Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry
Dopamine (DA) modulates central neuronal activity through both phasic (second to second) and tonic (minutes to hours) terminal release. Conventional fast-scan cyclic voltammetry (FSCV), in combination with carbon fiber microelectrodes, has been used to measure phasic DA release in vivo by adopting a...
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Veröffentlicht in: | Analytical chemistry (Washington) 2016-11, Vol.88 (22), p.10962-10970 |
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creator | Oh, Yoonbae Park, Cheonho Kim, Do Hyoung Shin, Hojin Kang, Yu Min DeWaele, Mark Lee, Jeyeon Min, Hoon-Ki Blaha, Charles D Bennet, Kevin E Kim, In Young Lee, Kendall H Jang, Dong Pyo |
description | Dopamine (DA) modulates central neuronal activity through both phasic (second to second) and tonic (minutes to hours) terminal release. Conventional fast-scan cyclic voltammetry (FSCV), in combination with carbon fiber microelectrodes, has been used to measure phasic DA release in vivo by adopting a background subtraction procedure to remove background capacitive currents. However, measuring tonic changes in DA concentrations using conventional FSCV has been difficult because background capacitive currents are inherently unstable over long recording periods. To measure tonic changes in DA concentrations over several hours, we applied a novel charge-balancing multiple waveform FSCV (CBM-FSCV), combined with a dual background subtraction technique, to minimize temporal variations in background capacitive currents. Using this method, in vitro, charge variations from a reference time point were nearly zero for 48 h, whereas with conventional background subtraction, charge variations progressively increased. CBM-FSCV also demonstrated a high selectivity against 3,4-dihydroxyphenylacetic acid and ascorbic acid, two major chemical interferents in the brain, yielding a sensitivity of 85.40 ± 14.30 nA/μM and limit of detection of 5.8 ± 0.9 nM for DA while maintaining selectivity. Recorded in vivo by CBM-FSCV, pharmacological inhibition of DA reuptake (nomifensine) resulted in a 235 ± 60 nM increase in tonic extracellular DA concentrations, while inhibition of DA synthesis (α-methyl-dl-tyrosine) resulted in a 72.5 ± 4.8 nM decrease in DA concentrations over a 2 h period. This study showed that CBM-FSCV may serve as a unique voltammetric technique to monitor relatively slow changes in tonic extracellular DA concentrations in vivo over a prolonged time period. |
doi_str_mv | 10.1021/acs.analchem.6b02605 |
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Conventional fast-scan cyclic voltammetry (FSCV), in combination with carbon fiber microelectrodes, has been used to measure phasic DA release in vivo by adopting a background subtraction procedure to remove background capacitive currents. However, measuring tonic changes in DA concentrations using conventional FSCV has been difficult because background capacitive currents are inherently unstable over long recording periods. To measure tonic changes in DA concentrations over several hours, we applied a novel charge-balancing multiple waveform FSCV (CBM-FSCV), combined with a dual background subtraction technique, to minimize temporal variations in background capacitive currents. Using this method, in vitro, charge variations from a reference time point were nearly zero for 48 h, whereas with conventional background subtraction, charge variations progressively increased. CBM-FSCV also demonstrated a high selectivity against 3,4-dihydroxyphenylacetic acid and ascorbic acid, two major chemical interferents in the brain, yielding a sensitivity of 85.40 ± 14.30 nA/μM and limit of detection of 5.8 ± 0.9 nM for DA while maintaining selectivity. Recorded in vivo by CBM-FSCV, pharmacological inhibition of DA reuptake (nomifensine) resulted in a 235 ± 60 nM increase in tonic extracellular DA concentrations, while inhibition of DA synthesis (α-methyl-dl-tyrosine) resulted in a 72.5 ± 4.8 nM decrease in DA concentrations over a 2 h period. This study showed that CBM-FSCV may serve as a unique voltammetric technique to monitor relatively slow changes in tonic extracellular DA concentrations in vivo over a prolonged time period.