Mesencephalic trigeminal neurons are innervated by nitric oxide synthase-containing fibers and respond to nitric oxide
In the present study we found that mesencephalic trigeminal (Mes-V) neurons of the rat are innervated by nitrergic fibers and that nitric oxide (NO) modifies the electrophysiological properties of these cells. Mes-V neurons were surrounded by a network of fibers that contained neuronal nitric oxide...
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| Veröffentlicht in: | Brain research 2003-01, Vol.960 (1), p.81-89 |
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| creator | Pose, Inés Sampogna, Sharon Chase, Michael H. Morales, Francisco R. |
| description | In the present study we found that mesencephalic trigeminal (Mes-V) neurons of the rat are innervated by nitrergic fibers and that nitric oxide (NO) modifies the electrophysiological properties of these cells. Mes-V neurons were surrounded by a network of fibers that contained neuronal nitric oxide synthase (nNOS); these fibers gave rise to terminal-, bouton-like structures which ended in Mes-V cells bodies. These cells, which did not display nNOS-like immunoreactivity were immunoreactive to a cGMP antibody. By performing intracellular recordings in the adult rat brain slice preparation, the effects of diethylenetriamine/NO adduct (DETA/NO) applications were examined. DETA/NO induced a depolarization that averaged 2.2 mV (range: 1–6 mV) in nine of 22 neurons. In 15 of 22 neurons (68% of the cells), there was a decrease in current threshold from 0.74 to 0.60 nA (19%;
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| doi_str_mv | 10.1016/S0006-8993(02)03776-9 |
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P<0.001). The excitatory effects of DETA/NO were abolished by ODQ, a blocker of soluble guanylate cyclase. Input resistance (
R
in) decreased in 80% of the cells from a mean of 24.8 to 20.6 MΩ (17%;
P<0.001) and the membrane time constant (
τ
m) decreased from 7.5 to 5.6 ms (25%;
P<0.05). The ‘sag’ seen in the membrane response of these cells to current pulses was augmented during DETA/NO application. These findings indicate that there is a nitrergic innervation of Mes-V neurons and that these sensory cells are target for NO that may act on them as an excitatory neuromodulator promoting the synthesis of intracellular cGMP.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(02)03776-9</identifier><identifier>PMID: 12505660</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Central nervous system ; Cholera Toxin ; Cyclic GMP - metabolism ; Electrophysiology ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Guanylate Cyclase - antagonists & inhibitors ; Horseradish Peroxidase ; Immunohistochemistry ; In Vitro Techniques ; Masseter Muscle - innervation ; Masseter Muscle - physiology ; Membrane Potentials - drug effects ; Mes-V nucleus ; Mesencephalon - cytology ; Mesencephalon - drug effects ; Mesencephalon - physiology ; Motor reflex ; NADPH Dehydrogenase - metabolism ; Nerve Fibers - drug effects ; Nerve Fibers - enzymology ; Neuromodulation ; Nitric oxide ; Nitric Oxide - pharmacology ; Nitric Oxide Donors - antagonists & inhibitors ; Nitric Oxide Donors - pharmacology ; Nitric Oxide Synthase - physiology ; Nitric Oxide Synthase Type I ; Polyamines - antagonists & inhibitors ; Polyamines - pharmacology ; Propioception ; Rats ; Rats, Wistar ; Sensory ; Trigeminal ; Trigeminal Nerve - drug effects ; Trigeminal Nerve - enzymology ; Trigeminal Nerve - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2003-01, Vol.960 (1), p.81-89</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-d40509d7b28c8bb2f12e0519f6928bc974c144c90e0d3bdb4903b956822fd5243</citedby><cites>FETCH-LOGICAL-c422t-d40509d7b28c8bb2f12e0519f6928bc974c144c90e0d3bdb4903b956822fd5243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(02)03776-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14423051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12505660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pose, Inés</creatorcontrib><creatorcontrib>Sampogna, Sharon</creatorcontrib><creatorcontrib>Chase, Michael H.</creatorcontrib><creatorcontrib>Morales, Francisco R.