Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens
Aim This study assessed whether multilocus variable‐number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people. Methods and Results MLVA geno...
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| Veröffentlicht in: | Journal of applied microbiology 2017-01, Vol.122 (1), p.268-278 |
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| container_title | Journal of applied microbiology |
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| creator | Wang, L. Nakamura, H. Kage‐Nakadai, E. Hara‐Kudo, Y. Nishikawa, Y. |
| description | Aim
This study assessed whether multilocus variable‐number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people.
Methods and Results
MLVA genotyping of 142 aEPEC strains isolated from foods and faecal samples of domestic animals and humans revealed 126 distinct MLVA profiles that distributed to four clusters, yielding a Simpson's index of diversity (D) of 99·8%. Cluster 2 included 87% of cattle isolates and 67% of patient isolates. The plurality (15/34, 44%) of strains from healthy humans mapped to Cluster 1, while half (18/41, 44%) of the swine strains belonged to Cluster 4. Testing for antimicrobial susceptibility revealed that 52 strains (37%) of aEPEC were resistant to one or more agents; only 10 strains (7%) exhibited resistance to more than three agents. Strains isolated from swine or food exhibited a wider variety of resistance phenotypes than bovine or human strains.
Conclusions
MLVA assigned the aEPEC isolates from cattle and patients to Cluster 2, distinct from aEPEC from other sources. Hog yards may be a larger source of drug‐resistant strains than are cattle ranches.
Significance and Impact of the Study
MLVA suggests that human diarrhoeagenic aEPEC are derived from cattle and are distinct from strains carried by healthy people and other animals. Cattle appear to be reservoirs of human diarrhoeagenic aEPEC. |
| doi_str_mv | 10.1111/jam.13322 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859487808</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4286574101</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4302-5733d8a3134dece792893cdd5603bd17e6d3cbf9026a3626708a4461e572c5293</originalsourceid><addsrcrecordid>eNqNksuKFDEYhQtRnHF04QtIwI0uaiaXSl2WQzPeGHGj6-Kv5K_pNLmUSZXSOx_Bx3Ptk5jual0IgoGQn-TjnHA4RfGU0UuW19UO3CUTgvN7xTkTtSx53fD7x7kqJW34WfEopR2lTFBZPyzOeNOwVjB5XvzYBDdBNCl4MuyJW-xsbFBLIl_yLQwWf3777hc3YCQzeI2ORJwQZgIe7D6ZlAed92ycUTEMBmwm8n2mFRJwwd8RmPeTUfkF_YwxTDBvwx16o8hNUluMRm0NEBWsIWmOYHwi-UcWZtRkjMGRMQRNErjJ4mq4XRz4k7VxWXkEPBikCZVx6NPj4sEINuGT03lRfHp183Hzprz98Prt5vq2VJWgvJSNELoFwUSlUWHT8bYTSmtZUzFo1mCthRrGjvIaRJ1zpS1UVc1QNlxJ3omL4sWqO8XwecE0984khdaCx7CknrWyq9qmpe1_oEKKtu6EyOjzv9BdWGJO_CjIGyqZPHi_XKkcfEoRx36KOYy47xntD83oczP6YzMy--ykuAwO9R_ydxUycLUCX43F_b-V-nfX71fJXzxGyAw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1852705159</pqid></control><display><type>article</type><title>Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens</title><source>MEDLINE</source><source>Wiley Online Library Journals</source><source>Oxford Academic Journals (OUP)</source><creator>Wang, L. ; Nakamura, H. ; Kage‐Nakadai, E. ; Hara‐Kudo, Y. ; Nishikawa, Y.</creator><creatorcontrib>Wang, L. ; Nakamura, H. ; Kage‐Nakadai, E. ; Hara‐Kudo, Y. ; Nishikawa, Y.</creatorcontrib><description>Aim
This study assessed whether multilocus variable‐number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people.
Methods and Results
MLVA genotyping of 142 aEPEC strains isolated from foods and faecal samples of domestic animals and humans revealed 126 distinct MLVA profiles that distributed to four clusters, yielding a Simpson's index of diversity (D) of 99·8%. Cluster 2 included 87% of cattle isolates and 67% of patient isolates. The plurality (15/34, 44%) of strains from healthy humans mapped to Cluster 1, while half (18/41, 44%) of the swine strains belonged to Cluster 4. Testing for antimicrobial susceptibility revealed that 52 strains (37%) of aEPEC were resistant to one or more agents; only 10 strains (7%) exhibited resistance to more than three agents. Strains isolated from swine or food exhibited a wider variety of resistance phenotypes than bovine or human strains.
