Isolated effect of material stiffness on valvular interstitial cell differentiation
Previous methods for investigating material stiffness on cell behavior have focused on the use of substrates with limited ranges of stiffness and/or fluctuating surface chemistries. Using the co‐polymer system of n‐octyl methacrylate crosslinked with diethylene glycol dimethacrylate (DEGDMA/nOM), we...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2017-01, Vol.105 (1), p.51-61 |
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| container_title | Journal of biomedical materials research. Part A |
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| creator | Coombs, Kent E. Leonard, Alexander T. Rush, Matthew N. Santistevan, David A. Hedberg‐Dirk, Elizabeth L. |
| description | Previous methods for investigating material stiffness on cell behavior have focused on the use of substrates with limited ranges of stiffness and/or fluctuating surface chemistries. Using the co‐polymer system of n‐octyl methacrylate crosslinked with diethylene glycol dimethacrylate (DEGDMA/nOM), we developed a new cell culture platform to analyze the isolated effects of stiffness independent from changes in surface chemistry. Materials ranging from 25 kPa to 4,700 kPa were fabricated. Surface analysis including goiniometry and X‐ray photoelectron spectroscopy (XPS) confirmed consistent surface chemistry across all formulations examined. The mechanosensitive cell type valvular interstitial cell (VIC) was cultured DEGDMA/nOM substrates of differing stiffness. Results indicate that order of magnitude changes in stiffness do not increase gene expression of VIC alpha‐smooth muscle actin (αSMA). However, structural organization of αSMA is altered on stiffer substrates, corresponding with the appearance of the osteoblastic marker osteocalcin and nodule formation. This research presents the co‐polymer DEGDMA/nOM as ideal substrate to investigate the influence of stiffness on VIC differentiation without the confounding effects of changing material surface chemistry. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 51–61, 2017. |
| doi_str_mv | 10.1002/jbm.a.35864 |
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Using the co‐polymer system of n‐octyl methacrylate crosslinked with diethylene glycol dimethacrylate (DEGDMA/nOM), we developed a new cell culture platform to analyze the isolated effects of stiffness independent from changes in surface chemistry. Materials ranging from 25 kPa to 4,700 kPa were fabricated. Surface analysis including goiniometry and X‐ray photoelectron spectroscopy (XPS) confirmed consistent surface chemistry across all formulations examined. The mechanosensitive cell type valvular interstitial cell (VIC) was cultured DEGDMA/nOM substrates of differing stiffness. Results indicate that order of magnitude changes in stiffness do not increase gene expression of VIC alpha‐smooth muscle actin (αSMA). However, structural organization of αSMA is altered on stiffer substrates, corresponding with the appearance of the osteoblastic marker osteocalcin and nodule formation. This research presents the co‐polymer DEGDMA/nOM as ideal substrate to investigate the influence of stiffness on VIC differentiation without the confounding effects of changing material surface chemistry. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 51–61, 2017.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.35864</identifier><identifier>PMID: 27513612</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Antigens, Differentiation - biosynthesis ; Biomedical materials ; Cell Differentiation ; Cells, Cultured ; Crosslinking ; Differentiation ; extracellular matrix ; Gene expression ; Heart Valves - cytology ; Heart Valves - metabolism ; Interstitials ; mechanosensing ; Methacrylates - chemistry ; microenvironment ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Stiffness ; Substrates ; Surface chemistry ; Swine ; valvular interstitial cell</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Previous methods for investigating material stiffness on cell behavior have focused on the use of substrates with limited ranges of stiffness and/or fluctuating surface chemistries. Using the co‐polymer system of n‐octyl methacrylate crosslinked with diethylene glycol dimethacrylate (DEGDMA/nOM), we developed a new cell culture platform to analyze the isolated effects of stiffness independent from changes in surface chemistry. Materials ranging from 25 kPa to 4,700 kPa were fabricated. Surface analysis including goiniometry and X‐ray photoelectron spectroscopy (XPS) confirmed consistent surface chemistry across all formulations examined. The mechanosensitive cell type valvular interstitial cell (VIC) was cultured DEGDMA/nOM substrates of differing stiffness. Results indicate that order of magnitude changes in stiffness do not increase gene expression of VIC alpha‐smooth muscle actin (αSMA). However, structural organization of αSMA is altered on stiffer substrates, corresponding with the appearance of the osteoblastic marker osteocalcin and nodule formation. This research presents the co‐polymer DEGDMA/nOM as ideal substrate to investigate the influence of stiffness on VIC differentiation without the confounding effects of changing material surface chemistry. