Catechol Removal from Aqueous Media Using Laccase Immobilized in Different Macro- and Microreactor Systems
Laccase belongs to the group of enzymes that are capable to catalyze the oxidation of phenols. Since the water is only by-product in laccase-catalyzed phenol oxidations, it is ideally “green” enzyme with many possible applications in different industrial processes. To make the oxidation process more...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2017-08, Vol.182 (4), p.1575-1590 |
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| creator | Tusek, Ana Jurinjak Salic, Anita Zelic, Bruno |
| description | Laccase belongs to the group of enzymes that are capable to catalyze the oxidation of phenols. Since the water is only by-product in laccase-catalyzed phenol oxidations, it is ideally “green” enzyme with many possible applications in different industrial processes. To make the oxidation process more sustainable in terms of biocatalyst consumption, immobilization of the enzyme is implemented in to the processes. Additionally, when developing a process, choice of a reactor type plays a significant role in the total outcome.
In this study, the use of immobilized laccase from
Trametes versicolor
for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation, biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favor of microreactors with enzyme covalently immobilized on the microchannel surface. |
| doi_str_mv | 10.1007/s12010-017-2419-2 |
| format | Article |
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In this study, the use of immobilized laccase from
Trametes versicolor
for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation, biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favor of microreactors with enzyme covalently immobilized on the microchannel surface.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-017-2419-2</identifier><identifier>PMID: 28116574</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aqueous chemistry ; Bacteria ; Biocatalysis ; Biocatalysts ; Biochemistry ; Bioreactors - microbiology ; Biotechnology ; Catechol ; Catechols - isolation & purification ; Catechols - metabolism ; Chemistry ; Chemistry and Materials Science ; Covalence ; Enzymes ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Immobilization ; Laccase ; Laccase - chemistry ; Laccase - metabolism ; Microreactors ; Oxidation ; Oxidation process ; Oxidation-Reduction ; Phenols ; Reactors ; Trametes - enzymology ; Water - chemistry ; Water Pollutants, Chemical - isolation & purification ; Water Pollutants, Chemical - metabolism</subject><ispartof>Applied biochemistry and biotechnology, 2017-08, Vol.182 (4), p.1575-1590</ispartof><rights>Springer Science+Business Media New York 2017</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-bc02b1d02d75c68816747786aa955fb2dc0e77d71f7007eb23a34f240fa0629a3</citedby><cites>FETCH-LOGICAL-c409t-bc02b1d02d75c68816747786aa955fb2dc0e77d71f7007eb23a34f240fa0629a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-017-2419-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-017-2419-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28116574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tusek, Ana Jurinjak</creatorcontrib><creatorcontrib>Salic, Anita</creatorcontrib><creatorcontrib>Zelic, Bruno</creatorcontrib><title>Catechol Removal from Aqueous Media Using Laccase Immobilized in Different Macro- and Microreactor Systems</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Laccase belongs to the group of enzymes that are capable to catalyze the oxidation of phenols. Since the water is only by-product in laccase-catalyzed phenol oxidations, it is ideally “green” enzyme with many possible applications in different industrial processes. To make the oxidation process more sustainable in terms of biocatalyst consumption, immobilization of the enzyme is implemented in to the processes. Additionally, when developing a process, choice of a reactor type plays a significant role in the total outcome.
