Development of Caco-2 cells co-expressing CYP3A4 and NADPH-cytochrome P450 reductase using a human artificial chromosome for the prediction of intestinal extraction ratio of CYP3A4 substrates

The Caco-2 cells co-expressing cytochrome P450 (CYP) 3A4 and NADPH-cytochrome P450 reductase (CPR) were developed using a human artificial chromosome (HAC) vector. The CYP3A4 and CPR genes were cloned into the HAC vector in CHO cells using the Cre-loxP system, and the microcell-mediated chromosome t...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Drug metabolism and pharmacokinetics 2017-02, Vol.32 (1), p.61-68
Hauptverfasser:	Takenaka, Toru, Kazuki, Kanako, Harada, Naomoto, Kuze, Jiro, Chiba, Masato, Iwao, Takahiro, Matsunaga, Tamihide, Abe, Satoshi, Oshimura, Mitsuo, Kazuki, Yasuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Caco-2 Cells Caco-2 cells Chromosomes, Artificial, Human - genetics CYP3A4 Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Human artificial chromosome vector Humans Intestinal metabolism Intestines - metabolism NADPH-cytochrome P450 reductase NADPH-Ferrihemoprotein Reductase - genetics NADPH-Ferrihemoprotein Reductase - metabolism Substrate Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!