Immunolocalization of Parathyroid Hormone in Human Parathyroid Glands with Special References to Microwave Antigen Retrieval

Immunolocalization of Parathyroid Hormone in Human Parathyroid Glands with Special References to Microwave Antigen Retrieval

The subcellular localization of parathyroid hormone (PTH) in the normal human parathyroid glands with particular reference to microwave antigen retrieval was investigated using peroxidase-labeled PTH antibody, immunohistochemical, and immunoelectron microscopic methods. The results revealed that PTH...

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Veröffentlicht in:Endocrine pathology 1995-08, Vol.6 (3), p.223-227
Hauptverfasser: Utsunomiya, Hirotoshi, Shan, Liang, Kawano, Ichiro, Iwasaki, Arata, Ono, Kazuo, Kobayashi, Akira, Kuma, Kanji, Kishikawa, Seigo, Kakudo, Kennichi
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container_end_page 227
container_issue 3
container_start_page 223
container_title Endocrine pathology
container_volume 6
creator Utsunomiya, Hirotoshi
Shan, Liang
Kawano, Ichiro
Iwasaki, Arata
Ono, Kazuo
Kobayashi, Akira
Kuma, Kanji
Kishikawa, Seigo
Kakudo, Kennichi
description The subcellular localization of parathyroid hormone (PTH) in the normal human parathyroid glands with particular reference to microwave antigen retrieval was investigated using peroxidase-labeled PTH antibody, immunohistochemical, and immunoelectron microscopic methods. The results revealed that PTH granules existed mainly as pro-PTH on the trans side of Golgi and in the regions adjacent to Golgi apparatus. Only a small proportion of secretory granules were stored near the plasma membrane. Microwave irradiation was essential for the immunodetection of PTH. As the irradiative time extended from 1 to 30 min, the staining intensity increased, and the subcellular preservation decreased. Microwave irradiation for 15 mm (with the sections in citrate buffer) with a power output of 500 W is the most ideal for PTH antigen retrieval, as well as for subcellular preservation.
doi_str_mv 10.1007/BF02739886
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title Immunolocalization of Parathyroid Hormone in Human Parathyroid Glands with Special References to Microwave Antigen Retrieval
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