Conclusions
MLVA assigned the aEPEC isolates from cattle and patients to Cluster 2, distinct from aEPEC from other sources. Hog yards may be a larger source of drug‐resistant strains than are cattle ranches.
Significance and Impact of the Study
MLVA suggests that human diarrhoeagenic aEPEC are derived from cattle and are distinct from strains carried by healthy people and other animals. Cattle appear to be reservoirs of human diarrhoeagenic aEPEC.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13322</identifier><identifier>PMID: 27718315</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Cattle ; Diarrhea ; Drug resistance ; Drug Resistance, Bacterial ; E coli ; Enteropathogenic Escherichia coli - classification ; Enteropathogenic Escherichia coli - drug effects ; Enteropathogenic Escherichia coli - genetics ; Enteropathogenic Escherichia coli - isolation & purification ; Escherichia coli ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Feces ; Feces - microbiology ; Food Microbiology ; food safety ; Genotype ; genotyping ; Humans ; Minisatellite Repeats ; molecular epidemiology ; Swine</subject><ispartof>Journal of applied microbiology, 2017-01, Vol.122 (1), p.268-278</ispartof><rights>2016 The Authors. published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.</rights><rights>2016 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.</rights><rights>Copyright © 2017 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4302-5733d8a3134dece792893cdd5603bd17e6d3cbf9026a3626708a4461e572c5293</citedby><cites>FETCH-LOGICAL-c4302-5733d8a3134dece792893cdd5603bd17e6d3cbf9026a3626708a4461e572c5293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13322$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13322$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27718315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Nakamura, H.</creatorcontrib><creatorcontrib>Kage‐Nakadai, E.</creatorcontrib><creatorcontrib>Hara‐Kudo, Y.</creatorcontrib><creatorcontrib>Nishikawa, Y.</creatorcontrib><title>Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
This study assessed whether multilocus variable‐number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people.
Methods and Results
MLVA genotyping of 142 aEPEC strains isolated from foods and faecal samples of domestic animals and humans revealed 126 distinct MLVA profiles that distributed to four clusters, yielding a Simpson's index of diversity (D) of 99·8%. Cluster 2 included 87% of cattle isolates and 67% of patient isolates. The plurality (15/34, 44%) of strains from healthy humans mapped to Cluster 1, while half (18/41, 44%) of the swine strains belonged to Cluster 4. Testing for antimicrobial susceptibility revealed that 52 strains (37%) of aEPEC were resistant to one or more agents; only 10 strains (7%) exhibited resistance to more than three agents. Strains isolated from swine or food exhibited a wider variety of resistance phenotypes than bovine or human strains.
Conclusions
MLVA assigned the aEPEC isolates from cattle and patients to Cluster 2, distinct from aEPEC from other sources. Hog yards may be a larger source of drug‐resistant strains than are cattle ranches.
Significance and Impact of the Study
MLVA suggests that human diarrhoeagenic aEPEC are derived from cattle and are distinct from strains carried by healthy people and other animals. Cattle appear to be reservoirs of human diarrhoeagenic aEPEC.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Cattle</subject><subject>Diarrhea</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>E coli</subject><subject>Enteropathogenic Escherichia coli - classification</subject><subject>Enteropathogenic Escherichia coli - drug effects</subject><subject>Enteropathogenic Escherichia coli - genetics</subject><subject>Enteropathogenic Escherichia coli - isolation & purification</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Food Microbiology</subject><subject>food safety</subject><subject>Genotype</subject><subject>genotyping</subject><subject>Humans</subject><subject>Minisatellite Repeats</subject><subject>molecular epidemiology</subject><subject>Swine</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNqNksuKFDEYhQtRnHF04QtIwI0uaiaXSl2WQzPeGHGj6-Kv5K_pNLmUSZXSOx_Bx3Ptk5jual0IgoGQn-TjnHA4RfGU0UuW19UO3CUTgvN7xTkTtSx53fD7x7kqJW34WfEopR2lTFBZPyzOeNOwVjB5XvzYBDdBNCl4MuyJW-xsbFBLIl_yLQwWf3777hc3YCQzeI2ORJwQZgIe7D6ZlAed92ycUTEMBmwm8n2mFRJwwd8RmPeTUfkF_YwxTDBvwx16o8hNUluMRm0NEBWsIWmOYHwi-UcWZtRkjMGRMQRNErjJ4mq4XRz4k7VxWXkEPBikCZVx6NPj4sEINuGT03lRfHp183Hzprz98Prt5vq2VJWgvJSNELoFwUSlUWHT8bYTSmtZUzFo1mCthRrGjvIaRJ1zpS1UVc1QNlxJ3omL4sWqO8XwecE0984khdaCx7CknrWyq9qmpe1_oEKKtu6EyOjzv9BdWGJO_CjIGyqZPHi_XKkcfEoRx36KOYy47xntD83oczP6YzMy--ykuAwO9R_ydxUycLUCX43F_b-V-nfX71fJXzxGyAw</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Wang, L.