Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds
Abstract Introduction This study aimed to evaluate the efficacy of epigallocatechin gallate (EGCG), an antibacterial cross-linking agent, on the proliferation and differentiation of human dental pulp cells (hDPCs) cultured in hydrogel collagen scaffolds. Methods The odontogenic differentiation induc...
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| Veröffentlicht in: | Journal of endodontics 2017-02, Vol.43 (2), p.289-296 |
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| creator | Kwon, Young-Sun, MS Kim, Hee-Jin, DDS, MSD Hwang, Yun-Chan, DDS, PhD Rosa, Vinicius, DDS, PhD Yu, Mi-Kyung, DDS, PhD Min, Kyung-San, DDS, PhD |
| description | Abstract Introduction This study aimed to evaluate the efficacy of epigallocatechin gallate (EGCG), an antibacterial cross-linking agent, on the proliferation and differentiation of human dental pulp cells (hDPCs) cultured in hydrogel collagen scaffolds. Methods The odontogenic differentiation induced by EGCG was evaluated by alkaline phosphatase (ALP) activity and odontogenic-related gene expression using real-time polymerase chain reaction. The antibacterial effect of EGCG was investigated by a disc diffusion assay in comparison with glutaraldehyde. Proliferation was analyzed by cell number counting under both optical and confocal laser scanning microscopes. To assess the mechanical properties of collagen treated with EGCG, the setting time, surface roughness, and compressive strength were measured. Results EGCG itself did not up-regulate the odontogenic-related markers ( P > .05) although ALP activity was slightly increased. The proliferation and differentiation of hDPCs cultured in collagen increased significantly in the presence of EGCG ( P |
| doi_str_mv | 10.1016/j.joen.2016.10.017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1862939875</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0099239916307440</els_id><sourcerecordid>1862939875</sourcerecordid><originalsourceid>FETCH-LOGICAL-c477t-1a0983c9e8bcc4c16f6461b73541181aa7a7855067e663fd5f10529cd3e1f39c3</originalsourceid><addsrcrecordid>eNp9UsFu1DAQtRCIbhd-gAPykUOz2HESJxJCWqXbFqkSlQpny-uMF2-99mInlfpJ_CUTbeHAgZNnxm_es-cNIe84W3HGm4_71T5CWJUYY2HFuHxBFryVbSHqunpJFox1XVGKrjsj5znvGSKEkK_JWdlyUUouFuTXxlowY6bR0s3R7bT30egRzA8X6DVmGF9QHeg6jG6rzQjJaU_7FHMuvAsPLuzoegdhvKAx0LsUvbOQ9Ogw02Gglw4FEt67Uw11bqYDEl5iDZnuJn-kPXifaT_5cUowUJTuI0ojLb032troh_yGvLLaZ3j7fC7J96vNt_6muP16_aVf3xamknIsuGZdK0wH7daYyvDGNlXDt1LUFect11pq2dY1ayQ0jbBDbTmry84MArgVnRFL8uHEe0zx5wR5VAeXDT5QB4hTVrxtyk50rawRWp6gZh5HAquOyR10elKcqdkitVezRWq2aK7NBizJ-2f-aXuA4W_LH08Q8OkEAPzlo4OksnEQDAwuoVVqiO7__J__aTfokzPaP8AT5H2cUsD5Ka5yqZi6n5dk3hHeCCarionfD2W5Gg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1862939875</pqid></control><display><type>article</type><title>Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Kwon, Young-Sun, MS ; Kim, Hee-Jin, DDS, MSD ; Hwang, Yun-Chan, DDS, PhD ; Rosa, Vinicius, DDS, PhD ; Yu, Mi-Kyung, DDS, PhD ; Min, Kyung-San, DDS, PhD</creator><creatorcontrib>Kwon, Young-Sun, MS ; Kim, Hee-Jin, DDS, MSD ; Hwang, Yun-Chan, DDS, PhD ; Rosa, Vinicius, DDS, PhD ; Yu, Mi-Kyung, DDS, PhD ; Min, Kyung-San, DDS, PhD</creatorcontrib><description>Abstract Introduction This study aimed to evaluate the efficacy of epigallocatechin gallate (EGCG), an antibacterial cross-linking agent, on the proliferation and differentiation of human dental pulp cells (hDPCs) cultured in hydrogel collagen scaffolds. Methods The odontogenic differentiation induced by EGCG was evaluated by alkaline phosphatase (ALP) activity and odontogenic-related gene expression using real-time polymerase chain reaction. The antibacterial effect of EGCG was investigated by a disc diffusion assay in comparison with glutaraldehyde. Proliferation was analyzed by cell number counting under both optical and confocal laser scanning microscopes. To assess the mechanical properties of collagen treated with EGCG, the setting time, surface roughness, and compressive strength were measured. Results EGCG itself did not up-regulate the odontogenic-related markers ( P > .05) although ALP activity was slightly increased. The proliferation and differentiation of hDPCs cultured in collagen increased significantly in the presence of EGCG ( P < .05). The antibacterial activity of EGCG was similar to that of glutaraldehyde. The setting time of collagen was significantly shortened when it was treated with EGCG ( P < .05). The surface roughness and compressive strength of the cross-linked collagen were higher than those of collagen without EGCG ( P < .05). Conclusions Our results showed that EGCG, the antibacterial cross-linking agent, promoted the proliferation and differentiation of hDPCs cultured in collagen scaffolds. Furthermore, the enhanced mechanical properties of collagen scaffolds induced by EGCG may play important roles in cell behavior. Consequently, the application of EGCG to collagen scaffolds might be beneficial for regenerative endodontic therapy.