Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization
Objective: Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens. Study Design: We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent in situ hybridization (FISH), a universal bacterial probe together with species-...
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| Veröffentlicht in: | Journal of perinatology 2017-01, Vol.37 (1), p.67-72 |
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| creator | Heida, F H Harmsen, H J M Timmer, A Kooi, E M W Bos, A F Hulscher, J B F |
| description | Objective:
Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens.
Study Design:
We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent
in situ
hybridization (FISH), a universal bacterial probe together with species-specific probes for
Clostridium
spp.,
Enterobacteriaceae
, bacteroides and enterococci/lactobacilli. We used a FISH scoring system to reveal invasion of the intestinal wall, in which 1 represented no colonies and 4 invasion of the intestinal wall.
Results:
We observed invasion of the intestinal wall in 22/43 of the most affected NEC tissue samples as compared with 16/43 in the least affected NEC tissue samples (
P
=0.03). A FISH score of 4 was reached in 7/43 control cases.
Enterobacteriaceae
dominated the NEC specimens.
Clostridium
spp. were detected occasionally in NEC samples.
Conclusion:
Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC tissue samples or controls.
Enterobacteriaceae
are prevalent in advanced NEC. |
| doi_str_mv | 10.1038/jp.2016.165 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859474436</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476578636</galeid><sourcerecordid>A476578636</sourcerecordid><originalsourceid>FETCH-LOGICAL-c485t-e5c56583743d29314d4d1c1cdec9fee557752e22a5bfa3f1897df8fb33b2399e3</originalsourceid><addsrcrecordid>eNqNks9rFDEUx4Modq2evMuAIIKddTJJJpljKbYWCr3oOWQyL7tZZpIxyQjtX29mt9W29CA5BPI-78c374vQe1ytcUXE1920rivcrHHDXqAVprwpGaPkJVpVnJJSENocoTcx7qpqCfLX6KjmjaAU8xWKlz24ZI3VKlnvCm-KTukEwaqhsO63isurdYUDHXyyt9ZtipwBwWs_2GRjESfQdgQXizkuUTPMPkDUmVoSo01zsb3pgu3t7b7JW_TKqCHCu7v7GP08__bj7Ht5dX1xeXZ6VWoqWCqBadYwQbKIvm4Jpj3tsca6B90aAMY4ZzXUtWKdUcRg0fLeCNMR0tWkbYEco8-HulPwv2aISY42jzUMyoGfo8SCtZRTSpr_QRmpWyGqjH58gu78HFwWsqcqtgz8j9qoAaR1xqeg9FJUnuYlMC6afdv1M1Q-PYxWewfG5vdHCZ8eJGxBDWkb_TAv3xofg18OYN5ajAGMnIIdVbiRuJKLbeRukottZLZNpj_caZq7Efq_7L1PMnByAGIOuQ2EB6KfqfcHT6fLhA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1855053743</pqid></control><display><type>article</type><title>Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Heida, F H ; Harmsen, H J M ; Timmer, A ; Kooi, E M W ; Bos, A F ; Hulscher, J B F</creator><creatorcontrib>Heida, F H ; Harmsen, H J M ; Timmer, A ; Kooi, E M W ; Bos, A F ; Hulscher, J B F</creatorcontrib><description>Objective:
Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens.
Study Design:
We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent
in situ
hybridization (FISH), a universal bacterial probe together with species-specific probes for
Clostridium
spp.,
Enterobacteriaceae
, bacteroides and enterococci/lactobacilli. We used a FISH scoring system to reveal invasion of the intestinal wall, in which 1 represented no colonies and 4 invasion of the intestinal wall.
Results:
We observed invasion of the intestinal wall in 22/43 of the most affected NEC tissue samples as compared with 16/43 in the least affected NEC tissue samples (
P
=0.03). A FISH score of 4 was reached in 7/43 control cases.
Enterobacteriaceae
dominated the NEC specimens.
Clostridium
spp. were detected occasionally in NEC samples.
Conclusion:
Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC tissue samples or controls.
