Nucleoid clumping is dispensable for the Dps-dependent hydrogen peroxide resistance in Staphylococcus aureus
Dps family proteins have the ferroxidase activity that contributes to oxidative stress resistance. In addition, a part of Dps family proteins including Escherichia coli Dps and Staphylococcus aureus MrgA (metallo regulon gene A) bind DNA and induce the structural change of the nucleoid. We previousl...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2016-10, Vol.162 (10), p.1822-1828 |
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| container_title | Microbiology (Society for General Microbiology) |
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| creator | Ushijima, Yuri Yoshida, Osamu Villanueva, Montecillo Jake Adolf Ohniwa, Ryosuke L Morikawa, Kazuya |
| description | Dps family proteins have the ferroxidase activity that contributes to oxidative stress resistance. In addition, a part of Dps family proteins including Escherichia coli Dps and Staphylococcus aureus MrgA (metallo regulon gene A) bind DNA and induce the structural change of the nucleoid. We previously showed that a mutated MrgA with reduced ferroxidase activity was unable to contribute to the hydrogen peroxide (H2O2) and UV resistance in S. aureus, suggesting that the nucleoid clumping by MrgA is not sufficient for the resistance. However, it remained elusive whether the nucleoid clumping is dispensable for the resistance. Here, we aimed to clarify this question by employing the E. coli Dps lacking DNA-binding activity, DpsΔ18. Staphylococcal nucleoid was clumped by E. coli Dps, but not by DpsΔ18. H2O2 stress assay indicated that Dps and DpsΔ18 restored the reduced susceptibility of S. aureus ΔmrgA. Thus, we concluded that the staphylococcal nucleoid clumping is dispensable for the Dps-mediated H2O2 resistance. In contrast, Dps was unable to complement S. aureus ΔmrgA in the UV resistance, suggesting the MrgA function that cannot be compensated for by E. coli Dps. |
| doi_str_mv | 10.1099/mic.0.000353 |
| format | Article |
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In addition, a part of Dps family proteins including Escherichia coli Dps and Staphylococcus aureus MrgA (metallo regulon gene A) bind DNA and induce the structural change of the nucleoid. We previously showed that a mutated MrgA with reduced ferroxidase activity was unable to contribute to the hydrogen peroxide (H2O2) and UV resistance in S. aureus, suggesting that the nucleoid clumping by MrgA is not sufficient for the resistance. However, it remained elusive whether the nucleoid clumping is dispensable for the resistance. Here, we aimed to clarify this question by employing the E. coli Dps lacking DNA-binding activity, DpsΔ18. Staphylococcal nucleoid was clumped by E. coli Dps, but not by DpsΔ18. H2O2 stress assay indicated that Dps and DpsΔ18 restored the reduced susceptibility of S. aureus ΔmrgA. Thus, we concluded that the staphylococcal nucleoid clumping is dispensable for the Dps-mediated H2O2 resistance. 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In addition, a part of Dps family proteins including Escherichia coli Dps and Staphylococcus aureus MrgA (metallo regulon gene A) bind DNA and induce the structural change of the nucleoid. We previously showed that a mutated MrgA with reduced ferroxidase activity was unable to contribute to the hydrogen peroxide (H2O2) and UV resistance in S. aureus, suggesting that the nucleoid clumping by MrgA is not sufficient for the resistance. However, it remained elusive whether the nucleoid clumping is dispensable for the resistance. Here, we aimed to clarify this question by employing the E. coli Dps lacking DNA-binding activity, DpsΔ18. Staphylococcal nucleoid was clumped by E. coli Dps, but not by DpsΔ18. H2O2 stress assay indicated that Dps and DpsΔ18 restored the reduced susceptibility of S. aureus ΔmrgA. Thus, we concluded that the staphylococcal nucleoid clumping is dispensable for the Dps-mediated H2O2 resistance. In contrast, Dps was unable to complement S. aureus ΔmrgA in the UV resistance, suggesting the MrgA function that cannot be compensated for by E. coli Dps.