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2016.10.017</identifier><identifier>PMID: 28132713</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alkaline Phosphatase - metabolism ; Anti-Bacterial Agents - pharmacology ; Antibacterial ; Catechin - analogs & derivatives ; Catechin - pharmacology ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Collagen ; cross-linking ; Cross-Linking Reagents - pharmacology ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental Pulp - growth & development ; Dentistry ; Endocrinology & Metabolism ; epigallocatechin gallate ; Gene Expression Profiling ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; odontogenic differentiation ; proliferation ; Real-Time Polymerase Chain Reaction ; Tissue Scaffolds</subject><ispartof>Journal of endodontics, 2017-02, Vol.43 (2), p.289-296</ispartof><rights>American Association of Endodontists</rights><rights>2016 American Association of Endodontists</rights><rights>Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-1a0983c9e8bcc4c16f6461b73541181aa7a7855067e663fd5f10529cd3e1f39c3</citedby><cites>FETCH-LOGICAL-c477t-1a0983c9e8bcc4c16f6461b73541181aa7a7855067e663fd5f10529cd3e1f39c3</cites><orcidid>0000-0002-7891-9565 ; 0000-0002-9203-7657 ; 0000-0002-1928-3384</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0099239916307440$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28132713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwon, Young-Sun, MS</creatorcontrib><creatorcontrib>Kim, Hee-Jin, DDS, MSD</creatorcontrib><creatorcontrib>Hwang, Yun-Chan, DDS, PhD</creatorcontrib><creatorcontrib>Rosa, Vinicius, DDS, PhD</creatorcontrib><creatorcontrib>Yu, Mi-Kyung, DDS, PhD</creatorcontrib><creatorcontrib>Min, Kyung-San, DDS, PhD</creatorcontrib><title>Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction This study aimed to evaluate the efficacy of epigallocatechin gallate (EGCG), an antibacterial cross-linking agent, on the proliferation and differentiation of human dental pulp cells (hDPCs) cultured in hydrogel collagen scaffolds. Methods The odontogenic differentiation induced by EGCG was evaluated by alkaline phosphatase (ALP) activity and odontogenic-related gene expression using real-time polymerase chain reaction. The antibacterial effect of EGCG was investigated by a disc diffusion assay in comparison with glutaraldehyde. Proliferation was analyzed by cell number counting under both optical and confocal laser scanning microscopes. To assess the mechanical properties of collagen treated with EGCG, the setting time, surface roughness, and compressive strength were measured. Results EGCG itself did not up-regulate the odontogenic-related markers ( P > .05) although ALP activity was slightly increased. The proliferation and differentiation of hDPCs cultured in collagen increased significantly in the presence of EGCG ( P < .05). The antibacterial activity of EGCG was similar to that of glutaraldehyde. The setting time of collagen was significantly shortened when it was treated with EGCG ( P < .05). The surface roughness and compressive strength of the cross-linked collagen were higher than those of collagen without EGCG ( P < .05). Conclusions Our results showed that EGCG, the antibacterial cross-linking agent, promoted the proliferation and differentiation of hDPCs cultured in collagen scaffolds. Furthermore, the enhanced mechanical properties of collagen scaffolds induced by EGCG may play important roles in cell behavior. Consequently, the application of EGCG to collagen scaffolds might be beneficial for regenerative endodontic therapy.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>cross-linking</subject><subject>Cross-Linking Reagents - pharmacology</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental Pulp - growth & development</subject><subject>Dentistry</subject><subject>Endocrinology & Metabolism</subject><subject>epigallocatechin gallate</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate</subject><subject>odontogenic differentiation</subject><subject>proliferation</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Tissue Scaffolds</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UsFu1DAQtRCIbhd-gAPykUOz2HESJxJCWqXbFqkSlQpny-uMF2-99mInlfpJ_CUTbeHAgZNnxm_es-cNIe84W3HGm4_71T5CWJUYY2HFuHxBFryVbSHqunpJFox1XVGKrjsj5znvGSKEkK_JWdlyUUouFuTXxlowY6bR0s3R7bT30egRzA8X6DVmGF9QHeg6jG6rzQjJaU_7FHMuvAsPLuzoegdhvKAx0LsUvbOQ9Ogw02Gglw4FEt67Uw11bqYDEl5iDZnuJn-kPXifaT_5cUowUJTuI0ojLb032troh_yGvLLaZ3j7fC7J96vNt_6muP16_aVf3xamknIsuGZdK0wH7daYyvDGNlXDt1LUFect11pq2dY1ayQ0jbBDbTmry84MArgVnRFL8uHEe0zx5wR5VAeXDT5QB4hTVrxtyk50rawRWp6gZh5HAquOyR10elKcqdkitVezRWq2aK7NBizJ-2f-aXuA4W_LH08Q8OkEAPzlo4OksnEQDAwuoVVqiO7__J__aTfokzPaP8AT5H2cUsD5Ka5yqZi6n5dk3hHeCCarionfD2W5Gg</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Kwon, Young-Sun, MS</creator><creator>Kim, Hee-Jin, DDS, MSD</creator><creator>Hwang, Yun-Chan, DDS, PhD</creator><creator>Rosa, Vinicius, DDS, PhD</creator><creator>Yu, Mi-Kyung, DDS, PhD</creator><creator>Min, Kyung-San, DDS, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7891-9565</orcidid><orcidid>https://orcid.org/0000-0002-9203-7657</orcidid><orcidid>https://orcid.org/0000-0002-1928-3384</orcidid></search><sort><creationdate>20170201</creationdate><title>Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds</title><author>Kwon, Young-Sun, MS ; Kim, Hee-Jin, DDS, MSD ; Hwang, Yun-Chan, DDS, PhD ; Rosa, Vinicius, DDS, PhD ; Yu, Mi-Kyung, DDS, PhD ; Min, Kyung-San, DDS, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-1a0983c9e8bcc4c16f6461b73541181aa7a7855067e663fd5f10529cd3e1f39c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>cross-linking</topic><topic>Cross-Linking Reagents - pharmacology</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dental Pulp - growth & development</topic><topic>Dentistry</topic><topic>Endocrinology & Metabolism</topic><topic>epigallocatechin gallate</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate</topic><topic>odontogenic differentiation</topic><topic>proliferation</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwon, Young-Sun, MS</creatorcontrib><creatorcontrib>Kim, Hee-Jin, DDS, MSD</creatorcontrib><creatorcontrib>Hwang, Yun-Chan, DDS, PhD</creatorcontrib><creatorcontrib>Rosa, Vinicius, DDS, PhD</creatorcontrib><creatorcontrib>Yu, Mi-Kyung, DDS, PhD</creatorcontrib><creatorcontrib>Min, Kyung-San, DDS, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwon, Young-Sun, MS</au><au>Kim, Hee-Jin, DDS, MSD</au><au>Hwang, Yun-Chan, DDS, PhD</au><au>Rosa, Vinicius, DDS, PhD</au><au>Yu, Mi-Kyung, DDS, PhD</au><au>Min, Kyung-San, DDS, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>43</volume><issue>2</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction This study aimed to evaluate the efficacy of epigallocatechin gallate (EGCG), an antibacterial cross-linking agent, on the proliferation and differentiation of human dental pulp cells (hDPCs) cultured in hydrogel collagen scaffolds. Methods The odontogenic differentiation induced by EGCG was evaluated by alkaline phosphatase (ALP) activity and odontogenic-related gene expression using real-time polymerase chain reaction. The antibacterial effect of EGCG was investigated by a disc diffusion assay in comparison with glutaraldehyde. Proliferation was analyzed by cell number counting under both optical and confocal laser scanning microscopes. To assess the mechanical properties of collagen treated with EGCG, the setting time, surface roughness, and compressive strength were measured. Results EGCG itself did not up-regulate the odontogenic-related markers ( P > .05) although ALP activity was slightly increased. The proliferation and differentiation of hDPCs cultured in collagen increased significantly in the presence of EGCG ( P < .05). The antibacterial activity of EGCG was similar to that of glutaraldehyde. The setting time of collagen was significantly shortened when it was treated with EGCG ( P < .05). The surface roughness and compressive strength of the cross-linked collagen were higher than those of collagen without EGCG ( P < .05). Conclusions Our results showed that EGCG, the antibacterial cross-linking agent, promoted the proliferation and differentiation of hDPCs cultured in collagen scaffolds. Furthermore, the enhanced mechanical properties of collagen scaffolds induced by EGCG may play important roles in cell behavior. Consequently, the application of EGCG to collagen scaffolds might be beneficial for regenerative endodontic therapy.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28132713</pmid><doi>10.1016/j.joen.2016.10.017</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-7891-9565</orcidid><orcidid>https://orcid.org/0000-0002-9203-7657</orcidid><orcidid>https://orcid.org/0000-0002-1928-3384</orcidid></addata></record> |
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| subjects | Alkaline Phosphatase - metabolism Anti-Bacterial Agents - pharmacology Antibacterial Catechin - analogs & derivatives Catechin - pharmacology Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Collagen cross-linking Cross-Linking Reagents - pharmacology Dental Pulp - cytology Dental Pulp - drug effects Dental Pulp - growth & development Dentistry Endocrinology & Metabolism epigallocatechin gallate Gene Expression Profiling Humans Hydrogel, Polyethylene Glycol Dimethacrylate odontogenic differentiation proliferation Real-Time Polymerase Chain Reaction Tissue Scaffolds |
| title | Effects of Epigallocatechin Gallate, an Antibacterial Cross-linking Agent, on Proliferation and Differentiation of Human Dental Pulp Cells Cultured in Collagen Scaffolds |
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