Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth
Aims To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cell...
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| Veröffentlicht in: | International endodontic journal 2017-12, Vol.50 (S2), p.e19-e30 |
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| creator | Collado‐González, M. García‐Bernal, D. Oñate‐Sánchez, R. E. Ortolani‐Seltenerich, P. S. Álvarez‐Muro, T. Lozano, A. Forner, L. Llena, C. Moraleda, J. M. Rodríguez‐Lozano, F. J. |
| description | Aims
To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs).
Methodology
SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound‐healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05).
Results
Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P |
| doi_str_mv | 10.1111/iej.12751 |
| format | Article |
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To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs).
Methodology
SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound‐healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05).
Results
Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P < 0.01) and was also significantly higher than using MTA Angelus from 48 h of incubation (P < 0.01). However, Theracal LC and IRM were associated with low rates of cell viability (P < 0.001). Similar results were obtained in an apoptosis assay. In addition, SHEDs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. SEM studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and MTA Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7 days of culture (P < 0.01).
Conclusions
Biodentine exhibited better cytocompatibility and bioactivity than MTA Angelus, Theracal LC and IRM.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.12751</identifier><identifier>PMID: 28169432</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Aluminum Compounds - pharmacology ; Aluminum Compounds - toxicity ; Apoptosis ; Apoptosis - drug effects ; Biological activity ; Calcification ; Calcium Compounds - pharmacology ; Calcium Compounds - toxicity ; Cell culture ; Cell migration ; Cell Movement - drug effects ; Cell spreading ; Cell Survival - drug effects ; Cells, Cultured ; Cytology ; Cytotoxicity ; Dentistry ; Drug Combinations ; Endodontics ; Flow Cytometry ; Humans ; In Vitro Techniques ; Materials Testing ; Mesenchyme ; Methylmethacrylates - pharmacology ; Methylmethacrylates - toxicity ; Microscopy, Electron, Scanning ; mineral trioxide aggregate ; Mitochondria ; Oxides - pharmacology ; Oxides - toxicity ; Phenotype ; Pulp Capping and Pulpectomy Agents - pharmacology ; Pulp Capping and Pulpectomy Agents - toxicity ; Pulpotomy ; pulpotomy materials ; Scanning electron microscopy ; Silicates - pharmacology ; Silicates - toxicity ; Statistical analysis ; Stem cells ; Stem Cells - drug effects ; Teeth ; Time Factors ; Tooth, Deciduous ; Wound healing ; Zinc Oxide-Eugenol Cement - pharmacology ; Zinc Oxide-Eugenol Cement - toxicity</subject><ispartof>International endodontic journal, 2017-12, Vol.50 (S2), p.e19-e30</ispartof><rights>2017 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2017 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2017 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4541-d97a82d773daeb1cfa26973918613746316f6e8f624f2448ba4e1b5f1caaecaf3</citedby><cites>FETCH-LOGICAL-c4541-d97a82d773daeb1cfa26973918613746316f6e8f624f2448ba4e1b5f1caaecaf3</cites><orcidid>0000-0002-0623-740X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.12751$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.12751$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28169432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Collado‐González, M.</creatorcontrib><creatorcontrib>García‐Bernal, D.</creatorcontrib><creatorcontrib>Oñate‐Sánchez, R. E.</creatorcontrib><creatorcontrib>Ortolani‐Seltenerich, P. S.</creatorcontrib><creatorcontrib>Álvarez‐Muro, T.</creatorcontrib><creatorcontrib>Lozano, A.</creatorcontrib><creatorcontrib>Forner, L.</creatorcontrib><creatorcontrib>Llena, C.</creatorcontrib><creatorcontrib>Moraleda, J. M.</creatorcontrib><creatorcontrib>Rodríguez‐Lozano, F. J.</creatorcontrib><title>Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aims
To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs).
Methodology
SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound‐healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05).
Results
Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P < 0.01) and was also significantly higher than using MTA Angelus from 48 h of incubation (P < 0.01). However, Theracal LC and IRM were associated with low rates of cell viability (P < 0.001). Similar results were obtained in an apoptosis assay. In addition, SHEDs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. SEM studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and MTA Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7 days of culture (P < 0.01).