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.6b02605</identifier><identifier>PMID: 27774784</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Carbon fibers ; Dopamine ; Electrodes ; In vivo methods and tests ; Inhibition ; Monitors ; Neurons ; Selectivity ; Subtraction ; Voltammetry ; Waveforms</subject><ispartof>Analytical chemistry (Washington), 2016-11, Vol.88 (22), p.10962-10970</ispartof><rights>Copyright © 2016 American Chemical Society</rights><rights>Copyright American Chemical Society Nov 15, 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a409t-889d1f30b66c5bfcc73f889f74a50a0844aae59897a54bdfbea1a8a9cf0cfe493</citedby><cites>FETCH-LOGICAL-a409t-889d1f30b66c5bfcc73f889f74a50a0844aae59897a54bdfbea1a8a9cf0cfe493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.6b02605$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.6b02605$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56717,56767</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27774784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oh, Yoonbae</creatorcontrib><creatorcontrib>Park, Cheonho</creatorcontrib><creatorcontrib>Kim, Do Hyoung</creatorcontrib><creatorcontrib>Shin, Hojin</creatorcontrib><creatorcontrib>Kang, Yu Min</creatorcontrib><creatorcontrib>DeWaele, Mark</creatorcontrib><creatorcontrib>Lee, Jeyeon</creatorcontrib><creatorcontrib>Min, Hoon-Ki</creatorcontrib><creatorcontrib>Blaha, Charles D</creatorcontrib><creatorcontrib>Bennet, Kevin E</creatorcontrib><creatorcontrib>Kim, In Young</creatorcontrib><creatorcontrib>Lee, Kendall H</creatorcontrib><creatorcontrib>Jang, Dong Pyo</creatorcontrib><title>Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Dopamine (DA) modulates central neuronal activity through both phasic (second to second) and tonic (minutes to hours) terminal release. Conventional fast-scan cyclic voltammetry (FSCV), in combination with carbon fiber microelectrodes, has been used to measure phasic DA release in vivo by adopting a background subtraction procedure to remove background capacitive currents. However, measuring tonic changes in DA concentrations using conventional FSCV has been difficult because background capacitive currents are inherently unstable over long recording periods. To measure tonic changes in DA concentrations over several hours, we applied a novel charge-balancing multiple waveform FSCV (CBM-FSCV), combined with a dual background subtraction technique, to minimize temporal variations in background capacitive currents. Using this method, in vitro, charge variations from a reference time point were nearly zero for 48 h, whereas with conventional background subtraction, charge variations progressively increased. CBM-FSCV also demonstrated a high selectivity against 3,4-dihydroxyphenylacetic acid and ascorbic acid, two major chemical interferents in the brain, yielding a sensitivity of 85.40 ± 14.30 nA/μM and limit of detection of 5.8 ± 0.9 nM for DA while maintaining selectivity. Recorded in vivo by CBM-FSCV, pharmacological inhibition of DA reuptake (nomifensine) resulted in a 235 ± 60 nM increase in tonic extracellular DA concentrations, while inhibition of DA synthesis (α-methyl-dl-tyrosine) resulted in a 72.5 ± 4.8 nM decrease in DA concentrations over a 2 h period. This study showed that CBM-FSCV may serve as a unique voltammetric technique to monitor relatively slow changes in tonic extracellular DA concentrations in vivo over a prolonged time period.</description><subject>Analytical chemistry</subject><subject>Carbon fibers</subject><subject>Dopamine</subject><subject>Electrodes</subject><subject>In vivo methods and tests</subject><subject>Inhibition</subject><subject>Monitors</subject><subject>Neurons</subject><subject>Selectivity</subject><subject>Subtraction</subject><subject>Voltammetry</subject><subject>Waveforms</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkUFv0zAYhi0EYl3hHyBkiQuXlM-JHSdHKBtM6sSBMY7RF9fuMjl2sZOKnvnjc9RukzhMO1n6_LzvJ_sh5B2DBYOcfUIVF-jQqhvdL8oW8hLECzJjIoesrKr8JZkBQJHlEuCEnMZ4C8AYsPI1OcmllFxWfEb-XXrXDT50bkMvHL3udp4ub9BtdKSdo1fpVtGzv0NApa0dLQb61W-x75ymK73Tlrb7KRA2OvuCFp2ami5HO3Rbq-lv3GnjQ0_PMQ7ZT4WOLvfKps5rbwfsez2E_RvyyqCN-u3xnJNf52dXy-_Z6se3i-XnVYYc6iGrqnrNTAFtWSrRGqVkYdLMSI4CECrOEbWoq1qi4O3atBoZVlgrA8poXhdz8vHQuw3-z6jj0PRdnJ6FTvsxNqwquSjKUrJnoIUQLBeSJ_TDf-itH0MSM1G8SB_OEz0n_ECp4GMM2jTb0PUY9g2DZvLZJJ_Nvc_m6DPF3h_Lx7bX64fQvcAEwAGY4o-Ln-q8A4dXsFI</recordid><startdate>20161115</startdate><enddate>20161115</enddate><creator>Oh, Yoonbae</creator><creator>Park, Cheonho</creator><creator>Kim, Do Hyoung</creator><creator>Shin, Hojin</creator><creator>Kang, Yu Min</creator><creator>DeWaele, Mark</creator><creator>Lee, Jeyeon</creator><creator>Min, Hoon-Ki</creator><creator>Blaha, Charles