Conditioning to ethanol in the fruit fly—a study using an inhibitor of ADH
To identify processes involved in the choice of ethanol by adult Drosophila, flies homozygous Adh F, reared in the absence of alcohol were placed in contact with: a) an ethanol-free medium, b) a medium containing ethanol, c) a medium supplemented with 4-methylpyrazole (4-MP, an inhibitor of the ADH...
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Veröffentlicht in: | Journal of insect physiology 1999-06, Vol.45 (6), p.579-586 |
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creator | Cadieu, N. Cadieu, J-C. El Ghadraoui, L. Grimal, A. Lambœuf, Y. |
description | To identify processes involved in the choice of ethanol by adult Drosophila, flies homozygous Adh
F, reared in the absence of alcohol were placed in contact with: a) an ethanol-free medium, b) a medium containing ethanol, c) a medium supplemented with 4-methylpyrazole (4-MP, an inhibitor of the ADH pathway), d) a medium containing ethanol and 4-MP. The choice of ethanol over a medium without ethanol was evaluated by measuring the duration of extension of the proboscis of the flies in each of the media. A slight preference for the ethanol-supplemented medium was observed in the naive flies, which was enhanced by previous exposure to ethanol. Exposure to ethanol and 4-MP, however, led to an avoidance of ethanol. There was a reduction in ADH activity on treatment of the flies with 4-MP, and signs of malaise (reduced locomotor activity, loss of balance) were observed in the flies who ingested both ethanol and inhibitor. We concluded that the preference for ethanol stems from an associative learning related to ethanol utilization. Inhibition of enzymes of ADH pathway led to a conditioned aversion due to disturbance of ethanol metabolism giving rise to malaise. |
doi_str_mv | 10.1016/S0022-1910(99)00041-4 |
format | Article |
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F, reared in the absence of alcohol were placed in contact with: a) an ethanol-free medium, b) a medium containing ethanol, c) a medium supplemented with 4-methylpyrazole (4-MP, an inhibitor of the ADH pathway), d) a medium containing ethanol and 4-MP. The choice of ethanol over a medium without ethanol was evaluated by measuring the duration of extension of the proboscis of the flies in each of the media. A slight preference for the ethanol-supplemented medium was observed in the naive flies, which was enhanced by previous exposure to ethanol. Exposure to ethanol and 4-MP, however, led to an avoidance of ethanol. There was a reduction in ADH activity on treatment of the flies with 4-MP, and signs of malaise (reduced locomotor activity, loss of balance) were observed in the flies who ingested both ethanol and inhibitor. We concluded that the preference for ethanol stems from an associative learning related to ethanol utilization. Inhibition of enzymes of ADH pathway led to a conditioned aversion due to disturbance of ethanol metabolism giving rise to malaise.</description><subject>Adh</subject><subject>alcohol dehydrogenase</subject><subject>animal behavior</subject><subject>associative learning</subject><subject>Aversive conditioning</subject><subject>conditioning</subject><subject>culture media</subject><subject>Drosophila melanogaster</subject><subject>enzyme activity</subject><subject>enzyme inhibitors</subject><subject>ethanol</subject><subject>Ethanol preference</subject><subject>feeding preferences</subject><subject>Inhibitor</subject><subject>learning</subject><subject>r-methylpyrazole</subject><subject>Tephritidae</subject><issn>0022-1910</issn><issn>1879-1611</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqF0EFvFCEUwHFibOy2-hFUTqYepr7HMDNwapqt2iab9FB7JswAXczsUIEx2Zsfwk_oJ5HtbvSmCQmX3-ORPyGvEc4RsP1wB8BYhRLhTMr3AMCx4s_IAkUnK2wRn5PFH3JMTlL6WlDTiuYFOUbWdVDzekFWyzAZn32Y_PRAc6A2r_UURuonmteWujj7TN24_fXjp6Ypz2ZL57Szeipm7XufQ6TB0cur65fkyOkx2VeH-5Tcf_r4ZXldrW4_3ywvV9XAUeSqr7VxTA68dX0L4LqhHBRWGGd77lDUNUrTcGAgCinWCNFjz2rk2nZtfUrO9u8-xvBttimrjU-DHUc92TAnhaKRHCQDXui7f9OONbJtuwKbPRxiSClapx6j3-i4VQhqV1w9FVe7nEpK9VRc7Ra8OSyY-401f6cOiQt4uwdOB6Ufok_q_o4B1sBkDbxhRVzshS3NvnsbVRq8nQZrfLRDVib4_3ziN-_smSc</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Cadieu, N.