Life and Death of Yeast Telomerase RNA

Telomerase reverse transcriptase elongates telomeres to overcome their natural attrition and allow unlimited cellular proliferation, a characteristic shared by stem cells and the majority of malignant cancerous cells. The telomerase holoenzyme comprises a core RNA molecule, a catalytic protein subun...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of molecular biology 2017-10, Vol.429 (21), p.3242-3254
Hauptverfasser: Vasianovich, Yulia, Wellinger, Raymund J.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 3254
container_issue 21
container_start_page 3242
container_title Journal of molecular biology
container_volume 429
creator Vasianovich, Yulia
Wellinger, Raymund J.
description Telomerase reverse transcriptase elongates telomeres to overcome their natural attrition and allow unlimited cellular proliferation, a characteristic shared by stem cells and the majority of malignant cancerous cells. The telomerase holoenzyme comprises a core RNA molecule, a catalytic protein subunit, and other accessory proteins. Malfunction of certain telomerase components can cause serious genetic disorders including dyskeratosis congenita and aplastic anaemia. A hierarchy of tightly regulated steps constitutes the process of telomerase biogenesis, which, if interrupted or misregulated, can impede the production of a functional enzyme and severely affect telomere maintenance. Here, we take a closer look at the budding yeast telomerase RNA component, TLC1, in its long lifetime journey around the cell. We review the extensive knowledge on TLC1 transcription and processing. We focus on exciting recent studies on telomerase assembly, trafficking, and nuclear dynamics, which for the first time unveil striking similarities between the yeast and human telomerase ribonucleoproteins. Finally, we identify questions yet to be answered and new directions to be followed, which, in the future, might improve our knowledge of telomerase biology and trigger the development of new therapies against cancer and other telomerase-related diseases. [Display omitted] •Yeast telomerase is composed of a single RNA bound by Est1-3 and Pop1, 6, and 7 proteins.•The ribonucleoprotein diffuses freely in the nucleus, forming rare stable interactions with telomeres.•Yeast telomeric repeat-containing RNA clusters telomerase molecules and directs them to short telomeres.•Cajal bodies are not essential for telomerase function or its delivery to telomeres.
doi_str_mv 10.1016/j.jmb.2017.01.013
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1861600684</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022283617300414</els_id><sourcerecordid>1861600684</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-36f034a46ceb841bec114ecd2a666e4b0c34b6ea8865f69b516ba0da94ddfe9a3</originalsourceid><addsrcrecordid>eNp9kE1Lw0AURQdRbK3-ADeSlbhJfS8zGSe4kvoJRUHqwtUwmXnBhKSpM6ngv3dKq0vhwtuce-Edxk4RpggoL5tp05XTDPBqChjD99gYQRWpklztszFAlqWZ4nLEjkJoACDnQh2yUaYQ89gbs_N5XVFili65JTN8JH2VvJMJQ7Kgtu_Im0DJ6_PNMTuoTBvoZHcn7O3-bjF7TOcvD0-zm3lqec6HlMsKuDBCWiqVwJIsoiDrMiOlJFGC5aKUZJSSeSWLMkdZGnCmEM5VVBg-YRfb3ZXvP9cUBt3VwVLbmiX166BRSZQAUomI4ha1vg_BU6VXvu6M_9YIeqNHNzrq0Rs9GjCGx87Zbn5dduT-Gr8-InC9BSg--VWT18HWtLTkak920K6v_5n_AaTJcxg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1861600684</pqid></control><display><type>article</type><title>Life and Death of Yeast Telomerase RNA</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Vasianovich, Yulia ; Wellinger, Raymund J.</creator><creatorcontrib>Vasianovich, Yulia ; Wellinger, Raymund J.</creatorcontrib><description>Telomerase reverse transcriptase elongates telomeres to overcome their natural attrition and allow unlimited cellular proliferation, a characteristic shared by stem cells and the majority of malignant cancerous cells. The telomerase holoenzyme comprises a core RNA molecule, a catalytic protein subunit, and other accessory proteins. Malfunction of certain telomerase components can cause serious genetic disorders including dyskeratosis congenita and aplastic anaemia. A hierarchy of tightly regulated steps constitutes the process of telomerase biogenesis, which, if interrupted or misregulated, can impede the production of a functional enzyme and severely affect telomere maintenance. Here, we take a closer look at the budding yeast telomerase RNA