Enterobacteriaceae
are prevalent in advanced NEC.</description><identifier>ISSN: 0743-8346</identifier><identifier>EISSN: 1476-5543</identifier><identifier>DOI: 10.1038/jp.2016.165</identifier><identifier>PMID: 27684417</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>692/308/575 ; 692/420/256/2516 ; 96 ; 96/34 ; 96/35 ; Age ; Autopsies ; Bacteria ; Bacteroides ; Birth weight ; Breastfeeding & lactation ; Case-Control Studies ; Clostridium ; Clostridium - isolation & purification ; Diagnosis ; Enterobacteriaceae ; Enterobacteriaceae - isolation & purification ; Enterocolitis, Necrotizing - pathology ; Enterocolitis, Necrotizing - surgery ; Female ; Fluorescence in situ hybridization ; Hospitals ; Humans ; Hybridization ; In Situ Hybridization, Fluorescence ; Infant, Newborn ; Intestines - microbiology ; Intestines - pathology ; Lactobacillus ; Male ; Medicine ; Medicine & Public Health ; Microbiota ; Necrotizing enterocolitis ; Neonatal care ; Netherlands ; Newborn babies ; original-article ; Pathology ; Pediatric Surgery ; Pediatrics ; Premature babies ; Retrospective Studies ; Risk factors ; Surgery ; Tertiary Care Centers</subject><ispartof>Journal of perinatology, 2017-01, Vol.37 (1), p.67-72</ispartof><rights>Nature America, Inc., part of Springer Nature. 2017</rights><rights>COPYRIGHT 2017 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-e5c56583743d29314d4d1c1cdec9fee557752e22a5bfa3f1897df8fb33b2399e3</citedby><cites>FETCH-LOGICAL-c485t-e5c56583743d29314d4d1c1cdec9fee557752e22a5bfa3f1897df8fb33b2399e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/jp.2016.165$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/jp.2016.165$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27684417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heida, F H</creatorcontrib><creatorcontrib>Harmsen, H J M</creatorcontrib><creatorcontrib>Timmer, A</creatorcontrib><creatorcontrib>Kooi, E M W</creatorcontrib><creatorcontrib>Bos, A F</creatorcontrib><creatorcontrib>Hulscher, J B F</creatorcontrib><title>Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization</title><title>Journal of perinatology</title><addtitle>J Perinatol</addtitle><addtitle>J Perinatol</addtitle><description>Objective:
Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens.
Study Design:
We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent
in situ
hybridization (FISH), a universal bacterial probe together with species-specific probes for
Clostridium
spp.,
Enterobacteriaceae
, bacteroides and enterococci/lactobacilli. We used a FISH scoring system to reveal invasion of the intestinal wall, in which 1 represented no colonies and 4 invasion of the intestinal wall.
Results:
We observed invasion of the intestinal wall in 22/43 of the most affected NEC tissue samples as compared with 16/43 in the least affected NEC tissue samples (
P
=0.03). A FISH score of 4 was reached in 7/43 control cases.
Enterobacteriaceae
dominated the NEC specimens.
Clostridium
spp. were detected occasionally in NEC samples.
Conclusion:
Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC tissue samples or controls.
Enterobacteriaceae
are prevalent in advanced NEC.</description><subject>692/308/575</subject><subject>692/420/256/2516</subject><subject>96</subject><subject>96/34</subject><subject>96/35</subject><subject>Age</subject><subject>Autopsies</subject><subject>Bacteria</subject><subject>Bacteroides</subject><subject>Birth weight</subject><subject>Breastfeeding & lactation</subject><subject>Case-Control Studies</subject><subject>Clostridium</subject><subject>Clostridium - isolation & purification</subject><subject>Diagnosis</subject><subject>Enterobacteriaceae</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>Enterocolitis, Necrotizing - pathology</subject><subject>Enterocolitis, Necrotizing - surgery</subject><subject>Female</subject><subject>Fluorescence in situ hybridization</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hybridization</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Infant, Newborn</subject><subject>Intestines - microbiology</subject><subject>Intestines - pathology</subject><subject>Lactobacillus</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microbiota</subject><subject>Necrotizing enterocolitis</subject><subject>Neonatal care</subject><subject>Netherlands</subject><subject>Newborn babies</subject><subject>original-article</subject><subject>Pathology</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Premature babies</subject><subject>Retrospective Studies</subject><subject>Risk factors</subject><subject>Surgery</subject><subject>Tertiary Care Centers</subject><issn>0743-8346</issn><issn>1476-5543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNks9rFDEUx4Modq2evMuAIIKddTJJJpljKbYWCr3oOWQyL7tZZpIxyQjtX29mt9W29CA5BPI-78c374vQe1ytcUXE1920rivcrHHDXqAVprwpGaPkJVpVnJJSENocoTcx7qpqCfLX6KjmjaAU8xWKlz24ZI3VKlnvCm-KTukEwaqhsO63isurdYUDHXyyt9ZtipwBwWs_2GRjESfQdgQXizkuUTPMPkDUmVoSo01zsb3pgu3t7b7JW_TKqCHCu7v7GP08__bj7Ht5dX1xeXZ6VWoqWCqBadYwQbKIvm4Jpj3tsca6B90aAMY4ZzXUtWKdUcRg0fLeCNMR0tWkbYEco8-HulPwv2aISY42jzUMyoGfo8SCtZRTSpr_QRmpWyGqjH58gu78HFwWsqcqtgz8j9qoAaR1xqeg9FJUnuYlMC6afdv1M1Q-PYxWewfG5vdHCZ8eJGxBDWkb_TAv3xofg18OYN5ajAGMnIIdVbiRuJKLbeRukottZLZNpj_caZq7Efq_7L1PMnByAGIOuQ2EB6KfqfcHT6fLhA</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Heida, F