The effect of some cryoprotectants on dromedary camel frozen‐thawed semen
Contents The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi cam...
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| Veröffentlicht in: | Reproduction in domestic animals 2017-06, Vol.52 (3), p.522-525 |
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| creator | El‐Badry, DA Mohamed, RH EL‐Metwally, HA Abo Al‐Naga, TR |
| description | Contents
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh‐G), dimethyl formamide (DMF, Sh‐DF), dimethyl sulfoxide (DMSO, Sh‐DS) or ethylene glycol (EG, Sh‐EG), all at 6% final concentration, and the samples were subjected to cryopreservation. The results revealed the superiority of Sh‐DF over Sh‐G and Sh‐DS in terms of post‐thaw motility (55.83 ± 2.20 vs. 47.50 ± 4.33 and 45.00 ± 2.89%, respectively), sperm membrane (49.00 ± 0.58, 39.33 ± 3.33 and 42.67 ± 1.45%, respectively) and acrosomal integrities (53.00 ± 0.58, 57.33 ± 0.88 and 52.33 ± 1.45%, respectively). Sh‐EG group showed the lowest post‐thaw motility, plasma membrane and acrosome integrities (12.50 ± 1.44, 22.67 ± 1.45 and 30.67 ± 1.45, respectively). In conclusion, the protocols of dromedary camel semen cryopreservation could be enhanced using 6% DMF as a cryoprotectant agent. |
| doi_str_mv | 10.1111/rda.12934 |
| format | Article |
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The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh‐G), dimethyl formamide (DMF, Sh‐DF), dimethyl sulfoxide (DMSO, Sh‐DS) or ethylene glycol (EG, Sh‐EG), all at 6% final concentration, and the samples were subjected to cryopreservation. The results revealed the superiority of Sh‐DF over Sh‐G and Sh‐DS in terms of post‐thaw motility (55.83 ± 2.20 vs. 47.50 ± 4.33 and 45.00 ± 2.89%, respectively), sperm membrane (49.00 ± 0.58, 39.33 ± 3.33 and 42.67 ± 1.45%, respectively) and acrosomal integrities (53.00 ± 0.58, 57.33 ± 0.88 and 52.33 ± 1.45%, respectively). Sh‐EG group showed the lowest post‐thaw motility, plasma membrane and acrosome integrities (12.50 ± 1.44, 22.67 ± 1.45 and 30.67 ± 1.45, respectively). In conclusion, the protocols of dromedary camel semen cryopreservation could be enhanced using 6% DMF as a cryoprotectant agent.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12934</identifier><identifier>PMID: 28176384</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acrosome - drug effects ; Animals ; Camels ; Camelus ; Cell Membrane - drug effects ; Cryogenic engineering ; Cryopreservation ; Cryopreservation - veterinary ; cryoprotectant ; Cryoprotectants ; Cryoprotective Agents - pharmacology ; Cryoprotectors ; Dilution ; Dimethyl sulfoxide ; Dimethyl Sulfoxide - pharmacology ; Dimethylformamide ; Dimethylformamide - pharmacology ; DMF ; DMSO ; dromedary camel ; Ethylene ; Ethylene glycol ; Ethylene Glycol - pharmacology ; Fertility ; freezing ; Glycerol ; Glycerol - pharmacology ; Male ; Motility ; Semen ; Semen Preservation - veterinary ; Sperm ; Sperm Motility - drug effects</subject><ispartof>Reproduction in domestic animals, 2017-06, Vol.52 (3), p.522-525</ispartof><rights>2017 Blackwell Verlag GmbH</rights><rights>2017 Blackwell Verlag GmbH.</rights><rights>Copyright © 2017 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-a5de3601e57f85510ae7e2e72ea4e7ffb5d902f1266c8a37df618b72be77a3f83</citedby><cites>FETCH-LOGICAL-c3534-a5de3601e57f85510ae7e2e72ea4e7ffb5d902f1266c8a37df618b72be77a3f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12934$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12934$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28176384$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El‐Badry, DA</creatorcontrib><creatorcontrib>Mohamed, RH</creatorcontrib><creatorcontrib>EL‐Metwally, HA</creatorcontrib><creatorcontrib>Abo Al‐Naga, TR</creatorcontrib><title>The effect of some cryoprotectants on dromedary camel frozen‐thawed semen</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh‐G), dimethyl formamide (DMF, Sh‐DF), dimethyl sulfoxide (DMSO, Sh‐DS) or ethylene glycol (EG, Sh‐EG), all at 6% final concentration, and the samples were subjected to cryopreservation. The results revealed the superiority of Sh‐DF over Sh‐G and Sh‐DS in terms of post‐thaw motility (55.83 ± 2.20 vs. 47.50 ± 4.33 and 45.00 ± 2.89%, respectively), sperm membrane (49.00 ± 0.58, 39.33 ± 3.33 and 42.67 ± 1.45%, respectively) and acrosomal integrities (53.00 ± 0.58, 57.33 ± 0.88 and 52.33 ± 1.45%, respectively). Sh‐EG group showed the lowest post‐thaw motility, plasma membrane and acrosome integrities (12.50 ± 1.44, 22.67 ± 1.45 and 30.67 ± 1.45, respectively). In conclusion, the protocols of dromedary camel semen cryopreservation could be enhanced using 6% DMF as a cryoprotectant agent.