A Quantitative Evaluation of a 2.5 kb Rat Tyrosine Hydroxylase Promoter to Target Expression in Ventral Mesencephalic Dopamine Neurons In Vivo
Highlights • AAV vectors with specific promoters for neuron phenotypes are powerful tools in neuroscience, particularly in optogenetics. • Midbrain dopamine neurons have great interest because they play a central role in neurodegenerative and psychiatric disease. • To facilitate their study we analy...
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| Veröffentlicht in: | Neuroscience 2017-03, Vol.346, p.126-134 |
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| creator | Rolland, Anne-Sophie Kareva, Tatyana Kholodilov, Nikolai Burke, Robert E |
| description | Highlights • AAV vectors with specific promoters for neuron phenotypes are powerful tools in neuroscience, particularly in optogenetics. • Midbrain dopamine neurons have great interest because they play a central role in neurodegenerative and psychiatric disease. • To facilitate their study we analyzed specificity and efficiency of a 2.5 kb sequence of rat tyrosine hydroxylase promoter. • Regional specificity was good, as no expression was seen in striatum or cortex, but cellular specificity in SN was only 50% • This promoter has utility in the study of dopaminergic neurons, but incomplete specificity necessitates caution in its use. |
| doi_str_mv | 10.1016/j.neuroscience.2017.01.014 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Neuroscience, 2017-03, Vol.346, p.126-134 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | AAV Animals Dependovirus - physiology dopamine Dopaminergic Neurons - metabolism Genetic Vectors Immunohistochemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Male Mice, Inbred C57BL Neurology Optogenetics promoter Promoter Regions, Genetic Rats Red Fluorescent Protein substantia nigra Substantia Nigra - metabolism Transgenes Tyrosine 3-Monooxygenase - genetics Tyrosine 3-Monooxygenase - metabolism tyrosine hydroxylase |
| title | A Quantitative Evaluation of a 2.5 kb Rat Tyrosine Hydroxylase Promoter to Target Expression in Ventral Mesencephalic Dopamine Neurons In Vivo |
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