Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers
Morphological, chemical and genetic differences of 12 tree basil ( Ocimum gratissimum L.) accessions were studied to determine whether volatile oils and flavonoids can be used as taxonomical markers and to examine the relationship between RAPDs to these chemical markers. Eugenol, thymol, and geranio...
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| Veröffentlicht in: | Biochemical systematics and ecology 2001-03, Vol.29 (3), p.287-304 |
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| creator | Vieira, Roberto F Grayer, Renée J Paton, Alan Simon, James E |
| description | Morphological, chemical and genetic differences of 12 tree basil (
Ocimum gratissimum L.) accessions were studied to determine whether volatile oils and flavonoids can be used as taxonomical markers and to examine the relationship between RAPDs to these chemical markers. Eugenol, thymol, and geraniol were the major volatile oil constituents found in
Ocimum gratissimum. Xantomicrol and cirsimaritin were the major external flavones. The accessions morphologically described as
O. gratissimum var
. gratissimum contained eugenol as the major volatile oil constituent, and cirsimaritin as the major flavone.
Ocimum gratissimum var.
macrophyllum accessions contained thymol as the major volatile oil constituent, and xantomicrol as the major flavone. A distinct essential oil and flavone chemotype (producing geraniol and a mixture of the flavones cirsimaritin, isothymusin, xanthomicrol, and luteolin) was found in an accession genetically more distant from the other two groups when analyzed by molecular markers. The accessions could be divided based on volatile oil constituents into six groups: (1) thymol:
α-copaene (ot24, ot25, ot26, and ot28); (2) eugenol:spathulenol (ot17, ot63, and ot52); (3) thymol:
p-cymene (ot65); (4) eugenol:
γ-muurolene (ot27 and ot29); (5) eugenol:thymol: spathulenol (ot85); and (6) geraniol (ot84). Cluster analysis of RAPD markers showed that there are three groups that are distinct genetically and highly correlated (
r=0.814) to volatile oil constituents. |
| doi_str_mv | 10.1016/S0305-1978(00)00062-4 |
| format | Article |
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Ocimum gratissimum L.) accessions were studied to determine whether volatile oils and flavonoids can be used as taxonomical markers and to examine the relationship between RAPDs to these chemical markers. Eugenol, thymol, and geraniol were the major volatile oil constituents found in
Ocimum gratissimum. Xantomicrol and cirsimaritin were the major external flavones. The accessions morphologically described as
O. gratissimum var
. gratissimum contained eugenol as the major volatile oil constituent, and cirsimaritin as the major flavone.
Ocimum gratissimum var.
macrophyllum accessions contained thymol as the major volatile oil constituent, and xantomicrol as the major flavone. A distinct essential oil and flavone chemotype (producing geraniol and a mixture of the flavones cirsimaritin, isothymusin, xanthomicrol, and luteolin) was found in an accession genetically more distant from the other two groups when analyzed by molecular markers. The accessions could be divided based on volatile oil constituents into six groups: (1) thymol:
α-copaene (ot24, ot25, ot26, and ot28); (2) eugenol:spathulenol (ot17, ot63, and ot52); (3) thymol:
p-cymene (ot65); (4) eugenol:
γ-muurolene (ot27 and ot29); (5) eugenol:thymol: spathulenol (ot85); and (6) geraniol (ot84). Cluster analysis of RAPD markers showed that there are three groups that are distinct genetically and highly correlated (