</description><subject>Acrosome - drug effects</subject><subject>Animals</subject><subject>Camels</subject><subject>Camelus</subject><subject>Cell Membrane - drug effects</subject><subject>Cryogenic engineering</subject><subject>Cryopreservation</subject><subject>Cryopreservation - veterinary</subject><subject>cryoprotectant</subject><subject>Cryoprotectants</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Cryoprotectors</subject><subject>Dilution</subject><subject>Dimethyl sulfoxide</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Dimethylformamide</subject><subject>Dimethylformamide - pharmacology</subject><subject>DMF</subject><subject>DMSO</subject><subject>dromedary camel</subject><subject>Ethylene</subject><subject>Ethylene glycol</subject><subject>Ethylene Glycol - pharmacology</subject><subject>Fertility</subject><subject>freezing</subject><subject>Glycerol</subject><subject>Glycerol - pharmacology</subject><subject>Male</subject><subject>Motility</subject><subject>Semen</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MtKAzEUBuAgiq3VhS8gATe6mDaXyWWWpV5REKSuh3TmhLbMTGoypdSVj-Az-iSmtroQzCaQfPyc8yN0SkmfxjPwpelTlvF0D3VpyrOECE73UZdkXCZSSd1BRyHMCaFCK3WIOkxTJblOu-hhPAUM1kLRYmdxcDXgwq_dwrs2vpmmDdg1uPTxozR-jQtTQ4Wtd2_QfL5_tFOzghIHqKE5RgfWVAFOdncPvdxcj0d3yePT7f1o-JgUXPA0MaIELgkFoawWghIDChgoBiYFZe1ElBlhljIpC224Kq2keqLYBJQy3GreQxfb3Djk6xJCm9ezUEBVmQbcMuRUS8kykQkW6fkfOndL38TpotoATTMa1eVWFd6F4MHmCz-r47Y5Jfmm4Tw2nH83HO3ZLnE5iZX8yp9KIxhswWpWwfr_pPz5ariN_AKJaoV0</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>El‐Badry, DA</creator><creator>Mohamed, RH</creator><creator>EL‐Metwally, HA</creator><creator>Abo Al‐Naga, TR</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>The effect of some cryoprotectants on dromedary camel frozen‐thawed semen</title><author>El‐Badry, DA ; Mohamed, RH ; EL‐Metwally, HA ; Abo Al‐Naga, TR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-a5de3601e57f85510ae7e2e72ea4e7ffb5d902f1266c8a37df618b72be77a3f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acrosome - drug effects</topic><topic>Animals</topic><topic>Camels</topic><topic>Camelus</topic><topic>Cell Membrane - drug effects</topic><topic>Cryogenic engineering</topic><topic>Cryopreservation</topic><topic>Cryopreservation - veterinary</topic><topic>cryoprotectant</topic><topic>Cryoprotectants</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Cryoprotectors</topic><topic>Dilution</topic><topic>Dimethyl sulfoxide</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>Dimethylformamide</topic><topic>Dimethylformamide - pharmacology</topic><topic>DMF</topic><topic>DMSO</topic><topic>dromedary camel</topic><topic>Ethylene</topic><topic>Ethylene glycol</topic><topic>Ethylene Glycol - pharmacology</topic><topic>Fertility</topic><topic>freezing</topic><topic>Glycerol</topic><topic>Glycerol - pharmacology</topic><topic>Male</topic><topic>Motility</topic><topic>Semen</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El‐Badry, DA</creatorcontrib><creatorcontrib>Mohamed, RH</creatorcontrib><creatorcontrib>EL‐Metwally, HA</creatorcontrib><creatorcontrib>Abo Al‐Naga, TR</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El‐Badry, DA</au><au>Mohamed, RH</au><au>EL‐Metwally, HA</au><au>Abo Al‐Naga, TR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of some cryoprotectants on dromedary camel frozen‐thawed semen</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2017-06</date><risdate>2017</risdate><volume>52</volume><issue>3</issue><spage>522</spage><epage>525</epage><pages>522-525</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The cryopreserved camel semen is often associated with poor quality and fertility. This study aimed to improve the dromedary frozen semen quality by comparing the efficiency of four cryoprotectant agents (CPAs) on sperm freezability. Semen samples were collected from seven male Maghrabi camels, diluted with Shotor diluent supplemented with glycerol (Sh‐G), dimethyl formamide (DMF, Sh‐DF), dimethyl sulfoxide (DMSO, Sh‐DS) or ethylene glycol (EG, Sh‐EG), all at 6% final concentration, and the samples were subjected to cryopreservation. The results revealed the superiority of Sh‐DF over Sh‐G and Sh‐DS in terms of post‐thaw motility (55.83 ± 2.20 vs. 47.50 ± 4.33 and 45.00 ± 2.89%, respectively), sperm membrane (49.00 ± 0.58, 39.33 ± 3.33 and 42.67 ± 1.45%, respectively) and acrosomal integrities (53.00 ± 0.58, 57.33 ± 0.88 and 52.33 ± 1.45%, respectively). Sh‐EG group showed the lowest post‐thaw motility, plasma membrane and acrosome integrities (12.50 ± 1.44, 22.67 ± 1.45 and 30.67 ± 1.45, respectively). In conclusion, the protocols of dromedary camel semen cryopreservation could be enhanced using 6% DMF as a cryoprotectant agent.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>28176384</pmid><doi>10.1111/rda.12934</doi><tpages>4</tpages></addata></record> |
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| subjects | Acrosome - drug effects Animals Camels Camelus Cell Membrane - drug effects Cryogenic engineering Cryopreservation Cryopreservation - veterinary cryoprotectant Cryoprotectants Cryoprotective Agents - pharmacology Cryoprotectors Dilution Dimethyl sulfoxide Dimethyl Sulfoxide - pharmacology Dimethylformamide Dimethylformamide - pharmacology DMF DMSO dromedary camel Ethylene Ethylene glycol Ethylene Glycol - pharmacology Fertility freezing Glycerol Glycerol - pharmacology Male Motility Semen Semen Preservation - veterinary Sperm Sperm Motility - drug effects |
| title | The effect of some cryoprotectants on dromedary camel frozen‐thawed semen |
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