Live-cell imaging of Salmonella Typhimurium interaction with zebrafish larvae after injection and immersion delivery methods

The zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. Here, we compare the in vivo immune response induced by GFP-tagged Salmonella Typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. Our novel infection...

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Veröffentlicht in:	Journal of microbiological methods 2017-04, Vol.135, p.20-25
Hauptverfasser:	Varas, Macarena, Fariña, Alonso, Díaz-Pascual, Francisco, Ortíz-Severín, Javiera, Marcoleta, Andrés E., Allende, Miguel L., Santiviago, Carlos A., Chávez, Francisco P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified - immunology Animals, Genetically Modified - microbiology Danio rerio Disease Models, Animal Host-pathogen interaction Host-Pathogen Interactions - immunology Immersion Immune response Immunity, Innate - immunology Infection Larva - immunology Larva - microbiology Live-cell imaging Microinjections - methods Microinjections - veterinary Neutrophils - immunology Salmonella Salmonella Infections - diagnostic imaging Salmonella Infections - immunology Salmonella Infections - microbiology Salmonella typhimurium - pathogenicity Zebrafish Zebrafish - immunology Zebrafish - microbiology
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