A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment
Abstract Recombinant derivatives of Pseudomonas putida strain KT2440 are of potential interest as microbial inoculants to be deliberately released for agricultural applications. To facilitate tracking of this strain and its derivatives after introduction into the environment, a mini-Tn5-′luxAB trans...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology ecology 2000-12, Vol.34 (2), p.91-102 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 102 |
|---|---|
| container_issue | 2 |
| container_start_page | 91 |
| container_title | FEMS microbiology ecology |
| container_volume | 34 |
| creator | Ramos, Cayo Molina, Lázaro Mølbak, Lars Ramos, Juan L. Molin, Søren |
| description | Abstract
Recombinant derivatives of Pseudomonas putida strain KT2440 are of potential interest as microbial inoculants to be deliberately released for agricultural applications. To facilitate tracking of this strain and its derivatives after introduction into the environment, a mini-Tn5-′luxAB transposon was introduced into the chromosome of P. putida KT2440, yielding strain P. putida S1B1. Sequencing of the DNA region located upstream of the ′luxAB genes and similarity search with the P. putida KT2440 genome sequence, localized the transposon within a 3021-bp open reading frame (ORF), whose translated sequence showed significant similarity with the hypothetical YdiJ proteins from Escherichia coli and Haemophilus influenzae. A second ORF adjacent to and divergent from the ydiJ sequence was also found and showed significant homology with various LysR-type transcriptional activator proteins from several bacteria. Disruption of the ydiJ locus in P. putida S1B1 did not affect the survival of the strain in unvegetated or vegetated soils. Bioluminescent detection of P. putida S1B1 cells enriched in selective media directly from soil allowed detection of culturable cells in soil samples over a period of at least 8 months. The addition of the luxAB biomarker facilitates tracking in the root system of several plant species grown under sterile and non-sterile conditions. The correlation of the bioluminescent phenotype with the growth activity of P. putida S1B1 cells colonizing the root system of barley and corn plants was estimated by monitoring ribosomal contents using quantitative hybridization with fluorescence-labeled ribosomal RNA probes. A correlation between inoculum density, light output, and ribosomal contents was found for P. putida cells colonizing the root system of barley seedlings grown under sterile conditions. Although ribosomal contents, and therefore growth activity, of P. putida S1B1 cells extracted from the rhizosphere of corn plants grown in non-sterile soil were similar to those found in starved cells, the luminescent system permitted non-destructive in situ detection of the strain in the upper root system. |
| doi_str_mv | 10.1111/j.1574-6941.2000.tb00758.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1859333108</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6941.2000.tb00758.x</oup_id><sourcerecordid>17756938</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4881-2d8fe11d93ec658ab47ca44ae2a8a122ebaeae29b6d000c722ef3e23fe60ee7e3</originalsourceid><addsrcrecordid>eNqVkV-L1DAUxYMo7rj6FSQoiC-t-dc0FXxYll0VV_RhfQ637S1maJMxacfdb2_KDKuICuYlJPmdk3s4hDzjrOR5vdqWvKpVoRvFS8EYK-eWsboy5c09srl7uk82jGtTaNXoE_IopS1jvJKKPSQn2YUJbfSG9Ge0dWFcJucxdehn2mN0e5jdHmkY6OeESx-m4CHR3TK7HuiHa6EUo0OImR1dixFmpBFHhITU-TnQ-StS9HsXg5-y52PyYIAx4ZPjfkq-XF5cn78rrj69fX9-dlV0yhheiN4MyHnfSOx0ZaBVdQdKAQowwIXAFjAfmlb3OXVX55tBopADaoZYozwlLw--uxi-LZhmO7kcahzBY1iS5aZqpJScmYy--Dda15Vu5Ao-_w3chiX6HMOKbGXWRppMvT5QXQwpRRzsLroJ4q3lzK6I3dq1Gbs2Y9fS7LE0e5PFT49fLO2E_U_psaVfZoDUwThE8J1Ld5xRTFciU28O1Hc34u1_DGAvLz42POurgz4su7-oiz-N_wOX0cM9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2331811119</pqid></control><display><type>article</type><title>A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment</title><source>Oxford Journals Open Access Collection</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Ramos, Cayo ; Molina, Lázaro ; Mølbak, Lars ; Ramos, Juan L. ; Molin, Søren</creator><creatorcontrib>Ramos, Cayo ; Molina, Lázaro ; Mølbak, Lars ; Ramos, Juan L. ; Molin, Søren</creatorcontrib><description>Abstract
