Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A
The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in Slovakia. Among all ages of animals, PKV-1 was dete...
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| description | The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in Slovakia. Among all ages of animals, PKV-1 was detected equally in diarrheic (63.8%) and clinically healthy (62.9%) pigs. PKV-1 was more often detected in diarrheic (74.6%) than in healthy (64.4%) suckling piglets (70days) of both healthy and diarrheic pigs were inconsistent ranging in interval 56.2% to 67.9%. This study did not confirm a clear relationship of PKV-1 infection with diarrhea in pigs. Rotavirus A infection was detected among the same animals in 39% diarrheic and 9.2% healthy suckling piglets (p |
| doi_str_mv | 10.1016/j.meegid.2017.01.011 |
| format | Article |
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•The prevalence of PKV-1 and rotavirus A was studied in pigs of different age.•PKV-1 was equally detected in diarrheic (63.8%) and healthy (62.9%) pigs.•No clear relationship between detection of PKV-1 in pigs and diarrhea was confirmed.•Rotavirus A infection was associated significantly (p<0.001) with diarrhea in piglets.•Slovakian PKV-1 sequences were clustered in the phylogenetic tree with Asian sequences.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2017.01.011</identifier><identifier>PMID: 28087494</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Animals, Suckling ; Asymptomatic Diseases ; Cluster Analysis ; Coinfection ; Diarrhea ; Diarrhea - epidemiology ; Diarrhea - virology ; Feces - virology ; Kobuvirus - classification ; Kobuvirus - genetics ; Kobuvirus - isolation & purification ; Phylogenetic analysis ; Phylogeny ; Picornaviridae Infections - epidemiology ; Picornaviridae Infections - transmission ; Picornaviridae Infections - veterinary ; Picornaviridae Infections - virology ; Porcine kobuvirus 1 ; RNA, Viral - genetics ; Rotavirus ; Rotavirus - classification ; Rotavirus - genetics ; Rotavirus - isolation & purification ; Rotavirus Infections - epidemiology ; Rotavirus Infections - transmission ; Rotavirus Infections - veterinary ; Rotavirus Infections - virology ; Sequence Analysis, DNA ; Severity of Illness Index ; Slovakia - epidemiology ; Swine ; Swine Diseases - epidemiology ; Swine Diseases - transmission ; Swine Diseases - virology ; Weaning</subject><ispartof>Infection, genetics and evolution, 2017-04, Vol.49, p.73-77</ispartof><rights>2017 The Authors</rights><rights>Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-6d24c7b29ad5aacf4c6d7681c75b5afd371443f69e3a9060045c1556e2a907a03</citedby><cites>FETCH-LOGICAL-c408t-6d24c7b29ad5aacf4c6d7681c75b5afd371443f69e3a9060045c1556e2a907a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.meegid.2017.01.011$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28087494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackova, Anna</creatorcontrib><creatorcontrib>Sliz, Ivan</creatorcontrib><creatorcontrib>Mandelik, Rene</creatorcontrib><creatorcontrib>Salamunova, Slavomira</creatorcontrib><creatorcontrib>Novotny, Jaroslav</creatorcontrib><creatorcontrib>Kolesarova, Mariana</creatorcontrib><creatorcontrib>Vlasakova, Michaela</creatorcontrib><creatorcontrib>Vilcek, Stefan</creatorcontrib><title>Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in Slovakia. Among all ages of animals, PKV-1 was detected equally in diarrheic (63.8%) and clinically healthy (62.9%) pigs. PKV-1 was more often detected in diarrheic (74.6%) than in healthy (64.4%) suckling piglets (<28days) but data was not statistically significant. Results in weaned (28–70days) and fattening (>70days) of both healthy and diarrheic pigs were inconsistent ranging in interval 56.2% to 67.9%. This study did not confirm a clear relationship of PKV-1 infection with diarrhea in pigs. Rotavirus A infection was detected among the same animals in 39% diarrheic and 9.2% healthy suckling piglets (p<0.001) confirming rotavirus as a causative agent of diarrhea in this age group. The difference was not significant in older pigs with both diarrheic and healthy pigs being infected within a range of 0% to 12.2%. Co-infection with PKV-1 and rotavirus A was detected overall in 5.6% of healthy and in 13.5% of diarrheic pigs and was highest in suckling piglets (33.9%). The PKV-1sequences from pigs in Slovakia were analyzed at the genetic level in the partial 3D gene region for the first time. The viral sequences were grouped in phylogenetic clusters according to their farm of origin. When compared with 157 nucleotide sequences originating from pig samples of different countries around the world Slovakian PKV-1 sequences were clustered in the phylogenetic tree with Asian sequences but not with nucleotide sequences from the neighbouring countries of Czech Republic or Hungary.
