Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol
Silk fabrics from Bombyx mori silkworm were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as a binary system of HEMA and 4‐hydroxybutyl acrylate (HBA) and then analysed by Raman and infrared (IR) spectroscopy to elucidate the interactions between the components and their possible conformat...
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Veröffentlicht in: | Journal of Raman spectroscopy 2016-11, Vol.47 (11), p.1367-1374 |
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description | Silk fabrics from Bombyx mori silkworm were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as a binary system of HEMA and 4‐hydroxybutyl acrylate (HBA) and then analysed by Raman and infrared (IR) spectroscopy to elucidate the interactions between the components and their possible conformational changes. The samples were then dissolved in trifluoroacetic acid and electrospun; the influence of the grafted polymers on the silk fibroin rearrangements upon these treatments was investigated by vibrational spectroscopy. Upon grafting, the fabrics underwent conformational rearrangements towards a more unordered state, although they kept their prevailing β‐sheet conformation; also the polymeric component underwent hydrogen bonding and backbone rearrangements upon interaction with silk fibroin and the occurrence of strong covalent bonds cannot be excluded. By immersing the as‐electrospun grafted and pure fibroin nanofibres (prevalently unordered) in aqueous methanol, they partially recovered the β‐sheet content observed in the corresponding starting fabrics; the percentage of recovery decreased along the series: pure silk > HEMA‐grafted silk > HEMA and HBA‐grafted silk. This trend suggests that the presence of the polyHEMA grafted component hinders the silk fibroin recrystallization into β‐sheet upon aqueous methanol treatment; moreover, the addition of the more sterically hindered HBA monomer in the grafting system further prevented this process. Copyright © 2016 John Wiley & Sons, Ltd.
Silk fibroin (SF) fabrics were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as with HEMA and 4‐hydroxybutyl acrylate (HBA) and then characterized by Raman and IR spectroscopy. They were electrospun from trifluoroacetic acid and then immersed in aqueous methanol. Upon grafting, SF kept its prevailing β‐sheet conformation, while had an unordered/Silk I structure in electrospun nanofibres. PolyHEMA hinders the SF recrystallization into β‐sheet upon aqueous methanol treatment; the more sterically hindered HBA component further prevented this process. |
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Silk fibroin (SF) fabrics were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as with HEMA and 4‐hydroxybutyl acrylate (HBA) and then characterized by Raman and IR spectroscopy. They were electrospun from trifluoroacetic acid and then immersed in aqueous methanol. Upon grafting, SF kept its prevailing β‐sheet conformation, while had an unordered/Silk I structure in electrospun nanofibres. PolyHEMA hinders the SF recrystallization into β‐sheet upon aqueous methanol treatment; the more sterically hindered HBA component further prevented this process.</description><identifier>ISSN: 0377-0486</identifier><identifier>EISSN: 1097-4555</identifier><identifier>DOI: 10.1002/jrs.4974</identifier><identifier>CODEN: JRSPAF</identifier><language>eng</language><publisher>Bognor Regis: Blackwell Publishing Ltd</publisher><subject>2-hydroxyethyl methacrylate ; 4-hydroxybutyl acrylate ; Acrylates ; B. mori silk fibroin ; Electrospinning ; Fabrics ; Grafting ; Infrared spectroscopy ; Methyl alcohol ; Silk fibroin ; Textile composites ; vibrational spectroscopy</subject><ispartof>Journal of Raman spectroscopy, 2016-11, Vol.47 (11), p.1367-1374</ispartof><rights>Copyright © 2016 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3644-b018b5647911c683fc089e37301f2a698592163c647eabd55d2c2c7fb53d01613</citedby><cites>FETCH-LOGICAL-c3644-b018b5647911c683fc089e37301f2a698592163c647eabd55d2c2c7fb53d01613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjrs.4974$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjrs.4974$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Pavoni, Eleonora</creatorcontrib><creatorcontrib>Tsukada, Masuhiro</creatorcontrib><creatorcontrib>Taddei, Paola</creatorcontrib><title>Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol</title><title>Journal of Raman spectroscopy</title><addtitle>J. Raman Spectrosc</addtitle><description>Silk fabrics from Bombyx mori silkworm were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as a binary system of HEMA and 4‐hydroxybutyl acrylate (HBA) and then analysed by Raman and infrared (IR) spectroscopy to elucidate the interactions between the components and their possible conformational changes. The samples were then dissolved in trifluoroacetic acid and electrospun; the influence of the grafted polymers on the silk fibroin rearrangements upon these treatments was investigated by vibrational spectroscopy. Upon grafting, the fabrics underwent conformational rearrangements towards a more unordered state, although they kept their prevailing β‐sheet conformation; also the polymeric component underwent hydrogen bonding and backbone rearrangements upon interaction with silk fibroin and the occurrence of strong covalent bonds cannot be excluded. By immersing the as‐electrospun grafted and pure fibroin nanofibres (prevalently unordered) in aqueous methanol, they partially recovered the β‐sheet content observed in the corresponding starting fabrics; the percentage of recovery decreased along the series: pure silk > HEMA‐grafted silk > HEMA and HBA‐grafted silk. This trend suggests that the presence of the polyHEMA grafted component hinders the silk fibroin recrystallization into β‐sheet upon aqueous methanol treatment; moreover, the addition of the more sterically hindered HBA monomer in the grafting system further prevented this process. Copyright © 2016 John Wiley & Sons, Ltd.
