The Extracellular Proteases Secreted in vitro and in planta by the Phytopathogenic Fungus Sclerotinia sclerotiorum
The proteolytic enzymes secreted by Sclerotinia sclerotiorum were characterized using mechanistic inhibitors, affinity chromatography and analytical electrophoresis. The proteolytic system mainly constituted of acidic proteases but a neutral protease (serine protease) was also found at the initial s...
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| Veröffentlicht in: | Journal of phytopathology 2002-09, Vol.150 (8-9), p.507-511 |
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| container_issue | 8-9 |
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| container_title | Journal of phytopathology |
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| creator | Billon-Grand, G. Poussereau, N. Fèvre, M. |
| description | The proteolytic enzymes secreted by Sclerotinia sclerotiorum were characterized using mechanistic inhibitors, affinity chromatography and analytical electrophoresis. The proteolytic system mainly constituted of acidic proteases but a neutral protease (serine protease) was also found at the initial stage of plant infection by the fungus. In vitro and in planta protease profiles were shown to be similar. Properties of the enzymes produced were matched with the ambient pH indicating that the set of enzymes secreted reflected the environmental niche created during mycelial growth. |
| doi_str_mv | 10.1046/j.1439-0434.2002.00782.x |
| format | Article |
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The proteolytic system mainly constituted of acidic proteases but a neutral protease (serine protease) was also found at the initial stage of plant infection by the fungus. In vitro and in planta protease profiles were shown to be similar. Properties of the enzymes produced were matched with the ambient pH indicating that the set of enzymes secreted reflected the environmental niche created during mycelial growth.</description><identifier>ISSN: 0931-1785</identifier><identifier>EISSN: 1439-0434</identifier><identifier>DOI: 10.1046/j.1439-0434.2002.00782.x</identifier><identifier>CODEN: JPHYEB</identifier><language>eng</language><publisher>Berlin, Germany: Blackwell Verlag GmbH</publisher><subject>Bacterial plant pathogens ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; fungal proteases ; pH regulation ; Phytopathology. Animal pests. 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The proteolytic system mainly constituted of acidic proteases but a neutral protease (serine protease) was also found at the initial stage of plant infection by the fungus. In vitro and in planta protease profiles were shown to be similar. Properties of the enzymes produced were matched with the ambient pH indicating that the set of enzymes secreted reflected the environmental niche created during mycelial growth.</description><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>fungal proteases</subject><subject>pH regulation</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>plant pathogenesis</subject><subject>Sclerotinia sclerotiorum</subject><subject>Systematics. Structure, properties and multiplication. Genetics</subject><subject>Variation, races, biotypes, parasitic specialization, genetics</subject><issn>0931-1785</issn><issn>1439-0434</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqNkF1v0zAUhiPEpJXBf_AN3CX4K44tcYOqfTBNo9I2dmmdmpPVJU2K7UD77-es1bjlysfy-7xHfoqCMFoxKtXndcWkMCWVQlacUl5R2mhe7d4Us9eHt8WMGsFK1uj6tHgX4zoHqaB0VoT7FZLzXQrgsOvGDgJZhCEhRIzkDl3AhD-J78kfn8JAoH-5bDvoE5DlnqSML1b7NGwhrYYn7L0jF2P_NGbadZirfO-BxOM8hHHzvjhpoYv44XieFQ8X5_fzq_Lm--W3-deb0klqeMm4ZGbJHGtrqYzTRsgaFDRiaRrgwJVQjTDKoEYmnDMKlWyNY42kWmJtxFnx6dC7DcPvEWOyGx-nX0KPwxgt04oLTUUO6kPQhSHGgK3dBr-BsLeM2kmyXdvJpZ1c2kmyfZFsdxn9eNwB0UHXBuidj_94YXgjmM65L4fcX9_h_r_77fXiKg8ZLw-4jwl3rziEXzZLaGr7eHtp7_SPW9mwuTXiGYEMny4</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Billon-Grand, G.</creator><creator>Poussereau, N.</creator><creator>Fèvre, M.</creator><general>Blackwell Verlag GmbH</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200209</creationdate><title>The Extracellular Proteases Secreted in vitro and in planta by the Phytopathogenic Fungus Sclerotinia sclerotiorum</title><author>Billon-Grand, G. ; Poussereau, N. ; Fèvre, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4092-12419b1c1f5469c89345a6a73b97a2a263673969e8e13cc96e64f9c174084e593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>fungal proteases</topic><topic>pH regulation</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>plant pathogenesis</topic><topic>Sclerotinia sclerotiorum</topic><topic>Systematics. Structure, properties and multiplication. Genetics</topic><topic>Variation, races, biotypes, parasitic specialization, genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Billon-Grand, G.</creatorcontrib><creatorcontrib>Poussereau, N.</creatorcontrib><creatorcontrib>Fèvre, M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Billon-Grand, G.</au><au>Poussereau, N.</au><au>Fèvre, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Extracellular Proteases Secreted in vitro and in planta by the Phytopathogenic Fungus Sclerotinia sclerotiorum</atitle><jtitle>Journal of phytopathology</jtitle><date>2002-09</date><risdate>2002</risdate><volume>150</volume><issue>8-9</issue><spage>507</spage><epage>511</epage><pages>507-511</pages><issn>0931-1785</issn><eissn>1439-0434</eissn><coden>JPHYEB</coden><abstract>The proteolytic enzymes secreted by Sclerotinia sclerotiorum were characterized using mechanistic inhibitors, affinity chromatography and analytical electrophoresis. 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| ispartof | Journal of phytopathology, 2002-09, Vol.150 (8-9), p.507-511 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Bacterial plant pathogens Biological and medical sciences Fundamental and applied biological sciences. Psychology Fungal plant pathogens fungal proteases pH regulation Phytopathology. Animal pests. Plant and forest protection plant pathogenesis Sclerotinia sclerotiorum Systematics. Structure, properties and multiplication. Genetics Variation, races, biotypes, parasitic specialization, genetics |
| title | The Extracellular Proteases Secreted in vitro and in planta by the Phytopathogenic Fungus Sclerotinia sclerotiorum |
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