A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product
The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect di...
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| Veröffentlicht in: | Journal of immunological methods 2017-05, Vol.444, p.51-55 |
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| container_title | Journal of immunological methods |
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| creator | Rasmussen, Karina Juhl Skjoedt, Mikkel-Ole Vitved, Lars Skjoedt, Karsten Palarasah, Yaseelan |
| description | The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysiological mechanisms.
We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3.
We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics. |
| doi_str_mv | 10.1016/j.jim.2017.02.002 |
| format | Article |
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We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3.
We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2017.02.002</identifier><identifier>PMID: 28174050</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Binding Sites, Antibody ; C3d ; Clinical diagnostic ; Complement Activation ; Complement C3d - administration & dosage ; Complement C3d - immunology ; Complement C3d - metabolism ; End specificity ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunization ; Injections, Subcutaneous ; Monoclonal antibody ; Protein Binding ; Protein Interaction Domains and Motifs ; Rats, Sprague-Dawley</subject><ispartof>Journal of immunological methods, 2017-05, Vol.444, p.51-55</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-217c42614e69fb4b158b6f13723d0ed5668845f96543aa002a8af389c276bfe93</citedby><cites>FETCH-LOGICAL-c353t-217c42614e69fb4b158b6f13723d0ed5668845f96543aa002a8af389c276bfe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175916302824$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28174050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rasmussen, Karina Juhl</creatorcontrib><creatorcontrib>Skjoedt, Mikkel-Ole</creatorcontrib><creatorcontrib>Vitved, Lars</creatorcontrib><creatorcontrib>Skjoedt, Karsten</creatorcontrib><creatorcontrib>Palarasah, Yaseelan</creatorcontrib><title>A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysiological mechanisms.
We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3.
We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Binding Sites, Antibody</subject><subject>C3d</subject><subject>Clinical diagnostic</subject><subject>Complement Activation</subject><subject>Complement C3d - administration & dosage</subject><subject>Complement C3d - immunology</subject><subject>Complement C3d - metabolism</subject><subject>End specificity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Immunization</subject><subject>Injections, Subcutaneous</subject><subject>Monoclonal antibody</subject><subject>Protein Binding</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Rats, Sprague-Dawley</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv2zAQhImgQe2k_QG5FDz2InVJiQ-hp8DIo0CAXpprCIpawTQk0RWpFP73oWunx54W2P1mFjOE3DAoGTD5bVfu_FhyYKoEXgLwC7JmWvFCNSA-kHXe8IIp0azIVYw7AGAg4SNZcc1UDQLW5OWWTuEVB2qn5LfLaCe6qTo6him4IUz2dGhDd6B_fNrSuEfne-98OtAUaNriX96FcT_giFPKxOAT3c-hW1z6RC57O0T8fJ7X5Pn-7tfmsXj6-fBjc_tUuEpUqeBMuZpLVqNs-rZumdCt7FmleNUBdkJKrWvRN1LUlbU5ltW2r3TjuJJtj011Tb6efPPf3wvGZEYfHQ6DnTAs0TAtJW-4UDqj7IS6OcQ4Y2_2sx_tfDAMzLFWszO5VnOs1QA3-VvWfDnbL-2I3T_Fe48Z-H4CMId89Tib6DxODjs_o0umC_4_9m8X2YeZ</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Rasmussen, Karina Juhl</creator><creator>Skjoedt, Mikkel-Ole</creator><creator>Vitved, Lars</creator><creator>Skjoedt, Karsten</creator><creator>Palarasah, Yaseelan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201705</creationdate><title>A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product</title><author>Rasmussen, Karina Juhl ; Skjoedt, Mikkel-Ole ; Vitved, Lars ; Skjoedt, Karsten ; Palarasah, Yaseelan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-217c42614e69fb4b158b6f13723d0ed5668845f96543aa002a8af389c276bfe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Binding Sites, Antibody</topic><topic>C3d</topic><topic>Clinical diagnostic</topic><topic>Complement Activation</topic><topic>Complement C3d - administration & dosage</topic><topic>Complement C3d - immunology</topic><topic>Complement C3d - metabolism</topic><topic>End specificity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Immunization</topic><topic>Injections, Subcutaneous</topic><topic>Monoclonal antibody</topic><topic>Protein Binding</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rasmussen, Karina Juhl</creatorcontrib><creatorcontrib>Skjoedt, Mikkel-Ole</creatorcontrib><creatorcontrib>Vitved, Lars</creatorcontrib><creatorcontrib>Skjoedt, Karsten</creatorcontrib><creatorcontrib>Palarasah, Yaseelan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rasmussen, Karina Juhl</au><au>Skjoedt, Mikkel-Ole</au><au>Vitved, Lars</au><au>Skjoedt, Karsten</au><au>Palarasah, Yaseelan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2017-05</date><risdate>2017</risdate><volume>444</volume><spage>51</spage><epage>55</epage><pages>51-55</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>The complement component C3 and the cleavage products of C3b/iC3b, C3c and C3d are used as biomarkers in clinical diagnostics. Currently, no specific antibodies are able to differentiate C3d from other fragments, although such a distinction could be very valuable considering that they may reflect different pathophysiological mechanisms.
We have developed a rat antihuman C3d monoclonal antibody with specificity to the end sequence of the N-terminal region of C3d. The antibody can therefore only bind to C3d when it manifests itself as the final end product of cleaved C3.
We believe that this specificity is it first of its kind, and predicts that it can be used as a detection tool in several immunological methods with great value in diagnostics.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28174050</pmid><doi>10.1016/j.jim.2017.02.002</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 2017-05, Vol.444, p.51-55 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibody Specificity Binding Sites, Antibody C3d Clinical diagnostic Complement Activation Complement C3d - administration & dosage Complement C3d - immunology Complement C3d - metabolism End specificity Enzyme-Linked Immunosorbent Assay Humans Immunization Injections, Subcutaneous Monoclonal antibody Protein Binding Protein Interaction Domains and Motifs Rats, Sprague-Dawley |
| title | A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product |
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