Restoration of the wild-type phenotype in Pichia guilliermondii transformants

Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transforman...

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Veröffentlicht in:	Microbiology (New York) 2002-05, Vol.71 (3), p.314-318
Hauptverfasser:	PINYAGA, Yu. V, PROKOPIV, T. M, PETRISHIN, A. V, KHALIMONCHUK, O. V, PROTCHENKO, O. V, FEDOROVICH, D. V, BORETSKY, Yu. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology GTP cyclohydrolase Microbiology Mycology Pichia guilliermondii
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Beschreibung
Zusammenfassung:	Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.
ISSN:	0026-2617 0026-3656 1608-3237
DOI:	10.1023/A:1015858712461