The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE
In this study, the prevalence of Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-μm pore size membrane filters...
Gespeichert in:
| Veröffentlicht in: | International journal of food microbiology 2003-02, Vol.81 (1), p.21-28 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 28 |
|---|---|
| container_issue | 1 |
| container_start_page | 21 |
| container_title | International journal of food microbiology |
| container_volume | 81 |
| creator | Atabay, H.Ibrahim Aydin, Fuat Houf, Kurt Sahin, Mitat Vandamme, Peter |
| description | In this study, the prevalence of
Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-μm pore size membrane filters laid onto a nonselective blood agar was used after enrichment in Oxoid
Arcobacter Enrichment Broth (AEB) to examine a total of 75 chicken carcasses (44 fresh and 31 frozen). Species level identification was performed using SDS-PAGE of whole-cell proteins and a recently developed multiplex-PCR assay. All isolates were identified as
Arcobacter butzleri. Of the 44 fresh chicken carcasses examined, 42 (95%) were positive for
A. butzleri.
A. butzleri was also recovered from seven (23%) of the 31 frozen carcasses examined. |
| doi_str_mv | 10.1016/S0168-1605(02)00163-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18637762</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168160502001630</els_id><sourcerecordid>18637762</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-95fcbe90fe15ffa076a32bce8679645982d721b0b4bf87a480194e2b4aab6d033</originalsourceid><addsrcrecordid>eNqFkd1u1DAQhS0EokvhEUC-AYFEytiO83OFVqUUpEog7XJtTZwxNc0mwXYq9RV4arzdFb3kxmNb35yxz2HspYAzAaL6sMlLU4gK9FuQ7yCfVAGP2Eo0dVuosoLHbPUPOWHPYvwFAFopeMpOhCylaoVesT_ba-JzoFscaLTEJ8fXwU4d2kSBx3k-49PI7bW3N5QrBosxUuRxGnruRx4ooR_4DsMNpbi_2S55e_ee45iBnsbknbeYfJbJ4imP87kZUxZZoh9_8s2nTfF9fXnxnD1xOER6cayn7Mfni-35l-Lq2-XX8_VVYUspU9FqZztqwZHQziHUFSrZWWqquq1K3Tayr6XooCs719RYNiDakmRXInZVD0qdsjcH3TlMvxeKyex8tDQMONK0RCOaStV1JTOoD6ANU4yBnJmDzz-9MwLMPgRzH4LZO2xAmvsQDOS-V8cBS7ej_qHr6HoGXh8BjBYHF3C0Pj5wWqhGQ525jweOsh23noKJ1u9j6n0gm0w_-f885S8viKQR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18637762</pqid></control><display><type>article</type><title>The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Atabay, H.Ibrahim ; Aydin, Fuat ; Houf, Kurt ; Sahin, Mitat ; Vandamme, Peter</creator><creatorcontrib>Atabay, H.Ibrahim ; Aydin, Fuat ; Houf, Kurt ; Sahin, Mitat ; Vandamme, Peter</creatorcontrib><description>In this study, the prevalence of
Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-μm pore size membrane filters laid onto a nonselective blood agar was used after enrichment in Oxoid
Arcobacter Enrichment Broth (AEB) to examine a total of 75 chicken carcasses (44 fresh and 31 frozen). Species level identification was performed using SDS-PAGE of whole-cell proteins and a recently developed multiplex-PCR assay. All isolates were identified as
Arcobacter butzleri. Of the 44 fresh chicken carcasses examined, 42 (95%) were positive for
A. butzleri.
A. butzleri was also recovered from seven (23%) of the 31 frozen carcasses examined.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/S0168-1605(02)00163-0</identifier><identifier>PMID: 12423915</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Arcobacter ; Arcobacter - classification ; Arcobacter - genetics ; Arcobacter - isolation & purification ; Bacterial Proteins - analysis ; Biological and medical sciences ; Chickens - microbiology ; Electrophoresis, Polyacrylamide Gel - methods ; Food Contamination ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Meat and meat product industries ; Multiplex-PCR ; Phenotype ; Polymerase Chain Reaction - methods ; Poultry ; Prevalence ; SDS-PAGE ; Turkey - epidemiology</subject><ispartof>International journal of food microbiology, 2003-02, Vol.81 (1), p.21-28</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-95fcbe90fe15ffa076a32bce8679645982d721b0b4bf87a480194e2b4aab6d033</citedby><cites>FETCH-LOGICAL-c422t-95fcbe90fe15ffa076a32bce8679645982d721b0b4bf87a480194e2b4aab6d033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-1605(02)00163-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15138507$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12423915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atabay, H.Ibrahim</creatorcontrib><creatorcontrib>Aydin, Fuat</creatorcontrib><creatorcontrib>Houf, Kurt</creatorcontrib><creatorcontrib>Sahin, Mitat</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><title>The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>In this study, the prevalence of
Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-μm pore size membrane filters laid onto a nonselective blood agar was used after enrichment in Oxoid
Arcobacter Enrichment Broth (AEB) to examine a total of 75 chicken carcasses (44 fresh and 31 frozen). Species level identification was performed using SDS-PAGE of whole-cell proteins and a recently developed multiplex-PCR assay. All isolates were identified as
Arcobacter butzleri. Of the 44 fresh chicken carcasses examined, 42 (95%) were positive for
