Full-term potential of goat in vitro produced embryos after different cryopreservation methods
Cryopreservation of preimplantation embryos represents a major challenge due to their shape and relatively large cells. Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aim...
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| Veröffentlicht in: | Cryobiology 2017-04, Vol.75, p.75-79 |
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| creator | Ferreira-Silva, José Carlos Moura, Marcelo Tigre Silva, Túlio Diego Oliveira, Luis Rennan Sampaio Chiamenti, Adauto Figueirêdo Freitas, Vicente José Oliveira, Marcos Antonio Lemos |
| description | Cryopreservation of preimplantation embryos represents a major challenge due to their shape and relatively large cells. Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aimed to compare different cryopreservation methods for goat in vitro produced (IVP) embryos. Goat blastocysts were subjected to conventional freezing (CF), Dimethyl sulfoxide vitrification (DMSO-V) and Dimethylformamide vitrification (DMF-V). Cryopreserved blastocysts were assessed for re-expansion, cell viability and in vivo development rates. Blastocyst re-expansion after cryopreservation was similar between groups, but cell viability was lower for DMF-V (32%) than CF (68%) and DMSO-V (60%). Pregnancy and delivery rates were similar for CF (60% and 50%) and DMSO-V (50% and 45%) and higher then DMF-V (20% and 15%), respectively. Finally, kidding rates were also indistinguishable for CF (40%) and DMSO-V (35%), but higher then DMF-V (12.5%). In conclusion, conventional freezing and vitrification using DMSO have similar efficiencies for cryopreservation of goat IVP embryos and cryoprotectant for vitrification affects its outcome. |
| doi_str_mv | 10.1016/j.cryobiol.2017.01.009 |
| format | Article |
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Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aimed to compare different cryopreservation methods for goat in vitro produced (IVP) embryos. Goat blastocysts were subjected to conventional freezing (CF), Dimethyl sulfoxide vitrification (DMSO-V) and Dimethylformamide vitrification (DMF-V). Cryopreserved blastocysts were assessed for re-expansion, cell viability and in vivo development rates. Blastocyst re-expansion after cryopreservation was similar between groups, but cell viability was lower for DMF-V (32%) than CF (68%) and DMSO-V (60%). Pregnancy and delivery rates were similar for CF (60% and 50%) and DMSO-V (50% and 45%) and higher then DMF-V (20% and 15%), respectively. Finally, kidding rates were also indistinguishable for CF (40%) and DMSO-V (35%), but higher then DMF-V (12.5%). In conclusion, conventional freezing and vitrification using DMSO have similar efficiencies for cryopreservation of goat IVP embryos and cryoprotectant for vitrification affects its outcome.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2017.01.009</identifier><identifier>PMID: 28137429</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Blastocyst - drug effects ; Capra hircus ; Caprine ; Cell Survival - drug effects ; Cryobanking ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Dimethyl Sulfoxide - pharmacology ; Dimethylformamide - pharmacology ; Embryo Transfer ; Embryo, Mammalian ; Fertilization in Vitro - methods ; Freezing ; Goats ; IVF ; Vitrification - drug effects</subject><ispartof>Cryobiology, 2017-04, Vol.75, p.75-79</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. 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Embryo source and cryopreservation method are key factors to cryotolerance efficiency and few reports have investigated more promising protocols for goat embryos. The study was aimed to compare different cryopreservation methods for goat in vitro produced (IVP) embryos. Goat blastocysts were subjected to conventional freezing (CF), Dimethyl sulfoxide vitrification (DMSO-V) and Dimethylformamide vitrification (DMF-V). Cryopreserved blastocysts were assessed for re-expansion, cell viability and in vivo development rates. Blastocyst re-expansion after cryopreservation was similar between groups, but cell viability was lower for DMF-V (32%) than CF (68%) and DMSO-V (60%). Pregnancy and delivery rates were similar for CF (60% and 50%) and DMSO-V (50% and 45%) and higher then DMF-V (20% and 15%), respectively. Finally, kidding rates were also indistinguishable for CF (40%) and DMSO-V (35%), but higher then DMF-V (12.5%). In conclusion, conventional freezing and vitrification using DMSO have similar efficiencies for cryopreservation of goat IVP embryos and cryoprotectant for vitrification affects its outcome.</description><subject>Animals</subject><subject>Blastocyst - drug effects</subject><subject>Capra hircus</subject><subject>Caprine</subject><subject>Cell Survival - drug effects</subject><subject>Cryobanking</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>Dimethylformamide - pharmacology</subject><subject>Embryo Transfer</subject><subject>Embryo, Mammalian</subject><subject>Fertilization in Vitro - methods</subject><subject>Freezing</subject><subject>Goats</subject><subject>IVF</subject><subject>Vitrification - drug effects</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtuGzEQRYkgRqzI-QWBpZvdDMnVProYRvwADLiJa4KPoUNhd6mQXAH-m3xLvswUZKd1Nc25c2cOIRsGNQPWft_VJr4E7cNYc2BdDawGGD6RFYMBKi4G_pmsABirOG_gnHxNaQcAbSeaL-Sc90x0DR9WRN4s41hljBPdh4xz9mqkwdHnoDL187-_B59joPsY7GLQUpx06U1UuZKh1juHsaTo8Zp9xITxoLIPM50w_w42XZAzp8aE397mmjzd_Px1fVc9PN7eX189VKZhba7QuKE3jvEtuF4rIewWnbaON8i3XLuOO2XEYK3usWdoB9FojsBdJ6DpQYs1uTztLZf-WTBlOflkcBzVjGFJkvWt4Gxou7ag7Qk1MaQU0cl99JOKL5KBPMqVO_kuVx7lSmCyyC3BzVvHoie0_2PvNgvw4wRg-fTgMcpkPM7Fm49osrTBf9TxCikiknI</recordid><startdate>201704</startdate><enddate>201704</enddate><creator>Ferreira-Silva, José Carlos</creator><creator>Moura, Marcelo Tigre</creator><creator>Silva, Túlio Diego</creator><creator>Oliveira, Luis Rennan Sampaio</creator><creator>Chiamenti, Adauto</creator><creator>Figueirêdo Freitas, Vicente José</creator><creator>Oliveira, Marcos Antonio Lemos</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201704</creationdate><title>Full-term potential of goat in vitro produced embryos after different cryopreservation methods</title><author>Ferreira-Silva, José Carlos ; 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| subjects | Animals Blastocyst - drug effects Capra hircus Caprine Cell Survival - drug effects Cryobanking Cryopreservation - methods Cryoprotective Agents - pharmacology Dimethyl Sulfoxide - pharmacology Dimethylformamide - pharmacology Embryo Transfer Embryo, Mammalian Fertilization in Vitro - methods Freezing Goats IVF Vitrification - drug effects |
| title | Full-term potential of goat in vitro produced embryos after different cryopreservation methods |
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