D</creator><creator>Bennet, Kevin E</creator><creator>Kim, In Young</creator><creator>Lee, Kendall H</creator><creator>Jang, Dong Pyo</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20161115</creationdate><title>Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry</title><author>Oh, Yoonbae ; Park, Cheonho ; Kim, Do Hyoung ; Shin, Hojin ; Kang, Yu Min ; DeWaele, Mark ; Lee, Jeyeon ; Min, Hoon-Ki ; Blaha, Charles D ; Bennet, Kevin E ; Kim, In Young ; Lee, Kendall H ; Jang, Dong Pyo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-889d1f30b66c5bfcc73f889f74a50a0844aae59897a54bdfbea1a8a9cf0cfe493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analytical chemistry</topic><topic>Carbon fibers</topic><topic>Dopamine</topic><topic>Electrodes</topic><topic>In vivo methods and tests</topic><topic>Inhibition</topic><topic>Monitors</topic><topic>Neurons</topic><topic>Selectivity</topic><topic>Subtraction</topic><topic>Voltammetry</topic><topic>Waveforms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oh, Yoonbae</creatorcontrib><creatorcontrib>Park, Cheonho</creatorcontrib><creatorcontrib>Kim, Do Hyoung</creatorcontrib><creatorcontrib>Shin, Hojin</creatorcontrib><creatorcontrib>Kang, Yu Min</creatorcontrib><creatorcontrib>DeWaele, Mark</creatorcontrib><creatorcontrib>Lee, Jeyeon</creatorcontrib><creatorcontrib>Min, Hoon-Ki</creatorcontrib><creatorcontrib>Blaha, Charles D</creatorcontrib><creatorcontrib>Bennet, Kevin E</creatorcontrib><creatorcontrib>Kim, In Young</creatorcontrib><creatorcontrib>Lee, Kendall H</creatorcontrib><creatorcontrib>Jang, Dong Pyo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oh, Yoonbae</au><au>Park, Cheonho</au><au>Kim, Do Hyoung</au><au>Shin, Hojin</au><au>Kang, Yu Min</au><au>DeWaele, Mark</au><au>Lee, Jeyeon</au><au>Min, Hoon-Ki</au><au>Blaha, Charles D</au><au>Bennet, Kevin E</au><au>Kim, In Young</au><au>Lee, Kendall H</au><au>Jang, Dong Pyo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2016-11-15</date><risdate>2016</risdate><volume>88</volume><issue>22</issue><spage>10962</spage><epage>10970</epage><pages>10962-10970</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Dopamine (DA) modulates central neuronal activity through both phasic (second to second) and tonic (minutes to hours) terminal release. Conventional fast-scan cyclic voltammetry (FSCV), in combination with carbon fiber microelectrodes, has been used to measure phasic DA release in vivo by adopting a background subtraction procedure to remove background capacitive currents. However, measuring tonic changes in DA concentrations using conventional FSCV has been difficult because background capacitive currents are inherently unstable over long recording periods. To measure tonic changes in DA concentrations over several hours, we applied a novel charge-balancing multiple waveform FSCV (CBM-FSCV), combined with a dual background subtraction technique, to minimize temporal variations in background capacitive currents. Using this method, in vitro, charge variations from a reference time point were nearly zero for 48 h, whereas with conventional background subtraction, charge variations progressively increased. CBM-FSCV also demonstrated a high selectivity against 3,4-dihydroxyphenylacetic acid and ascorbic acid, two major chemical interferents in the brain, yielding a sensitivity of 85.40 ± 14.30 nA/μM and limit of detection of 5.8 ± 0.9 nM for DA while maintaining selectivity. Recorded in vivo by CBM-FSCV, pharmacological inhibition of DA reuptake (nomifensine) resulted in a 235 ± 60 nM increase in tonic extracellular DA concentrations, while inhibition of DA synthesis (α-methyl-dl-tyrosine) resulted in a 72.5 ± 4.8 nM decrease in DA concentrations over a 2 h period. This study showed that CBM-FSCV may serve as a unique voltammetric technique to monitor relatively slow changes in tonic extracellular DA concentrations in vivo over a prolonged time period.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>27774784</pmid><doi>10.1021/acs.analchem.6b02605</doi><tpages>9</tpages></addata></record> |
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subjects | Analytical chemistry Carbon fibers Dopamine Electrodes In vivo methods and tests Inhibition Monitors Neurons Selectivity Subtraction Voltammetry Waveforms |
title | Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry |
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