In this study, the use of immobilized laccase from
Trametes versicolor
for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation, biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favor of microreactors with enzyme covalently immobilized on the microchannel surface.</description><subject>Aqueous chemistry</subject><subject>Bacteria</subject><subject>Biocatalysis</subject><subject>Biocatalysts</subject><subject>Biochemistry</subject><subject>Bioreactors - microbiology</subject><subject>Biotechnology</subject><subject>Catechol</subject><subject>Catechols - isolation & purification</subject><subject>Catechols - metabolism</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Covalence</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Immobilization</subject><subject>Laccase</subject><subject>Laccase - chemistry</subject><subject>Laccase - metabolism</subject><subject>Microreactors</subject><subject>Oxidation</subject><subject>Oxidation process</subject><subject>Oxidation-Reduction</subject><subject>Phenols</subject><subject>Reactors</subject><subject>Trametes - enzymology</subject><subject>Water - chemistry</subject><subject>Water Pollutants, Chemical - isolation & purification</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU-LFDEQxYMo7uzoB_AiAS9eolXVf9I5LrO6LswgqHsO6XSyZujurEmPsH56M84qIgiBCtSvXvLqMfYC4Q0CyLcZCRAEoBRUoxL0iK2waZQAUviYrYBkJYg6dcbOc94DIHWNfMrOqENsG1mv2H5jFme_xpF_clP8bkbuU5z4xbeDi4fMd24Iht_kMN_yrbHWZMevpyn2YQw_3MDDzC-D9y65eeE7Y1MU3MwD34VyTc7YJSb--T4vbsrP2BNvxuyeP9Q1u3n_7svmg9h-vLreXGyFrUEtordAPQ5Ag2xs23XYylrKrjVGNY3vabDgpBwkell24HqqTFV7qsEbaEmZas1en3TvUiw28qKnkK0bRzMfPWnsWmyhVV1b0Ff_oPt4SHP5nUZFdYW_zprhiSqeck7O67sUJpPuNYI-BqFPQegShD4GoanMvHxQPvSTG_5M_N58AegE5NKab1366-n_qv4ELD2R_A</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Tusek, Ana Jurinjak</creator><creator>Salic, Anita</creator><creator>Zelic, Bruno</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20170801</creationdate><title>Catechol Removal from Aqueous Media Using Laccase Immobilized in Different Macro- and Microreactor Systems</title><author>Tusek, Ana Jurinjak ; Salic, Anita ; Zelic, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-bc02b1d02d75c68816747786aa955fb2dc0e77d71f7007eb23a34f240fa0629a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aqueous chemistry</topic><topic>Bacteria</topic><topic>Biocatalysis</topic><topic>Biocatalysts</topic><topic>Biochemistry</topic><topic>Bioreactors - microbiology</topic><topic>Biotechnology</topic><topic>Catechol</topic><topic>Catechols - isolation & purification</topic><topic>Catechols - metabolism</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Covalence</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Immobilization</topic><topic>Laccase</topic><topic>Laccase - chemistry</topic><topic>Laccase - metabolism</topic><topic>Microreactors</topic><topic>Oxidation</topic><topic>Oxidation process</topic><topic>Oxidation-Reduction</topic><topic>Phenols</topic><topic>Reactors</topic><topic>Trametes - enzymology</topic><topic>Water - chemistry</topic><topic>Water Pollutants, Chemical - isolation & purification</topic><topic>Water Pollutants, Chemical - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tusek, Ana Jurinjak</creatorcontrib><creatorcontrib>Salic, Anita</creatorcontrib><creatorcontrib>Zelic, Bruno</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tusek, Ana Jurinjak</au><au>Salic, Anita</au><au>Zelic, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Catechol Removal from Aqueous Media Using Laccase Immobilized in Different Macro- and Microreactor Systems</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>182</volume><issue>4</issue><spage>1575</spage><epage>1590</epage><pages>1575-1590</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Laccase belongs to the group of enzymes that are capable to catalyze the oxidation of phenols. Since the water is only by-product in laccase-catalyzed phenol oxidations, it is ideally “green” enzyme with many possible applications in different industrial processes. To make the oxidation process more sustainable in terms of biocatalyst consumption, immobilization of the enzyme is implemented in to the processes. Additionally, when developing a process, choice of a reactor type plays a significant role in the total outcome.
In this study, the use of immobilized laccase from
Trametes versicolor
for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation, biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favor of microreactors with enzyme covalently immobilized on the microchannel surface.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28116574</pmid><doi>10.1007/s12010-017-2419-2</doi><tpages>16</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2017-08, Vol.182 (4), p.1575-1590 |
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| subjects | Aqueous chemistry Bacteria Biocatalysis Biocatalysts Biochemistry Bioreactors - microbiology Biotechnology Catechol Catechols - isolation & purification Catechols - metabolism Chemistry Chemistry and Materials Science Covalence Enzymes Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Immobilization Laccase Laccase - chemistry Laccase - metabolism Microreactors Oxidation Oxidation process Oxidation-Reduction Phenols Reactors Trametes - enzymology Water - chemistry Water Pollutants, Chemical - isolation & purification Water Pollutants, Chemical - metabolism |
| title | Catechol Removal from Aqueous Media Using Laccase Immobilized in Different Macro- and Microreactor Systems |
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