component, TLC1, in its long lifetime journey around the cell. We review the extensive knowledge on TLC1 transcription and processing. We focus on exciting recent studies on telomerase assembly, trafficking, and nuclear dynamics, which for the first time unveil striking similarities between the yeast and human telomerase ribonucleoproteins. Finally, we identify questions yet to be answered and new directions to be followed, which, in the future, might improve our knowledge of telomerase biology and trigger the development of new therapies against cancer and other telomerase-related diseases. [Display omitted] •Yeast telomerase is composed of a single RNA bound by Est1-3 and Pop1, 6, and 7 proteins.•The ribonucleoprotein diffuses freely in the nucleus, forming rare stable interactions with telomeres.•Yeast telomeric repeat-containing RNA clusters telomerase molecules and directs them to short telomeres.•Cajal bodies are not essential for telomerase function or its delivery to telomeres.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2017.01.013</identifier><identifier>PMID: 28115201</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Humans ; RNA - genetics ; RNA, Fungal - genetics ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Telomerase - genetics ; telomerase biologenesis ; telomerase RNA ; Telomere - metabolism</subject><ispartof>Journal of molecular biology, 2017-10, Vol.429 (21), p.3242-3254</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-36f034a46ceb841bec114ecd2a666e4b0c34b6ea8865f69b516ba0da94ddfe9a3</citedby><cites>FETCH-LOGICAL-c353t-36f034a46ceb841bec114ecd2a666e4b0c34b6ea8865f69b516ba0da94ddfe9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmb.2017.01.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28115201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vasianovich, Yulia</creatorcontrib><creatorcontrib>Wellinger, Raymund J.</creatorcontrib><title>Life and Death of Yeast Telomerase RNA</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Telomerase reverse transcriptase elongates telomeres to overcome their natural attrition and allow unlimited cellular proliferation, a characteristic shared by stem cells and the majority of malignant cancerous cells. The telomerase holoenzyme comprises a core RNA molecule, a catalytic protein subunit, and other accessory proteins. Malfunction of certain telomerase components can cause serious genetic disorders including dyskeratosis congenita and aplastic anaemia. A hierarchy of tightly regulated steps constitutes the process of telomerase biogenesis, which, if interrupted or misregulated, can impede the production of a functional enzyme and severely affect telomere maintenance. Here, we take a closer look at the budding yeast telomerase RNA component, TLC1, in its long lifetime journey around the cell. We review the extensive knowledge on TLC1 transcription and processing. We focus on exciting recent studies on telomerase assembly, trafficking, and nuclear dynamics, which for the first time unveil striking similarities between the yeast and human telomerase ribonucleoproteins. Finally, we identify questions yet to be answered and new directions to be followed, which, in the future, might improve our knowledge of telomerase biology and trigger the development of new therapies against cancer and other telomerase-related diseases. [Display omitted] •Yeast telomerase is composed of a single RNA bound by Est1-3 and Pop1, 6, and 7 proteins.•The ribonucleoprotein diffuses freely in the nucleus, forming rare stable interactions with telomeres.•Yeast telomeric repeat-containing RNA clusters telomerase molecules and directs them to short telomeres.•Cajal bodies are not essential for telomerase function or its delivery to telomeres.