Agrobacterium-mediated transformation of a doubled haploid line of cabbage
Doubled haploid lines, which have high levels of genetic uniformity, are suitable for physiological or genetic studies. We established a transformation system using a doubled haploid line of cabbage. Hypocotyl explants that had been precultured for 3 days on Murashige and Skoog medium containing 50...
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| Veröffentlicht in: | Plant cell reports 2002-10, Vol.21 (3), p.257-262 |
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| creator | TSUKAZAKI, H KUGINUKI, Y AIDA, R SUZUKI, T |
| description | Doubled haploid lines, which have high levels of genetic uniformity, are suitable for physiological or genetic studies. We established a transformation system using a doubled haploid line of cabbage. Hypocotyl explants that had been precultured for 3 days on Murashige and Skoog medium containing 50 µM acetosyringone were inoculated and cocultured with Agrobacterium tumefaciens strain LBA4404 (pIG121Hm) for 3 days. Kanamycin-tolerant shoots were regenerated onto shoot induction medium 3 months after Agrobacterium inoculation. The transformation efficiency was about 3% under optimal conditions. The segregation pattern of the self-pollinated transformant that carried one copy of the introduced transgene revealed that the β-glucuronidase gene was inherited in a 3:1 ratio.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s00299-002-0506-4 |
| format | Article |
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| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Crop science Fundamental and applied biological sciences. Psychology Genetic engineering Genetic engineering applications Genetic technics Genetics and breeding of economic plants Genomics Methods. Procedures. Technologies Plant breeding: fundamental aspects and methodology Transgenic animals and transgenic plants Transgenic plants |
| title | Agrobacterium-mediated transformation of a doubled haploid line of cabbage |
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