r=0.814) to volatile oil constituents.</description><identifier>ISSN: 0305-1978</identifier><identifier>EISSN: 1873-2925</identifier><identifier>DOI: 10.1016/S0305-1978(00)00062-4</identifier><identifier>PMID: 11152947</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>a-copaene ; basil ; chemotypes ; Cirsimaritin ; cluster analysis ; Essential oils ; Eugenol ; External flavones ; flavonoids ; g-muurolene ; genetic markers ; genetic variation ; Geraniol ; Lamiaceae ; luteolin ; Molecular markers ; Ocimum gratissimum ; oils ; p-cymene ; random amplified polymorphic DNA technique ; spathulenol ; Thymol ; Tree basil ; Xantomicrol</subject><ispartof>Biochemical systematics and ecology, 2001-03, Vol.29 (3), p.287-304</ispartof><rights>2001 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-42d4b1272b046f1acd0e57c301f77fd20525348729125c10652816ca04e47bfd3</citedby><cites>FETCH-LOGICAL-c484t-42d4b1272b046f1acd0e57c301f77fd20525348729125c10652816ca04e47bfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0305-1978(00)00062-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11152947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vieira, Roberto F</creatorcontrib><creatorcontrib>Grayer, Renée J</creatorcontrib><creatorcontrib>Paton, Alan</creatorcontrib><creatorcontrib>Simon, James E</creatorcontrib><title>Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers</title><title>Biochemical systematics and ecology</title><addtitle>Biochem Syst Ecol</addtitle><description>Morphological, chemical and genetic differences of 12 tree basil (
Ocimum gratissimum L.) accessions were studied to determine whether volatile oils and flavonoids can be used as taxonomical markers and to examine the relationship between RAPDs to these chemical markers. Eugenol, thymol, and geraniol were the major volatile oil constituents found in
Ocimum gratissimum. Xantomicrol and cirsimaritin were the major external flavones. The accessions morphologically described as
O. gratissimum var
. gratissimum contained eugenol as the major volatile oil constituent, and cirsimaritin as the major flavone.
Ocimum gratissimum var.
macrophyllum accessions contained thymol as the major volatile oil constituent, and xantomicrol as the major flavone. A distinct essential oil and flavone chemotype (producing geraniol and a mixture of the flavones cirsimaritin, isothymusin, xanthomicrol, and luteolin) was found in an accession genetically more distant from the other two groups when analyzed by molecular markers. The accessions could be divided based on volatile oil constituents into six groups: (1) thymol:
α-copaene (ot24, ot25, ot26, and ot28); (2) eugenol:spathulenol (ot17, ot63, and ot52); (3) thymol:
p-cymene (ot65); (4) eugenol:
γ-muurolene (ot27 and ot29); (5) eugenol:thymol: spathulenol (ot85); and (6) geraniol (ot84). Cluster analysis of RAPD markers showed that there are three groups that are distinct genetically and highly correlated (
r=0.814) to volatile oil constituents.</description><subject>a-copaene</subject><subject>basil</subject><subject>chemotypes</subject><subject>Cirsimaritin</subject><subject>cluster analysis</subject><subject>Essential oils</subject><subject>Eugenol</subject><subject>External flavones</subject><subject>flavonoids</subject><subject>g-muurolene</subject><subject>genetic markers</subject><subject>genetic variation</subject><subject>Geraniol</subject><subject>Lamiaceae</subject><subject>luteolin</subject><subject>Molecular markers</subject><subject>Ocimum gratissimum</subject><subject>oils</subject><subject>p-cymene</subject><subject>random amplified polymorphic DNA technique</subject><subject>spathulenol</subject><subject>Thymol</subject><subject>Tree basil</subject><subject>Xantomicrol</subject><issn>0305-1978</issn><issn>1873-2925</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkV1vFSEQhonR2GP1J6hcmZq4dWBh2b0yTVuryUlqrL0mLMw21N2lAnuS_ns5H9E7vYKEZx5m5iXkNYNTBqz5eAM1yIp1qj0BeA8ADa_EE7Jiraor3nH5lKz-IEfkRUr3BZKCq-fkiDEmeSfUitxf4YzZW-r8BmPy-ZGGgV5bPy0TvYsm-5R29_Up7U1CR8NMN2EsDyPS4Edqw5yyzwvOOX2gw2g2YQ7eJWpmR7-ffbugk4k_i_sleTaYMeGrw3lMbj9f_jj_Uq2vr76en60rK1qRK8Gd6BlXvAfRDMxYByiVrYENSg2Og-SyFq3iHePSMmgkb1ljDQgUqh9cfUxO9t6HGH4tmLKefLI4jmbGsCTNWtnVQhZBQd_9G1VtV6uuLaDcgzaGlCIO-iH6MtejZqC3eehdHnq7bA2gd3loUereHD5Y-gnd36pDAAV4uwcGE7S5iz7p2xtehMWgJBfbHj_tCSw723iMOlmPs0XnI9qsXfD_aeI3atWiZA</recordid><startdate>20010301</startdate><enddate>20010301</enddate><creator>Vieira, Roberto F</creator><creator>Grayer, Renée