Recombinant derivatives of Pseudomonas putida strain KT2440 are of potential interest as microbial inoculants to be deliberately released for agricultural applications. To facilitate tracking of this strain and its derivatives after introduction into the environment, a mini-Tn5-′luxAB transposon was introduced into the chromosome of P. putida KT2440, yielding strain P. putida S1B1. Sequencing of the DNA region located upstream of the ′luxAB genes and similarity search with the P. putida KT2440 genome sequence, localized the transposon within a 3021-bp open reading frame (ORF), whose translated sequence showed significant similarity with the hypothetical YdiJ proteins from Escherichia coli and Haemophilus influenzae. A second ORF adjacent to and divergent from the ydiJ sequence was also found and showed significant homology with various LysR-type transcriptional activator proteins from several bacteria. Disruption of the ydiJ locus in P. putida S1B1 did not affect the survival of the strain in unvegetated or vegetated soils. Bioluminescent detection of P. putida S1B1 cells enriched in selective media directly from soil allowed detection of culturable cells in soil samples over a period of at least 8 months. The addition of the luxAB biomarker facilitates tracking in the root system of several plant species grown under sterile and non-sterile conditions. The correlation of the bioluminescent phenotype with the growth activity of P. putida S1B1 cells colonizing the root system of barley and corn plants was estimated by monitoring ribosomal contents using quantitative hybridization with fluorescence-labeled ribosomal RNA probes. A correlation between inoculum density, light output, and ribosomal contents was found for P. putida cells colonizing the root system of barley seedlings grown under sterile conditions. Although ribosomal contents, and therefore growth activity, of P. putida S1B1 cells extracted from the rhizosphere of corn plants grown in non-sterile soil were similar to those found in starved cells, the luminescent system permitted non-destructive in situ detection of the strain in the upper root system.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2000.tb00758.x</identifier><identifier>PMID: 11102686</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Barley ; Biochemistry and biology ; Biological and medical sciences ; Biomarkers ; Biotechnology ; Chemical, physicochemical, biochemical and biological properties ; Chromosomes ; Corn ; Deliberate release ; Deoxyribonucleic acid ; Derivatives ; Disruption ; DNA ; DNA probes ; DNA sequencing ; E coli ; Ecology ; Environment and pollution ; environmental monitoring ; Fluorescence ; Fluorescent indicators ; Fundamental and applied biological sciences. Psychology ; General agronomy. Plant production ; Genetically engineered microorganism ; Genetically engineered organisms behavior (microorganisms, plants, animals) ; Genomes ; Homology ; Hybridization ; Industrial applications and implications. Economical aspects ; Inoculum ; luxAB ; Microbiology ; Microorganisms ; Nucleotide sequence ; Open reading frames ; Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries ; Phenotypes ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; Plant species ; Proteins ; Pseudomonas putida ; Pseudomonas putida KT2440 ; Rhizosphere ; RNA probes ; Roots ; rRNA ; Seedlings ; Selective media ; Similarity ; Soil science ; Soil-plant relationships. Soil fertility. Fertilization. Amendments ; Soils ; Tracking ; Transcription ; Transposons</subject><ispartof>FEMS microbiology ecology, 2000-12, Vol.34 (2), p.91-102</ispartof><rights>2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2000</rights><rights>2001 INIST-CNRS</rights><rights>2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4881-2d8fe11d93ec658ab47ca44ae2a8a122ebaeae29b6d000c722ef3e23fe60ee7e3</citedby><cites>FETCH-LOGICAL-c4881-2d8fe11d93ec658ab47ca44ae2a8a122ebaeae29b6d000c722ef3e23fe60ee7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2000.tb00758.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2000.tb00758.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=840652$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11102686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramos, Cayo</creatorcontrib><creatorcontrib>Molina, Lázaro</creatorcontrib><creatorcontrib>Mølbak, Lars</creatorcontrib><creatorcontrib>Ramos, Juan L.</creatorcontrib><creatorcontrib>Molin, Søren</creatorcontrib><title>A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
