A butyrylcholinesterase in the early development of the brine shrimp ( Artemia salina) larvae: a target for phthalate ester embryotoxicity?
The phthalate ester insensitive blue-green algae ( Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp ( Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development....
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| Veröffentlicht in: | Biochemical and biophysical research communications 2002-12, Vol.299 (4), p.659-662 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Acey, Roger A Bailey, Stacie Healy, Patricia Jo, Chang Unger, Thomas F Hudson, Richard A |
| description | The phthalate ester insensitive blue-green algae (
Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp (
Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development. The maximum acute toxicity due to di-
n-butyl phthalate (DNBP) correlated with the expression of a phthalate ester-hydrolyzing enzyme. The purified enzyme was identified as a butyrylcholinesterase due to its rapid inactivation by low concentrations (10
−7
M) of diisopropyl fluorophosphate and inhibition by physostigmine (IC
50=6×10
−7
M) and tetraisopropylpyrophosphoramide (I-OMPA, IC
50=5×10
−6
M) but not by BW284c5. Apparently competition of the phthalates with the endogenous substrates of the enzyme led to development-dependent toxicity. |
| doi_str_mv | 10.1016/S0006-291X(02)02716-X |
| format | Article |
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Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp (
Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development. The maximum acute toxicity due to di-
n-butyl phthalate (DNBP) correlated with the expression of a phthalate ester-hydrolyzing enzyme. The purified enzyme was identified as a butyrylcholinesterase due to its rapid inactivation by low concentrations (10
−7
M) of diisopropyl fluorophosphate and inhibition by physostigmine (IC
50=6×10
−7
M) and tetraisopropylpyrophosphoramide (I-OMPA, IC
50=5×10
−6
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Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp (
Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development. The maximum acute toxicity due to di-
n-butyl phthalate (DNBP) correlated with the expression of a phthalate ester-hydrolyzing enzyme. The purified enzyme was identified as a butyrylcholinesterase due to its rapid inactivation by low concentrations (10
−7
M) of diisopropyl fluorophosphate and inhibition by physostigmine (IC
50=6×10
−7
M) and tetraisopropylpyrophosphoramide (I-OMPA, IC
50=5×10
−6
M) but not by BW284c5. Apparently competition of the phthalates with the endogenous substrates of the enzyme led to development-dependent toxicity.</description><subject>Animals</subject><subject>Artemia - drug effects</subject><subject>Artemia - enzymology</subject><subject>Artemia - growth & development</subject><subject>Artemia salina</subject><subject>Brackish</subject><subject>butyrylcholinesterase</subject><subject>Butyrylcholinesterase - metabolism</subject><subject>Cyanobacteria - metabolism</subject><subject>Diet</subject><subject>Esters</subject><subject>Larva - drug effects</subject><subject>Larva - metabolism</subject><subject>Marine</subject><subject>Molecular Structure</subject><subject>n-Butyl phthalate</subject><subject>Phthalic Acids - metabolism</subject><subject>Phthalic Acids - toxicity</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQhS0EopfCTwB5hdpF6IzzqtlUVxUvqVIXBenuLMeZEKMkDrbvVfMb-qfxfQiWrLyYb87xmcPYW4QPCFhdPQBAlQmJmwsQlyBqrLLNM7ZCkJAJhOI5W_1FztirEH4BIBaVfMnOUBSlRAkr9rTmzTYufhlM7wY7UYjkdSBuJx574qT9sPCWdjS4eaQpctcdBo1PMA-9t-PML_jaRxqt5kEnEX3JB-13mj5yzaP2Pynyznk-97HXg45Jdm_DaWz84qJ7tMbG5eY1e9HpIdCb03vOfnz-9P32a3Z3_-Xb7fouM7kUMStAFjnVdYpClS6hEZURDZiiK0SO2KEwlchFp-tSCJMLKEsUsq11i60013V-zt4fdWfvfm_TV9Rog6Fh0BO5bVB4XRVY5nkCyyNovAvBU6fmFFf7RSGofQvq0ILan1iBUIcW1CbtvTsZbJuR2n9bp7Mn4OYIUIq5s-RVMJYmQ631ZKJqnf2PxR-y5pjh</recordid><startdate>20021213</startdate><enddate>20021213</enddate><creator>Acey, Roger A</creator><creator>Bailey, Stacie</creator><creator>Healy, Patricia</creator><creator>Jo, Chang</creator><creator>Unger, Thomas F</creator><creator>Hudson, Richard A</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20021213</creationdate><title>A butyrylcholinesterase in the early development of the brine shrimp ( Artemia salina) larvae: a target for phthalate ester embryotoxicity?