</creator><creator>Nakamura, H.</creator><creator>Kage‐Nakadai, E.</creator><creator>Hara‐Kudo, Y.</creator><creator>Nishikawa, Y.</creator><general>Oxford University Press</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201701</creationdate><title>Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens</title><author>Wang, L. ; Nakamura, H. ; Kage‐Nakadai, E. ; Hara‐Kudo, Y. ; Nishikawa, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4302-5733d8a3134dece792893cdd5603bd17e6d3cbf9026a3626708a4461e572c5293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Cattle</topic><topic>Diarrhea</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>E coli</topic><topic>Enteropathogenic Escherichia coli - classification</topic><topic>Enteropathogenic Escherichia coli - drug effects</topic><topic>Enteropathogenic Escherichia coli - genetics</topic><topic>Enteropathogenic Escherichia coli - isolation & purification</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Food Microbiology</topic><topic>food safety</topic><topic>Genotype</topic><topic>genotyping</topic><topic>Humans</topic><topic>Minisatellite Repeats</topic><topic>molecular epidemiology</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Nakamura, H.</creatorcontrib><creatorcontrib>Kage‐Nakadai, E.</creatorcontrib><creatorcontrib>Hara‐Kudo, Y.</creatorcontrib><creatorcontrib>Nishikawa, Y.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, L.</au><au>Nakamura, H.</au><au>Kage‐Nakadai, E.</au><au>Hara‐Kudo, Y.</au><au>Nishikawa, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2017-01</date><risdate>2017</risdate><volume>122</volume><issue>1</issue><spage>268</spage><epage>278</epage><pages>268-278</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aim
This study assessed whether multilocus variable‐number tandem repeat analysis (MLVA) and antimicrobial susceptibility testing discriminated diarrhoeagenic atypical enteropathogenic Escherichia coli (aEPEC) from aEPEC indigenous to domestic animals or healthy people.
Methods and Results
MLVA genotyping of 142 aEPEC strains isolated from foods and faecal samples of domestic animals and humans revealed 126 distinct MLVA profiles that distributed to four clusters, yielding a Simpson's index of diversity (D) of 99·8%. Cluster 2 included 87% of cattle isolates and 67% of patient isolates. The plurality (15/34, 44%) of strains from healthy humans mapped to Cluster 1, while half (18/41, 44%) of the swine strains belonged to Cluster 4. Testing for antimicrobial susceptibility revealed that 52 strains (37%) of aEPEC were resistant to one or more agents; only 10 strains (7%) exhibited resistance to more than three agents. Strains isolated from swine or food exhibited a wider variety of resistance phenotypes than bovine or human strains.
Conclusions
MLVA assigned the aEPEC isolates from cattle and patients to Cluster 2, distinct from aEPEC from other sources. Hog yards may be a larger source of drug‐resistant strains than are cattle ranches.
Significance and Impact of the Study
MLVA suggests that human diarrhoeagenic aEPEC are derived from cattle and are distinct from strains carried by healthy people and other animals. Cattle appear to be reservoirs of human diarrhoeagenic aEPEC.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27718315</pmid><doi>10.1111/jam.13322</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - pharmacology Antibiotics Cattle Diarrhea Drug resistance Drug Resistance, Bacterial E coli Enteropathogenic Escherichia coli - classification Enteropathogenic Escherichia coli - drug effects Enteropathogenic Escherichia coli - genetics Enteropathogenic Escherichia coli - isolation & purification Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Feces Feces - microbiology Food Microbiology food safety Genotype genotyping Humans Minisatellite Repeats molecular epidemiology Swine |
| title | Comparison by multilocus variable‐number tandem repeat analysis and antimicrobial resistance among atypical enteropathogenic Escherichia coli strains isolated from food samples and human and animal faecal specimens |
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