</creatorcontrib><title>Mesencephalic trigeminal neurons are innervated by nitric oxide synthase-containing fibers and respond to nitric oxide</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>In the present study we found that mesencephalic trigeminal (Mes-V) neurons of the rat are innervated by nitrergic fibers and that nitric oxide (NO) modifies the electrophysiological properties of these cells. Mes-V neurons were surrounded by a network of fibers that contained neuronal nitric oxide synthase (nNOS); these fibers gave rise to terminal-, bouton-like structures which ended in Mes-V cells bodies. These cells, which did not display nNOS-like immunoreactivity were immunoreactive to a cGMP antibody. By performing intracellular recordings in the adult rat brain slice preparation, the effects of diethylenetriamine/NO adduct (DETA/NO) applications were examined. DETA/NO induced a depolarization that averaged 2.2 mV (range: 1–6 mV) in nine of 22 neurons. In 15 of 22 neurons (68% of the cells), there was a decrease in current threshold from 0.74 to 0.60 nA (19%;
P<0.001). The excitatory effects of DETA/NO were abolished by ODQ, a blocker of soluble guanylate cyclase. Input resistance (
R
in) decreased in 80% of the cells from a mean of 24.8 to 20.6 MΩ (17%;
P<0.001) and the membrane time constant (
τ
m) decreased from 7.5 to 5.6 ms (25%;
P<0.05). The ‘sag’ seen in the membrane response of these cells to current pulses was augmented during DETA/NO application. These findings indicate that there is a nitrergic innervation of Mes-V neurons and that these sensory cells are target for NO that may act on them as an excitatory neuromodulator promoting the synthesis of intracellular cGMP.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Cholera Toxin</subject><subject>Cyclic GMP - metabolism</subject><subject>Electrophysiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guanylate Cyclase - antagonists & inhibitors</subject><subject>Horseradish Peroxidase</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Masseter Muscle - innervation</subject><subject>Masseter Muscle - physiology</subject><subject>Membrane Potentials - drug effects</subject><subject>Mes-V nucleus</subject><subject>Mesencephalon - cytology</subject><subject>Mesencephalon - drug effects</subject><subject>Mesencephalon - physiology</subject><subject>Motor reflex</subject><subject>NADPH Dehydrogenase - metabolism</subject><subject>Nerve Fibers - drug effects</subject><subject>Nerve Fibers - enzymology</subject><subject>Neuromodulation</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - pharmacology</subject><subject>Nitric Oxide Donors - antagonists & inhibitors</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Nitric Oxide Synthase - physiology</subject><subject>Nitric Oxide Synthase Type I</subject><subject>Polyamines - antagonists & inhibitors</subject><subject>Polyamines - pharmacology</subject><subject>Propioception</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sensory</subject><subject>Trigeminal</subject><subject>Trigeminal Nerve - drug effects</subject><subject>Trigeminal Nerve - enzymology</subject><subject>Trigeminal Nerve - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EomnhJ4B8AcFhYfyx3vUJoYoCUhEH4Gz5Y7Y12tjB3kTk3-M0ERUnTqORntczfoaQZwzeMGDq7TcAUN2otXgF_DWIYVCdfkBWbBx4p7iEh2T1Fzkj57X-bK0QGh6TM8Z76JWCFdl9wYrJ4-bWztHTpcQbXMdkZ5pwW3Kq1BakMSUsO7tgoG5PU2yYp_l3DEjrPi23tmLnc1psTDHd0Ck6LC2ZAi1YN7nVJf8Te0IeTXau-PRUL8iPqw_fLz91118_fr58f915yfnSBQk96DA4PvrROT4xjtAzPSnNR-f1ID2T0mtACMIFJzUIp3s1cj6FnktxQV4e392U_GuLdTHrWD3Os02Yt9WwUQnB-NDA_gj6kmstOJlNiWtb9oaBOQg3d8LNwaYBbu6EG91yz08Dtm6N4T51MtyAFyfAVm_nqdjkY73npOSifalx744cNh27iMVUHw-XCbGgX0zI8T-r_AHL3p6t</recordid><startdate>20030117</startdate><enddate>20030117</enddate><creator>Pose, Inés</creator><creator>Sampogna, Sharon</creator><creator>Chase, Michael H.</creator><creator>Morales, Francisco R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20030117</creationdate><title>Mesencephalic trigeminal neurons are innervated by nitric oxide synthase-containing fibers and respond to nitric oxide</title><author>Pose, Inés ; Sampogna, Sharon ; Chase, Michael H. ; Morales, Francisco R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-d40509d7b28c8bb2f12e0519f6928bc974c144c90e0d3bdb4903b956822fd5243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Cholera Toxin</topic><topic>Cyclic GMP - metabolism</topic><topic>Electrophysiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guanylate Cyclase - antagonists & inhibitors</topic><topic>Horseradish Peroxidase</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Masseter Muscle - innervation</topic><topic>Masseter Muscle - physiology</topic><topic>Membrane Potentials - drug effects</topic><topic>Mes-V