J</creator><creator>Paton, Alan</creator><creator>Simon, James E</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010301</creationdate><title>Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers</title><author>Vieira, Roberto F ; Grayer, Renée J ; Paton, Alan ; Simon, James E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-42d4b1272b046f1acd0e57c301f77fd20525348729125c10652816ca04e47bfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>a-copaene</topic><topic>basil</topic><topic>chemotypes</topic><topic>Cirsimaritin</topic><topic>cluster analysis</topic><topic>Essential oils</topic><topic>Eugenol</topic><topic>External flavones</topic><topic>flavonoids</topic><topic>g-muurolene</topic><topic>genetic markers</topic><topic>genetic variation</topic><topic>Geraniol</topic><topic>Lamiaceae</topic><topic>luteolin</topic><topic>Molecular markers</topic><topic>Ocimum gratissimum</topic><topic>oils</topic><topic>p-cymene</topic><topic>random amplified polymorphic DNA technique</topic><topic>spathulenol</topic><topic>Thymol</topic><topic>Tree basil</topic><topic>Xantomicrol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vieira, Roberto F</creatorcontrib><creatorcontrib>Grayer, Renée J</creatorcontrib><creatorcontrib>Paton, Alan</creatorcontrib><creatorcontrib>Simon, James E</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical systematics and ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vieira, Roberto F</au><au>Grayer, Renée J</au><au>Paton, Alan</au><au>Simon, James E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers</atitle><jtitle>Biochemical systematics and ecology</jtitle><addtitle>Biochem Syst Ecol</addtitle><date>2001-03-01</date><risdate>2001</risdate><volume>29</volume><issue>3</issue><spage>287</spage><epage>304</epage><pages>287-304</pages><issn>0305-1978</issn><eissn>1873-2925</eissn><abstract>Morphological, chemical and genetic differences of 12 tree basil (
Ocimum gratissimum L.) accessions were studied to determine whether volatile oils and flavonoids can be used as taxonomical markers and to examine the relationship between RAPDs to these chemical markers. Eugenol, thymol, and geraniol were the major volatile oil constituents found in
Ocimum gratissimum. Xantomicrol and cirsimaritin were the major external flavones. The accessions morphologically described as
O. gratissimum var
. gratissimum contained eugenol as the major volatile oil constituent, and cirsimaritin as the major flavone.
Ocimum gratissimum var.
macrophyllum accessions contained thymol as the major volatile oil constituent, and xantomicrol as the major flavone. A distinct essential oil and flavone chemotype (producing geraniol and a mixture of the flavones cirsimaritin, isothymusin, xanthomicrol, and luteolin) was found in an accession genetically more distant from the other two groups when analyzed by molecular markers. The accessions could be divided based on volatile oil constituents into six groups: (1) thymol:
α-copaene (ot24, ot25, ot26, and ot28); (2) eugenol:spathulenol (ot17, ot63, and ot52); (3) thymol:
p-cymene (ot65); (4) eugenol:
γ-muurolene (ot27 and ot29); (5) eugenol:thymol: spathulenol (ot85); and (6) geraniol (ot84). Cluster analysis of RAPD markers showed that there are three groups that are distinct genetically and highly correlated (
r=0.814) to volatile oil constituents.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11152947</pmid><doi>10.1016/S0305-1978(00)00062-4</doi><tpages>18</tpages></addata></record> |
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| issn | 0305-1978 1873-2925 |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | a-copaene basil chemotypes Cirsimaritin cluster analysis Essential oils Eugenol External flavones flavonoids g-muurolene genetic markers genetic variation Geraniol Lamiaceae luteolin Molecular markers Ocimum gratissimum oils p-cymene random amplified polymorphic DNA technique spathulenol Thymol Tree basil Xantomicrol |
| title | Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers |
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