Silk fibroin (SF) fabrics were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as with HEMA and 4‐hydroxybutyl acrylate (HBA) and then characterized by Raman and IR spectroscopy. They were electrospun from trifluoroacetic acid and then immersed in aqueous methanol. Upon grafting, SF kept its prevailing β‐sheet conformation, while had an unordered/Silk I structure in electrospun nanofibres. PolyHEMA hinders the SF recrystallization into β‐sheet upon aqueous methanol treatment; the more sterically hindered HBA component further prevented this process.</description><subject>2-hydroxyethyl methacrylate</subject><subject>4-hydroxybutyl acrylate</subject><subject>Acrylates</subject><subject>B. mori silk fibroin</subject><subject>Electrospinning</subject><subject>Fabrics</subject><subject>Grafting</subject><subject>Infrared spectroscopy</subject><subject>Methyl alcohol</subject><subject>Silk fibroin</subject><subject>Textile composites</subject><subject>vibrational spectroscopy</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kc2KFDEUhYMo2I6CjxBw46bGpPJXtZRR25FGQVtchlQq6U5PKimTFGM_iy9rihkUBcniQvjOuT8HgOcYXWKE2lenlC9pL-gDsMGoFw1ljD0EG0SEaBDt-GPwJOcTQqjvOd6An9fB-sUEbWC08JCULS4c4K0rR6h0OntVDNRxmuMSxgxjgOW4fgQb06SKi0F5mIxKSYWDmUwoeTXKzt9A64YUXYDLXGXGG11SzLMLYe2gwghLFZZVc9_v-2LikuFkylGF6J-CR1b5bJ7d1wvw9d3b_dX7Zvdpe331etdowiltBoS7gXEqeow174jVqOsNEQRh2yred6xvMSe6EkYNI2Njq1st7MDIiDDH5AK8vPOdU6wj5CInl7XxXoV1Hok7TlnbdwJV9MU_6Ckuqd5gpWh9LcLkj6GuC-dkrJyTm1Q6S4zkmpKsKck1pYo2d-it8-b8X05--Pzlb97lYn785lW6kVwQweS3j1tJ3-x3-y3ayZb8AjC8pZU</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Pavoni, Eleonora</creator><creator>Tsukada, Masuhiro</creator><creator>Taddei, Paola</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201611</creationdate><title>Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol</title><author>Pavoni, Eleonora ; Tsukada, Masuhiro ; Taddei, Paola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3644-b018b5647911c683fc089e37301f2a698592163c647eabd55d2c2c7fb53d01613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>2-hydroxyethyl methacrylate</topic><topic>4-hydroxybutyl acrylate</topic><topic>Acrylates</topic><topic>B. mori silk fibroin</topic><topic>Electrospinning</topic><topic>Fabrics</topic><topic>Grafting</topic><topic>Infrared spectroscopy</topic><topic>Methyl alcohol</topic><topic>Silk fibroin</topic><topic>Textile composites</topic><topic>vibrational spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pavoni, Eleonora</creatorcontrib><creatorcontrib>Tsukada, Masuhiro</creatorcontrib><creatorcontrib>Taddei, Paola</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of Raman spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pavoni, Eleonora</au><au>Tsukada, Masuhiro</au><au>Taddei, Paola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol</atitle><jtitle>Journal of Raman spectroscopy</jtitle><addtitle>J. Raman Spectrosc</addtitle><date>2016-11</date><risdate>2016</risdate><volume>47</volume><issue>11</issue><spage>1367</spage><epage>1374</epage><pages>1367-1374</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><coden>JRSPAF</coden><abstract>Silk fabrics from Bombyx mori silkworm were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as a binary system of HEMA and 4‐hydroxybutyl acrylate (HBA) and then analysed by Raman and infrared (IR) spectroscopy to elucidate the interactions between the components and their possible conformational changes. The samples were then dissolved in trifluoroacetic acid and electrospun; the influence of the grafted polymers on the silk fibroin rearrangements upon these treatments was investigated by vibrational spectroscopy. Upon grafting, the fabrics underwent conformational rearrangements towards a more unordered state, although they kept their prevailing β‐sheet conformation; also the polymeric component underwent hydrogen bonding and backbone rearrangements upon interaction with silk fibroin and the occurrence of strong covalent bonds cannot be excluded. By immersing the as‐electrospun grafted and pure fibroin nanofibres (prevalently unordered) in aqueous methanol, they partially recovered the β‐sheet content observed in the corresponding starting fabrics; the percentage of recovery decreased along the series: pure silk > HEMA‐grafted silk > HEMA and HBA‐grafted silk. This trend suggests that the presence of the polyHEMA grafted component hinders the silk fibroin recrystallization into β‐sheet upon aqueous methanol treatment; moreover, the addition of the more sterically hindered HBA monomer in the grafting system further prevented this process. Copyright © 2016 John Wiley & Sons, Ltd.
Silk fibroin (SF) fabrics were grafted with 2‐hydroxyethyl methacrylate (HEMA) as well as with HEMA and 4‐hydroxybutyl acrylate (HBA) and then characterized by Raman and IR spectroscopy. They were electrospun from trifluoroacetic acid and then immersed in aqueous methanol. Upon grafting, SF kept its prevailing β‐sheet conformation, while had an unordered/Silk I structure in electrospun nanofibres. PolyHEMA hinders the SF recrystallization into β‐sheet upon aqueous methanol treatment; the more sterically hindered HBA component further prevented this process.</abstract><cop>Bognor Regis</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1002/jrs.4974</doi><tpages>8</tpages></addata></record> |
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subjects | 2-hydroxyethyl methacrylate 4-hydroxybutyl acrylate Acrylates B. mori silk fibroin Electrospinning Fabrics Grafting Infrared spectroscopy Methyl alcohol Silk fibroin Textile composites vibrational spectroscopy |
title | Influence of grafting with acrylate compounds on the conformational rearrangements of silk fibroin upon electrospinning and treatment with aqueous methanol |
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