Recombinant derivatives of Pseudomonas putida strain KT2440 are of potential interest as microbial inoculants to be deliberately released for agricultural applications. To facilitate tracking of this strain and its derivatives after introduction into the environment, a mini-Tn5-′luxAB transposon was introduced into the chromosome of P. putida KT2440, yielding strain P. putida S1B1. Sequencing of the DNA region located upstream of the ′luxAB genes and similarity search with the P. putida KT2440 genome sequence, localized the transposon within a 3021-bp open reading frame (ORF), whose translated sequence showed significant similarity with the hypothetical YdiJ proteins from Escherichia coli and Haemophilus influenzae. A second ORF adjacent to and divergent from the ydiJ sequence was also found and showed significant homology with various LysR-type transcriptional activator proteins from several bacteria. Disruption of the ydiJ locus in P. putida S1B1 did not affect the survival of the strain in unvegetated or vegetated soils. Bioluminescent detection of P. putida S1B1 cells enriched in selective media directly from soil allowed detection of culturable cells in soil samples over a period of at least 8 months. The addition of the luxAB biomarker facilitates tracking in the root system of several plant species grown under sterile and non-sterile conditions. The correlation of the bioluminescent phenotype with the growth activity of P. putida S1B1 cells colonizing the root system of barley and corn plants was estimated by monitoring ribosomal contents using quantitative hybridization with fluorescence-labeled ribosomal RNA probes. A correlation between inoculum density, light output, and ribosomal contents was found for P. putida cells colonizing the root system of barley seedlings grown under sterile conditions. Although ribosomal contents, and therefore growth activity, of P. putida S1B1 cells extracted from the rhizosphere of corn plants grown in non-sterile soil were similar to those found in starved cells, the luminescent system permitted non-destructive in situ detection of the strain in the upper root system.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Barley</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Chromosomes</subject><subject>Corn</subject><subject>Deliberate release</subject><subject>Deoxyribonucleic acid</subject><subject>Derivatives</subject><subject>Disruption</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA sequencing</subject><subject>E coli</subject><subject>Ecology</subject><subject>Environment and pollution</subject><subject>environmental monitoring</subject><subject>Fluorescence</subject><subject>Fluorescent indicators</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General agronomy. Plant production</subject><subject>Genetically engineered microorganism</subject><subject>Genetically engineered organisms behavior (microorganisms, plants, animals)</subject><subject>Genomes</subject><subject>Homology</subject><subject>Hybridization</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Inoculum</subject><subject>luxAB</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Nucleotide sequence</subject><subject>Open reading frames</subject><subject>Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries</subject><subject>Phenotypes</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Plant species</subject><subject>Proteins</subject><subject>Pseudomonas putida</subject><subject>Pseudomonas putida KT2440</subject><subject>Rhizosphere</subject><subject>RNA