Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response‐like cell death
The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant‐specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remain...
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| Veröffentlicht in: | Physiologia plantarum 2017-06, Vol.160 (2), p.209-221 |
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| creator | Chen, Qinqin Niu, Fangfang Yan, Jingli Chen, Bisi Wu, Feifei Guo, Xiaohua Yang, Bo Jiang, Yuan‐Qing |
| description | The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant‐specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. In the present study, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold. BnaNAC56 is a transcription activator and localized to nuclei. Overexpression of BnaNAC56 induced reactive oxygen species (ROS) accumulation and hypersensitive response (HR)‐like cell death, with various physiological measurements supporting these. Furthermore, BnaNAC56 expression caused evident nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT‐PCR) analysis identified that the expression levels of multiple genes regulating ROS homeostasis, cell death and defense response were significantly induced. Using a dual luciferase reporter assay, we further confirmed that BnaNAC56 could activate the expression of a few ROS‐ and cell death‐related genes. In summary, our data demonstrate that BnaNAC56 functions as a stress‐responsive transcriptional activator and plays a role in modulating ROS accumulation and cell death. |
| doi_str_mv | 10.1111/ppl.12545 |
| format | Article |
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Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. In the present study, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold. BnaNAC56 is a transcription activator and localized to nuclei. Overexpression of BnaNAC56 induced reactive oxygen species (ROS) accumulation and hypersensitive response (HR)‐like cell death, with various physiological measurements supporting these. Furthermore, BnaNAC56 expression caused evident nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT‐PCR) analysis identified that the expression levels of multiple genes regulating ROS homeostasis, cell death and defense response were significantly induced. Using a dual luciferase reporter assay, we further confirmed that BnaNAC56 could activate the expression of a few ROS‐ and cell death‐related genes. In summary, our data demonstrate that BnaNAC56 functions as a stress‐responsive transcriptional activator and plays a role in modulating ROS accumulation and cell death.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.12545</identifier><identifier>PMID: 28097691</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Abscisic acid ; Abscisic Acid - metabolism ; Accumulation ; Apoptosis ; Ascomycota - physiology ; Assaying ; Brassica ; Brassica napus - genetics ; Brassica napus - metabolism ; Brassica napus - microbiology ; Cell death ; Cell Death - genetics ; Cyclopentanes - metabolism ; Deoxyribonucleic acid ; DNA ; DNA fragmentation ; Expressed Sequence Tags ; Fragmentation ; Fungi ; Gene expression ; Gene Expression Regulation, Plant - genetics ; Genes ; Homeostasis ; Hypersensitive response ; Jasmonic acid ; Methyl viologen ; Mortality ; Nuclei ; Oilseeds ; Oxygen ; Oxylipins - metabolism ; Paraquat - metabolism ; Pathogens ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polymerase chain reaction ; Rape plants ; Rapeseed ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Reverse transcription ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Physiologia plantarum, 2017-06, Vol.160 (2), p.209-221</ispartof><rights>2017 Scandinavian Plant Physiology Society</rights><rights>2017 Scandinavian Plant