•The prevalence of PKV-1 and rotavirus A was studied in pigs of different age.•PKV-1 was equally detected in diarrheic (63.8%) and healthy (62.9%) pigs.•No clear relationship between detection of PKV-1 in pigs and diarrhea was confirmed.•Rotavirus A infection was associated significantly (p<0.001) with diarrhea in piglets.•Slovakian PKV-1 sequences were clustered in the phylogenetic tree with Asian sequences.</description><subject>Animals</subject><subject>Animals, Suckling</subject><subject>Asymptomatic Diseases</subject><subject>Cluster Analysis</subject><subject>Coinfection</subject><subject>Diarrhea</subject><subject>Diarrhea - epidemiology</subject><subject>Diarrhea - virology</subject><subject>Feces - virology</subject><subject>Kobuvirus - classification</subject><subject>Kobuvirus - genetics</subject><subject>Kobuvirus - isolation & purification</subject><subject>Phylogenetic analysis</subject><subject>Phylogeny</subject><subject>Picornaviridae Infections - epidemiology</subject><subject>Picornaviridae Infections - transmission</subject><subject>Picornaviridae Infections - veterinary</subject><subject>Picornaviridae Infections - virology</subject><subject>Porcine kobuvirus 1</subject><subject>RNA, Viral - genetics</subject><subject>Rotavirus</subject><subject>Rotavirus - classification</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - isolation & purification</subject><subject>Rotavirus Infections - epidemiology</subject><subject>Rotavirus Infections - transmission</subject><subject>Rotavirus Infections - veterinary</subject><subject>Rotavirus Infections - virology</subject><subject>Sequence Analysis, DNA</subject><subject>Severity of Illness Index</subject><subject>Slovakia - epidemiology</subject><subject>Swine</subject><subject>Swine Diseases - epidemiology</subject><subject>Swine Diseases - transmission</subject><subject>Swine Diseases - virology</subject><subject>Weaning</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQQC0EoqXwDxDykUsWT-KPLAekqoKCVAkOcLa89qSZZTdebKeonPnhuJuFI9JI9njezMiPsZcgViBAv9mu9oi3FFatALMSUAMesXNQ2jSmVebx6Q6d7M_Ys5y3ooKi7Z-ys7YXvZFrec5-f4nJ04T8e9zMd5TmzIHTxEd0uzLeczcFHsilNCJ5fqDb_JZf44SlZgEL-kJxOlJ-dMn5gol-ueNjHPgy8FiNDU3DCf9JZeQpFrfUL5-zJ4PbZXxxOi_Ytw_vv159bG4-X3-6urxpvBR9aXRopTebdu2Ccs4P0utgdA_eqI1yQ-gMSNkNeo2dWwsthFQelNLY1tQ40V2w18vcQ4o_ZszF7il73O3chHHOFnoNSnTaqIrKBfUp5pxwsIdEe5fuLQj74N9u7eLfPvi3AmpAbXt12jBv9hj-Nf0VXoF3C4D1n3eEyWZPOHkMlKodGyL9f8Mfv0maAw</recordid><startdate>201704</startdate><enddate>201704</enddate><creator>Jackova, Anna</creator><creator>Sliz, Ivan</creator><creator>Mandelik, Rene</creator><creator>Salamunova, Slavomira</creator><creator>Novotny, Jaroslav</creator><creator>Kolesarova, Mariana</creator><creator>Vlasakova, Michaela</creator><creator>Vilcek, Stefan</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201704</creationdate><title>Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A</title><author>Jackova, Anna ; Sliz, Ivan ; Mandelik, Rene ; Salamunova, Slavomira ; Novotny, Jaroslav ; Kolesarova, Mariana ; Vlasakova, Michaela ; Vilcek, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-6d24c7b29ad5aacf4c6d7681c75b5afd371443f69e3a9060045c1556e2a907a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Animals, Suckling</topic><topic>Asymptomatic Diseases</topic><topic>Cluster Analysis</topic><topic>Coinfection</topic><topic>Diarrhea</topic><topic>Diarrhea - epidemiology</topic><topic>Diarrhea - virology</topic><topic>Feces - virology</topic><topic>Kobuvirus - classification</topic><topic>Kobuvirus - genetics</topic><topic>Kobuvirus - isolation & purification</topic><topic>Phylogenetic analysis</topic><topic>Phylogeny</topic><topic>Picornaviridae Infections - epidemiology</topic><topic>Picornaviridae Infections - transmission</topic><topic>Picornaviridae Infections - veterinary</topic><topic>Picornaviridae Infections - virology</topic><topic>Porcine kobuvirus 1</topic><topic>RNA, Viral - genetics</topic><topic>Rotavirus</topic><topic>Rotavirus - classification</topic><topic>Rotavirus - genetics</topic><topic>Rotavirus - isolation & purification</topic><topic>Rotavirus Infections - epidemiology</topic><topic>Rotavirus Infections - transmission</topic><topic>Rotavirus Infections - veterinary</topic><topic>Rotavirus Infections - virology</topic><topic>Sequence Analysis, DNA</topic><topic>Severity of Illness Index</topic><topic>Slovakia - epidemiology</topic><topic>Swine</topic><topic>Swine Diseases - epidemiology</topic><topic>Swine Diseases - transmission</topic><topic>Swine Diseases - virology</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackova, Anna</creatorcontrib><creatorcontrib>Sliz, Ivan</creatorcontrib><creatorcontrib>Mandelik, Rene</creatorcontrib><creatorcontrib>Salamunova, Slavomira</creatorcontrib><creatorcontrib>Novotny, Jaroslav</creatorcontrib><creatorcontrib>Kolesarova, Mariana</creatorcontrib><creatorcontrib>Vlasakova, Michaela</creatorcontrib><creatorcontrib>Vilcek, Stefan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackova, Anna</au><au>Sliz, Ivan</au><au>Mandelik, Rene</au><au>Salamunova, Slavomira</au><au>Novotny, Jaroslav</au><au>Kolesarova, Mariana</au><au>Vlasakova, Michaela</au><au>Vilcek, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2017-04</date><risdate>2017</risdate><volume>49</volume><spage>73</spage><epage>77</epage><pages>73-77</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in Slovakia. Among all ages of animals, PKV-1 was detected equally in diarrheic (63.8%) and clinically healthy (62.9%) pigs. PKV-1 was more often detected in diarrheic (74.6%) than in healthy (64.4%) suckling piglets (<28days) but data was not statistically significant. Results in weaned (28–70days) and fattening (>70days) of both healthy and diarrheic pigs were inconsistent ranging in interval 56.2% to 67.9%. This study did not confirm a clear relationship of PKV-1 infection with diarrhea in pigs. Rotavirus A infection was detected among the same animals in 39% diarrheic and 9.2% healthy suckling piglets (p<0.001) confirming rotavirus as a causative agent of diarrhea in this age group. The difference was not significant in older pigs with both diarrheic and healthy pigs being infected within a range of 0% to 12.2%. Co-infection with PKV-1 and rotavirus A was detected overall in 5.6% of healthy and in 13.5% of diarrheic pigs and was highest in suckling piglets (33.9%). The PKV-1sequences from pigs in Slovakia were analyzed at the genetic level in the partial 3D gene region for the first time. The viral sequences were grouped in phylogenetic clusters according to their farm of origin. When compared with 157 nucleotide sequences originating from pig samples of different countries around the world Slovakian PKV-1 sequences were clustered in the phylogenetic tree with Asian sequences but not with nucleotide sequences from the neighbouring countries of Czech Republic or Hungary.
•The prevalence of PKV-1 and rotavirus A was studied in pigs of different age.•PKV-1 was equally detected in diarrheic (63.8%) and healthy (62.9%) pigs.•No clear relationship between detection of PKV-1 in pigs and diarrhea was confirmed.•Rotavirus A infection was associated significantly (p<0.001) with diarrhea in piglets.•Slovakian PKV-1 sequences were clustered in the phylogenetic tree with Asian sequences.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28087494</pmid><doi>10.1016/j.meegid.2017.01.011</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Suckling Asymptomatic Diseases Cluster Analysis Coinfection Diarrhea Diarrhea - epidemiology Diarrhea - virology Feces - virology Kobuvirus - classification Kobuvirus - genetics Kobuvirus - isolation & purification Phylogenetic analysis Phylogeny Picornaviridae Infections - epidemiology Picornaviridae Infections - transmission Picornaviridae Infections - veterinary Picornaviridae Infections - virology Porcine kobuvirus 1 RNA, Viral - genetics Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotavirus Infections - epidemiology Rotavirus Infections - transmission Rotavirus Infections - veterinary Rotavirus Infections - virology Sequence Analysis, DNA Severity of Illness Index Slovakia - epidemiology Swine Swine Diseases - epidemiology Swine Diseases - transmission Swine Diseases - virology Weaning |
| title | Porcine kobuvirus 1 in healthy and diarrheic pigs: Genetic detection and characterization of virus and co-infection with rotavirus A |
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