probes</subject><subject>Roots</subject><subject>rRNA</subject><subject>Seedlings</subject><subject>Selective media</subject><subject>Similarity</subject><subject>Soil science</subject><subject>Soil-plant relationships. Soil fertility. Fertilization. Amendments</subject><subject>Soils</subject><subject>Tracking</subject><subject>Transcription</subject><subject>Transposons</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqVkV-L1DAUxYMo7rj6FSQoiC-t-dc0FXxYll0VV_RhfQ637S1maJMxacfdb2_KDKuICuYlJPmdk3s4hDzjrOR5vdqWvKpVoRvFS8EYK-eWsboy5c09srl7uk82jGtTaNXoE_IopS1jvJKKPSQn2YUJbfSG9Ge0dWFcJucxdehn2mN0e5jdHmkY6OeESx-m4CHR3TK7HuiHa6EUo0OImR1dixFmpBFHhITU-TnQ-StS9HsXg5-y52PyYIAx4ZPjfkq-XF5cn78rrj69fX9-dlV0yhheiN4MyHnfSOx0ZaBVdQdKAQowwIXAFjAfmlb3OXVX55tBopADaoZYozwlLw--uxi-LZhmO7kcahzBY1iS5aZqpJScmYy--Dda15Vu5Ao-_w3chiX6HMOKbGXWRppMvT5QXQwpRRzsLroJ4q3lzK6I3dq1Gbs2Y9fS7LE0e5PFT49fLO2E_U_psaVfZoDUwThE8J1Ld5xRTFciU28O1Hc34u1_DGAvLz42POurgz4su7-oiz-N_wOX0cM9</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Ramos, Cayo</creator><creator>Molina, Lázaro</creator><creator>Mølbak, Lars</creator><creator>Ramos, Juan L.</creator><creator>Molin, Søren</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200012</creationdate><title>A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment</title><author>Ramos, Cayo ; Molina, Lázaro ; Mølbak, Lars ; Ramos, Juan L. ; Molin, Søren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4881-2d8fe11d93ec658ab47ca44ae2a8a122ebaeae29b6d000c722ef3e23fe60ee7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Barley</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Chromosomes</topic><topic>Corn</topic><topic>Deliberate release</topic><topic>Deoxyribonucleic acid</topic><topic>Derivatives</topic><topic>Disruption</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA sequencing</topic><topic>E coli</topic><topic>Ecology</topic><topic>Environment and pollution</topic><topic>environmental monitoring</topic><topic>Fluorescence</topic><topic>Fluorescent indicators</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General agronomy. Plant production</topic><topic>Genetically engineered microorganism</topic><topic>Genetically engineered organisms behavior (microorganisms, plants, animals)</topic><topic>Genomes</topic><topic>Homology</topic><topic>Hybridization</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Inoculum</topic><topic>luxAB</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Nucleotide sequence</topic><topic>Open reading frames</topic><topic>Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries</topic><topic>Phenotypes</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Plant species</topic><topic>Proteins</topic><topic>Pseudomonas putida</topic><topic>Pseudomonas putida KT2440</topic><topic>Rhizosphere</topic><topic>RNA probes</topic><topic>Roots</topic><topic>rRNA</topic><topic>Seedlings</topic><topic>Selective media</topic><topic>Similarity</topic><topic>Soil science</topic><topic>Soil-plant relationships. Soil fertility. Fertilization. Amendments</topic><topic>Soils</topic><topic>Tracking</topic><topic>Transcription</topic><topic>Transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramos, Cayo</creatorcontrib><creatorcontrib>Molina, Lázaro</creatorcontrib><creatorcontrib>Mølbak, Lars</creatorcontrib><creatorcontrib>Ramos, Juan L.</creatorcontrib><creatorcontrib>Molin, Søren</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramos, Cayo</au><au>Molina, Lázaro</au><au>Mølbak, Lars</au><au>Ramos, Juan L.</au><au>Molin, Søren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2000-12</date><risdate>2000</risdate><volume>34</volume><issue>2</issue><spage>91</spage><epage>102</epage><pages>91-102</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
