Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus
•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge. Porcine reproductive and respiratory syndrome virus (PRRSV) has been causi...
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creator | Du, Luping Li, Bin Pang, Fengjiao Yu, Zhengyu Xu, Xiangwei Fan, Baochao Tan, Yeping He, Kongwang Huang, Kehe |
description | •A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination. |
doi_str_mv | 10.1016/j.vetimm.2016.12.001 |
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Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2016.12.001</identifier><identifier>PMID: 28063474</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject><![CDATA[Animals ; Body Temperature ; Cercopithecus aethiops ; DNA vaccine ; DNA, Viral ; Glutathione Peroxidase - genetics ; Glutathione Peroxidase - immunology ; GPX1 ; Immune response ; Immunity, Cellular ; Immunity, Humoral ; Immunogenicity, Vaccine ; Mice ; Mice, Inbred BALB C ; Plasmids ; Porcine Reproductive and Respiratory Syndrome - immunology ; Porcine Reproductive and Respiratory Syndrome - pathology ; Porcine Reproductive and Respiratory Syndrome - prevention & control ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - immunology ; PRRSV ; Swine ; Swine Diseases - immunology ; Swine Diseases - pathology ; Swine Diseases - prevention & control ; Swine Diseases - virology ; T-Lymphocytes - immunology ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology ; Vero Cells ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Viremia - prevention & control]]></subject><ispartof>Veterinary immunology and immunopathology, 2017-01, Vol.183, p.31-39</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</citedby><cites>FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242716303403$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28063474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Luping</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Pang, Fengjiao</creatorcontrib><creatorcontrib>Yu, Zhengyu</creatorcontrib><creatorcontrib>Xu, Xiangwei</creatorcontrib><creatorcontrib>Fan, Baochao</creatorcontrib><creatorcontrib>Tan, Yeping</creatorcontrib><creatorcontrib>He, Kongwang</creatorcontrib><creatorcontrib>Huang, Kehe</creatorcontrib><title>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.</description><subject>Animals</subject><subject>Body Temperature</subject><subject>Cercopithecus aethiops</subject><subject>DNA vaccine</subject><subject>DNA, Viral</subject><subject>Glutathione Peroxidase - genetics</subject><subject>Glutathione Peroxidase - immunology</subject><subject>GPX1</subject><subject>Immune response</subject><subject>Immunity, Cellular</subject><subject>Immunity, Humoral</subject><subject>Immunogenicity, Vaccine</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Plasmids</subject><subject>Porcine Reproductive and Respiratory Syndrome - immunology</subject><subject>Porcine Reproductive and Respiratory Syndrome - pathology</subject><subject>Porcine Reproductive and Respiratory Syndrome - prevention & control</subject><subject>Porcine respiratory and reproductive syndrome virus</subject><subject>Porcine respiratory and reproductive syndrome virus - genetics</subject><subject>Porcine respiratory and reproductive syndrome virus - immunology</subject><subject>PRRSV</subject><subject>Swine</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - prevention & control</subject><subject>Swine Diseases - virology</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Vero Cells</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Viremia - prevention & control</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r3DAQhkVpSDYf_6AUHXuxo09LuhRC0mwCocmhhdyELM0m2q7trWQb9t9XYbc9hp7EaJ5XM-hB6BMlNSW0uVzXM4yx62pWqpqymhD6AS2oVrxikouPaFEasmKCqRN0mvOaECKN1sfohGnScKHEAv16GpKPPeDl0zPF0L-63kMulaxalyHgm-9XeHa-MG6MQ4_di4t9HvH2kEuwTUOY_BhnwK4P5SJvY3LjkHY47_qQhg7wHNOUz9HRym0yXBzOM_Tz9tuP67vq4XF5f331UHkh6FgZ0QTOlG6VUytiuBDAJeWtZ0JTwxsqmFxRrwgYxVsTlAPDgjFMtISKIPkZ-rJ_t2z2e4I82i5mD5uN62GYsqVaGqGZ1s3_oI1ulJa6oGKP-jTknGBltyl2Lu0sJfbNiF3bvRH7ZsRSZouREvt8mDC1HYR_ob8KCvB1D0D5kjlCstlHKBZCTOBHG4b4_oQ_NRedwg</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Du, Luping</creator><creator>Li, Bin</creator><creator>Pang, Fengjiao</creator><creator>Yu, Zhengyu</creator><creator>Xu, Xiangwei</creator><creator>Fan, Baochao</creator><creator>Tan, Yeping</creator><creator>He, Kongwang</creator><creator>Huang, Kehe</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201701</creationdate><title>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</title><author>Du, Luping ; Li, Bin ; Pang, Fengjiao ; Yu, Zhengyu ; Xu, Xiangwei ; Fan, Baochao ; Tan, Yeping ; He, Kongwang ; Huang, Kehe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Body Temperature</topic><topic>Cercopithecus aethiops</topic><topic>DNA vaccine</topic><topic>DNA, Viral</topic><topic>Glutathione Peroxidase - genetics</topic><topic>Glutathione Peroxidase - immunology</topic><topic>GPX1</topic><topic>Immune response</topic><topic>Immunity, Cellular</topic><topic>Immunity, Humoral</topic><topic>Immunogenicity, Vaccine</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Plasmids</topic><topic>Porcine Reproductive and Respiratory Syndrome - immunology</topic><topic>Porcine Reproductive and Respiratory Syndrome - pathology</topic><topic>Porcine Reproductive and Respiratory Syndrome - prevention & control</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - immunology</topic><topic>PRRSV</topic><topic>Swine</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - prevention & control</topic><topic>Swine Diseases - virology</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Vero Cells</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Viremia - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Luping</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Pang, Fengjiao</creatorcontrib><creatorcontrib>Yu, Zhengyu</creatorcontrib><creatorcontrib>Xu, Xiangwei</creatorcontrib><creatorcontrib>Fan, Baochao</creatorcontrib><creatorcontrib>Tan, Yeping</creatorcontrib><creatorcontrib>He, Kongwang</creatorcontrib><creatorcontrib>Huang, Kehe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Luping</au><au>Li, Bin</au><au>Pang, Fengjiao</au><au>Yu, Zhengyu</au><au>Xu, Xiangwei</au><au>Fan, Baochao</au><au>Tan, Yeping</au><au>He, Kongwang</au><au>Huang, Kehe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2017-01</date><risdate>2017</risdate><volume>183</volume><spage>31</spage><epage>39</epage><pages>31-39</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28063474</pmid><doi>10.1016/j.vetimm.2016.12.001</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Body Temperature Cercopithecus aethiops DNA vaccine DNA, Viral Glutathione Peroxidase - genetics Glutathione Peroxidase - immunology GPX1 Immune response Immunity, Cellular Immunity, Humoral Immunogenicity, Vaccine Mice Mice, Inbred BALB C Plasmids Porcine Reproductive and Respiratory Syndrome - immunology Porcine Reproductive and Respiratory Syndrome - pathology Porcine Reproductive and Respiratory Syndrome - prevention & control Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - immunology PRRSV Swine Swine Diseases - immunology Swine Diseases - pathology Swine Diseases - prevention & control Swine Diseases - virology T-Lymphocytes - immunology Vaccines, DNA - administration & dosage Vaccines, DNA - immunology Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - immunology Vero Cells Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology Viremia - prevention & control |
title | Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus |
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