Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice
Previously, we demonstrated that Lactobacillus salivarius was more abundant in patients with rheumatoid arthritis (RA), an inflammatory autoimmune disease wherein the gut microbiota is altered, than in healthy individuals. However, the effect of L. salivarius in RA is unclear. Hence, we investigated...
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| Veröffentlicht in: | Journal of interferon & cytokine research 2016-12, Vol.36 (12), p.706-712 |
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| creator | Liu, Xiaofei Zhang, Juan Zou, Qinghua Zhong, Bing Wang, Heng Mou, Fangxiang Wu, Like Fang, Yongfei |
| description | Previously, we demonstrated that Lactobacillus salivarius was more abundant in patients with rheumatoid arthritis (RA), an inflammatory autoimmune disease wherein the gut microbiota is altered, than in healthy individuals. However, the effect of L. salivarius in RA is unclear. Hence, we investigated the effect of L. salivarius isolated from patients with RA on collagen-induced arthritis (CIA) in mice. L. salivarius UCC118 or L. plantarum WCFS1 isolated from patients with RA was administered orally for 5 weeks, starting from 2 weeks before the induction of arthritis in DBA/1 mice. Clinical score progression, histological changes, serum cytokine concentrations, and the proportion of interleukin (IL)-17-producing T cells [T helper 17 (Th17)] and regulatory T cells (Tregs) in the spleen were evaluated. Bone erosion was evaluated by micro-computed tomography. CIA mice treated with either L. salivarius or L. plantarum showed lower arthritis scores, milder synovial infiltration, and less bone erosion when compared with phosphate-buffered, saline-treated CIA mice. Administration of L. salivarius and L. plantarum reduced the Th17 cell fraction and increased the Treg fraction. L. salivarius-treated CIA mice displayed a significant increase in serum anti-inflammatory IL-10 levels. Thus, pretreatment with L. salivarius could significantly improve CIA in mice and may help alleviate RA in a clinical setting. |
| doi_str_mv | 10.1089/jir.2016.0057 |
| format | Article |
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However, the effect of L. salivarius in RA is unclear. Hence, we investigated the effect of L. salivarius isolated from patients with RA on collagen-induced arthritis (CIA) in mice. L. salivarius UCC118 or L. plantarum WCFS1 isolated from patients with RA was administered orally for 5 weeks, starting from 2 weeks before the induction of arthritis in DBA/1 mice. Clinical score progression, histological changes, serum cytokine concentrations, and the proportion of interleukin (IL)-17-producing T cells [T helper 17 (Th17)] and regulatory T cells (Tregs) in the spleen were evaluated. Bone erosion was evaluated by micro-computed tomography. CIA mice treated with either L. salivarius or L. plantarum showed lower arthritis scores, milder synovial infiltration, and less bone erosion when compared with phosphate-buffered, saline-treated CIA mice. Administration of L. salivarius and L. plantarum reduced the Th17 cell fraction and increased the Treg fraction. L. salivarius-treated CIA mice displayed a significant increase in serum anti-inflammatory IL-10 levels. Thus, pretreatment with L. salivarius could significantly improve CIA in mice and may help alleviate RA in a clinical setting.</description><identifier>ISSN: 1079-9907</identifier><identifier>EISSN: 1557-7465</identifier><identifier>DOI: 10.1089/jir.2016.0057</identifier><identifier>PMID: 27845855</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Arthritis, Experimental ; Arthritis, Rheumatoid - blood ; Arthritis, Rheumatoid - diagnosis ; Arthritis, Rheumatoid - immunology ; Arthritis, Rheumatoid - therapy ; Biopsy ; Cytokines - blood ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Humans ; Inflammation Mediators ; Lactobacillus salivarius ; Lactobacillus salivarius - genetics ; Lactobacillus salivarius - immunology ; Lactobacillus salivarius - isolation & purification ; Lymphocyte Count ; Mice ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; Th17 Cells - immunology ; Th17 Cells - metabolism ; X-Ray Microtomography</subject><ispartof>Journal of interferon & cytokine research, 2016-12, Vol.36 (12), p.706-712</ispartof><rights>(©) Copyright 2016, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-d6e0ad4eee8081f5abe393418ea3cf18297da3c1900643f08fbf7da1547c6bf53</citedby><cites>FETCH-LOGICAL-c354t-d6e0ad4eee8081f5abe393418ea3cf18297da3c1900643f08fbf7da1547c6bf53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27845855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiaofei</creatorcontrib><creatorcontrib>Zhang, Juan</creatorcontrib><creatorcontrib>Zou, Qinghua</creatorcontrib><creatorcontrib>Zhong, Bing</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Mou, Fangxiang</creatorcontrib><creatorcontrib>Wu, Like</creatorcontrib><creatorcontrib>Fang, Yongfei</creatorcontrib><title>Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice</title><title>Journal of interferon & cytokine research</title><addtitle>J Interferon Cytokine Res</addtitle><description>Previously, we demonstrated that Lactobacillus salivarius was more abundant in patients with rheumatoid arthritis (RA), an inflammatory autoimmune disease wherein the gut microbiota is altered, than in healthy individuals. However, the effect of L. salivarius in RA is unclear. Hence, we investigated the effect of L. salivarius isolated from patients with RA on collagen-induced arthritis (CIA) in mice. L. salivarius UCC118 or L. plantarum WCFS1 isolated from patients with RA was administered orally for 5 weeks, starting from 2 weeks before the induction of arthritis in DBA/1 mice. Clinical score progression, histological changes, serum cytokine concentrations, and the proportion of interleukin (IL)-17-producing T cells [T helper 17 (Th17)] and regulatory T cells (Tregs) in the spleen were evaluated. Bone erosion was evaluated by micro-computed tomography. CIA mice treated with either L. salivarius or L. plantarum showed lower arthritis scores, milder synovial infiltration, and less bone erosion when compared with phosphate-buffered, saline-treated CIA mice. Administration of L. salivarius and L. plantarum reduced the Th17 cell fraction and increased the Treg fraction. L. salivarius-treated CIA mice displayed a significant increase in serum anti-inflammatory IL-10 levels. Thus, pretreatment with L. salivarius could significantly improve CIA in mice and may help alleviate RA in a clinical setting.</description><subject>Animals</subject><subject>Arthritis, Experimental</subject><subject>Arthritis, Rheumatoid - blood</subject><subject>Arthritis, Rheumatoid - diagnosis</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Arthritis, Rheumatoid - therapy</subject><subject>Biopsy</subject><subject>Cytokines - blood</subject><subject>Disease Models, Animal</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Inflammation Mediators</subject><subject>Lactobacillus salivarius</subject><subject>Lactobacillus salivarius - genetics</subject><subject>Lactobacillus salivarius - immunology</subject><subject>Lactobacillus salivarius - isolation & purification</subject><subject>Lymphocyte Count</subject><subject>Mice</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Th17 Cells - immunology</subject><subject>Th17 Cells - metabolism</subject><subject>X-Ray Microtomography</subject><issn>1079-9907</issn><issn>1557-7465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkUFrHCEYhqWkNGnaY65FyKWX2eqqox7DkrQLW1ra9Dw4zmfWZUY36iTkf_QH1yVpKDn15Is8fHzv9yB0RsmCEqU_7XxaLAltF4QI-QqdUCFkI3krjmomUjdaE3mM3ua8I4S0aqnfoOOlVFwoIU7Q742xJfbG-nGcM85m9Hcm-RrXOY6mwIBdihP-boqHUDK-92WLf2xhnkyJfsAXqWyTLz7jn_N-nyBnyHgVx9HcQGjWYZgt_EuZMOB1sAnMAbxOcIOvEtzOEOwD9gF_9RbeodfOjBneP72n6NfV5fXqS7P59nm9utg0lglemqEFYgYOAIoo6oTpgWnGqQLDrKO1qhxqorr25swR5XpXf6jg0ra9E-wUfXycu0-xbpBLN_lsoe4eIM65o0porsWSsf9AmZasbTmp6PkLdBfnFGqRStWzt5pQWqnmkbIp5pzAdfvkJ5MeOkq6g9mumu0OZruD2cp_eJo69xMMz_RflewPn2Chdw</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Liu, Xiaofei</creator><creator>Zhang, Juan</creator><creator>Zou, Qinghua</creator><creator>Zhong, Bing</creator><creator>Wang, Heng</creator><creator>Mou, Fangxiang</creator><creator>Wu, Like</creator><creator>Fang, Yongfei</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice</title><author>Liu, Xiaofei ; Zhang, Juan ; Zou, Qinghua ; Zhong, Bing ; Wang, Heng ; Mou, Fangxiang ; Wu, Like ; Fang, Yongfei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-d6e0ad4eee8081f5abe393418ea3cf18297da3c1900643f08fbf7da1547c6bf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Arthritis, Experimental</topic><topic>Arthritis, Rheumatoid - blood</topic><topic>Arthritis, Rheumatoid - diagnosis</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Arthritis, Rheumatoid - therapy</topic><topic>Biopsy</topic><topic>Cytokines - blood</topic><topic>Disease Models, Animal</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Inflammation Mediators</topic><topic>Lactobacillus salivarius</topic><topic>Lactobacillus salivarius - genetics</topic><topic>Lactobacillus salivarius - immunology</topic><topic>Lactobacillus salivarius - isolation & purification</topic><topic>Lymphocyte Count</topic><topic>Mice</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>Th17 Cells - immunology</topic><topic>Th17 Cells - metabolism</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiaofei</creatorcontrib><creatorcontrib>Zhang, Juan</creatorcontrib><creatorcontrib>Zou, Qinghua</creatorcontrib><creatorcontrib>Zhong, Bing</creatorcontrib><creatorcontrib>Wang, Heng</creatorcontrib><creatorcontrib>Mou, Fangxiang</creatorcontrib><creatorcontrib>Wu, Like</creatorcontrib><creatorcontrib>Fang, Yongfei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of interferon & cytokine research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiaofei</au><au>Zhang, Juan</au><au>Zou, Qinghua</au><au>Zhong, Bing</au><au>Wang, Heng</au><au>Mou, Fangxiang</au><au>Wu, Like</au><au>Fang, Yongfei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice</atitle><jtitle>Journal of interferon & cytokine research</jtitle><addtitle>J Interferon Cytokine Res</addtitle><date>2016-12</date><risdate>2016</risdate><volume>36</volume><issue>12</issue><spage>706</spage><epage>712</epage><pages>706-712</pages><issn>1079-9907</issn><eissn>1557-7465</eissn><abstract>Previously, we demonstrated that Lactobacillus salivarius was more abundant in patients with rheumatoid arthritis (RA), an inflammatory autoimmune disease wherein the gut microbiota is altered, than in healthy individuals. However, the effect of L. salivarius in RA is unclear. Hence, we investigated the effect of L. salivarius isolated from patients with RA on collagen-induced arthritis (CIA) in mice. L. salivarius UCC118 or L. plantarum WCFS1 isolated from patients with RA was administered orally for 5 weeks, starting from 2 weeks before the induction of arthritis in DBA/1 mice. Clinical score progression, histological changes, serum cytokine concentrations, and the proportion of interleukin (IL)-17-producing T cells [T helper 17 (Th17)] and regulatory T cells (Tregs) in the spleen were evaluated. Bone erosion was evaluated by micro-computed tomography. CIA mice treated with either L. salivarius or L. plantarum showed lower arthritis scores, milder synovial infiltration, and less bone erosion when compared with phosphate-buffered, saline-treated CIA mice. Administration of L. salivarius and L. plantarum reduced the Th17 cell fraction and increased the Treg fraction. L. salivarius-treated CIA mice displayed a significant increase in serum anti-inflammatory IL-10 levels. Thus, pretreatment with L. salivarius could significantly improve CIA in mice and may help alleviate RA in a clinical setting.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>27845855</pmid><doi>10.1089/jir.2016.0057</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Arthritis, Experimental Arthritis, Rheumatoid - blood Arthritis, Rheumatoid - diagnosis Arthritis, Rheumatoid - immunology Arthritis, Rheumatoid - therapy Biopsy Cytokines - blood Disease Models, Animal Enzyme-Linked Immunosorbent Assay Flow Cytometry Humans Inflammation Mediators Lactobacillus salivarius Lactobacillus salivarius - genetics Lactobacillus salivarius - immunology Lactobacillus salivarius - isolation & purification Lymphocyte Count Mice T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism Th17 Cells - immunology Th17 Cells - metabolism X-Ray Microtomography |
| title | Lactobacillus salivarius Isolated from Patients with Rheumatoid Arthritis Suppresses Collagen-Induced Arthritis and Increases Treg Frequency in Mice |
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