Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hübner)
Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA en...
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| Veröffentlicht in: | Archives of insect biochemistry and physiology 2002-11, Vol.51 (3), p.103-110 |
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| container_title | Archives of insect biochemistry and physiology |
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| creator | Ren, Xiaoxia Han, Zhaojun Wang, Yinchang |
| description | Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5′ untranslated regions (UTR) of 315 bp and 3′ UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos. Arch. Insect Biochem. Physiol. 51:103–110, 2002. © 2002 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/arch.10054 |
| format | Article |
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Insect Biochem. Physiol</addtitle><description>Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5′ untranslated regions (UTR) of 315 bp and 3′ UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos. Arch. Insect Biochem. Physiol. 51:103–110, 2002. © 2002 Wiley‐Liss, Inc.</description><subject>acetylcholinesterase</subject><subject>Acetylcholinesterase - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cholinesterase Inhibitors</subject><subject>cotton bollworm</subject><subject>DNA, Complementary</subject><subject>gene mutation</subject><subject>insecticide resistance</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides</subject><subject>Molecular Sequence Data</subject><subject>Monocrotophos</subject><subject>Moths - enzymology</subject><subject>Moths - genetics</subject><subject>Sequence Homology, Amino Acid</subject><issn>0739-4462</issn><issn>1520-6327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQQC0EgrJc-ADkEwJEwEtip0dUloLKKhCCi2W7ExpI4mKnLP_GjR8jpQVunGYOb540D6FVSnYoIWxXezsYb0k8g1o0YSQSnMlZ1CKSt6M4FmwBLYbwSAhpC5rOowXKeCpSnrbQ3SnYga7yUAbsMly6ylnvajccuIA9hDzUurKA8wpbV9euwsYVxavz5TbuQpFb9wJ-qLH2Zf4AXuON7ueHqcBvLqO5TBcBVqZzCd0cHlx3ulHv_Oi4s9eLbCxIHGUEaNvEcWwogAFDhRGQpqzPqAbOoS-JASGTjFvKGbWGtI2VMiFM8L5gmi-h9Yl36N3zCEKtyjxYKApdgRsFRVPBUkp4A25NwObBEDxkaujzUvt3RYkah1TjkOo7ZAOvTa0jU0L_D52WawA6AV7zAt7_Uam9q073RxpNbpqq8PZ7o_2TEpLLRN2eHSnCLu8vevJE7fMvk-COYw</recordid><startdate>200211</startdate><enddate>200211</enddate><creator>Ren, Xiaoxia</creator><creator>Han, Zhaojun</creator><creator>Wang, Yinchang</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope></search><sort><creationdate>200211</creationdate><title>Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hübner)</title><author>Ren, Xiaoxia ; Han, Zhaojun ; Wang, Yinchang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4604-f0e19b444b1eebeb16b6e882d21ae33ed70be675f3c1321cb09bc7750263d62a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>acetylcholinesterase</topic><topic>Acetylcholinesterase - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cholinesterase Inhibitors</topic><topic>cotton bollworm</topic><topic>DNA, Complementary</topic><topic>gene mutation</topic><topic>insecticide resistance</topic><topic>Insecticide Resistance - genetics</topic><topic>Insecticides</topic><topic>Molecular Sequence Data</topic><topic>Monocrotophos</topic><topic>Moths - enzymology</topic><topic>Moths - genetics</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Xiaoxia</creatorcontrib><creatorcontrib>Han, Zhaojun</creatorcontrib><creatorcontrib>Wang, Yinchang</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Archives of insect biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Xiaoxia</au><au>Han, Zhaojun</au><au>Wang, Yinchang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hübner)</atitle><jtitle>Archives of insect biochemistry and physiology</jtitle><addtitle>Arch. Insect Biochem. Physiol</addtitle><date>2002-11</date><risdate>2002</risdate><volume>51</volume><issue>3</issue><spage>103</spage><epage>110</epage><pages>103-110</pages><issn>0739-4462</issn><eissn>1520-6327</eissn><abstract>Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5′ untranslated regions (UTR) of 315 bp and 3′ UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos. Arch. Insect Biochem. Physiol. 51:103–110, 2002. © 2002 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>12386838</pmid><doi>10.1002/arch.10054</doi><tpages>8</tpages></addata></record> |
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| subjects | acetylcholinesterase Acetylcholinesterase - genetics Amino Acid Sequence Animals Base Sequence Cholinesterase Inhibitors cotton bollworm DNA, Complementary gene mutation insecticide resistance Insecticide Resistance - genetics Insecticides Molecular Sequence Data Monocrotophos Moths - enzymology Moths - genetics Sequence Homology, Amino Acid |
| title | Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hübner) |
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