Evaluation of DNA damage and DNA repair capacity in occupationally lead-exposed workers
Occupational lead (Pb) exposure remains a significant concern for workers in Turkey. Health hazards of Pb exposure have been investigated in various test systems, but results regarding its potential genotoxic effects on exposed populations are contradictory. In this study, a control group and an exp...
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| Veröffentlicht in: | Toxicology and industrial health 2016-11, Vol.32 (11), p.1859-1865 |
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| creator | Jannuzzi, Ayse Tarbin Alpertunga, Buket |
| description | Occupational lead (Pb) exposure remains a significant concern for workers in Turkey. Health hazards of Pb exposure have been investigated in various test systems, but results regarding its potential genotoxic effects on exposed populations are contradictory. In this study, a control group and an exposed group were studied, each consisting of 25 male subjects. Blood lead levels (BLLs) were estimated by graphite furnace atomic absorption spectrometry. Genotoxic effects of Pb exposure were studied in leukocytes by comet and challenge assays. The effect of Pb exposure to DNA repair capacity was evaluated following in vitro hydrogen peroxide exposure. Pb-exposed workers had significantly higher BLLs than the control group (p < 0.01). DNA damage in exposed workers had a significantly higher percentage of DNA in tail than the control group (p < 0.05). In the challenge assay, it was found that the mean DNA% repair capacity was significantly decreased in Pb-exposed workers (p < 0.01). The results indicated that occupational Pb exposure is associated with DNA damage and causes decrease in DNA% repair capacity, indicating a potential health concern for occupationally Pb-exposed populations. |
| doi_str_mv | 10.1177/0748233715590919 |
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Health hazards of Pb exposure have been investigated in various test systems, but results regarding its potential genotoxic effects on exposed populations are contradictory. In this study, a control group and an exposed group were studied, each consisting of 25 male subjects. Blood lead levels (BLLs) were estimated by graphite furnace atomic absorption spectrometry. Genotoxic effects of Pb exposure were studied in leukocytes by comet and challenge assays. The effect of Pb exposure to DNA repair capacity was evaluated following in vitro hydrogen peroxide exposure. Pb-exposed workers had significantly higher BLLs than the control group (p < 0.01). DNA damage in exposed workers had a significantly higher percentage of DNA in tail than the control group (p < 0.05). In the challenge assay, it was found that the mean DNA% repair capacity was significantly decreased in Pb-exposed workers (p < 0.01). 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Health hazards of Pb exposure have been investigated in various test systems, but results regarding its potential genotoxic effects on exposed populations are contradictory. In this study, a control group and an exposed group were studied, each consisting of 25 male subjects. Blood lead levels (BLLs) were estimated by graphite furnace atomic absorption spectrometry. Genotoxic effects of Pb exposure were studied in leukocytes by comet and challenge assays. The effect of Pb exposure to DNA repair capacity was evaluated following in vitro hydrogen peroxide exposure. Pb-exposed workers had significantly higher BLLs than the control group (p < 0.01). DNA damage in exposed workers had a significantly higher percentage of DNA in tail than the control group (p < 0.05). In the challenge assay, it was found that the mean DNA% repair capacity was significantly decreased in Pb-exposed workers (p < 0.01). The results indicated that occupational Pb exposure is associated with DNA damage and causes decrease in DNA% repair capacity, indicating a potential health concern for occupationally Pb-exposed populations.</description><subject>Atomic absorption spectroscopy</subject><subject>Blood levels</subject><subject>Damage</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA repair</subject><subject>Exposure</subject><subject>Health</subject><subject>Health hazards</subject><subject>Hydrogen peroxide</subject><subject>Lead</subject><subject>Lead (metal)</subject><subject>Lead poisoning</subject><subject>Occupational</subject><subject>Occupational exposure</subject><subject>Occupational hazards</subject><subject>Populations</subject><subject>Repair</subject><subject>Spectral analysis</subject><subject>Spectrometry</subject><issn>0748-2337</issn><issn>1477-0393</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqN0c1LG0EYBvChWDS1vXuSBS9etn3ne-cYYqyCtBelx-XNfJTVTXadydrmv--ssSIBIadheH_vMzAPIScUvlKq9TfQomKcayqlAUPNBzKhQusSuOEHZDKOy3F-RD6ldA8ASkl2SI6YoiJzNiG_5k_YDrhuulXRheLix7RwuMTfvsCVe75G32MTC4s92ma9KZoMrR365x1s203RenSl_9t3ybviTxcffEyfyceAbfJfXs5jcnc5v51dlTc_v1_PpjelFZyuS2EQKrbwmlZWOmckdT4Eh9RoTislFAsmcCOCkhDQOoFW82pBBVSSO-75MTnf5vaxexx8WtfLJlnftrjy3ZDqMUTqHA97UKEElQrUHpQpxSSAzvRsh953Q8wfMyrOQFSqMlnBVtnYpRR9qPvYLDFuagr1WGW9W2VeOX0JHhZL714X_neXQbkFKdf15tX3Av8BGZmjQg</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Jannuzzi, Ayse Tarbin</creator><creator>Alpertunga, Buket</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T2</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><scope>7TM</scope><scope>7U2</scope></search><sort><creationdate>201611</creationdate><title>Evaluation of DNA damage and DNA repair capacity in occupationally lead-exposed workers</title><author>Jannuzzi, Ayse Tarbin ; 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Health hazards of Pb exposure have been investigated in various test systems, but results regarding its potential genotoxic effects on exposed populations are contradictory. In this study, a control group and an exposed group were studied, each consisting of 25 male subjects. Blood lead levels (BLLs) were estimated by graphite furnace atomic absorption spectrometry. Genotoxic effects of Pb exposure were studied in leukocytes by comet and challenge assays. The effect of Pb exposure to DNA repair capacity was evaluated following in vitro hydrogen peroxide exposure. Pb-exposed workers had significantly higher BLLs than the control group (p < 0.01). DNA damage in exposed workers had a significantly higher percentage of DNA in tail than the control group (p < 0.05). In the challenge assay, it was found that the mean DNA% repair capacity was significantly decreased in Pb-exposed workers (p < 0.01). 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| source | SAGE Complete A-Z List |
| subjects | Atomic absorption spectroscopy Blood levels Damage Deoxyribonucleic acid DNA DNA damage DNA repair Exposure Health Health hazards Hydrogen peroxide Lead Lead (metal) Lead poisoning Occupational Occupational exposure Occupational hazards Populations Repair Spectral analysis Spectrometry |
| title | Evaluation of DNA damage and DNA repair capacity in occupationally lead-exposed workers |
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