, Fu</creatorcontrib><creatorcontrib>Zhao, Weihong</creatorcontrib><creatorcontrib>Yu, Yebin</creatorcontrib><creatorcontrib>Yang, Wenping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Linlan</au><au>Dong, Xuexing</au><au>Lv, Fu</au><au>Zhao, Weihong</au><au>Yu, Yebin</au><au>Yang, Wenping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis</atitle><jtitle>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</jtitle><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><date>2017-05</date><risdate>2017</risdate><volume>207</volume><spage>15</spage><epage>21</epage><pages>15-21</pages><issn>1096-4959</issn><eissn>1879-1107</eissn><abstract>Estrogen receptors (ERs) are the primary mediators of estrogen signaling, and play crucial roles in the reproduction and development of vertebrates. The full-length cDNA of Perinereis aibuhitensis estrogen receptor (paER) was cloned and characterized for the first time. The positions of the cysteine residues and the residues around them, which constitute two zinc finger motifs and a P-box, are conserved in both vertebrates and invertebrates. A phylogenetic analysis revealed that paER is an orthologue of ER in the polychaete Platynereis dumerilii. A tissue distribution analysis of paER mRNA showed that it is expressed in various tissues, including the body wall, head, esophageal gland, esophagus, stomach, and most strongly in the intestines. Its expression was also measured in P. aibuhitensis after exposure to 17β-estradiol (E2) for 48h. The paER mRNA levels in the body wall were measured after 6, 12, 24, and 48h in E2-exposed and control animals. However, no significant differences in paER expression were observed between them at any time point. 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| subjects | 17β-Estradiol Amino Acid Sequence Animals Aquatic Organisms cDNA cloning Cloning, Molecular Conserved Sequence DNA, Complementary - genetics DNA, Complementary - metabolism Estradiol - metabolism Estradiol - pharmacology Estrogen receptor Gene Expression Organ Specificity Perinereis aibuhitensis Phylogeny Polychaeta - classification Polychaeta - drug effects Polychaeta - genetics Polychaeta - metabolism Receptors, Estrogen - genetics Receptors, Estrogen - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Homology, Amino Acid Tissue distribution Zinc Fingers |
| title | Molecular cloning and characterization of an estrogen receptor gene in the marine polychaete Perinereis aibuhitensis |
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