Preparation of an improved hydrophilic monolith to make trypsin-immobilized microreactors

•Highly hydrophilic HEMA-MBA monoliths prepared with high monomer content reduce protein adsorption.•HEMA-MBA monoliths with new porogen composition allow high density of functional groups for trypsin immobilization.•Trypsin-immobilized microreactors show excellent homogeneity across 8cm based on hy...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-02, Vol.1043, p.128-137
Hauptverfasser:	Meller, Kinga, Pomastowski, Paweł, Szumski, Michał, Buszewski, Bogusław
Format:	Artikel
Sprache:	eng
Schlagworte:	2-Hydroxyethyl methacrylate Acrylamides - chemistry Animals Bioreactors Cattle Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Immobilized enzyme microreactor Mass Spectrometry Methacrylates - chemistry Monolithic bed N,N′-Methylenebis(acrylamide) Peptide Fragments - analysis Peptide Fragments - chemistry Peptide Fragments - metabolism Proteomics Proteomics - methods Serum Albumin, Bovine - analysis Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - metabolism Trypsin Trypsin - chemistry Trypsin - metabolism
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