The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice
Social relations are built and maintained from the interaction among individuals. The oxytocin (OT), vasopressin (VP), estrogen, dopamine, and their receptors are involved in the modulation of sexual behavior in females. This study aimed to analyze the impact of OT gene knockout (OTKO) on sexual beh...
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| Veröffentlicht in: | Cellular and molecular neurobiology 2017-07, Vol.37 (5), p.803-815 |
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| creator | Zimmermann-Peruzatto, Josi Maria Lazzari, Virgínia Meneghini Agnes, Grasiela Becker, Roberta Oriques de Moura, Ana Carolina Guedes, Renata Padilha Lucion, Aldo Bolten Almeida, Silvana Giovenardi, Márcia |
| description | Social relations are built and maintained from the interaction among individuals. The oxytocin (OT), vasopressin (VP), estrogen, dopamine, and their receptors are involved in the modulation of sexual behavior in females. This study aimed to analyze the impact of OT gene knockout (OTKO) on sexual behavior and the gene expression of oxytocin (OTR), estrogen alpha (ERα), estrogen beta (ERβ), vasopressin (V
1a
R), and dopamine (D
2
R) receptors in the olfactory bulb (OB), prefrontal cortex (PFC), hippocampus (HPC), and hypothalamus (HPT), as well as in the synthesis of VP in the HPT of female mice. Wild-type (WT) littermates were used for comparisons. The
C
DNAs were synthesized by polymerase chain reaction and the gene expression was calculated with the 2
−ΔΔCt
formula. Our results showed that the absence of OT caused an increase in the frequency and duration of non-receptive postures and a decrease in receptive postures in the OTKO. OTKO females showed a significant decrease in the gene expression of OTR in the HPC, V
1a
R in the HPT, and ERα and ERβ in the PFC. There was no significant difference in the gene expression of D
2
R of OTKO. However, OTKO showed an increased gene expression of V
1a
R in the HPC. There is no significant difference in VP mRNA synthesis in the HPT between OTKO and WT. Our findings demonstrate that the absence of OT leads to significant changes in the expression of the studied genes (OTR, ERα, ERβ, V
1a
R), and these changes may contribute to the decreased sexual behavior observed in OTKO females. |
| doi_str_mv | 10.1007/s10571-016-0419-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859732381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1899834210</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-48c416812783b885e54d986a6346df7464e10242f0f18212ee40464cd3d2b76e3</originalsourceid><addsrcrecordid>eNp1kc1u1DAURi0EokPLA7BBltiwSbn-i50lrdpStVCJtmvL49zQlMSe2gmaeQTeGlcpCCGxsuV77vdZOoS8YXDIAPSHzEBpVgGrK5CsqcQzsmJKi6o2Ap6TFXDNKykk7JFXOd8DQAOgXpI9rpUyWqgV-Xlzh_R83Dg_0djRq-1uir4P9AwD0osQ_fc4l0mg17id3UCP8M796GOiLrQLdLLdJMy5L8xXHNyELZ0i_YJzihja6FNfoOtdnnDMtCRPpfAouXIrfac4ugHp597jAXnRuSHj66dzn9yentwcf6our87Ojz9eVl5oPlXSeMlqw7g2Ym2MQiXbxtSuFrJuOy1riQy45B10zHDGESWUR9-Klq91jWKfvF9yNyk-zJgnO_bZ4zC4gHHOlhnVaMGFYQV99w96H-cUyu8K1TRGSM6gUGyhfIo5J-zsJvWjSzvLwD56sosnWzzZR09WlJ23T8nzesT2z8ZvMQXgC5DLKHzD9Ff1f1N_AeW8nAQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1899834210</pqid></control><display><type>article</type><title>The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Zimmermann-Peruzatto, Josi Maria ; Lazzari, Virgínia Meneghini ; Agnes, Grasiela ; Becker, Roberta Oriques ; de Moura, Ana Carolina ; Guedes, Renata Padilha ; Lucion, Aldo Bolten ; Almeida, Silvana ; Giovenardi, Márcia</creator><creatorcontrib>Zimmermann-Peruzatto, Josi Maria ; Lazzari, Virgínia Meneghini ; Agnes, Grasiela ; Becker, Roberta Oriques ; de Moura, Ana Carolina ; Guedes, Renata Padilha ; Lucion, Aldo Bolten ; Almeida, Silvana ; Giovenardi, Márcia</creatorcontrib><description>Social relations are built and maintained from the interaction among individuals. The oxytocin (OT), vasopressin (VP), estrogen, dopamine, and their receptors are involved in the modulation of sexual behavior in females. This study aimed to analyze the impact of OT gene knockout (OTKO) on sexual behavior and the gene expression of oxytocin (OTR), estrogen alpha (ERα), estrogen beta (ERβ), vasopressin (V
1a
R), and dopamine (D
2
R) receptors in the olfactory bulb (OB), prefrontal cortex (PFC), hippocampus (HPC), and hypothalamus (HPT), as well as in the synthesis of VP in the HPT of female mice. Wild-type (WT) littermates were used for comparisons. The
