Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus

•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge. Porcine reproductive and respiratory syndrome virus (PRRSV) has been causi...

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Veröffentlicht in:Veterinary immunology and immunopathology 2017-01, Vol.183, p.31-39
Hauptverfasser: Du, Luping, Li, Bin, Pang, Fengjiao, Yu, Zhengyu, Xu, Xiangwei, Fan, Baochao, Tan, Yeping, He, Kongwang, Huang, Kehe
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container_title Veterinary immunology and immunopathology
container_volume 183
creator Du, Luping
Li, Bin
Pang, Fengjiao
Yu, Zhengyu
Xu, Xiangwei
Fan, Baochao
Tan, Yeping
He, Kongwang
Huang, Kehe
description •A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge. Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.
doi_str_mv 10.1016/j.vetimm.2016.12.001
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Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2016.12.001</identifier><identifier>PMID: 28063474</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject><![CDATA[Animals ; Body Temperature ; Cercopithecus aethiops ; DNA vaccine ; DNA, Viral ; Glutathione Peroxidase - genetics ; Glutathione Peroxidase - immunology ; GPX1 ; Immune response ; Immunity, Cellular ; Immunity, Humoral ; Immunogenicity, Vaccine ; Mice ; Mice, Inbred BALB C ; Plasmids ; Porcine Reproductive and Respiratory Syndrome - immunology ; Porcine Reproductive and Respiratory Syndrome - pathology ; Porcine Reproductive and Respiratory Syndrome - prevention & control ; Porcine respiratory and reproductive syndrome virus ; Porcine respiratory and reproductive syndrome virus - genetics ; Porcine respiratory and reproductive syndrome virus - immunology ; PRRSV ; Swine ; Swine Diseases - immunology ; Swine Diseases - pathology ; Swine Diseases - prevention & control ; Swine Diseases - virology ; T-Lymphocytes - immunology ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - immunology ; Vero Cells ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Viremia - prevention & control]]></subject><ispartof>Veterinary immunology and immunopathology, 2017-01, Vol.183, p.31-39</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</citedby><cites>FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242716303403$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28063474$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Luping</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Pang, Fengjiao</creatorcontrib><creatorcontrib>Yu, Zhengyu</creatorcontrib><creatorcontrib>Xu, Xiangwei</creatorcontrib><creatorcontrib>Fan, Baochao</creatorcontrib><creatorcontrib>Tan, Yeping</creatorcontrib><creatorcontrib>He, Kongwang</creatorcontrib><creatorcontrib>Huang, Kehe</creatorcontrib><title>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge. Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. 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control</subject><subject>Swine Diseases - virology</subject><subject>T-Lymphocytes - immunology</subject><subject>Vaccines, DNA - administration &amp; dosage</subject><subject>Vaccines, DNA - immunology</subject><subject>Vaccines, Synthetic - administration &amp; dosage</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Vero Cells</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Vaccines - administration &amp; dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Viremia - prevention &amp; control</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r3DAQhkVpSDYf_6AUHXuxo09LuhRC0mwCocmhhdyELM0m2q7trWQb9t9XYbc9hp7EaJ5XM-hB6BMlNSW0uVzXM4yx62pWqpqymhD6AS2oVrxikouPaFEasmKCqRN0mvOaECKN1sfohGnScKHEAv16GpKPPeDl0zPF0L-63kMulaxalyHgm-9XeHa-MG6MQ4_di4t9HvH2kEuwTUOY_BhnwK4P5SJvY3LjkHY47_qQhg7wHNOUz9HRym0yXBzOM_Tz9tuP67vq4XF5f331UHkh6FgZ0QTOlG6VUytiuBDAJeWtZ0JTwxsqmFxRrwgYxVsTlAPDgjFMtISKIPkZ-rJ_t2z2e4I82i5mD5uN62GYsqVaGqGZ1s3_oI1ulJa6oGKP-jTknGBltyl2Lu0sJfbNiF3bvRH7ZsRSZouREvt8mDC1HYR_ob8KCvB1D0D5kjlCstlHKBZCTOBHG4b4_oQ_NRedwg</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Du, Luping</creator><creator>Li, Bin</creator><creator>Pang, Fengjiao</creator><creator>Yu, Zhengyu</creator><creator>Xu, Xiangwei</creator><creator>Fan, Baochao</creator><creator>Tan, Yeping</creator><creator>He, Kongwang</creator><creator>Huang, Kehe</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201701</creationdate><title>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</title><author>Du, Luping ; Li, Bin ; Pang, Fengjiao ; Yu, Zhengyu ; Xu, Xiangwei ; Fan, Baochao ; Tan, Yeping ; He, Kongwang ; Huang, Kehe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-946d3278b7a7f09344e3513bc24819361425f1c70e973b9d7ae92d9924b014d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Body Temperature</topic><topic>Cercopithecus aethiops</topic><topic>DNA vaccine</topic><topic>DNA, Viral</topic><topic>Glutathione Peroxidase - genetics</topic><topic>Glutathione Peroxidase - immunology</topic><topic>GPX1</topic><topic>Immune response</topic><topic>Immunity, Cellular</topic><topic>Immunity, Humoral</topic><topic>Immunogenicity, Vaccine</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Plasmids</topic><topic>Porcine Reproductive and Respiratory Syndrome - immunology</topic><topic>Porcine Reproductive and Respiratory Syndrome - pathology</topic><topic>Porcine Reproductive and Respiratory Syndrome - prevention &amp; control</topic><topic>Porcine respiratory and reproductive syndrome virus</topic><topic>Porcine respiratory and reproductive syndrome virus - genetics</topic><topic>Porcine respiratory and reproductive syndrome virus - immunology</topic><topic>PRRSV</topic><topic>Swine</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - prevention &amp; control</topic><topic>Swine Diseases - virology</topic><topic>T-Lymphocytes - immunology</topic><topic>Vaccines, DNA - administration &amp; dosage</topic><topic>Vaccines, DNA - immunology</topic><topic>Vaccines, Synthetic - administration &amp; dosage</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Vero Cells</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Vaccines - administration &amp; dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Viremia - prevention &amp; control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Luping</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Pang, Fengjiao</creatorcontrib><creatorcontrib>Yu, Zhengyu</creatorcontrib><creatorcontrib>Xu, Xiangwei</creatorcontrib><creatorcontrib>Fan, Baochao</creatorcontrib><creatorcontrib>Tan, Yeping</creatorcontrib><creatorcontrib>He, Kongwang</creatorcontrib><creatorcontrib>Huang, Kehe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Luping</au><au>Li, Bin</au><au>Pang, Fengjiao</au><au>Yu, Zhengyu</au><au>Xu, Xiangwei</au><au>Fan, Baochao</au><au>Tan, Yeping</au><au>He, Kongwang</au><au>Huang, Kehe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2017-01</date><risdate>2017</risdate><volume>183</volume><spage>31</spage><epage>39</epage><pages>31-39</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>•A PRRSV DNA vaccine candidate(pcDNA3.1-GPX1-LSynORF5) is constructed.•GPX1 improves immune responses induced by pcDNA3.1-SynORF5 in mice and pigs.•The prepared DNA vaccine provides partial protection against PRRSV challenge. Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28063474</pmid><doi>10.1016/j.vetimm.2016.12.001</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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ispartof Veterinary immunology and immunopathology, 2017-01, Vol.183, p.31-39
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Body Temperature
Cercopithecus aethiops
DNA vaccine
DNA, Viral
Glutathione Peroxidase - genetics
Glutathione Peroxidase - immunology
GPX1
Immune response
Immunity, Cellular
Immunity, Humoral
Immunogenicity, Vaccine
Mice
Mice, Inbred BALB C
Plasmids
Porcine Reproductive and Respiratory Syndrome - immunology
Porcine Reproductive and Respiratory Syndrome - pathology
Porcine Reproductive and Respiratory Syndrome - prevention & control
Porcine respiratory and reproductive syndrome virus
Porcine respiratory and reproductive syndrome virus - genetics
Porcine respiratory and reproductive syndrome virus - immunology
PRRSV
Swine
Swine Diseases - immunology
Swine Diseases - pathology
Swine Diseases - prevention & control
Swine Diseases - virology
T-Lymphocytes - immunology
Vaccines, DNA - administration & dosage
Vaccines, DNA - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vero Cells
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Viremia - prevention & control
title Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus
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