Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro
Contents We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and there...
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| Veröffentlicht in: | Reproduction in domestic animals 2017-06, Vol.52 (3), p.409-421 |
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| creator | Ambrogi, M Dall'Acqua, PC Rocha‐Frigoni, NAS Leão, BCS Mingoti, GZ |
| description | Contents
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p |
| doi_str_mv | 10.1111/rda.12923 |
| format | Article |
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We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p < .05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p < .05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non‐transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p < .05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p < .05). The blastocyst formation rates (36.9% average) and the re‐expansion rates of vitrified‐warmed blastocysts (53%, average) were unaffected (p > .05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12923</identifier><identifier>PMID: 28120355</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Antioxidants ; Antioxidants - pharmacology ; Blastocysts ; Bovine serum albumin ; Catalase ; Catalase - pharmacology ; Cattle ; Cold tolerance ; Culture Media ; Cysteamine ; Cysteamine - pharmacology ; Cysteine ; Cysteine - pharmacology ; Cytoplasm - physiology ; Embryo Culture Techniques - veterinary ; Embryogenesis ; Embryonic growth stage ; Embryos ; In Vitro Oocyte Maturation Techniques - methods ; In Vitro Oocyte Maturation Techniques - veterinary ; In vitro testing ; Macromolecules ; Maturation ; Membrane Potential, Mitochondrial ; Mitochondria ; Oocytes ; Reactive Oxygen Species ; Serum albumin ; Transport</subject><ispartof>Reproduction in domestic animals, 2017-06, Vol.52 (3), p.409-421</ispartof><rights>2017 Blackwell Verlag GmbH</rights><rights>2017 Blackwell Verlag GmbH.</rights><rights>Copyright © 2017 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-bafc34e970aab4a0f7e588c9a06032b012fe06e162ac7daf3dc80bf1bc27cd283</citedby><cites>FETCH-LOGICAL-c3533-bafc34e970aab4a0f7e588c9a06032b012fe06e162ac7daf3dc80bf1bc27cd283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12923$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12923$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28120355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ambrogi, M</creatorcontrib><creatorcontrib>Dall'Acqua, PC</creatorcontrib><creatorcontrib>Rocha‐Frigoni, NAS</creatorcontrib><creatorcontrib>Leão, BCS</creatorcontrib><creatorcontrib>Mingoti, GZ</creatorcontrib><title>Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p < .05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p < .05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non‐transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p < .05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p < .05). The blastocyst formation rates (36.9% average) and the re‐expansion rates of vitrified‐warmed blastocysts (53%, average) were unaffected (p > .05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Blastocysts</subject><subject>Bovine serum albumin</subject><subject>Catalase</subject><subject>Catalase - pharmacology</subject><subject>Cattle</subject><subject>Cold tolerance</subject><subject>Culture Media</subject><subject>Cysteamine</subject><subject>Cysteamine - pharmacology</subject><subject>Cysteine</subject><subject>Cysteine - pharmacology</subject><subject>Cytoplasm - physiology</subject><subject>Embryo Culture Techniques - veterinary</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>In Vitro Oocyte Maturation Techniques - methods</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>In vitro testing</subject><subject>Macromolecules</subject><subject>Maturation</subject><subject>Membrane Potential, Mitochondrial</subject><subject>Mitochondria</subject><subject>Oocytes</subject><subject>Reactive Oxygen Species</subject><subject>Serum