Cell‐controlled hybrid perfusion fed‐batch CHO cell process provides significant productivity improvement over conventional fed‐batch cultures

ABSTRACT A simple method originally designed to control lactate accumulation in fed‐batch cultures of Chinese Hamster Ovary (CHO) cells has been modified and extended to allow cells in culture to control their own rate of perfusion to precisely deliver nutritional requirements. The method allows for...

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Veröffentlicht in:	Biotechnology and bioengineering 2017-07, Vol.114 (7), p.1438-1447
Hauptverfasser:	Hiller, Gregory W., Ovalle, Ana Maria, Gagnon, Matthew P., Curran, Meredith L., Wang, Wenge
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Batch Cell Culture Techniques - instrumentation Batch Cell Culture Techniques - methods Batch culture Bioreactors Biotechnology Cell culture Cell Proliferation - physiology CHO cell culture CHO Cells - cytology CHO Cells - physiology Cricetulus Fed batch Fed-batch culture Flocculation Glucose - metabolism glucose limitation HIPCOP HIPDOG hybrid perfusion fed‐batch Lactic acid Lactic Acid - metabolism mammalian cell culture Monoclonal antibodies Nutritional requirements Perfusion Perfusion - methods Productivity
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