</title><author>Acey, Roger A ; Bailey, Stacie ; Healy, Patricia ; Jo, Chang ; Unger, Thomas F ; Hudson, Richard A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-40943e77469e6a50b26c2b0c4f42311f12c6232fa7522c32055129d7ad1d9c873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Artemia - drug effects</topic><topic>Artemia - enzymology</topic><topic>Artemia - growth & development</topic><topic>Artemia salina</topic><topic>Brackish</topic><topic>butyrylcholinesterase</topic><topic>Butyrylcholinesterase - metabolism</topic><topic>Cyanobacteria - metabolism</topic><topic>Diet</topic><topic>Esters</topic><topic>Larva - drug effects</topic><topic>Larva - metabolism</topic><topic>Marine</topic><topic>Molecular Structure</topic><topic>n-Butyl phthalate</topic><topic>Phthalic Acids - metabolism</topic><topic>Phthalic Acids - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Acey, Roger A</creatorcontrib><creatorcontrib>Bailey, Stacie</creatorcontrib><creatorcontrib>Healy, Patricia</creatorcontrib><creatorcontrib>Jo, Chang</creatorcontrib><creatorcontrib>Unger, Thomas F</creatorcontrib><creatorcontrib>Hudson, Richard A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Acey, Roger A</au><au>Bailey, Stacie</au><au>Healy, Patricia</au><au>Jo, Chang</au><au>Unger, Thomas F</au><au>Hudson, Richard A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A butyrylcholinesterase in the early development of the brine shrimp ( Artemia salina) larvae: a target for phthalate ester embryotoxicity?</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-12-13</date><risdate>2002</risdate><volume>299</volume><issue>4</issue><spage>659</spage><epage>662</epage><pages>659-662</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The phthalate ester insensitive blue-green algae (
Synechococcus lividus) were used as a food source to extend the survival of synchronously hatched brine shrimp (
Artemia salina) larvae allowing measurement of a reduced toxic response to phthalate esters at late post-hatching stages of development. The maximum acute toxicity due to di-
n-butyl phthalate (DNBP) correlated with the expression of a phthalate ester-hydrolyzing enzyme. The purified enzyme was identified as a butyrylcholinesterase due to its rapid inactivation by low concentrations (10
−7
M) of diisopropyl fluorophosphate and inhibition by physostigmine (IC
50=6×10
−7
M) and tetraisopropylpyrophosphoramide (I-OMPA, IC
50=5×10
−6
M) but not by BW284c5. Apparently competition of the phthalates with the endogenous substrates of the enzyme led to development-dependent toxicity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12459190</pmid><doi>10.1016/S0006-291X(02)02716-X</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 2002-12, Vol.299 (4), p.659-662 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Artemia - drug effects Artemia - enzymology Artemia - growth & development Artemia salina Brackish butyrylcholinesterase Butyrylcholinesterase - metabolism Cyanobacteria - metabolism Diet Esters Larva - drug effects Larva - metabolism Marine Molecular Structure n-Butyl phthalate Phthalic Acids - metabolism Phthalic Acids - toxicity |
| title | A butyrylcholinesterase in the early development of the brine shrimp ( Artemia salina) larvae: a target for phthalate ester embryotoxicity? |
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