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 51–61, 2017.</description><subject>Animals</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Biomedical materials</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Crosslinking</subject><subject>Differentiation</subject><subject>extracellular matrix</subject><subject>Gene expression</subject><subject>Heart Valves - cytology</subject><subject>Heart Valves - metabolism</subject><subject>Interstitials</subject><subject>mechanosensing</subject><subject>Methacrylates - chemistry</subject><subject>microenvironment</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Stiffness</subject><subject>Substrates</subject><subject>Surface chemistry</subject><subject>Swine</subject><subject>valvular interstitial cell</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0ctLwzAABvAgipvTk3cpeBGkM-8mRx0-JhMP6jmkbQIdfcykney_N12nBw-yU14_PpJ8AJwjOEUQ4ptlWk31lDDB6QEYI8ZwTCVnh_2cyphgyUfgxPtlwBwyfAxGOGGIcITH4G3um1K3Jo-MtSZro8ZGVVi7QpeRbwtra-N91NTRWpfrrtQuKupwHI7anmSmLKM8MONMHXbaoqlPwZHVpTdnu3ECPh7u32dP8eL1cT67XcQZ5YTG0mCY8iynjDGdpAQlwpIUQoKsxlpYyBm1idTWCpFm2GZUG6s5yiTBmmNLJuBqyF255rMzvlVV4fsL6do0nVco_AjjNGFkD8pgIqCU-1BKCYNcJIFe_qHLpnN1ePNWSZlIJoK6HlTmGu-dsWrlikq7jUJQ9Q2q0KDSattg0Be7zC6tTP5rfyoLAA_gqyjN5r8s9Xz3cjukfgMF0qYU</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Coombs, Kent E.</creator><creator>Leonard, Alexander T.</creator><creator>Rush, Matthew N.</creator><creator>Santistevan, David A.</creator><creator>Hedberg‐Dirk, Elizabeth L.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Isolated effect of material stiffness on valvular interstitial cell differentiation</title><author>Coombs, Kent E. ; Leonard, Alexander T. ; Rush, Matthew N. ; Santistevan, David A. ; Hedberg‐Dirk, Elizabeth L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4634-9e20b6cd4555a7b3178f3b0031fa2a8f0654f79aff88bc2fc4aefa61c932a62f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antigens, Differentiation - biosynthesis</topic><topic>Biomedical materials</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Crosslinking</topic><topic>Differentiation</topic><topic>extracellular matrix</topic><topic>Gene expression</topic><topic>Heart Valves - cytology</topic><topic>Heart Valves - metabolism</topic><topic>Interstitials</topic><topic>mechanosensing</topic><topic>Methacrylates - chemistry</topic><topic>microenvironment</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Stiffness</topic><topic>Substrates</topic><topic>Surface chemistry</topic><topic>Swine</topic><topic>valvular interstitial cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coombs, Kent E.</creatorcontrib><creatorcontrib>Leonard, Alexander T.</creatorcontrib><creatorcontrib>Rush, Matthew N.</creatorcontrib><creatorcontrib>Santistevan, David A.</creatorcontrib><creatorcontrib>Hedberg‐Dirk, Elizabeth L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. 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Using the co‐polymer system of n‐octyl methacrylate crosslinked with diethylene glycol dimethacrylate (DEGDMA/nOM), we developed a new cell culture platform to analyze the isolated effects of stiffness independent from changes in surface chemistry. Materials ranging from 25 kPa to 4,700 kPa were fabricated. Surface analysis including goiniometry and X‐ray photoelectron spectroscopy (XPS) confirmed consistent surface chemistry across all formulations examined. The mechanosensitive cell type valvular interstitial cell (VIC) was cultured DEGDMA/nOM substrates of differing stiffness. Results indicate that order of magnitude changes in stiffness do not increase gene expression of VIC alpha‐smooth muscle actin (αSMA). However, structural organization of αSMA is altered on stiffer substrates, corresponding with the appearance of the osteoblastic marker osteocalcin and nodule formation. 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| subjects | Animals Antigens, Differentiation - biosynthesis Biomedical materials Cell Differentiation Cells, Cultured Crosslinking Differentiation extracellular matrix Gene expression Heart Valves - cytology Heart Valves - metabolism Interstitials mechanosensing Methacrylates - chemistry microenvironment Osteoblasts - cytology Osteoblasts - metabolism Stiffness Substrates Surface chemistry Swine valvular interstitial cell |
| title | Isolated effect of material stiffness on valvular interstitial cell differentiation |
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