Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy
Aims To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy. Methods and Results Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes wer...
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| Veröffentlicht in: | Journal of applied microbiology 2017-01, Vol.122 (1), p.239-247 |
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| creator | Tozzoli, R. Di Bartolo, I. Gigliucci, F. Brambilla, G. Monini, M. Vignolo, E. Caprioli, A. Morabito, S. |
| description | Aims
To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy.
Methods and Results
Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes were analysed by real time PCR for the presence of hepatitis E virus (HEV), porcine and human adenovirus (HuAdV), norovirus, rotavirus and diarrhoeagenic E. coli. None of the samples was found positive for HEV or rotavirus. Four samples were positive for the presence of nucleic acids from human norovirus, two of them being also positive for HuAdV. Real time PCR screening gave positive results for many of the virulence genes characteristic of diarrhoeagenic E. coli in 21 samples. These included the verocytotoxin‐coding genes, in some cases associated with intimin‐coding gene, and markers of enteroaggregative, enterotoxigenic and enteroinvasive E. coli.
Conclusions
These results provide evidence that enteric viruses and pathogenic E. coli may be released into the environment through the use of sludge‐derived TSI.
Significance and Impact of the Study
The results highlight that the TSI‐related environmental risk for the food chain should be more deeply assessed. |
| doi_str_mv | 10.1111/jam.13308 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859486304</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1859486304</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3868-7bb5df675c7993718f42b3d3c5a586830ecc15244b4697f62007a5022d7076243</originalsourceid><addsrcrecordid>eNqNkctKxDAUhoMozji68AUk4EYXnck97XIYvKLoQtclTVMnQ9uMSavM25u56EIQPJsccj4-zuEH4BSjMY41WahmjClF6R4YYip4QoQk-5ueJRxJMgBHISwQwhRxcQgGRIqUpRkdgvJZdXP3Zlqr4VXQc-OtnlsFtastVG0JTdut_-CH9X0wAdoWFtaFOC7DBvCmVp0pYeeWMDhbQ9ssvfswfsPedapeHYODStXBnOzeEXi9vnqZ3SYPTzd3s-lDomkq0kQWBS8rIbmWWUYlTitGClpSzRWPc4qM1pgTxgomMlkJgpBUHBFSSiQFYXQELrbeuMB7b0KXNzZoU9eqNa4POU55xlJB0X9QhmXGOSYRPf-FLlzv23jIWkgk4jguNwKXW0p7F4I3Vb70tlF-lWOUr1PKY0r5JqXInu2MfdGY8of8jiUCky3waWuz-tuU308ft8ovXm-Zgw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1852705168</pqid></control><display><type>article</type><title>Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Tozzoli, R. ; Di Bartolo, I. ; Gigliucci, F. ; Brambilla, G. ; Monini, M. ; Vignolo, E. ; Caprioli, A. ; Morabito, S.</creator><creatorcontrib>Tozzoli, R. ; Di Bartolo, I. ; Gigliucci, F. ; Brambilla, G. ; Monini, M. ; Vignolo, E. ; Caprioli, A. ; Morabito, S.</creatorcontrib><description>Aims
To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy.
Methods and Results
Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes were analysed by real time PCR for the presence of hepatitis E virus (HEV), porcine and human adenovirus (HuAdV), norovirus, rotavirus and diarrhoeagenic E. coli. None of the samples was found positive for HEV or rotavirus. Four samples were positive for the presence of nucleic acids from human norovirus, two of them being also positive for HuAdV. Real time PCR screening gave positive results for many of the virulence genes characteristic of diarrhoeagenic E. coli in 21 samples. These included the verocytotoxin‐coding genes, in some cases associated with intimin‐coding gene, and markers of enteroaggregative, enterotoxigenic and enteroinvasive E. coli.
Conclusions
These results provide evidence that enteric viruses and pathogenic E. coli may be released into the environment through the use of sludge‐derived TSI.