</description><subject>Humans</subject><subject>RNA - genetics</subject><subject>RNA, Fungal - genetics</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Telomerase - genetics</subject><subject>telomerase biologenesis</subject><subject>telomerase RNA</subject><subject>Telomere - metabolism</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AURQdRbK3-ADeSlbhJfS8zGSe4kvoJRUHqwtUwmXnBhKSpM6ngv3dKq0vhwtuce-Edxk4RpggoL5tp05XTDPBqChjD99gYQRWpklztszFAlqWZ4nLEjkJoACDnQh2yUaYQ89gbs_N5XVFili65JTN8JH2VvJMJQ7Kgtu_Im0DJ6_PNMTuoTBvoZHcn7O3-bjF7TOcvD0-zm3lqec6HlMsKuDBCWiqVwJIsoiDrMiOlJFGC5aKUZJSSeSWLMkdZGnCmEM5VVBg-YRfb3ZXvP9cUBt3VwVLbmiX166BRSZQAUomI4ha1vg_BU6VXvu6M_9YIeqNHNzrq0Rs9GjCGx87Zbn5dduT-Gr8-InC9BSg--VWT18HWtLTkak920K6v_5n_AaTJcxg</recordid><startdate>20171027</startdate><enddate>20171027</enddate><creator>Vasianovich, Yulia</creator><creator>Wellinger, Raymund J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20171027</creationdate><title>Life and Death of Yeast Telomerase RNA</title><author>Vasianovich, Yulia ; Wellinger, Raymund J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-36f034a46ceb841bec114ecd2a666e4b0c34b6ea8865f69b516ba0da94ddfe9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Humans</topic><topic>RNA - genetics</topic><topic>RNA, Fungal - genetics</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Telomerase - genetics</topic><topic>telomerase biologenesis</topic><topic>telomerase RNA</topic><topic>Telomere - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vasianovich, Yulia</creatorcontrib><creatorcontrib>Wellinger, Raymund J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasianovich, Yulia</au><au>Wellinger, Raymund J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Life and Death of Yeast Telomerase RNA</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2017-10-27</date><risdate>2017</risdate><volume>429</volume><issue>21</issue><spage>3242</spage><epage>3254</epage><pages>3242-3254</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Telomerase reverse transcriptase elongates telomeres to overcome their natural attrition and allow unlimited cellular proliferation, a characteristic shared by stem cells and the majority of malignant cancerous cells. The telomerase holoenzyme comprises a core RNA molecule, a catalytic protein subunit, and other accessory proteins. Malfunction of certain telomerase components can cause serious genetic disorders including dyskeratosis congenita and aplastic anaemia. A hierarchy of tightly regulated steps constitutes the process of telomerase biogenesis, which, if interrupted or misregulated, can impede the production of a functional enzyme and severely affect telomere maintenance. Here, we take a closer look at the budding yeast telomerase RNA component, TLC1, in its long lifetime journey around the cell. We review the extensive knowledge on TLC1 transcription and processing. We focus on exciting recent studies on telomerase assembly, trafficking, and nuclear dynamics, which for the first time unveil striking similarities between the yeast and human telomerase ribonucleoproteins. Finally, we identify questions yet to be answered and new directions to be followed, which, in the future, might improve our knowledge of telomerase biology and trigger the development of new therapies against cancer and other telomerase-related diseases. [Display omitted] •Yeast telomerase is composed of a single RNA bound by Est1-3 and Pop1, 6, and 7 proteins.•The ribonucleoprotein diffuses freely in the nucleus, forming rare stable interactions with telomeres.•Yeast telomeric repeat-containing RNA clusters telomerase molecules and directs them to short telomeres.•Cajal bodies are not essential for telomerase function or its delivery to telomeres.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>28115201</pmid><doi>10.1016/j.jmb.2017.01.013</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-2836
ispartof Journal of molecular biology, 2017-10, Vol.429 (21), p.3242-3254
issn 0022-2836
1089-8638
language eng
recordid cdi_proquest_miscellaneous_1861600684
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Humans
RNA - genetics
RNA, Fungal - genetics
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Telomerase - genetics
telomerase biologenesis
telomerase RNA
Telomere - metabolism
title Life and Death of Yeast Telomerase RNA
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T01%3A13%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Life%20and%20Death%20of%20Yeast%20Telomerase%20RNA&rft.jtitle=Journal%20of%20molecular%20biology&rft.au=Vasianovich,%20Yulia&rft.date=2017-10-27&rft.volume=429&rft.issue=21&rft.spage=3242&rft.epage=3254&rft.pages=3242-3254&rft.issn=0022-2836&rft.eissn=1089-8638&rft_id=info:doi/10.1016/j.jmb.2017.01.013&rft_dat=%3Cproquest_cross%3E1861600684%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1861600684&rft_id=info:pmid/28115201&rft_els_id=S0022283617300414&rfr_iscdi=true