Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase

The luciferase reporter assay has become one of the conventional methods for cytotoxicity evaluation. Typically, the decrease of luminescence expressed by a constitutive promoter is used as an index of cytotoxicity. However, to our knowledge, there have been no reports of the correlation between cyt...

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Veröffentlicht in:	Analytical biochemistry 2017-04, Vol.522, p.18-29
Hauptverfasser:	Wakuri, S., Yamakage, K., Kazuki, Y., Kazuki, K., Oshimura, M., Aburatani, S., Yasunaga, M., Nakajima, Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromosomes, Artificial, Mammalian - genetics Chromosomes, Artificial, Mammalian - metabolism Coleoptera Cytotoxicity Hep G2 Cells Humans Insect Proteins - genetics Insect Proteins - metabolism Internal control reporter Luciferase Luciferase reporter assay Luciferases - genetics Luciferases - metabolism Luminescent Measurements - methods Mice Promoter Regions, Genetic Toxicity Tests - methods
Online-Zugang:	Volltext
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