nucleus</topic><topic>Mesencephalon - cytology</topic><topic>Mesencephalon - drug effects</topic><topic>Mesencephalon - physiology</topic><topic>Motor reflex</topic><topic>NADPH Dehydrogenase - metabolism</topic><topic>Nerve Fibers - drug effects</topic><topic>Nerve Fibers - enzymology</topic><topic>Neuromodulation</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - pharmacology</topic><topic>Nitric Oxide Donors - antagonists & inhibitors</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Nitric Oxide Synthase - physiology</topic><topic>Nitric Oxide Synthase Type I</topic><topic>Polyamines - antagonists & inhibitors</topic><topic>Polyamines - pharmacology</topic><topic>Propioception</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sensory</topic><topic>Trigeminal</topic><topic>Trigeminal Nerve - drug effects</topic><topic>Trigeminal Nerve - enzymology</topic><topic>Trigeminal Nerve - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pose, Inés</creatorcontrib><creatorcontrib>Sampogna, Sharon</creatorcontrib><creatorcontrib>Chase, Michael H.</creatorcontrib><creatorcontrib>Morales, Francisco R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pose, Inés</au><au>Sampogna, Sharon</au><au>Chase, Michael H.</au><au>Morales, Francisco R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mesencephalic trigeminal neurons are innervated by nitric oxide synthase-containing fibers and respond to nitric oxide</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2003-01-17</date><risdate>2003</risdate><volume>960</volume><issue>1</issue><spage>81</spage><epage>89</epage><pages>81-89</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>In the present study we found that mesencephalic trigeminal (Mes-V) neurons of the rat are innervated by nitrergic fibers and that nitric oxide (NO) modifies the electrophysiological properties of these cells. Mes-V neurons were surrounded by a network of fibers that contained neuronal nitric oxide synthase (nNOS); these fibers gave rise to terminal-, bouton-like structures which ended in Mes-V cells bodies. These cells, which did not display nNOS-like immunoreactivity were immunoreactive to a cGMP antibody. By performing intracellular recordings in the adult rat brain slice preparation, the effects of diethylenetriamine/NO adduct (DETA/NO) applications were examined. DETA/NO induced a depolarization that averaged 2.2 mV (range: 1–6 mV) in nine of 22 neurons. In 15 of 22 neurons (68% of the cells), there was a decrease in current threshold from 0.74 to 0.60 nA (19%;
P<0.001). The excitatory effects of DETA/NO were abolished by ODQ, a blocker of soluble guanylate cyclase. Input resistance (
R
in) decreased in 80% of the cells from a mean of 24.8 to 20.6 MΩ (17%;
P<0.001) and the membrane time constant (
τ
m) decreased from 7.5 to 5.6 ms (25%;
P<0.05). The ‘sag’ seen in the membrane response of these cells to current pulses was augmented during DETA/NO application. These findings indicate that there is a nitrergic innervation of Mes-V neurons and that these sensory cells are target for NO that may act on them as an excitatory neuromodulator promoting the synthesis of intracellular cGMP.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>12505660</pmid><doi>10.1016/S0006-8993(02)03776-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Central nervous system Cholera Toxin Cyclic GMP - metabolism Electrophysiology Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Guanylate Cyclase - antagonists & inhibitors Horseradish Peroxidase Immunohistochemistry In Vitro Techniques Masseter Muscle - innervation Masseter Muscle - physiology Membrane Potentials - drug effects Mes-V nucleus Mesencephalon - cytology Mesencephalon - drug effects Mesencephalon - physiology Motor reflex NADPH Dehydrogenase - metabolism Nerve Fibers - drug effects Nerve Fibers - enzymology Neuromodulation Nitric oxide Nitric Oxide - pharmacology Nitric Oxide Donors - antagonists & inhibitors Nitric Oxide Donors - pharmacology Nitric Oxide Synthase - physiology Nitric Oxide Synthase Type I Polyamines - antagonists & inhibitors Polyamines - pharmacology Propioception Rats Rats, Wistar Sensory Trigeminal Trigeminal Nerve - drug effects Trigeminal Nerve - enzymology Trigeminal Nerve - physiology Vertebrates: nervous system and sense organs |
| title | Mesencephalic trigeminal neurons are innervated by nitric oxide synthase-containing fibers and respond to nitric oxide |
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