Retrotransposon expression and incorporation of cloned human and mouse retroelements in human spermatozoa

Objective To investigate the expression of long interspersed element (LINE) 1, human endogenous retrovirus (HERV) K10, and short interspersed element–VNTR–Alu element (SVA) retrotransposons in ejaculated human spermatozoa by means of reverse-transcription (RT) polymerase chain reaction (PCR) analysi...

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Veröffentlicht in:	Fertility and sterility 2017-03, Vol.107 (3), p.821-830
Hauptverfasser:	Lazaros, Leandros, Ph.D, Kitsou, Chrysoula, Ph.D, Kostoulas, Charilaos, B.Sc, Bellou, Sofia, Ph.D, Hatzi, Elissavet, Ph.D, Ladias, Paris, B.Sc, Stefos, Theodoros, Markoula, Sofia, Ph.D, Galani, Vasiliki, Ph.D, Vartholomatos, Georgios, Ph.D, Tzavaras, Theodore, Ph.D, Georgiou, Ioannis
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Separation - methods Cloning, Molecular Endopeptidases - biosynthesis Endopeptidases - genetics Flow Cytometry Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics HERV-K10 human spermatozoa Humans Internal Medicine LINE-1 Long Interspersed Nucleotide Elements Male Mice Microscopy, Confocal Minisatellite Repeats Obstetrics and Gynecology Oligospermia - genetics retroelements retrotransposition Reverse Transcriptase Polymerase Chain Reaction Spermatozoa - metabolism Transcription, Genetic Transfection
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