Physiology Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-d2902f48d9118a01566dd725dff5a339cb9e677d2d8141b2eae69095a5f5c83a3</citedby><cites>FETCH-LOGICAL-c3535-d2902f48d9118a01566dd725dff5a339cb9e677d2d8141b2eae69095a5f5c83a3</cites><orcidid>0000-0003-1367-922X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppl.12545$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppl.12545$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28097691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Qinqin</creatorcontrib><creatorcontrib>Niu, Fangfang</creatorcontrib><creatorcontrib>Yan, Jingli</creatorcontrib><creatorcontrib>Chen, Bisi</creatorcontrib><creatorcontrib>Wu, Feifei</creatorcontrib><creatorcontrib>Guo, Xiaohua</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Jiang, Yuan‐Qing</creatorcontrib><title>Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response‐like cell death</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant‐specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. In the present study, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold. BnaNAC56 is a transcription activator and localized to nuclei. Overexpression of BnaNAC56 induced reactive oxygen species (ROS) accumulation and hypersensitive response (HR)‐like cell death, with various physiological measurements supporting these. Furthermore, BnaNAC56 expression caused evident nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT‐PCR) analysis identified that the expression levels of multiple genes regulating ROS homeostasis, cell death and defense response were significantly induced. Using a dual luciferase reporter assay, we further confirmed that BnaNAC56 could activate the expression of a few ROS‐ and cell death‐related genes. In summary, our data demonstrate that BnaNAC56 functions as a stress‐responsive transcriptional activator and plays a role in modulating ROS accumulation and cell death.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Accumulation</subject><subject>Apoptosis</subject><subject>Ascomycota - physiology</subject><subject>Assaying</subject><subject>Brassica</subject><subject>Brassica napus - genetics</subject><subject>Brassica napus - metabolism</subject><subject>Brassica napus - microbiology</subject><subject>Cell death</subject><subject>Cell Death - genetics</subject><subject>Cyclopentanes - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fragmentation</subject><subject>Expressed Sequence Tags</subject><subject>Fragmentation</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Genes</subject><subject>Homeostasis</subject><subject>Hypersensitive response</subject><subject>Jasmonic acid</subject><subject>Methyl viologen</subject><subject>Mortality</subject><subject>Nuclei</subject><subject>Oilseeds</subject><subject>Oxygen</subject><subject>Oxylipins - metabolism</subject><subject>Paraquat - metabolism</subject><subject>Pathogens</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Reverse transcription</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10cFO3DAQBmCrKioL7aEvUFnqpRwCnnjtxEe0KqXSqnCAc-S1J2CaxK6d0O6NQx-gz9gnwcsCByR8sWR9_jWan5CPwA4hn6MQukMoxVy8ITPgShWciflbMmOMQ6E4VLtkL6UbxkBKKN-R3bJmqpIKZuTvmesSoqVRB6Q_jhdC0jHqIZnowuj8QFttRh9p7-3U6RETjZhf3C1S_2d9hQNNAY3L79qYqd-YzS89WHq9DhgTDsk98Igp-CHh_7t_nfuJ1GDXUYt6vH5Pdlqdx_jweO-Ty5OvF4vTYnn27fvieFkYLrgobKlY2c5rqwBqzUBIaW1VCtu2QnOuzEqhrCpb2hrmsCpRo1RMCS1aYWqu-T75ss0N0f-aMI1N79JmDD2gn1IDtQRRsbyYTD-_oDd-ikOeLitV1QzyLrM62CoTfUoR2yZE1-u4boA1m2qaXE3zUE22nx4Tp1WP9lk-dZHB0Rb8dh2uX09qzs-X28h7KKWa6A</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Chen, Qinqin</creator><creator>Niu, Fangfang</creator><creator>Yan, Jingli</creator><creator>Chen, Bisi</creator><creator>Wu, Feifei</creator><creator>Guo, Xiaohua</creator><creator>Yang, Bo</creator><creator>Jiang, Yuan‐Qing</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1367-922X</orcidid></search><sort><creationdate>201706</creationdate><title>Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response‐like cell death</title><author>Chen, Qinqin ; Niu, Fangfang ; Yan, Jingli ; Chen, Bisi ; Wu, Feifei ; Guo, Xiaohua ; Yang, Bo ; Jiang, Yuan‐Qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-d2902f48d9118a01566dd725dff5a339cb9e677d2d8141b2eae69095a5f5c83a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Accumulation</topic><topic>Apoptosis</topic><topic>Ascomycota - physiology</topic><topic>Assaying</topic><topic>Brassica</topic><topic>Brassica napus - genetics</topic><topic>Brassica napus - metabolism</topic><topic>Brassica napus - microbiology</topic><topic>Cell death</topic><topic>Cell Death - genetics</topic><topic>Cyclopentanes - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fragmentation</topic><topic>Expressed Sequence Tags</topic><topic>Fragmentation</topic><topic>Fungi</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Genes</topic><topic>Homeostasis</topic><topic>Hypersensitive response</topic><topic>Jasmonic acid</topic><topic>Methyl viologen</topic><topic>Mortality</topic><topic>Nuclei</topic><topic>Oilseeds</topic><topic>Oxygen</topic><topic>Oxylipins - metabolism</topic><topic>Paraquat - metabolism</topic><topic>Pathogens</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Rape plants</topic><topic>Rapeseed</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Reverse transcription</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Qinqin</creatorcontrib><creatorcontrib>Niu, Fangfang</creatorcontrib><creatorcontrib>Yan, Jingli</creatorcontrib><creatorcontrib>Chen, Bisi</creatorcontrib><creatorcontrib>Wu, Feifei</creatorcontrib><creatorcontrib>Guo, Xiaohua</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Jiang, Yuan‐Qing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Qinqin</au><au>Niu, Fangfang</au><au>Yan, Jingli</au><au>Chen, Bisi</au><au>Wu, Feifei</au><au>Guo, Xiaohua</au><au>Yang, Bo</au><au>Jiang, Yuan‐Qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response‐like cell death</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2017-06</date><risdate>2017</risdate><volume>160</volume><issue>2</issue><spage>209</spage><epage>221</epage><pages>209-221</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><abstract>The NAC (NAM, ATAF1/2, CUC2) transcription factor gene family is plant‐specific and plays diverse roles in development and responses to abiotic stresses and pathogen challenge. Oilseed rape (Brassica napus) or canola is an important oil crop worldwide, however, the function of NAC genes in it remains largely elusive. In the present study, we identified and characterized the NAC56 gene isolated from oilseed rape. Expression of BnaNAC56 was induced by abscisic acid (ABA), jasmonic acid (JA), methyl viologen (MV) and a necrotrophic fungal pathogen Sclerotinia sclerotiorum, but repressed by cold. BnaNAC56 is a transcription activator and localized to nuclei. Overexpression of BnaNAC56 induced reactive oxygen species (ROS) accumulation and hypersensitive response (HR)‐like cell death, with various physiological measurements supporting these. Furthermore, BnaNAC56 expression caused evident nuclear DNA fragmentation. Moreover, quantitative reverse transcription PCR (qRT‐PCR) analysis identified that the expression levels of multiple genes regulating ROS homeostasis, cell death and defense response were significantly induced. Using a dual luciferase reporter assay, we further confirmed that BnaNAC56 could activate the expression of a few ROS‐ and cell death‐related genes. In summary, our data demonstrate that BnaNAC56 functions as a stress‐responsive transcriptional activator and plays a role in modulating ROS accumulation and cell death.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>28097691</pmid><doi>10.1111/ppl.12545</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-1367-922X</orcidid></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - metabolism Accumulation Apoptosis Ascomycota - physiology Assaying Brassica Brassica napus - genetics Brassica napus - metabolism Brassica napus - microbiology Cell death Cell Death - genetics Cyclopentanes - metabolism Deoxyribonucleic acid DNA DNA fragmentation Expressed Sequence Tags Fragmentation Fungi Gene expression Gene Expression Regulation, Plant - genetics Genes Homeostasis Hypersensitive response Jasmonic acid Methyl viologen Mortality Nuclei Oilseeds Oxygen Oxylipins - metabolism Paraquat - metabolism Pathogens Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polymerase chain reaction Rape plants Rapeseed Reactive oxygen species Reactive Oxygen Species - metabolism Reverse transcription Transcription factors Transcription Factors - genetics Transcription Factors - metabolism |
| title | Oilseed rape NAC56 transcription factor modulates reactive oxygen species accumulation and hypersensitive response‐like cell death |
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