</creator><creator>Cadieu, J-C.</creator><creator>El Ghadraoui, L.</creator><creator>Grimal, A.</creator><creator>Lambœuf, Y.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Conditioning to ethanol in the fruit fly—a study using an inhibitor of ADH</title><author>Cadieu, N. ; Cadieu, J-C. ; El Ghadraoui, L. ; Grimal, A. ; Lambœuf, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-b3adf29c46fb600f7cf7c18e8dfeb4f183319d5402086fbdf2d88b1b2314ae763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adh</topic><topic>alcohol dehydrogenase</topic><topic>animal behavior</topic><topic>associative learning</topic><topic>Aversive conditioning</topic><topic>conditioning</topic><topic>culture media</topic><topic>Drosophila melanogaster</topic><topic>enzyme activity</topic><topic>enzyme inhibitors</topic><topic>ethanol</topic><topic>Ethanol preference</topic><topic>feeding preferences</topic><topic>Inhibitor</topic><topic>learning</topic><topic>r-methylpyrazole</topic><topic>Tephritidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cadieu, N.</creatorcontrib><creatorcontrib>Cadieu, J-C.</creatorcontrib><creatorcontrib>El Ghadraoui, L.</creatorcontrib><creatorcontrib>Grimal, A.</creatorcontrib><creatorcontrib>Lambœuf, Y.</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of insect physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cadieu, N.</au><au>Cadieu, J-C.</au><au>El Ghadraoui, L.</au><au>Grimal, A.</au><au>Lambœuf, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conditioning to ethanol in the fruit fly—a study using an inhibitor of ADH</atitle><jtitle>Journal of insect physiology</jtitle><addtitle>J Insect Physiol</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>45</volume><issue>6</issue><spage>579</spage><epage>586</epage><pages>579-586</pages><issn>0022-1910</issn><eissn>1879-1611</eissn><abstract>To identify processes involved in the choice of ethanol by adult Drosophila, flies homozygous Adh
F, reared in the absence of alcohol were placed in contact with: a) an ethanol-free medium, b) a medium containing ethanol, c) a medium supplemented with 4-methylpyrazole (4-MP, an inhibitor of the ADH pathway), d) a medium containing ethanol and 4-MP. The choice of ethanol over a medium without ethanol was evaluated by measuring the duration of extension of the proboscis of the flies in each of the media. A slight preference for the ethanol-supplemented medium was observed in the naive flies, which was enhanced by previous exposure to ethanol. Exposure to ethanol and 4-MP, however, led to an avoidance of ethanol. There was a reduction in ADH activity on treatment of the flies with 4-MP, and signs of malaise (reduced locomotor activity, loss of balance) were observed in the flies who ingested both ethanol and inhibitor. We concluded that the preference for ethanol stems from an associative learning related to ethanol utilization. Inhibition of enzymes of ADH pathway led to a conditioned aversion due to disturbance of ethanol metabolism giving rise to malaise.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>12770343</pmid><doi>10.1016/S0022-1910(99)00041-4</doi><tpages>8</tpages></addata></record> |
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subjects | Adh alcohol dehydrogenase animal behavior associative learning Aversive conditioning conditioning culture media Drosophila melanogaster enzyme activity enzyme inhibitors ethanol Ethanol preference feeding preferences Inhibitor learning r-methylpyrazole Tephritidae |
title | Conditioning to ethanol in the fruit fly—a study using an inhibitor of ADH |
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