Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance
Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment s...
Gespeichert in:
| Veröffentlicht in: | Theoretical and applied genetics 2002-05, Vol.104 (6-7), p.1055-1063 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1063 |
|---|---|
| container_issue | 6-7 |
| container_start_page | 1055 |
| container_title | Theoretical and applied genetics |
| container_volume | 104 |
| creator | BROTMAN, Y SILBERSTEIN, L KOVALSKI, I PERIN, C DOGIMONT, C PITRAT, M KLINGLER, J THOMPSON, G. A PERL-TREVES, R |
| description | Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporum race 1, F. oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed. |
| doi_str_mv | 10.1007/s00122-001-0808-x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859396268</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A301086239</galeid><sourcerecordid>A301086239</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-c21edc5f281a1a9a9129c5bd3ff9deafd240425b00ae2b60bbcb8ed5d4d01a0b3</originalsourceid><addsrcrecordid>eNqF0cFu1DAQBmALgei28ABckAUCwSEwHidZ51hVBSpVQipwthx7Elyy9mIn0vL2eNkVVblwsS_f_PL4Z-yZgHcCYP0-AwjEqpwVKFDV7gFbiVpihVjjQ7YCqKFq1g2esNOcbwEAG5CP2YnARmErcMXohrLPswmW-EiB-Pe4iVMcF8rcB76hKQZuEvHJhx_k-Bz_sNlbPkXruY1hoJR8GLnzmUwmboLjW8ozT3-jn7BHg5kyPT3eZ-zbh8uvF5-q688fry7Orytb191cWRTkbDOgEkaYznQCO9v0Tg5D58gMDmuosekBDGHfQt_bXpFrXO1AGOjlGXtzyN2m-LOsMOuNz5amyQSKS9ZCNZ3sWmxVoa__Q6UEqeoCX_wDb-OSQllDr1FCScO2oJcHNJqJtA9DnJOx-0R9LkGAalF2Rb29p8rnzbSbR7PkrK--3Ny34mBtijknGvQ2-Y1Jv7QAvW9fH9rX5dT79vWuzDw_PnXpN-TuJo51F_DqCEy2ZhpSKcfnOyfbVinVyd9vhLZ2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>723096226</pqid></control><display><type>article</type><title>Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance</title><source>SpringerLink Journals - AutoHoldings</source><creator>BROTMAN, Y ; SILBERSTEIN, L ; KOVALSKI, I ; PERIN, C ; DOGIMONT, C ; PITRAT, M ; KLINGLER, J ; THOMPSON, G. A ; PERL-TREVES, R</creator><creatorcontrib>BROTMAN, Y ; SILBERSTEIN, L ; KOVALSKI, I ; PERIN, C ; DOGIMONT, C ; PITRAT, M ; KLINGLER, J ; THOMPSON, G. A ; PERL-TREVES, R</creatorcontrib><description>Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporum race 1, F. oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-001-0808-x</identifier><identifier>PMID: 12582612</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Aphis gossypii ; Biological and medical sciences ; Classical genetics, quantitative genetics, hybrids ; Cloning ; Cucumis melo ; Disease ; Fundamental and applied biological sciences. Psychology ; Fusarium oxysporum ; Gene loci ; Genetic aspects ; Genetics of eukaryotes. Biological and molecular evolution ; Health aspects ; Kinases ; Melons ; NBS-LRR gene ; Papaya ringspot virus ; Pathogens ; Perl ; Physiological aspects ; Plant defenses ; Plant genetics ; Plant immunology ; Proteins ; Pteridophyta, spermatophyta ; Signal transduction ; Vegetals</subject><ispartof>Theoretical and applied genetics, 2002-05, Vol.104 (6-7), p.1055-1063</ispartof><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 Springer</rights><rights>Springer-Verlag 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-c21edc5f281a1a9a9129c5bd3ff9deafd240425b00ae2b60bbcb8ed5d4d01a0b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13668889$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12582612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BROTMAN, Y</creatorcontrib><creatorcontrib>SILBERSTEIN, L</creatorcontrib><creatorcontrib>KOVALSKI, I</creatorcontrib><creatorcontrib>PERIN, C</creatorcontrib><creatorcontrib>DOGIMONT, C</creatorcontrib><creatorcontrib>PITRAT, M</creatorcontrib><creatorcontrib>KLINGLER, J</creatorcontrib><creatorcontrib>THOMPSON, G. A</creatorcontrib><creatorcontrib>PERL-TREVES, R</creatorcontrib><title>Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporum race 1, F. oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed.</description><subject>Aphis gossypii</subject><subject>Biological and medical sciences</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cloning</subject><subject>Cucumis melo</subject><subject>Disease</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium oxysporum</subject><subject>Gene loci</subject><subject>Genetic aspects</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Health aspects</subject><subject>Kinases</subject><subject>Melons</subject><subject>NBS-LRR gene</subject><subject>Papaya ringspot virus</subject><subject>Pathogens</subject><subject>Perl</subject><subject>Physiological aspects</subject><subject>Plant defenses</subject><subject>Plant genetics</subject><subject>Plant immunology</subject><subject>Proteins</subject><subject>Pteridophyta, spermatophyta</subject><subject>Signal transduction</subject><subject>Vegetals</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0cFu1DAQBmALgei28ABckAUCwSEwHidZ51hVBSpVQipwthx7Elyy9mIn0vL2eNkVVblwsS_f_PL4Z-yZgHcCYP0-AwjEqpwVKFDV7gFbiVpihVjjQ7YCqKFq1g2esNOcbwEAG5CP2YnARmErcMXohrLPswmW-EiB-Pe4iVMcF8rcB76hKQZuEvHJhx_k-Bz_sNlbPkXruY1hoJR8GLnzmUwmboLjW8ozT3-jn7BHg5kyPT3eZ-zbh8uvF5-q688fry7Orytb191cWRTkbDOgEkaYznQCO9v0Tg5D58gMDmuosekBDGHfQt_bXpFrXO1AGOjlGXtzyN2m-LOsMOuNz5amyQSKS9ZCNZ3sWmxVoa__Q6UEqeoCX_wDb-OSQllDr1FCScO2oJcHNJqJtA9DnJOx-0R9LkGAalF2Rb29p8rnzbSbR7PkrK--3Ny34mBtijknGvQ2-Y1Jv7QAvW9fH9rX5dT79vWuzDw_PnXpN-TuJo51F_DqCEy2ZhpSKcfnOyfbVinVyd9vhLZ2</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>BROTMAN, Y</creator><creator>SILBERSTEIN, L</creator><creator>KOVALSKI, I</creator><creator>PERIN, C</creator><creator>DOGIMONT, C</creator><creator>PITRAT, M</creator><creator>KLINGLER, J</creator><creator>THOMPSON, G. A</creator><creator>PERL-TREVES, R</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance</title><author>BROTMAN, Y ; SILBERSTEIN, L ; KOVALSKI, I ; PERIN, C ; DOGIMONT, C ; PITRAT, M ; KLINGLER, J ; THOMPSON, G. A ; PERL-TREVES, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-c21edc5f281a1a9a9129c5bd3ff9deafd240425b00ae2b60bbcb8ed5d4d01a0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aphis gossypii</topic><topic>Biological and medical sciences</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cloning</topic><topic>Cucumis melo</topic><topic>Disease</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusarium oxysporum</topic><topic>Gene loci</topic><topic>Genetic aspects</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Health aspects</topic><topic>Kinases</topic><topic>Melons</topic><topic>NBS-LRR gene</topic><topic>Papaya ringspot virus</topic><topic>Pathogens</topic><topic>Perl</topic><topic>Physiological aspects</topic><topic>Plant defenses</topic><topic>Plant genetics</topic><topic>Plant immunology</topic><topic>Proteins</topic><topic>Pteridophyta, spermatophyta</topic><topic>Signal transduction</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BROTMAN, Y</creatorcontrib><creatorcontrib>SILBERSTEIN, L</creatorcontrib><creatorcontrib>KOVALSKI, I</creatorcontrib><creatorcontrib>PERIN, C</creatorcontrib><creatorcontrib>DOGIMONT, C</creatorcontrib><creatorcontrib>PITRAT, M</creatorcontrib><creatorcontrib>KLINGLER, J</creatorcontrib><creatorcontrib>THOMPSON, G. A</creatorcontrib><creatorcontrib>PERL-TREVES, R</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BROTMAN, Y</au><au>SILBERSTEIN, L</au><au>KOVALSKI, I</au><au>PERIN, C</au><au>DOGIMONT, C</au><au>PITRAT, M</au><au>KLINGLER, J</au><au>THOMPSON, G. A</au><au>PERL-TREVES, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>104</volume><issue>6-7</issue><spage>1055</spage><epage>1063</epage><pages>1055-1063</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporum race 1, F. oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>12582612</pmid><doi>10.1007/s00122-001-0808-x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0040-5752 |
| ispartof | Theoretical and applied genetics, 2002-05, Vol.104 (6-7), p.1055-1063 |
| issn | 0040-5752 1432-2242 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1859396268 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Aphis gossypii Biological and medical sciences Classical genetics, quantitative genetics, hybrids Cloning Cucumis melo Disease Fundamental and applied biological sciences. Psychology Fusarium oxysporum Gene loci Genetic aspects Genetics of eukaryotes. Biological and molecular evolution Health aspects Kinases Melons NBS-LRR gene Papaya ringspot virus Pathogens Perl Physiological aspects Plant defenses Plant genetics Plant immunology Proteins Pteridophyta, spermatophyta Signal transduction Vegetals |
| title | Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T23%3A57%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Resistance%20gene%20homologues%20in%20melon%20are%20linked%20to%20genetic%20loci%20conferring%20disease%20and%20pest%20resistance&rft.jtitle=Theoretical%20and%20applied%20genetics&rft.au=BROTMAN,%20Y&rft.date=2002-05-01&rft.volume=104&rft.issue=6-7&rft.spage=1055&rft.epage=1063&rft.pages=1055-1063&rft.issn=0040-5752&rft.eissn=1432-2242&rft.coden=THAGA6&rft_id=info:doi/10.1007/s00122-001-0808-x&rft_dat=%3Cgale_proqu%3EA301086239%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=723096226&rft_id=info:pmid/12582612&rft_galeid=A301086239&rfr_iscdi=true |