Biological effects of TiO2 and CeO2 nanoparticles on the growth, photosynthetic activity, and cellular components of a marine diatom Phaeodactylum tricornutum
It is very important to have a good understanding of the biological effects of nanoparticles (NPs) on marine diatoms. In this study, the physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum to titanium dioxide NPs (nano-TiO2) and cerium oxide NPs (nano-CeO2) were comp...
Gespeichert in:
Veröffentlicht in: | The Science of the total environment 2017-01, Vol.575, p.87-96 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | It is very important to have a good understanding of the biological effects of nanoparticles (NPs) on marine diatoms. In this study, the physiological and biochemical responses of a marine diatom Phaeodactylum tricornutum to titanium dioxide NPs (nano-TiO2) and cerium oxide NPs (nano-CeO2) were compared and evaluated using 96h growth tests in a batch-culture system. At 96h of exposure, the growth inhibition rate (IR, %) of P. tricornutum increased from 5.46 to 27.31% with increasing nano-TiO2 concentrations from 2.5 to 40mgL−1. The maximum IR of 49.59% occurred in 40mgL−1 nano-TiO2 treatments at 48h of exposure. Growth of the diatom was increased in low nano-CeO2 treatments (≤5mgL−1), but was inhibited in high nano-CeO2 treatments (≥10mgL−1). Large aggregates of NPs were attached to the cells of P. tricornutum in 20 and 40mgL−1 nano-TiO2 and nano-CeO2 treatments. In addition, the effective quantum yields (ΦPSII) of P. tricornutum in 40mgL−1 nano-TiO2 and nano-CeO2 treatments were 83.33 and 71.13% of that in the controls at 96h of exposure, respectively. Compared with that of the controls at 96h of exposure, chlorophyll a content, soluble sugar content, malondialdehyde (MDA) content, SOD and POD activities of P. tricornutum in 40mgL−1 nano-TiO2 and nano-CeO2 treatments increased by 57.56, 142.97, 373.25, 698.76, 204.85% and 21.43, 89.41, 194.97, 340.05, 502.86%, while soluble protein content decreased by 70.38 and 28.64%, respectively. These findings will be helpful to understand the effect mechanisms of NPs on marine organisms.
[Display omitted]
•Photosynthetic activity of P. tricornutum was inhibited by nano-TiO2 and nano-CeO2.•Soluble sugar may serve as an osmotic agent to improve resistance of the diatom.•Physiological effects of both NPs on the diatom were caused by ROS generation.•SOD and POD were considered as a stimulatory response of the diatom to both NPs. |
---|---|
ISSN: | 0048-9697 1879-1026 |
DOI: | 10.1016/j.scitotenv.2016.10.003 |