Recombinant derivatives of Pseudomonas putida strain KT2440 are of potential interest as microbial inoculants to be deliberately released for agricultural applications. To facilitate tracking of this strain and its derivatives after introduction into the environment, a mini-Tn5-′luxAB transposon was introduced into the chromosome of P. putida KT2440, yielding strain P. putida S1B1. Sequencing of the DNA region located upstream of the ′luxAB genes and similarity search with the P. putida KT2440 genome sequence, localized the transposon within a 3021-bp open reading frame (ORF), whose translated sequence showed significant similarity with the hypothetical YdiJ proteins from Escherichia coli and Haemophilus influenzae. A second ORF adjacent to and divergent from the ydiJ sequence was also found and showed significant homology with various LysR-type transcriptional activator proteins from several bacteria. Disruption of the ydiJ locus in P. putida S1B1 did not affect the survival of the strain in unvegetated or vegetated soils. Bioluminescent detection of P. putida S1B1 cells enriched in selective media directly from soil allowed detection of culturable cells in soil samples over a period of at least 8 months. The addition of the luxAB biomarker facilitates tracking in the root system of several plant species grown under sterile and non-sterile conditions. The correlation of the bioluminescent phenotype with the growth activity of P. putida S1B1 cells colonizing the root system of barley and corn plants was estimated by monitoring ribosomal contents using quantitative hybridization with fluorescence-labeled ribosomal RNA probes. A correlation between inoculum density, light output, and ribosomal contents was found for P. putida cells colonizing the root system of barley seedlings grown under sterile conditions. Although ribosomal contents, and therefore growth activity, of P. putida S1B1 cells extracted from the rhizosphere of corn plants grown in non-sterile soil were similar to those found in starved cells, the luminescent system permitted non-destructive in situ detection of the strain in the upper root system.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11102686</pmid><doi>10.1111/j.1574-6941.2000.tb00758.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-6496 |
| ispartof | FEMS microbiology ecology, 2000-12, Vol.34 (2), p.91-102 |
| issn | 0168-6496 1574-6941 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1859333108 |
| source | Oxford Journals Open Access Collection; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Agronomy. Soil science and plant productions Barley Biochemistry and biology Biological and medical sciences Biomarkers Biotechnology Chemical, physicochemical, biochemical and biological properties Chromosomes Corn Deliberate release Deoxyribonucleic acid Derivatives Disruption DNA DNA probes DNA sequencing E coli Ecology Environment and pollution environmental monitoring Fluorescence Fluorescent indicators Fundamental and applied biological sciences. Psychology General agronomy. Plant production Genetically engineered microorganism Genetically engineered organisms behavior (microorganisms, plants, animals) Genomes Homology Hybridization Industrial applications and implications. Economical aspects Inoculum luxAB Microbiology Microorganisms Nucleotide sequence Open reading frames Other nutrients. Amendments. Solid and liquid wastes. Sludges and slurries Phenotypes Physics, chemistry, biochemistry and biology of agricultural and forest soils Plant species Proteins Pseudomonas putida Pseudomonas putida KT2440 Rhizosphere RNA probes Roots rRNA Seedlings Selective media Similarity Soil science Soil-plant relationships. Soil fertility. Fertilization. Amendments Soils Tracking Transcription Transposons |
| title | A bioluminescent derivative of Pseudomonas putida KT2440 for deliberate release into the environment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T20%3A41%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20bioluminescent%20derivative%20of%20Pseudomonas%20putida%20KT2440%20for%20deliberate%20release%20into%20the%20environment&rft.jtitle=FEMS%20microbiology%20ecology&rft.au=Ramos,%20Cayo&rft.date=2000-12&rft.volume=34&rft.issue=2&rft.spage=91&rft.epage=102&rft.pages=91-102&rft.issn=0168-6496&rft.eissn=1574-6941&rft_id=info:doi/10.1111/j.1574-6941.2000.tb00758.x&rft_dat=%3Cproquest_cross%3E17756938%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2331811119&rft_id=info:pmid/11102686&rft_oup_id=10.1111/j.1574-6941.2000.tb00758.x&rfr_iscdi=true |