Genetic polymorphism of ten MiniSTR loci in the population of Punjab Pakistan for forensic application
Ten MiniSTR loci were analyzed on 250 unrelated individuals of Punjabi population from Punjab province of Pakistan. The product amplification range is from 65 to 280 bp. Allele frequency for a total of 98 observed alleles is from 0.002 to 0.41. Forensic and paternity statistical parameters were inve...
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| Veröffentlicht in: | International journal of legal medicine 2015-09, Vol.129 (5), p.937-942 |
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| creator | Shafique, Muhammad Shahzad, Muhammad Saqib Shan, Muhammad Adnan Ali, Azam Rahman, Ziaur Husnain, Tayyab |
| description | Ten MiniSTR loci were analyzed on 250 unrelated individuals of Punjabi population from Punjab province of Pakistan. The product amplification range is from 65 to 280 bp. Allele frequency for a total of 98 observed alleles is from 0.002 to 0.41. Forensic and paternity statistical parameters were investigated including combined power of discrimination (PD), combined power of exclusion (PE), and cumulative probability of matching (PM) equaled to 0.99999999999824, 0.999824, and 1.75931 × 10
−12
, respectively. These MiniSTRs on the basis of high degree polymorphism and fragment size reduction will be highly informative in most of the forensic cases where DNA of the samples is degraded, mass disasters, and dead body identification. Significant differences were observed in all loci when comparing with same STR loci in previously published different world populations. |
| doi_str_mv | 10.1007/s00414-014-1127-5 |
| format | Article |
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−12
, respectively. These MiniSTRs on the basis of high degree polymorphism and fragment size reduction will be highly informative in most of the forensic cases where DNA of the samples is degraded, mass disasters, and dead body identification. Significant differences were observed in all loci when comparing with same STR loci in previously published different world populations.</description><identifier>ISSN: 0937-9827</identifier><identifier>EISSN: 1437-1596</identifier><identifier>DOI: 10.1007/s00414-014-1127-5</identifier><identifier>PMID: 25422056</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>DNA Fingerprinting ; Forensic Medicine ; Gene Frequency ; Genetic testing ; Genetics, Population ; Humans ; Medical Law ; Medicine ; Medicine & Public Health ; Microsatellite Repeats ; Molecular biology ; Molecular weight ; Original Article ; Pakistan ; Paternity ; Polymorphism ; Polymorphism, Genetic ; Real-Time Polymerase Chain Reaction ; Thermal cycling</subject><ispartof>International journal of legal medicine, 2015-09, Vol.129 (5), p.937-942</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-bcc5db208ba77b1cbc1967255f3d7dd3c9f7dc7b3ed0f949d6dd1d25a25d10d13</citedby><cites>FETCH-LOGICAL-c475t-bcc5db208ba77b1cbc1967255f3d7dd3c9f7dc7b3ed0f949d6dd1d25a25d10d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00414-014-1127-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00414-014-1127-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25422056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shafique, Muhammad</creatorcontrib><creatorcontrib>Shahzad, Muhammad Saqib</creatorcontrib><creatorcontrib>Shan, Muhammad Adnan</creatorcontrib><creatorcontrib>Ali, Azam</creatorcontrib><creatorcontrib>Rahman, Ziaur</creatorcontrib><creatorcontrib>Husnain, Tayyab</creatorcontrib><title>Genetic polymorphism of ten MiniSTR loci in the population of Punjab Pakistan for forensic application</title><title>International journal of legal medicine</title><addtitle>Int J Legal Med</addtitle><addtitle>Int J Legal Med</addtitle><description>Ten MiniSTR loci were analyzed on 250 unrelated individuals of Punjabi population from Punjab province of Pakistan. The product amplification range is from 65 to 280 bp. Allele frequency for a total of 98 observed alleles is from 0.002 to 0.41. Forensic and paternity statistical parameters were investigated including combined power of discrimination (PD), combined power of exclusion (PE), and cumulative probability of matching (PM) equaled to 0.99999999999824, 0.999824, and 1.75931 × 10
−12
, respectively. These MiniSTRs on the basis of high degree polymorphism and fragment size reduction will be highly informative in most of the forensic cases where DNA of the samples is degraded, mass disasters, and dead body identification. Significant differences were observed in all loci when comparing with same STR loci in previously published different world populations.</description><subject>DNA Fingerprinting</subject><subject>Forensic Medicine</subject><subject>Gene Frequency</subject><subject>Genetic testing</subject><subject>Genetics, Population</subject><subject>Humans</subject><subject>Medical Law</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microsatellite Repeats</subject><subject>Molecular biology</subject><subject>Molecular weight</subject><subject>Original Article</subject><subject>Pakistan</subject><subject>Paternity</subject><subject>Polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Thermal cycling</subject><issn>0937-9827</issn><issn>1437-1596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkUFLHjEQhoNU9FP7A3opgV68bM1kk83mWKS1gqKoPYdskq35uptsk92D_95sP1tKoXgYZmCeeUN4EHoH5CMQIs4yIQxYRUoBUFHxPbQBVosKuGzeoA2RZZYtFYfoKOctISAawQ_QIeWMUsKbDeovXHCzN3iKw9MY0_To84hjj2cX8LUP_v7hDg_ReOwDnh9d4aZl0LOPYaVul7DVHb7VP3yedcB9TGu5kEuknqbBm1_sCdrv9ZDd25d-jL59-fxw_rW6urm4PP90VRkm-Fx1xnDbUdJ2WogOTGdANoJy3tdWWFsb2QtrRFc7S3rJpG2sBUu5ptwCsVAfo9Nd7pTiz8XlWY0-GzcMOri4ZAUtl6ylddO8jgrSQlODpAX98A-6jUsK5SMrJZhkpRUKdpRJMefkejUlP-r0pICo1Zfa-VLFl1p9KV5u3r8kL93o7J-L34IKQHdALqvw3aW_nv5v6jOHZKBr</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Shafique, Muhammad</creator><creator>Shahzad, Muhammad Saqib</creator><creator>Shan, Muhammad Adnan</creator><creator>Ali, Azam</creator><creator>Rahman, Ziaur</creator><creator>Husnain, Tayyab</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AM</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BGRYB</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K7.</scope><scope>K9.</scope><scope>L6V</scope><scope>M0O</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150901</creationdate><title>Genetic polymorphism of ten MiniSTR loci in the population of Punjab Pakistan for forensic application</title><author>Shafique, Muhammad ; 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−12
, respectively. These MiniSTRs on the basis of high degree polymorphism and fragment size reduction will be highly informative in most of the forensic cases where DNA of the samples is degraded, mass disasters, and dead body identification. Significant differences were observed in all loci when comparing with same STR loci in previously published different world populations.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25422056</pmid><doi>10.1007/s00414-014-1127-5</doi><tpages>6</tpages></addata></record> |
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| subjects | DNA Fingerprinting Forensic Medicine Gene Frequency Genetic testing Genetics, Population Humans Medical Law Medicine Medicine & Public Health Microsatellite Repeats Molecular biology Molecular weight Original Article Pakistan Paternity Polymorphism Polymorphism, Genetic Real-Time Polymerase Chain Reaction Thermal cycling |
| title | Genetic polymorphism of ten MiniSTR loci in the population of Punjab Pakistan for forensic application |
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