Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein
A cDNA encoding a high-mobility-group protein has been isolated from a microspore-specific library of Brassica napus. The 930 bp cDNA contains a 612 bp open reading frame encoding a protein of 203 amino acids residues exhibiting significant homology to HMG-I/Y protein from Arabidopsis thaliana (62%)...
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| Veröffentlicht in: | Plant science (Limerick) 2000-11, Vol.159 (2), p.197-204 |
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| container_start_page | 197 |
| container_title | Plant science (Limerick) |
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| creator | Masek, Tomas Smykal, Petr Janotova, Iveta Honys, David Capkova, Vera Pechan, Paul M |
| description | A cDNA encoding a high-mobility-group protein has been isolated from a microspore-specific library of
Brassica napus. The 930 bp cDNA contains a 612 bp open reading frame encoding a protein of 203 amino acids residues exhibiting significant homology to HMG-I/Y protein from
Arabidopsis thaliana (62%). The predicted protein contains four copies of the ‘AT-hook’ motif which is involved in binding A/T-rich DNA. Southern blotting indicates that the HMG-I/Y gene is a single-copy gene in
B. napus. Transcription of the HMG-I/Y gene was detected in all tissues examined, with the highest expression in pollen-derived embryos. In situ localization studies of flower organs indicate the transcript to be preferentially located in petals and sepals. Subcellular localization analysis performed during pollen development showed that the transcript of the HMG-I/Y gene is predominantly associated with polysomes. |
| doi_str_mv | 10.1016/S0168-9452(00)00329-0 |
| format | Article |
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Brassica napus. The 930 bp cDNA contains a 612 bp open reading frame encoding a protein of 203 amino acids residues exhibiting significant homology to HMG-I/Y protein from
Arabidopsis thaliana (62%). The predicted protein contains four copies of the ‘AT-hook’ motif which is involved in binding A/T-rich DNA. Southern blotting indicates that the HMG-I/Y gene is a single-copy gene in
B. napus. Transcription of the HMG-I/Y gene was detected in all tissues examined, with the highest expression in pollen-derived embryos. In situ localization studies of flower organs indicate the transcript to be preferentially located in petals and sepals. Subcellular localization analysis performed during pollen development showed that the transcript of the HMG-I/Y gene is predominantly associated with polysomes.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/S0168-9452(00)00329-0</identifier><identifier>PMID: 11074272</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>AT-hook ; Brassica napus ; high mobility group protein I/Y ; HMG-I/Y gene ; HMG-I/Y protein ; Pollen ; Rape</subject><ispartof>Plant science (Limerick), 2000-11, Vol.159 (2), p.197-204</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-a3964a0f82112ba0f7319f58d2d895df6e1c9f6b6d4e99dd030f5ef8aab28c403</citedby><cites>FETCH-LOGICAL-c394t-a3964a0f82112ba0f7319f58d2d895df6e1c9f6b6d4e99dd030f5ef8aab28c403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0168-9452(00)00329-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11074272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masek, Tomas</creatorcontrib><creatorcontrib>Smykal, Petr</creatorcontrib><creatorcontrib>Janotova, Iveta</creatorcontrib><creatorcontrib>Honys, David</creatorcontrib><creatorcontrib>Capkova, Vera</creatorcontrib><creatorcontrib>Pechan, Paul M</creatorcontrib><title>Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein</title><title>Plant science (Limerick)</title><addtitle>Plant Sci</addtitle><description>A cDNA encoding a high-mobility-group protein has been isolated from a microspore-specific library of
Brassica napus. The 930 bp cDNA contains a 612 bp open reading frame encoding a protein of 203 amino acids residues exhibiting significant homology to HMG-I/Y protein from
Arabidopsis thaliana (62%). The predicted protein contains four copies of the ‘AT-hook’ motif which is involved in binding A/T-rich DNA. Southern blotting indicates that the HMG-I/Y gene is a single-copy gene in