H</creator><creator>Harmsen, H J M</creator><creator>Timmer, A</creator><creator>Kooi, E M W</creator><creator>Bos, A F</creator><creator>Hulscher, J B F</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7RV</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20170101</creationdate><title>Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization</title><author>Heida, F H ; Harmsen, H J M ; Timmer, A ; Kooi, E M W ; Bos, A F ; Hulscher, J B F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-e5c56583743d29314d4d1c1cdec9fee557752e22a5bfa3f1897df8fb33b2399e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>692/308/575</topic><topic>692/420/256/2516</topic><topic>96</topic><topic>96/34</topic><topic>96/35</topic><topic>Age</topic><topic>Autopsies</topic><topic>Bacteria</topic><topic>Bacteroides</topic><topic>Birth weight</topic><topic>Breastfeeding & lactation</topic><topic>Case-Control Studies</topic><topic>Clostridium</topic><topic>Clostridium - isolation & purification</topic><topic>Diagnosis</topic><topic>Enterobacteriaceae</topic><topic>Enterobacteriaceae - isolation & purification</topic><topic>Enterocolitis, Necrotizing - pathology</topic><topic>Enterocolitis, Necrotizing - surgery</topic><topic>Female</topic><topic>Fluorescence in situ hybridization</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Hybridization</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Infant, Newborn</topic><topic>Intestines - microbiology</topic><topic>Intestines - pathology</topic><topic>Lactobacillus</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microbiota</topic><topic>Necrotizing enterocolitis</topic><topic>Neonatal care</topic><topic>Netherlands</topic><topic>Newborn babies</topic><topic>original-article</topic><topic>Pathology</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Premature babies</topic><topic>Retrospective Studies</topic><topic>Risk factors</topic><topic>Surgery</topic><topic>Tertiary Care Centers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heida, F H</creatorcontrib><creatorcontrib>Harmsen, H J M</creatorcontrib><creatorcontrib>Timmer, A</creatorcontrib><creatorcontrib>Kooi, E M W</creatorcontrib><creatorcontrib>Bos, A F</creatorcontrib><creatorcontrib>Hulscher, J B F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of perinatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heida, F H</au><au>Harmsen, H J M</au><au>Timmer, A</au><au>Kooi, E M W</au><au>Bos, A F</au><au>Hulscher, J B F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization</atitle><jtitle>Journal of perinatology</jtitle><stitle>J Perinatol</stitle><addtitle>J Perinatol</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>37</volume><issue>1</issue><spage>67</spage><epage>72</epage><pages>67-72</pages><issn>0743-8346</issn><eissn>1476-5543</eissn><abstract>Objective:
Investigation of bacterial invasion into the intestinal wall in necrotizing enterocolitis (NEC) specimens.
Study Design:
We compared 43 surgical NEC specimens with 43 age-matched controls. We used fluorescent
in situ
hybridization (FISH), a universal bacterial probe together with species-specific probes for
Clostridium
spp.,
Enterobacteriaceae
, bacteroides and enterococci/lactobacilli. We used a FISH scoring system to reveal invasion of the intestinal wall, in which 1 represented no colonies and 4 invasion of the intestinal wall.
Results:
We observed invasion of the intestinal wall in 22/43 of the most affected NEC tissue samples as compared with 16/43 in the least affected NEC tissue samples (
P
=0.03). A FISH score of 4 was reached in 7/43 control cases.
Enterobacteriaceae
dominated the NEC specimens.
Clostridium
spp. were detected occasionally in NEC samples.
Conclusion:
Bacterial invasion of the intestinal wall is more present in most affected NEC tissue samples compared with least affected NEC tissue samples or controls.
Enterobacteriaceae
are prevalent in advanced NEC.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>27684417</pmid><doi>10.1038/jp.2016.165</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of perinatology, 2017-01, Vol.37 (1), p.67-72 |
| issn | 0743-8346 1476-5543 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | 692/308/575 692/420/256/2516 96 96/34 96/35 Age Autopsies Bacteria Bacteroides Birth weight Breastfeeding & lactation Case-Control Studies Clostridium Clostridium - isolation & purification Diagnosis Enterobacteriaceae Enterobacteriaceae - isolation & purification Enterocolitis, Necrotizing - pathology Enterocolitis, Necrotizing - surgery Female Fluorescence in situ hybridization Hospitals Humans Hybridization In Situ Hybridization, Fluorescence Infant, Newborn Intestines - microbiology Intestines - pathology Lactobacillus Male Medicine Medicine & Public Health Microbiota Necrotizing enterocolitis Neonatal care Netherlands Newborn babies original-article Pathology Pediatric Surgery Pediatrics Premature babies Retrospective Studies Risk factors Surgery Tertiary Care Centers |
| title | Identification of bacterial invasion in necrotizing enterocolitis specimens using fluorescent in situ hybridization |
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