C
DNAs were synthesized by polymerase chain reaction and the gene expression was calculated with the 2
−ΔΔCt
formula. Our results showed that the absence of OT caused an increase in the frequency and duration of non-receptive postures and a decrease in receptive postures in the OTKO. OTKO females showed a significant decrease in the gene expression of OTR in the HPC, V
1a
R in the HPT, and ERα and ERβ in the PFC. There was no significant difference in the gene expression of D
2
R of OTKO. However, OTKO showed an increased gene expression of V
1a
R in the HPC. There is no significant difference in VP mRNA synthesis in the HPT between OTKO and WT. Our findings demonstrate that the absence of OT leads to significant changes in the expression of the studied genes (OTR, ERα, ERβ, V
1a
R), and these changes may contribute to the decreased sexual behavior observed in OTKO females.</description><identifier>ISSN: 0272-4340</identifier><identifier>ISSN: 1573-6830</identifier><identifier>EISSN: 1573-6830</identifier><identifier>DOI: 10.1007/s10571-016-0419-3</identifier><identifier>PMID: 27558735</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Argipressin receptors ; Biomedical and Life Sciences ; Biomedicine ; Brain - metabolism ; Cell Biology ; Cortex (olfactory) ; Dopamine ; Dopamine D2 receptors ; Female ; Females ; Gene expression ; Gene Expression Regulation ; Gene Knockout Techniques ; Hypothalamus ; Mice, Inbred C57BL ; Mice, Knockout ; Neurobiology ; Neuroendocrine system ; Neurosciences ; Neurosecretory Systems - metabolism ; Olfactory bulb ; Original Research ; Oxytocin ; Oxytocin - genetics ; Oxytocin - metabolism ; Polymerase chain reaction ; Prefrontal cortex ; Receptors, Dopamine - genetics ; Receptors, Dopamine - metabolism ; Receptors, Estrogen - genetics ; Receptors, Estrogen - metabolism ; Receptors, Vasopressin - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Sexual Behavior ; Transcription ; Vasopressin ; Vasopressins - genetics ; Vasopressins - metabolism</subject><ispartof>Cellular and molecular neurobiology, 2017-07, Vol.37 (5), p.803-815</ispartof><rights>Springer Science+Business Media New York 2016</rights><rights>Copyright Springer Science & Business Media 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-48c416812783b885e54d986a6346df7464e10242f0f18212ee40464cd3d2b76e3</citedby><cites>FETCH-LOGICAL-c372t-48c416812783b885e54d986a6346df7464e10242f0f18212ee40464cd3d2b76e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10571-016-0419-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10571-016-0419-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27558735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zimmermann-Peruzatto, Josi Maria</creatorcontrib><creatorcontrib>Lazzari, Virgínia Meneghini</creatorcontrib><creatorcontrib>Agnes, Grasiela</creatorcontrib><creatorcontrib>Becker, Roberta Oriques</creatorcontrib><creatorcontrib>de Moura, Ana Carolina</creatorcontrib><creatorcontrib>Guedes, Renata Padilha</creatorcontrib><creatorcontrib>Lucion, Aldo Bolten</creatorcontrib><creatorcontrib>Almeida, Silvana</creatorcontrib><creatorcontrib>Giovenardi, Márcia</creatorcontrib><title>The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice</title><title>Cellular and molecular neurobiology</title><addtitle>Cell Mol Neurobiol</addtitle><addtitle>Cell Mol Neurobiol</addtitle><description>Social relations are built and maintained from the interaction among individuals. The oxytocin (OT), vasopressin (VP), estrogen, dopamine, and their receptors are involved in the modulation of sexual behavior in females. This study aimed to analyze the impact of OT gene knockout (OTKO) on sexual behavior and the gene expression of oxytocin (OTR), estrogen alpha (ERα), estrogen beta (ERβ), vasopressin (V
1a
R), and dopamine (D
2
R) receptors in the olfactory bulb (OB), prefrontal cortex (PFC), hippocampus (HPC), and hypothalamus (HPT), as well as in the synthesis of VP in the HPT of female mice. Wild-type (WT) littermates were used for comparisons. The
C
DNAs were synthesized by polymerase chain reaction and the gene expression was calculated with the 2
−ΔΔCt
formula. Our results showed that the absence of OT caused an increase in the frequency and duration of non-receptive postures and a decrease in receptive postures in the OTKO. OTKO females showed a significant decrease in the gene expression of OTR in the HPC, V