albumin</subject><subject>Transport</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1rVDEQhoModl298A9IwJv24rT52PPlXan1AwqC1OtDTjLRlJzkmORs3R_m_-vsbvVCMCQMzDzzzpCXkNecnXM8F8mocy56IZ-QFd_IvmK15E_JivWyqZq26U7Ii5zvGON117bPyYnouGCyrlfk921SIc8xFRe-0zFuXQAao94VyNQFqugExi0Tzcs8e5ggFDD03pUf1DhrIWGCTkqnOEUPevHYpoLBV1z85QzG_I5eI6lLpjHQsGgPKh0gnBJnr_LkNGqUJSlsCocSTGPaxYAFA1vwcd5P3i-0dSXFl-SZVT7Dq8e4Jt8-XN9efapuvnz8fHV5U2lZS1mNymq5gb5lSo0bxWwLddfpXrGGSTEyLiywBngjlG6NstLojo2Wj1q02ohOrsnpUXdO8ecCuQyTyxq8VwHikgfeNXg7XteIvv0HvYtLCrgdUn3NeS9wpTU5O1L4YTknsMOc3KTSbuBs2Hs5oJfDwUtk3zwqLiOa8Jf8Yx4CF0fg3nnY_V9p-Pr-8ij5AOY7rhw</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Ambrogi, M</creator><creator>Dall'Acqua, PC</creator><creator>Rocha‐Frigoni, NAS</creator><creator>Leão, BCS</creator><creator>Mingoti, GZ</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201706</creationdate><title>Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro</title><author>Ambrogi, M ; Dall'Acqua, PC ; Rocha‐Frigoni, NAS ; Leão, BCS ; Mingoti, GZ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-bafc34e970aab4a0f7e588c9a06032b012fe06e162ac7daf3dc80bf1bc27cd283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Blastocysts</topic><topic>Bovine serum albumin</topic><topic>Catalase</topic><topic>Catalase - pharmacology</topic><topic>Cattle</topic><topic>Cold tolerance</topic><topic>Culture Media</topic><topic>Cysteamine</topic><topic>Cysteamine - pharmacology</topic><topic>Cysteine</topic><topic>Cysteine - pharmacology</topic><topic>Cytoplasm - physiology</topic><topic>Embryo Culture Techniques - veterinary</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>In Vitro Oocyte Maturation Techniques - methods</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>In vitro testing</topic><topic>Macromolecules</topic><topic>Maturation</topic><topic>Membrane Potential, Mitochondrial</topic><topic>Mitochondria</topic><topic>Oocytes</topic><topic>Reactive Oxygen Species</topic><topic>Serum albumin</topic><topic>Transport</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ambrogi, M</creatorcontrib><creatorcontrib>Dall'Acqua, PC</creatorcontrib><creatorcontrib>Rocha‐Frigoni, NAS</creatorcontrib><creatorcontrib>Leão, BCS</creatorcontrib><creatorcontrib>Mingoti, GZ</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ambrogi, M</au><au>Dall'Acqua, PC</au><au>Rocha‐Frigoni, NAS</au><au>Leão, BCS</au><au>Mingoti, GZ</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2017-06</date><risdate>2017</risdate><volume>52</volume><issue>3</issue><spage>409</spage><epage>421</epage><pages>409-421</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox). The intracellular concentrations of reactive oxygen species (ROS) at the end of IVM period were lower in the oocytes subjected to simulated transport in the presence of a macromolecular supplement or the antioxidant mixture than that of the control group (FCS: 0.62 and BSA: 0.66 vs. Control FCS: 1.00, p < .05; and Transp: 0.58 and Transp Antiox: 0.70 vs. Control FCS: 1.00, p < .05). After IVM, the mitochondrial membrane potentials of the transported oocytes were lower than those of the non‐transported oocytes (FCS: 0.41 and BSA: 0.57 vs. Control FCS: 1.00, p < .05; and Transp: 0.48 and Transp Antiox: 0.51 vs. Control FCS: 1.00 and Control Antiox: 0.84, p < .05). The blastocyst formation rates (36.9% average) and the re‐expansion rates of vitrified‐warmed blastocysts (53%, average) were unaffected (p > .05) by the treatments. In conclusion, supplementing the medium in which bovine oocytes are transported with antioxidants or different macromolecules did not affect their in vitro production of embryos or their cryotolerance.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>28120355</pmid><doi>10.1111/rda.12923</doi><tpages>13</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2017-06, Vol.52 (3), p.409-421 |
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| subjects | Animals Antioxidants Antioxidants - pharmacology Blastocysts Bovine serum albumin Catalase Catalase - pharmacology Cattle Cold tolerance Culture Media Cysteamine Cysteamine - pharmacology Cysteine Cysteine - pharmacology Cytoplasm - physiology Embryo Culture Techniques - veterinary Embryogenesis Embryonic growth stage Embryos In Vitro Oocyte Maturation Techniques - methods In Vitro Oocyte Maturation Techniques - veterinary In vitro testing Macromolecules Maturation Membrane Potential, Mitochondrial Mitochondria Oocytes Reactive Oxygen Species Serum albumin Transport |
| title | Transporting bovine oocytes in a medium supplemented with different macromolecules and antioxidants: Effects on nuclear and cytoplasmic maturation and embryonic development in vitro |
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