Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins
Protection of radiation-induced DNA damage by metallothionein (MT) has been documented, but there is no detailed information about its efficiency compared to other antioxidants or the effect of metals which bind to MT on the protective effect of MT in radiation-induced DNA damage. In this study, we...
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| Veröffentlicht in: | Toxicology letters 2003-01, Vol.136 (3), p.193-198 |
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| creator | Cai, Lu Cherian, M.George |
| description | Protection of radiation-induced DNA damage by metallothionein (MT) has been documented, but there is no detailed information about its efficiency compared to other antioxidants or the effect of metals which bind to MT on the protective effect of MT in radiation-induced DNA damage. In this study, we used a cell-free system to investigate the effect of MT with other antioxidants, such as albumin and glutathione and we compared the efficiency of MT bound to different metals on radiation-induced DNA damage. DNA damage was measured by loss in ethidium bromide/DNA fluorescence and increased mobility of DNA on gel electrophoresis. Gamma rays at 30 Gy induced significant DNA damage and zinc-MT showed a significant higher protection from radiation-induced DNA damage than both glutathione and albumin. Metallothionein bound to other metals, such as copper and cadmium, also showed protection of radiation-induced DNA damage, but the protective effect by zinc-MT was the highest. These results suggest that MT, in particular bound to zinc, is a high-capacity antioxidant to protect radiation-induced DNA damage. |
| doi_str_mv | 10.1016/S0378-4274(02)00359-4 |
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In this study, we used a cell-free system to investigate the effect of MT with other antioxidants, such as albumin and glutathione and we compared the efficiency of MT bound to different metals on radiation-induced DNA damage. DNA damage was measured by loss in ethidium bromide/DNA fluorescence and increased mobility of DNA on gel electrophoresis. Gamma rays at 30 Gy induced significant DNA damage and zinc-MT showed a significant higher protection from radiation-induced DNA damage than both glutathione and albumin. Metallothionein bound to other metals, such as copper and cadmium, also showed protection of radiation-induced DNA damage, but the protective effect by zinc-MT was the highest. These results suggest that MT, in particular bound to zinc, is a high-capacity antioxidant to protect radiation-induced DNA damage.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/S0378-4274(02)00359-4</identifier><identifier>PMID: 12505272</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Antioxidants - pharmacology ; Biological and medical sciences ; Biological effects of radiation ; Cell-Free System ; DNA - chemistry ; DNA - drug effects ; DNA - radiation effects ; DNA damage ; DNA Damage - drug effects ; DNA Damage - radiation effects ; Dose-Response Relationship, Radiation ; Electrophoresis, Agar Gel ; Fundamental and applied biological sciences. Psychology ; Gamma Rays ; Glutathione ; Glutathione - pharmacology ; Ionizing radiations ; Metallothionein ; Metallothionein - pharmacology ; Metals ; Radiation ; Radiation Effects ; Radiation-Protective Agents ; Tissues, organs and organisms biophysics ; Zinc</subject><ispartof>Toxicology letters, 2003-01, Vol.136 (3), p.193-198</ispartof><rights>2002 Elsevier Science Ireland Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-aea5974fd1b4f538c3e5ce4a85f14737187003d525c6649727e183b2466f3b083</citedby><cites>FETCH-LOGICAL-c488t-aea5974fd1b4f538c3e5ce4a85f14737187003d525c6649727e183b2466f3b083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378427402003594$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14434238$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12505272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Lu</creatorcontrib><creatorcontrib>Cherian, M.George</creatorcontrib><title>Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>Protection of radiation-induced DNA damage by metallothionein (MT) has been documented, but there is no detailed information about its efficiency compared to other antioxidants or the effect of metals which bind to MT on the protective effect of MT in radiation-induced DNA damage. In this study, we used a cell-free system to investigate the effect of MT with other antioxidants, such as albumin and glutathione and we compared the efficiency of MT bound to different metals on radiation-induced DNA damage. DNA damage was measured by loss in ethidium bromide/DNA fluorescence and increased mobility of DNA on gel electrophoresis. Gamma rays at 30 Gy induced significant DNA damage and zinc-MT showed a significant higher protection from radiation-induced DNA damage than both glutathione and albumin. Metallothionein bound to other metals, such as copper and cadmium, also showed protection of radiation-induced DNA damage, but the protective effect by zinc-MT was the highest. These results suggest that MT, in particular bound to zinc, is a high-capacity antioxidant to protect radiation-induced DNA damage.