1a
R in the HPT, and ERα and ERβ in the PFC. There was no significant difference in the gene expression of D
2
R of OTKO. However, OTKO showed an increased gene expression of V
1a
R in the HPC. There is no significant difference in VP mRNA synthesis in the HPT between OTKO and WT. Our findings demonstrate that the absence of OT leads to significant changes in the expression of the studied genes (OTR, ERα, ERβ, V
1a
R), and these changes may contribute to the decreased sexual behavior observed in OTKO females.</description><subject>Animals</subject><subject>Argipressin receptors</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain - metabolism</subject><subject>Cell Biology</subject><subject>Cortex (olfactory)</subject><subject>Dopamine</subject><subject>Dopamine D2 receptors</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene Knockout Techniques</subject><subject>Hypothalamus</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Neurobiology</subject><subject>Neuroendocrine system</subject><subject>Neurosciences</subject><subject>Neurosecretory Systems - metabolism</subject><subject>Olfactory bulb</subject><subject>Original Research</subject><subject>Oxytocin</subject><subject>Oxytocin - genetics</subject><subject>Oxytocin - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Prefrontal cortex</subject><subject>Receptors, Dopamine - genetics</subject><subject>Receptors, Dopamine - metabolism</subject><subject>Receptors, Estrogen - genetics</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Receptors, Vasopressin - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Rodents</subject><subject>Sexual Behavior</subject><subject>Transcription</subject><subject>Vasopressin</subject><subject>Vasopressins - genetics</subject><subject>Vasopressins - metabolism</subject><issn>0272-4340</issn><issn>1573-6830</issn><issn>1573-6830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAURi0EokPLA7BBltiwSbn-i50lrdpStVCJtmvL49zQlMSe2gmaeQTeGlcpCCGxsuV77vdZOoS8YXDIAPSHzEBpVgGrK5CsqcQzsmJKi6o2Ap6TFXDNKykk7JFXOd8DQAOgXpI9rpUyWqgV-Xlzh_R83Dg_0djRq-1uir4P9AwD0osQ_fc4l0mg17id3UCP8M796GOiLrQLdLLdJMy5L8xXHNyELZ0i_YJzihja6FNfoOtdnnDMtCRPpfAouXIrfac4ugHp597jAXnRuSHj66dzn9yentwcf6our87Ojz9eVl5oPlXSeMlqw7g2Ym2MQiXbxtSuFrJuOy1riQy45B10zHDGESWUR9-Klq91jWKfvF9yNyk-zJgnO_bZ4zC4gHHOlhnVaMGFYQV99w96H-cUyu8K1TRGSM6gUGyhfIo5J-zsJvWjSzvLwD56sosnWzzZR09WlJ23T8nzesT2z8ZvMQXgC5DLKHzD9Ff1f1N_AeW8nAQ</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Zimmermann-Peruzatto, Josi Maria</creator><creator>Lazzari, Virgínia Meneghini</creator><creator>Agnes, Grasiela</creator><creator>Becker, Roberta Oriques</creator><creator>de Moura, Ana Carolina</creator><creator>Guedes, Renata Padilha</creator><creator>Lucion, Aldo Bolten</creator><creator>Almeida, Silvana</creator><creator>Giovenardi, Márcia</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170701</creationdate><title>The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice</title><author>Zimmermann-Peruzatto, Josi Maria ; Lazzari, Virgínia Meneghini ; Agnes, Grasiela ; Becker, Roberta Oriques ; de Moura, Ana Carolina ; Guedes, Renata Padilha ; Lucion, Aldo Bolten ; Almeida, Silvana ; Giovenardi, Márcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-48c416812783b885e54d986a6346df7464e10242f0f18212ee40464cd3d2b76e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Argipressin receptors</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain - metabolism</topic><topic>Cell Biology</topic><topic>Cortex (olfactory)</topic><topic>Dopamine</topic><topic>Dopamine D2 receptors</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene Knockout Techniques</topic><topic>Hypothalamus</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Neurobiology</topic><topic>Neuroendocrine system</topic><topic>Neurosciences</topic><topic>Neurosecretory Systems - metabolism</topic><topic>Olfactory bulb</topic><topic>Original Research</topic><topic>Oxytocin</topic><topic>Oxytocin - genetics</topic><topic>Oxytocin - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Prefrontal cortex</topic><topic>Receptors, Dopamine - genetics</topic><topic>Receptors, Dopamine - metabolism</topic><topic>Receptors, Estrogen - genetics</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Receptors, Vasopressin - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Rodents</topic><topic>Sexual Behavior</topic><topic>Transcription</topic><topic>Vasopressin</topic><topic>Vasopressins - genetics</topic><topic>Vasopressins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zimmermann-Peruzatto, Josi Maria</creatorcontrib><creatorcontrib>Lazzari, Virgínia Meneghini</creatorcontrib><creatorcontrib>Agnes, Grasiela</creatorcontrib><creatorcontrib>Becker, Roberta Oriques</creatorcontrib><creatorcontrib>de Moura, Ana Carolina</creatorcontrib><creatorcontrib>Guedes, Renata Padilha</creatorcontrib><creatorcontrib>Lucion, Aldo Bolten</creatorcontrib><creatorcontrib>Almeida, Silvana</creatorcontrib><creatorcontrib>Giovenardi, Márcia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular and molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zimmermann-Peruzatto, Josi Maria</au><au>Lazzari, Virgínia Meneghini</au><au>Agnes, Grasiela</au><au>Becker, Roberta Oriques</au><au>de Moura, Ana Carolina</au><au>Guedes, Renata Padilha</au><au>Lucion, Aldo Bolten</au><au>Almeida, Silvana</au><au>Giovenardi, Márcia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice</atitle><jtitle>Cellular and molecular neurobiology</jtitle><stitle>Cell Mol Neurobiol</stitle><addtitle>Cell Mol Neurobiol</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>37</volume><issue>5</issue><spage>803</spage><epage>815</epage><pages>803-815</pages><issn>0272-4340</issn><issn>1573-6830</issn><eissn>1573-6830</eissn><abstract>Social relations are built and maintained from the interaction among individuals. The oxytocin (OT), vasopressin (VP), estrogen, dopamine, and their receptors are involved in the modulation of sexual behavior in females. This study aimed to analyze the impact of OT gene knockout (OTKO) on sexual behavior and the gene expression of oxytocin (OTR), estrogen alpha (ERα), estrogen beta (ERβ), vasopressin (V
1a
R), and dopamine (D
2
R) receptors in the olfactory bulb (OB), prefrontal cortex (PFC), hippocampus (HPC), and hypothalamus (HPT), as well as in the synthesis of VP in the HPT of female mice. Wild-type (WT) littermates were used for comparisons. The
C
DNAs were synthesized by polymerase chain reaction and the gene expression was calculated with the 2
−ΔΔCt
formula. Our results showed that the absence of OT caused an increase in the frequency and duration of non-receptive postures and a decrease in receptive postures in the OTKO. OTKO females showed a significant decrease in the gene expression of OTR in the HPC, V
1a
R in the HPT, and ERα and ERβ in the PFC. There was no significant difference in the gene expression of D
2
R of OTKO. However, OTKO showed an increased gene expression of V
1a
R in the HPC. There is no significant difference in VP mRNA synthesis in the HPT between OTKO and WT. Our findings demonstrate that the absence of OT leads to significant changes in the expression of the studied genes (OTR, ERα, ERβ, V
1a
R), and these changes may contribute to the decreased sexual behavior observed in OTKO females.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27558735</pmid><doi>10.1007/s10571-016-0419-3</doi><tpages>13</tpages></addata></record> |
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| ispartof | Cellular and molecular neurobiology, 2017-07, Vol.37 (5), p.803-815 |
| issn | 0272-4340 1573-6830 1573-6830 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Argipressin receptors Biomedical and Life Sciences Biomedicine Brain - metabolism Cell Biology Cortex (olfactory) Dopamine Dopamine D2 receptors Female Females Gene expression Gene Expression Regulation Gene Knockout Techniques Hypothalamus Mice, Inbred C57BL Mice, Knockout Neurobiology Neuroendocrine system Neurosciences Neurosecretory Systems - metabolism Olfactory bulb Original Research Oxytocin Oxytocin - genetics Oxytocin - metabolism Polymerase chain reaction Prefrontal cortex Receptors, Dopamine - genetics Receptors, Dopamine - metabolism Receptors, Estrogen - genetics Receptors, Estrogen - metabolism Receptors, Vasopressin - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Rodents Sexual Behavior Transcription Vasopressin Vasopressins - genetics Vasopressins - metabolism |
| title | The Impact of Oxytocin Gene Knockout on Sexual Behavior and Gene Expression Related to Neuroendocrine Systems in the Brain of Female Mice |
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