The search for proteins with immunochemical affinity to plant stress proteins at cold-adapted endemic Baikal fishes

The search for proteins with immunochemical affinity to plant stress proteins in endemic Baikal fishes shows the presence of proteins, immunochemically related to plant heat-stabile proteins and plant uncoupling protein CSP 310. Western blotting showed that among the native cytoplasmic proteins of e...

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Veröffentlicht in:Journal of thermal biology 2001-06, Vol.26 (3), p.209-214
Hauptverfasser: Ostroumova, E.A., Ostroumov, V.A., Sumina, O.N., Misharin, S.I., Antipina, A.I., Grabelnych, O.I., Zykova, V.V., Pobezhimova, T.P., Kolesnichenko, A.V.
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Sprache:eng
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Zusammenfassung:The search for proteins with immunochemical affinity to plant stress proteins in endemic Baikal fishes shows the presence of proteins, immunochemically related to plant heat-stabile proteins and plant uncoupling protein CSP 310. Western blotting showed that among the native cytoplasmic proteins of endemic Baikal fishes there are proteins immunochemically related to heat-stabile plant proteins with molecular weights about 480, 200–290, 150, 140 and about 90–100 kD. SDS-electrophoresis showed the presence of polypeptides with molecular weights 23, 17 and 14 kD in all species investigated and an additional 35 kD polypeptide in Cottocomephorus grewingki. The search for polypeptides with immunochemical affinity to plant stress uncoupling protein CSP 310 in endemic Baikal fishes shows the presence of a 14 kD polypeptide, immunochemically related to it.
ISSN:0306-4565
1879-0992
DOI:10.1016/S0306-4565(00)00044-9