Significance and Impact of the Study
The results highlight that the TSI‐related environmental risk for the food chain should be more deeply assessed.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13308</identifier><identifier>PMID: 27684893</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenoviridae ; Animals ; E coli ; Enterovirus - classification ; Enterovirus - genetics ; Enterovirus - isolation & purification ; environmental ; Escherichia coli ; Escherichia coli (all potentially pathogenic types) ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Hepatitis E virus ; Hepeviridae ; Humans ; Italy ; Manure - microbiology ; Manure - virology ; microbial contamination ; Microbiology ; Norovirus ; Reoviridae ; Sewage - microbiology ; Sewage - virology ; Sludge ; sludges ; Soil - chemistry ; Soil Microbiology ; Soils ; Swine ; Viruses</subject><ispartof>Journal of applied microbiology, 2017-01, Vol.122 (1), p.239-247</ispartof><rights>2016 The Society for Applied Microbiology</rights><rights>2016 The Society for Applied Microbiology.</rights><rights>Copyright © 2017 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3868-7bb5df675c7993718f42b3d3c5a586830ecc15244b4697f62007a5022d7076243</citedby><cites>FETCH-LOGICAL-c3868-7bb5df675c7993718f42b3d3c5a586830ecc15244b4697f62007a5022d7076243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13308$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13308$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27915,27916,45565,45566</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27684893$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tozzoli, R.</creatorcontrib><creatorcontrib>Di Bartolo, I.</creatorcontrib><creatorcontrib>Gigliucci, F.</creatorcontrib><creatorcontrib>Brambilla, G.</creatorcontrib><creatorcontrib>Monini, M.</creatorcontrib><creatorcontrib>Vignolo, E.</creatorcontrib><creatorcontrib>Caprioli, A.</creatorcontrib><creatorcontrib>Morabito, S.</creatorcontrib><title>Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy.
Methods and Results
Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes were analysed by real time PCR for the presence of hepatitis E virus (HEV), porcine and human adenovirus (HuAdV), norovirus, rotavirus and diarrhoeagenic E. coli. None of the samples was found positive for HEV or rotavirus. Four samples were positive for the presence of nucleic acids from human norovirus, two of them being also positive for HuAdV. Real time PCR screening gave positive results for many of the virulence genes characteristic of diarrhoeagenic E. coli in 21 samples. These included the verocytotoxin‐coding genes, in some cases associated with intimin‐coding gene, and markers of enteroaggregative, enterotoxigenic and enteroinvasive E. coli.
Conclusions
These results provide evidence that enteric viruses and pathogenic E. coli may be released into the environment through the use of sludge‐derived TSI.
Significance and Impact of the Study
The results highlight that the TSI‐related environmental risk for the food chain should be more deeply assessed.</description><subject>Adenoviridae</subject><subject>Animals</subject><subject>E coli</subject><subject>Enterovirus - classification</subject><subject>Enterovirus - genetics</subject><subject>Enterovirus - isolation & purification</subject><subject>environmental</subject><subject>Escherichia coli</subject><subject>Escherichia coli (all potentially pathogenic types)</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Hepatitis E virus</subject><subject>Hepeviridae</subject><subject>Humans</subject><subject>Italy</subject><subject>Manure - microbiology</subject><subject>Manure - virology</subject><subject>microbial contamination</subject><subject>Microbiology</subject><subject>Norovirus</subject><subject>Reoviridae</subject><subject>Sewage - microbiology</subject><subject>Sewage - virology</subject><subject>Sludge</subject><subject>sludges</subject><subject>Soil - chemistry</subject><subject>Soil Microbiology</subject><subject>Soils</subject><subject>Swine</subject><subject>Viruses</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctKxDAUhoMozji68AUk4EYXnck97XIYvKLoQtclTVMnQ9uMSavM25u56EIQPJsccj4-zuEH4BSjMY41WahmjClF6R4YYip4QoQk-5ueJRxJMgBHISwQwhRxcQgGRIqUpRkdgvJZdXP3Zlqr4VXQc-OtnlsFtastVG0JTdut_-CH9X0wAdoWFtaFOC7DBvCmVp0pYeeWMDhbQ9ssvfswfsPedapeHYODStXBnOzeEXi9vnqZ3SYPTzd3s-lDomkq0kQWBS8rIbmWWUYlTitGClpSzRWPc4qM1pgTxgomMlkJgpBUHBFSSiQFYXQELrbeuMB7b0KXNzZoU9eqNa4POU55xlJB0X9QhmXGOSYRPf-FLlzv23jIWkgk4jguNwKXW0p7F4I3Vb70tlF-lWOUr1PKY0r5JqXInu2MfdGY8of8jiUCky3waWuz-tuU308ft8ovXm-Zgw</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Tozzoli, R.