A. butzleri.
A. butzleri was also recovered from seven (23%) of the 31 frozen carcasses examined.</description><subject>Animals</subject><subject>Arcobacter</subject><subject>Arcobacter - classification</subject><subject>Arcobacter - genetics</subject><subject>Arcobacter - isolation & purification</subject><subject>Bacterial Proteins - analysis</subject><subject>Biological and medical sciences</subject><subject>Chickens - microbiology</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Food Contamination</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Meat and meat product industries</subject><subject>Multiplex-PCR</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Poultry</subject><subject>Prevalence</subject><subject>SDS-PAGE</subject><subject>Turkey - epidemiology</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd1u1DAQhS0EokvhEUC-AYFEytiO83OFVqUUpEog7XJtTZwxNc0mwXYq9RV4arzdFb3kxmNb35yxz2HspYAzAaL6sMlLU4gK9FuQ7yCfVAGP2Eo0dVuosoLHbPUPOWHPYvwFAFopeMpOhCylaoVesT_ba-JzoFscaLTEJ8fXwU4d2kSBx3k-49PI7bW3N5QrBosxUuRxGnruRx4ooR_4DsMNpbi_2S55e_ee45iBnsbknbeYfJbJ4imP87kZUxZZoh9_8s2nTfF9fXnxnD1xOER6cayn7Mfni-35l-Lq2-XX8_VVYUspU9FqZztqwZHQziHUFSrZWWqquq1K3Tayr6XooCs719RYNiDakmRXInZVD0qdsjcH3TlMvxeKyex8tDQMONK0RCOaStV1JTOoD6ANU4yBnJmDzz-9MwLMPgRzH4LZO2xAmvsQDOS-V8cBS7ej_qHr6HoGXh8BjBYHF3C0Pj5wWqhGQ525jweOsh23noKJ1u9j6n0gm0w_-f885S8viKQR</recordid><startdate>20030225</startdate><enddate>20030225</enddate><creator>Atabay, H.Ibrahim</creator><creator>Aydin, Fuat</creator><creator>Houf, Kurt</creator><creator>Sahin, Mitat</creator><creator>Vandamme, Peter</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7T7</scope><scope>7U2</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20030225</creationdate><title>The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE</title><author>Atabay, H.Ibrahim ; Aydin, Fuat ; Houf, Kurt ; Sahin, Mitat ; Vandamme, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-95fcbe90fe15ffa076a32bce8679645982d721b0b4bf87a480194e2b4aab6d033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Arcobacter</topic><topic>Arcobacter - classification</topic><topic>Arcobacter - genetics</topic><topic>Arcobacter - isolation & purification</topic><topic>Bacterial Proteins - analysis</topic><topic>Biological and medical sciences</topic><topic>Chickens - microbiology</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Food Contamination</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Meat and meat product industries</topic><topic>Multiplex-PCR</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Poultry</topic><topic>Prevalence</topic><topic>SDS-PAGE</topic><topic>Turkey - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atabay, H.Ibrahim</creatorcontrib><creatorcontrib>Aydin, Fuat</creatorcontrib><creatorcontrib>Houf, Kurt</creatorcontrib><creatorcontrib>Sahin, Mitat</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Safety Science and Risk</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atabay, H.Ibrahim</au><au>Aydin, Fuat</au><au>Houf, Kurt</au><au>Sahin, Mitat</au><au>Vandamme, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2003-02-25</date><risdate>2003</risdate><volume>81</volume><issue>1</issue><spage>21</spage><epage>28</epage><pages>21-28</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>In this study, the prevalence of
Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-μm pore size membrane filters laid onto a nonselective blood agar was used after enrichment in Oxoid
Arcobacter Enrichment Broth (AEB) to examine a total of 75 chicken carcasses (44 fresh and 31 frozen). Species level identification was performed using SDS-PAGE of whole-cell proteins and a recently developed multiplex-PCR assay. All isolates were identified as
Arcobacter butzleri. Of the 44 fresh chicken carcasses examined, 42 (95%) were positive for
A. butzleri.
A. butzleri was also recovered from seven (23%) of the 31 frozen carcasses examined.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12423915</pmid><doi>10.1016/S0168-1605(02)00163-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2003-02, Vol.81 (1), p.21-28 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18637762 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Arcobacter Arcobacter - classification Arcobacter - genetics Arcobacter - isolation & purification Bacterial Proteins - analysis Biological and medical sciences Chickens - microbiology Electrophoresis, Polyacrylamide Gel - methods Food Contamination Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Meat and meat product industries Multiplex-PCR Phenotype Polymerase Chain Reaction - methods Poultry Prevalence SDS-PAGE Turkey - epidemiology |
| title | The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T19%3A16%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20prevalence%20of%20Arcobacter%20spp.%20on%20chicken%20carcasses%20sold%20in%20retail%20markets%20in%20Turkey,%20and%20identification%20of%20the%20isolates%20using%20SDS-PAGE&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Atabay,%20H.Ibrahim&rft.date=2003-02-25&rft.volume=81&rft.issue=1&rft.spage=21&rft.epage=28&rft.pages=21-28&rft.issn=0168-1605&rft.eissn=1879-3460&rft.coden=IJFMDD&rft_id=info:doi/10.1016/S0168-1605(02)00163-0&rft_dat=%3Cproquest_cross%3E18637762%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18637762&rft_id=info:pmid/12423915&rft_els_id=S0168160502001630&rfr_iscdi=true |