B. napus. Transcription of the HMG-I/Y gene was detected in all tissues examined, with the highest expression in pollen-derived embryos. In situ localization studies of flower organs indicate the transcript to be preferentially located in petals and sepals. Subcellular localization analysis performed during pollen development showed that the transcript of the HMG-I/Y gene is predominantly associated with polysomes.</description><subject>AT-hook</subject><subject>Brassica napus</subject><subject>high mobility group protein I/Y</subject><subject>HMG-I/Y gene</subject><subject>HMG-I/Y protein</subject><subject>Pollen</subject><subject>Rape</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi0EokvLTwD5hMoh7diOE_uESunHSks50B44IMvxx9YoG6d2Uqn_Hm93BTe4zMzhmXlH74vQOwInBEhz-r0UUcma02OAjwCMygpeoAURLaso5fIlWvxBDtCbnH8BAOW8fY0OCIG2pi1doJ_LHHs9hTjg6LHGn5POORiNBz3OGa9OsPlyc4bdYKINw7oQ4zwV_tHh-7C-rzaxC32Ynqp1ivOIr79e4eXpDzymOLkwHKFXXvfZvd33Q3R3eXF7fl2tvl0tz89WlWGynirNZFNr8IISQrsytIxIz4WlVkhufeOIkb7pGls7Ka0FBp47L7TuqDA1sEN0vLtbdB9mlye1Cdm4vteDi3NWRHDJWNs0TUE__BtthSQSSAH5DjQp5pycV2MKG52eFAG1jUA9R6C2_ioA9RyB2v7yfi8wdxtn_27tPS_Apx3giiOPwSWVTSgGOxuSM5OyMfxH4je3F5Rk</recordid><startdate>20001106</startdate><enddate>20001106</enddate><creator>Masek, Tomas</creator><creator>Smykal, Petr</creator><creator>Janotova, Iveta</creator><creator>Honys, David</creator><creator>Capkova, Vera</creator><creator>Pechan, Paul M</creator><general>Elsevier Ireland Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20001106</creationdate><title>Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein</title><author>Masek, Tomas ; Smykal, Petr ; Janotova, Iveta ; Honys, David ; Capkova, Vera ; Pechan, Paul M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-a3964a0f82112ba0f7319f58d2d895df6e1c9f6b6d4e99dd030f5ef8aab28c403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>AT-hook</topic><topic>Brassica napus</topic><topic>high mobility group protein I/Y</topic><topic>HMG-I/Y gene</topic><topic>HMG-I/Y protein</topic><topic>Pollen</topic><topic>Rape</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masek, Tomas</creatorcontrib><creatorcontrib>Smykal, Petr</creatorcontrib><creatorcontrib>Janotova, Iveta</creatorcontrib><creatorcontrib>Honys, David</creatorcontrib><creatorcontrib>Capkova, Vera</creatorcontrib><creatorcontrib>Pechan, Paul M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masek, Tomas</au><au>Smykal, Petr</au><au>Janotova, Iveta</au><au>Honys, David</au><au>Capkova, Vera</au><au>Pechan, Paul M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2000-11-06</date><risdate>2000</risdate><volume>159</volume><issue>2</issue><spage>197</spage><epage>204</epage><pages>197-204</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><abstract>A cDNA encoding a high-mobility-group protein has been isolated from a microspore-specific library of
Brassica napus. The 930 bp cDNA contains a 612 bp open reading frame encoding a protein of 203 amino acids residues exhibiting significant homology to HMG-I/Y protein from
Arabidopsis thaliana (62%). The predicted protein contains four copies of the ‘AT-hook’ motif which is involved in binding A/T-rich DNA. Southern blotting indicates that the HMG-I/Y gene is a single-copy gene in
B. napus. Transcription of the HMG-I/Y gene was detected in all tissues examined, with the highest expression in pollen-derived embryos. In situ localization studies of flower organs indicate the transcript to be preferentially located in petals and sepals. Subcellular localization analysis performed during pollen development showed that the transcript of the HMG-I/Y gene is predominantly associated with polysomes.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>11074272</pmid><doi>10.1016/S0168-9452(00)00329-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant science (Limerick), 2000-11, Vol.159 (2), p.197-204 |
| issn | 0168-9452 1873-2259 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | AT-hook Brassica napus high mobility group protein I/Y HMG-I/Y gene HMG-I/Y protein Pollen Rape |
| title | Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein |
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