Conclusions
Biodentine exhibited better cytocompatibility and bioactivity than MTA Angelus, Theracal LC and IRM.</description><subject>Aluminum Compounds - pharmacology</subject><subject>Aluminum Compounds - toxicity</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological activity</subject><subject>Calcification</subject><subject>Calcium Compounds - pharmacology</subject><subject>Calcium Compounds - toxicity</subject><subject>Cell culture</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cell spreading</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cytology</subject><subject>Cytotoxicity</subject><subject>Dentistry</subject><subject>Drug Combinations</subject><subject>Endodontics</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Materials Testing</subject><subject>Mesenchyme</subject><subject>Methylmethacrylates - pharmacology</subject><subject>Methylmethacrylates - toxicity</subject><subject>Microscopy, Electron, Scanning</subject><subject>mineral trioxide aggregate</subject><subject>Mitochondria</subject><subject>Oxides - pharmacology</subject><subject>Oxides - toxicity</subject><subject>Phenotype</subject><subject>Pulp Capping and Pulpectomy Agents - pharmacology</subject><subject>Pulp Capping and Pulpectomy Agents - toxicity</subject><subject>Pulpotomy</subject><subject>pulpotomy materials</subject><subject>Scanning electron microscopy</subject><subject>Silicates - pharmacology</subject><subject>Silicates - toxicity</subject><subject>Statistical analysis</subject><subject>Stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Teeth</subject><subject>Time Factors</subject><subject>Tooth, Deciduous</subject><subject>Wound healing</subject><subject>Zinc Oxide-Eugenol Cement - pharmacology</subject><subject>Zinc Oxide-Eugenol Cement - toxicity</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EokvhwAsgS1zgkDZjO45zRKsCrSpxKWfLScaqV3G82E5p3h5vd-GAVF-skb75NDM_Ie-hvoDyLh3uLoC1DbwgG-CyqVjTwUuyqUHwiinVnJE3Ke3qum5qDq_JGVMgO8HZhvjtmkMOj25weaVmHmnvghmyezjUwdIHE11YEt0v076AfqXeZIzOTImGmaaMng44lcrG4On94s1M8dGGyRVupPvovIkrzYj5_i15ZUsjvjv95-Tn16u77ffq9se36-2X22oQjYBq7Fqj2Ni2fDTYw2ANk13LO1ASeCskB2klKiuZsEwI1RuB0DcWBmNwMJafk09H7z6GXwumrL1LhynNjGUZXURNOYFUUNCP_6G7sMS5TKehk7JVnWgP1OcjNcSQUkSrT3tpqPUhA10y0E8ZFPbDybj0Hsd_5N-jF-DyCPx2E67Pm_T11c1R-QdYJpIK</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Collado‐González, M.</creator><creator>García‐Bernal, D.</creator><creator>Oñate‐Sánchez, R. E.</creator><creator>Ortolani‐Seltenerich, P. S.</creator><creator>Álvarez‐Muro, T.</creator><creator>Lozano, A.</creator><creator>Forner, L.</creator><creator>Llena, C.</creator><creator>Moraleda, J. M.</creator><creator>Rodríguez‐Lozano, F. J.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0623-740X</orcidid></search><sort><creationdate>201712</creationdate><title>Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth</title><author>Collado‐González, M. ; García‐Bernal, D. ; Oñate‐Sánchez, R. E. ; Ortolani‐Seltenerich, P. S. ; Álvarez‐Muro, T. ; Lozano, A. ; Forner, L. ; Llena, C. ; Moraleda, J. M. ; Rodríguez‐Lozano, F. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4541-d97a82d773daeb1cfa26973918613746316f6e8f624f2448ba4e1b5f1caaecaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aluminum Compounds - pharmacology</topic><topic>Aluminum Compounds - toxicity</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological activity</topic><topic>Calcification</topic><topic>Calcium Compounds - pharmacology</topic><topic>Calcium Compounds - toxicity</topic><topic>Cell culture</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Cell spreading</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cytology</topic><topic>Cytotoxicity</topic><topic>Dentistry</topic><topic>Drug Combinations</topic><topic>Endodontics</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Materials Testing</topic><topic>Mesenchyme</topic><topic>Methylmethacrylates - pharmacology</topic><topic>Methylmethacrylates - toxicity</topic><topic>Microscopy, Electron, Scanning</topic><topic>mineral trioxide aggregate</topic><topic>Mitochondria</topic><topic>Oxides - pharmacology</topic><topic>Oxides - toxicity</topic><topic>Phenotype</topic><topic>Pulp Capping and Pulpectomy Agents - pharmacology</topic><topic>Pulp Capping and Pulpectomy Agents - toxicity</topic><topic>Pulpotomy</topic><topic>pulpotomy materials</topic><topic>Scanning electron microscopy</topic><topic>Silicates - pharmacology</topic><topic>Silicates - toxicity</topic><topic>Statistical analysis</topic><topic>Stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Teeth</topic><topic>Time Factors</topic><topic>Tooth, Deciduous</topic><topic>Wound healing</topic><topic>Zinc Oxide-Eugenol Cement - pharmacology</topic><topic>Zinc Oxide-Eugenol Cement - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collado‐González, M.