Tn5 Transposase Active Site Mutations Suggest Position of Donor Backbone DNA in Synaptic Complex

Tn5 transposase (Tnp), a 53.3-kDa protein, enables the movement of transposon Tn5 by a conservative mechanism. Within the context of a protein and DNA synaptic complex, a single Tnp molecule catalyzes four sequential DNA breaking and joining reactions at the end of a single transposon. The three ami...

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Veröffentlicht in:	The Journal of biological chemistry 2003-01, Vol.278 (3), p.1904-1909
Hauptverfasser:	Peterson, Gregory, Reznikoff, William
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA Primers DNA, Bacterial - metabolism Electrophoresis, Polyacrylamide Gel Models, Molecular Mutagenesis, Site-Directed Substrate Specificity Synapses - enzymology Synapses - metabolism Transposases - chemistry Transposases - genetics Transposases - metabolism
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