</creator><creator>Di Bartolo, I.</creator><creator>Gigliucci, F.</creator><creator>Brambilla, G.</creator><creator>Monini, M.</creator><creator>Vignolo, E.</creator><creator>Caprioli, A.</creator><creator>Morabito, S.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201701</creationdate><title>Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy</title><author>Tozzoli, R. ; Di Bartolo, I. ; Gigliucci, F. ; Brambilla, G. ; Monini, M. ; Vignolo, E. ; Caprioli, A. ; Morabito, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3868-7bb5df675c7993718f42b3d3c5a586830ecc15244b4697f62007a5022d7076243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adenoviridae</topic><topic>Animals</topic><topic>E coli</topic><topic>Enterovirus - classification</topic><topic>Enterovirus - genetics</topic><topic>Enterovirus - isolation & purification</topic><topic>environmental</topic><topic>Escherichia coli</topic><topic>Escherichia coli (all potentially pathogenic types)</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Hepatitis E virus</topic><topic>Hepeviridae</topic><topic>Humans</topic><topic>Italy</topic><topic>Manure - microbiology</topic><topic>Manure - virology</topic><topic>microbial contamination</topic><topic>Microbiology</topic><topic>Norovirus</topic><topic>Reoviridae</topic><topic>Sewage - microbiology</topic><topic>Sewage - virology</topic><topic>Sludge</topic><topic>sludges</topic><topic>Soil - chemistry</topic><topic>Soil Microbiology</topic><topic>Soils</topic><topic>Swine</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tozzoli, R.</creatorcontrib><creatorcontrib>Di Bartolo, I.</creatorcontrib><creatorcontrib>Gigliucci, F.</creatorcontrib><creatorcontrib>Brambilla, G.</creatorcontrib><creatorcontrib>Monini, M.</creatorcontrib><creatorcontrib>Vignolo, E.</creatorcontrib><creatorcontrib>Caprioli, A.</creatorcontrib><creatorcontrib>Morabito, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tozzoli, R.</au><au>Di Bartolo, I.</au><au>Gigliucci, F.</au><au>Brambilla, G.</au><au>Monini, M.</au><au>Vignolo, E.</au><au>Caprioli, A.</au><au>Morabito, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2017-01</date><risdate>2017</risdate><volume>122</volume><issue>1</issue><spage>239</spage><epage>247</epage><pages>239-247</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To investigate the presence of genomic traits associated with a set of enteric viruses as well as pathogenic Escherichia coli in top soil improvers (TSI) from Italy.
Methods and Results
Twenty‐four TSI samples originating from municipal sewage sludges, pig manure, green and household wastes were analysed by real time PCR for the presence of hepatitis E virus (HEV), porcine and human adenovirus (HuAdV), norovirus, rotavirus and diarrhoeagenic E. coli. None of the samples was found positive for HEV or rotavirus. Four samples were positive for the presence of nucleic acids from human norovirus, two of them being also positive for HuAdV. Real time PCR screening gave positive results for many of the virulence genes characteristic of diarrhoeagenic E. coli in 21 samples. These included the verocytotoxin‐coding genes, in some cases associated with intimin‐coding gene, and markers of enteroaggregative, enterotoxigenic and enteroinvasive E. coli.
Conclusions
These results provide evidence that enteric viruses and pathogenic E. coli may be released into the environment through the use of sludge‐derived TSI.
Significance and Impact of the Study
The results highlight that the TSI‐related environmental risk for the food chain should be more deeply assessed.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27684893</pmid><doi>10.1111/jam.13308</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of applied microbiology, 2017-01, Vol.122 (1), p.239-247 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adenoviridae Animals E coli Enterovirus - classification Enterovirus - genetics Enterovirus - isolation & purification environmental Escherichia coli Escherichia coli (all potentially pathogenic types) Escherichia coli - genetics Escherichia coli - isolation & purification Hepatitis E virus Hepeviridae Humans Italy Manure - microbiology Manure - virology microbial contamination Microbiology Norovirus Reoviridae Sewage - microbiology Sewage - virology Sludge sludges Soil - chemistry Soil Microbiology Soils Swine Viruses |
| title | Pathogenic Escherichia coli and enteric viruses in biosolids and related top soil improvers in Italy |
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