</creatorcontrib><creatorcontrib>García‐Bernal, D.</creatorcontrib><creatorcontrib>Oñate‐Sánchez, R. E.</creatorcontrib><creatorcontrib>Ortolani‐Seltenerich, P. S.</creatorcontrib><creatorcontrib>Álvarez‐Muro, T.</creatorcontrib><creatorcontrib>Lozano, A.</creatorcontrib><creatorcontrib>Forner, L.</creatorcontrib><creatorcontrib>Llena, C.</creatorcontrib><creatorcontrib>Moraleda, J. M.</creatorcontrib><creatorcontrib>Rodríguez‐Lozano, F. J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collado‐González, M.</au><au>García‐Bernal, D.</au><au>Oñate‐Sánchez, R. E.</au><au>Ortolani‐Seltenerich, P. S.</au><au>Álvarez‐Muro, T.</au><au>Lozano, A.</au><au>Forner, L.</au><au>Llena, C.</au><au>Moraleda, J. M.</au><au>Rodríguez‐Lozano, F. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2017-12</date><risdate>2017</risdate><volume>50</volume><issue>S2</issue><spage>e19</spage><epage>e30</epage><pages>e19-e30</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aims
To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR, Brazil), Theracal LC (Bisco Inc., Schamburg, IL, USA) and IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHEDs).
Methodology
SHEDs were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72 h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in vitro scratch wound‐healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHEDs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post‐test (α = 0.05).
Results
Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P < 0.01) and was also significantly higher than using MTA Angelus from 48 h of incubation (P < 0.01). However, Theracal LC and IRM were associated with low rates of cell viability (P < 0.001). Similar results were obtained in an apoptosis assay. In addition, SHEDs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. SEM studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and MTA Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7 days of culture (P < 0.01).
Conclusions
Biodentine exhibited better cytocompatibility and bioactivity than MTA Angelus, Theracal LC and IRM.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>28169432</pmid><doi>10.1111/iej.12751</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0623-740X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International endodontic journal, 2017-12, Vol.50 (S2), p.e19-e30 |
| issn | 0143-2885 1365-2591 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aluminum Compounds - pharmacology Aluminum Compounds - toxicity Apoptosis Apoptosis - drug effects Biological activity Calcification Calcium Compounds - pharmacology Calcium Compounds - toxicity Cell culture Cell migration Cell Movement - drug effects Cell spreading Cell Survival - drug effects Cells, Cultured Cytology Cytotoxicity Dentistry Drug Combinations Endodontics Flow Cytometry Humans In Vitro Techniques Materials Testing Mesenchyme Methylmethacrylates - pharmacology Methylmethacrylates - toxicity Microscopy, Electron, Scanning mineral trioxide aggregate Mitochondria Oxides - pharmacology Oxides - toxicity Phenotype Pulp Capping and Pulpectomy Agents - pharmacology Pulp Capping and Pulpectomy Agents - toxicity Pulpotomy pulpotomy materials Scanning electron microscopy Silicates - pharmacology Silicates - toxicity Statistical analysis Stem cells Stem Cells - drug effects Teeth Time Factors Tooth, Deciduous Wound healing Zinc Oxide-Eugenol Cement - pharmacology Zinc Oxide-Eugenol Cement - toxicity |
| title | Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth |
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