Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases
The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria...
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| Veröffentlicht in: | FEMS microbiology letters 2016-09, Vol.363 (18), p.fnw193 |
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| creator | Neguţ, Alina Cristina Chifiriuc, Mariana-Carmen Săndulescu, Oana Streinu-Cercel, Anca Oprea, Mihaela Drăgulescu, Elena Carmina Gheorghe, Irina Berciu, Ioana Coralia, Bleotu Popa, Marcela Oţelea, Dan Tălăpan, Daniela Dorobăţ, Olga Codiţă, Irina Popa, Mircea Ioan Streinu-Cercel, Adrian |
| description | The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria. We have assessed the influence of commercially available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages: 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9-fold for STAPHYLOCOCCAL; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations (P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially available bacteriophages. We have also ascertained there is phage-driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections.
Commercially available bacteriophage cocktails inhibit biofilm formation by Staphylococcus strains. |
| doi_str_mv | 10.1093/femsle/fnw193 |
| format | Article |
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Commercially available bacteriophage cocktails inhibit biofilm formation by Staphylococcus strains.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnw193</identifier><identifier>PMID: 27521261</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Biofilms ; Depolymerization ; Infectious diseases ; Lysis ; Methicillin ; Microbiology ; Phages ; Prostheses ; Staphylococcus ; Strains (organisms) ; Virulence</subject><ispartof>FEMS microbiology letters, 2016-09, Vol.363 (18), p.fnw193</ispartof><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2016</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-1d99f1cba2d2d5b85bba80592762c83123ffc90018a8bfe4d6af48127d6046eb3</citedby><cites>FETCH-LOGICAL-c354t-1d99f1cba2d2d5b85bba80592762c83123ffc90018a8bfe4d6af48127d6046eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27521261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Millard, Andrew</contributor><creatorcontrib>Neguţ, Alina Cristina</creatorcontrib><creatorcontrib>Chifiriuc, Mariana-Carmen</creatorcontrib><creatorcontrib>Săndulescu, Oana</creatorcontrib><creatorcontrib>Streinu-Cercel, Anca</creatorcontrib><creatorcontrib>Oprea, Mihaela</creatorcontrib><creatorcontrib>Drăgulescu, Elena Carmina</creatorcontrib><creatorcontrib>Gheorghe, Irina</creatorcontrib><creatorcontrib>Berciu, Ioana</creatorcontrib><creatorcontrib>Coralia, Bleotu</creatorcontrib><creatorcontrib>Popa, Marcela</creatorcontrib><creatorcontrib>Oţelea, Dan</creatorcontrib><creatorcontrib>Tălăpan, Daniela</creatorcontrib><creatorcontrib>Dorobăţ, Olga</creatorcontrib><creatorcontrib>Codiţă, Irina</creatorcontrib><creatorcontrib>Popa, Mircea Ioan</creatorcontrib><creatorcontrib>Streinu-Cercel, Adrian</creatorcontrib><title>Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria. We have assessed the influence of commercially available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages: 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9-fold for STAPHYLOCOCCAL; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations (P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially available bacteriophages. We have also ascertained there is phage-driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections.
Commercially available bacteriophage cocktails inhibit biofilm formation by Staphylococcus strains.</description><subject>Biofilms</subject><subject>Depolymerization</subject><subject>Infectious diseases</subject><subject>Lysis</subject><subject>Methicillin</subject><subject>Microbiology</subject><subject>Phages</subject><subject>Prostheses</subject><subject>Staphylococcus</subject><subject>Strains 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Bleotu</au><au>Popa, Marcela</au><au>Oţelea, Dan</au><au>Tălăpan, Daniela</au><au>Dorobăţ, Olga</au><au>Codiţă, Irina</au><au>Popa, Mircea Ioan</au><au>Streinu-Cercel, Adrian</au><au>Millard, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>363</volume><issue>18</issue><spage>fnw193</spage><pages>fnw193-</pages><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>The increasing burden of invasive biofilm-related staphylococcal infections has led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter a biofilm through several mechanisms, including induction of depolymerizing enzymes and lysis of persistent bacteria. We have assessed the influence of commercially available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages: 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9-fold for STAPHYLOCOCCAL; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations (P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially available bacteriophages. We have also ascertained there is phage-driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections.
Commercially available bacteriophage cocktails inhibit biofilm formation by Staphylococcus strains.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27521261</pmid><doi>10.1093/femsle/fnw193</doi></addata></record> |
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| identifier | ISSN: 1574-6968 |
| ispartof | FEMS microbiology letters, 2016-09, Vol.363 (18), p.fnw193 |
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| source | Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Biofilms Depolymerization Infectious diseases Lysis Methicillin Microbiology Phages Prostheses Staphylococcus Strains (organisms) Virulence |
| title | Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases |
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