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Drug Resistance, Bacterial</subject><subject>Escherichia coli</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Oxidative Stress</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTlP7EAQhEcIxJ0RowkJ8NJzrh0i3uOQEARAbM3RZgfZHjNjS-y_x2iBmKhb3V9VUEXICYMFg6q66IJbwAIAhBJbZJ9JrQoOJWzPu1BQQLnke-Qg5zeA-Qlsl-zxpRIVl2yftA-TazEGT107dUPoX2nI1Ic8YJ-NbZE2MdFxhfTfkAuP89ljP9LV2qf4ij0dMMWP4JEmzCGPpndIQ0-fRjOs1m100bkpUzMlnPIR2WlMm_H4ex6Sl-v_z1e3xf3jzd3V5X3hJC_HQjMrpBHSo0HTSNMIYY0VTaksSuF5uVTcmEbZximhrV5WRhsNUFkN3CMXh-Rs4zuk-D5hHusuZIdta3qMU65ZqQVnDMq_oFJxJTXXM3q-QV2KOSds6iGFzqR1zaD-qmKWuhrqTRUzfvrtPNkO_S_8k734BOZhhpY</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Ushijima, Yuri</creator><creator>Yoshida, Osamu</creator><creator>Villanueva, Montecillo Jake Adolf</creator><creator>Ohniwa, Ryosuke L</creator><creator>Morikawa, Kazuya</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201610</creationdate><title>Nucleoid clumping is dispensable for the Dps-dependent hydrogen peroxide resistance in Staphylococcus aureus</title><author>Ushijima, Yuri ; Yoshida, Osamu ; Villanueva, Montecillo Jake Adolf ; Ohniwa, Ryosuke L ; Morikawa, Kazuya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-61b34a34deaeaf4af33bab3f85be43d28752aaf5bfc536b679a6a6009b602de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Drug Resistance, Bacterial</topic><topic>Escherichia coli</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Oxidative Stress</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ushijima, Yuri</creatorcontrib><creatorcontrib>Yoshida, Osamu</creatorcontrib><creatorcontrib>Villanueva, Montecillo Jake Adolf</creatorcontrib><creatorcontrib>Ohniwa, Ryosuke L</creatorcontrib><creatorcontrib>Morikawa, Kazuya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ushijima, Yuri</au><au>Yoshida, Osamu</au><au>Villanueva, Montecillo Jake Adolf</au><au>Ohniwa, Ryosuke L</au><au>Morikawa, Kazuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleoid clumping is dispensable for the Dps-dependent hydrogen peroxide resistance in Staphylococcus aureus</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2016-10</date><risdate>2016</risdate><volume>162</volume><issue>10</issue><spage>1822</spage><epage>1828</epage><pages>1822-1828</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Dps family proteins have the ferroxidase activity that contributes to oxidative stress resistance. In addition, a part of Dps family proteins including Escherichia coli Dps and Staphylococcus aureus MrgA (metallo regulon gene A) bind DNA and induce the structural change of the nucleoid. We previously showed that a mutated MrgA with reduced ferroxidase activity was unable to contribute to the hydrogen peroxide (H2O2) and UV resistance in S. aureus, suggesting that the nucleoid clumping by MrgA is not sufficient for the resistance. However, it remained elusive whether the nucleoid clumping is dispensable for the resistance. Here, we aimed to clarify this question by employing the E. coli Dps lacking DNA-binding activity, DpsΔ18. Staphylococcal nucleoid was clumped by E. coli Dps, but not by DpsΔ18. H2O2 stress assay indicated that Dps and DpsΔ18 restored the reduced susceptibility of S. aureus ΔmrgA. Thus, we concluded that the staphylococcal nucleoid clumping is dispensable for the Dps-mediated H2O2 resistance. In contrast, Dps was unable to complement S. aureus ΔmrgA in the UV resistance, suggesting the MrgA function that cannot be compensated for by E. coli Dps.</abstract><cop>England</cop><pmid>27539241</pmid><doi>10.1099/mic.0.000353</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; PubMed Central |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Cell Nucleus - genetics Cell Nucleus - metabolism Drug Resistance, Bacterial Escherichia coli Gene Expression Regulation, Bacterial Hydrogen Peroxide - pharmacology Oxidative Stress Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - genetics Staphylococcus aureus - metabolism |
| title | Nucleoid clumping is dispensable for the Dps-dependent hydrogen peroxide resistance in Staphylococcus aureus |
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