</description><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Cell-Free System</subject><subject>DNA - chemistry</subject><subject>DNA - drug effects</subject><subject>DNA - radiation effects</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>DNA Damage - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Electrophoresis, Agar Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays</subject><subject>Glutathione</subject><subject>Glutathione - pharmacology</subject><subject>Ionizing radiations</subject><subject>Metallothionein</subject><subject>Metallothionein - pharmacology</subject><subject>Metals</subject><subject>Radiation</subject><subject>Radiation Effects</subject><subject>Radiation-Protective Agents</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Zinc</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEURi0EIkvgJ4DcEEExwW97KhSFpxRBATQ0lse-kxjNeBbbEyktvzze7IpINFS3Od99nIvQc0pOKaHqzTfCtekE0-IVYa8J4bLvxAO0oUb3Haeqf4g2f5Ej9KSUX4QQJZR8jI4ok0QyzTboz8-YfDdDddO01Ku4JIgJb_NSwdeCx7zM-N2XMxzc7C4BxxRWDwEPNzi7EF1tATxArZBxvXIJX05rdfs-2KWA_bLdQu7wkrF3YY7r_O-w8hQ9Gt1U4NmhHqMfH95_P__UXXz9-Pn87KLzwpjaOXCy12IMdBCj5MZzkB6EM3KkQnPdDm8WgmTSKyV6zTRQwwcmlBr5QAw_Rif7vu263yuUaudYPEyTS7CsxVKjFOu1bKDcgz4vpWQY7TbH2eUbS4ndybd38u3OrCXM3sm3ouVeHAaswwzhPnWw3YCXB8AV76Yxu-RjueeE4ILx3aZv9xw0HdcRsi0-QmriY25vsWGJ_1nlFrjoon4</recordid><startdate>20030113</startdate><enddate>20030113</enddate><creator>Cai, Lu</creator><creator>Cherian, M.George</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20030113</creationdate><title>Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins</title><author>Cai, Lu ; Cherian, M.George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-aea5974fd1b4f538c3e5ce4a85f14737187003d525c6649727e183b2466f3b083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Cell-Free System</topic><topic>DNA - chemistry</topic><topic>DNA - drug effects</topic><topic>DNA - radiation effects</topic><topic>DNA damage</topic><topic>DNA Damage - drug effects</topic><topic>DNA Damage - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Electrophoresis, Agar Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays</topic><topic>Glutathione</topic><topic>Glutathione - pharmacology</topic><topic>Ionizing radiations</topic><topic>Metallothionein</topic><topic>Metallothionein - pharmacology</topic><topic>Metals</topic><topic>Radiation</topic><topic>Radiation Effects</topic><topic>Radiation-Protective Agents</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Lu</creatorcontrib><creatorcontrib>Cherian, M.George</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Lu</au><au>Cherian, M.George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2003-01-13</date><risdate>2003</risdate><volume>136</volume><issue>3</issue><spage>193</spage><epage>198</epage><pages>193-198</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Protection of radiation-induced DNA damage by metallothionein (MT) has been documented, but there is no detailed information about its efficiency compared to other antioxidants or the effect of metals which bind to MT on the protective effect of MT in radiation-induced DNA damage. In this study, we used a cell-free system to investigate the effect of MT with other antioxidants, such as albumin and glutathione and we compared the efficiency of MT bound to different metals on radiation-induced DNA damage. DNA damage was measured by loss in ethidium bromide/DNA fluorescence and increased mobility of DNA on gel electrophoresis. Gamma rays at 30 Gy induced significant DNA damage and zinc-MT showed a significant higher protection from radiation-induced DNA damage than both glutathione and albumin. Metallothionein bound to other metals, such as copper and cadmium, also showed protection of radiation-induced DNA damage, but the protective effect by zinc-MT was the highest. These results suggest that MT, in particular bound to zinc, is a high-capacity antioxidant to protect radiation-induced DNA damage.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>12505272</pmid><doi>10.1016/S0378-4274(02)00359-4</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 2003-01, Vol.136 (3), p.193-198 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antioxidants - pharmacology Biological and medical sciences Biological effects of radiation Cell-Free System DNA - chemistry DNA - drug effects DNA - radiation effects DNA damage DNA Damage - drug effects DNA Damage - radiation effects Dose-Response Relationship, Radiation Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Gamma Rays Glutathione Glutathione - pharmacology Ionizing radiations Metallothionein Metallothionein - pharmacology Metals Radiation Radiation Effects Radiation-Protective Agents Tissues, organs and organisms biophysics Zinc |
| title | Zinc-metallothionein protects from DNA damage induced by radiation better than glutathione and copper- or cadmium-metallothioneins |
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